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Characterization of Pv92, a Novel Merozoite Surface Protein of Plasmodium vivax

  • Lee, Seong-Kyun;Wang, Bo;Han, Jin-Hee;Nyunt, Myat Htut;Muh, Fauzi;Chootong, Patchanee;Ha, Kwon-Soo;Park, Won Sun;Hong, Seok-Ho;Park, Jeong-Hyun;Han, Eun-Taek
    • Parasites, Hosts and Diseases
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    • v.54 no.4
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    • pp.385-391
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    • 2016
  • The discovery and understanding of antigenic proteins are essential for development of a vaccine against malaria. In Plasmodium falciparum, Pf92 have been characterized as a merozoite surface protein, and this protein is expressed at the late schizont stage, but no study of Pv92, the orthologue of Pf92 in P. vivax, has been reported. Thus, the protein structure of Pv92 was analyzed, and the gene sequence was aligned with that of other Plasmodium spp. using bioinformatics tools. The recombinant Pv92 protein was expressed and purified using bacterial expression system and used for immunization of mice to gain the polyclonal antibody and for evaluation of antigenicity by protein array. Also, the antibody against Pv92 was used for subcellular analysis by immunofluorescence assay. The Pv92 protein has a signal peptide and a sexual stage s48/45 domain, and the cysteine residues at the N-terminal of Pv92 were completely conserved. The N-terminal of Pv92 was successfully expressed as soluble form using a bacterial expression system. The antibody raised against Pv92 recognized the parasites and completely merged with PvMSP1-19, indicating that Pv92 was localized on the merozoite surface. Evaluation of the human humoral immune response to Pv92 indicated moderate antigenicity, with 65% sensitivity and 95% specificity by protein array. Taken together, the merozoite surface localization and antigenicity of Pv92 implicate that it might be involved in attachment and invasion of a merozoite to a new host cell or immune evasion during invasion process.

Temperature Measurement of the Contact Surface from the Analysis of Temperature Distribution of the Hot Spots (열원의 온도분포 해석을 통한 접촉표면의 온도측정)

  • 정동윤
    • Tribology and Lubricants
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    • v.9 no.1
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    • pp.16-21
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    • 1993
  • A method has been developed to measure the surface temperature in a sliding tribosystem. The determination of the surface temperature was inferred from the temperature of hot spots which were generated by frictional heat. The temperature of hot spots was determined by regressing those digitized data on Gecim-Winer's theoretical model. The experimental results are discussed considering the important factors such as PV and frictional heat. The surface temperature rise is related to the thermal conductivity in low PV range. As PV increases, it reaches nearly constant value called the critical temperature.

Analysis of Performance of Building Integrated PV System of Cold Facade type (Cold facade형 BIPV시스템의 발전성능 분석)

  • Kim, Hyun-II;Kang, Gi-Hwan;Park, Kyung-Eun;Yu, Gwon-Jong;Shu, Seung-Jik
    • 한국태양에너지학회:학술대회논문집
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    • 2008.04a
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    • pp.275-280
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    • 2008
  • Photovoltaic(PV) permit the on-site production of electricity without concern for fuel supply or environmental adverse effects. The electrical power is produced without noise and little depletion of resources. So BIPV(Building-Integrated Photovoltaic) system have been increased around the world. Hereby the relative installation costs of the system will be relatively low compared to traditional installations of PV in high-rise buildings. This paper examined possibility of BIPV system of cold facade type and analyzed of performance of BIPV system of cold facade type. The system is influenced by conditions such as irradiation, module temperature, shade and architectural component etc. If this BIPV system of 1.1kW is possible the natural ventilation in the summer case, the temperature of PV module decrease and then the efficiency of PV system increase generally. By the results, the annual averaged PR of BIPV system of cold facade type is about 73.1%.

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Differential Induction of Pathogenesis-Related Proteins in the Compatible and Incompatible Interactions of Tomato Leaves with Xanthomonas campestris pv. vesicatoria (Xanthomonas campestris pv. vesicatoria와 토마토잎의 친화적, 불친화적 반응에서 병생성관련 단백질의 유도)

  • 김정동;황병국
    • Korean Journal Plant Pathology
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    • v.11 no.1
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    • pp.53-60
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    • 1995
  • Inoculation with the compatible strain Ds 1 of Xanthomonas campestris pv. vesicatoria caused brownish ad water-soaked lesions, but incompatible strain Bv5-4a produced hypersensitive symptoms with local necrosis on tomato (cv. Kwangyang) leaves. Bacterial populations of the compatible strains Ds 1 propagated more greatly than the incompatible strain Bv5-4a at the frist onset, but no differences were observed 5 days after inoculation. The bacterial infection induced the synthesis and accumulation of soluble proteins in tomato leaves, especially in the incompatible interaction. Native-polyacrylamide gel electrophoresis distinguished the soluble proteins in the tomato leaves infected by the compatible or incompatible strains. A protein of low molecular weight occurred only in the incompatible interaction. Some pathogenesis-related (PR) proteins, especially the 15, 18, 23, 26 and 54 kDa proteins, were detected only in the infected tomato leaves. In the two-dimensional electrophoresis, some proteins with different molecular weights (Mr. 21∼29 kDa) and the pI 8∼9 appeared more distinctly only in the incompatible interaction. These data suggest that the de novo synthesis of some PR proteins in tomato may be significant in defense against X. c. pv. vesicatoria.

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Water Extract from Spent Mushroom Substrate of Hericium erinaceus Suppresses Bacterial Wilt Disease of Tomato

  • Kwak, A Min;Min, Kyeong Jin;Lee, Sang Yeop;Kang, Hee Wan
    • Mycobiology
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    • v.43 no.3
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    • pp.311-318
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    • 2015
  • Culture filtrates of six different edible mushroom species were screened for antimicrobial activity against tomato wilt bacteria Ralstonia solanacearum B3. Hericium erinaceus, Lentinula edodes (Sanjo 701), Grifola frondosa, and Hypsizygus marmoreus showed antibacterial activity against the bacteria. Water, n-butanol, and ethyl acetate extracts of spent mushroom substrate (SMS) of H. erinaceus exhibited high antibacterial activity against different phytopathogenic bacteria: Pectobacterium carotovorum subsp. carotovorum, Agrobacterium tumefaciens, R. solanacearum, Xanthomonas oryzae pv. oryzae, X. campestris pv. campestris, X. axonopodis pv. vesicatoria, X. axonopodis pv. citiri, and X. axonopodis pv. glycine. Quantitative real-time PCR revealed that water extracts of SMS (WESMS) of H. erinaceus induced expressions of plant defense genes encoding ${\beta}$-1,3-glucanase (GluA) and pathogenesis-related protein-1a (PR-1a), associated with systemic acquired resistance. Furthermore, WESMS also suppressed tomato wilt disease caused by R. solanacearum by 85% in seedlings and promoted growth (height, leaf number, and fresh weight of the root and shoot) of tomato plants. These findings suggest the WESMS of H. erinaceus has the potential to suppress bacterial wilt disease of tomato through multiple effects including antibacterial activity, plant growth promotion, and defense gene induction.

Improving Energy Self-sufficiency in Municipal Wastewater Treatment Plant using Renewable Energy Production (능동적 신재생에너지 생산을 통한 하수처리장 에너지자립화 향상)

  • Kang, Ji-Hoon;Chae, Kyu-Jung;Kim, Dong-Soo;Yang, Hee-Jung;An, Yeong-Seop;Kim, Won-Kyoung;Kim, Jeong-Hyeon;Park, Dong-Eul
    • Proceedings of the Korea Water Resources Association Conference
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    • 2012.05a
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    • pp.643-643
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    • 2012
  • Increasing energy prices and growing concerns about global warming address the need to improve energy self-sufficiency in many industrial and municipal sectors. Wastewater treatment plants (WWTPs) are representative of energy-consuming facilities in Korea, accounting for 5% of national energy consumption. We present renewable energy technologies and energy self-sufficiency scenarios in a municipal WWTP ($30,000m^3d^{-1}$) located in Yongin, South Korea. By employing photovoltaics (PV, 135 kW), small hydropower turbine (10 kW), and thermal energy from treated effluent (25 RT: refrigeration ton) within the WWTP, a total of 142 tonne of oil equivalent (toe) of energy was estimated to be generated, accounting for $365ton\;CO_2\;yr^{-1}$ of greenhouse gas emission reduction. Core renewable technologies under consideration include 1) hybrid solar PV system consisting of fixed PV, dual-axis PV, and building integrated PV, 2) low-head small hydropower plant specifically designed for treated effluent, 3) effluent heat recovery system for heating and air conditioning. In addition to these core technologies, smart operation and management scheme will be presented for enhancing overall energy savings and distribution within the WWTP.

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Fabrication of Shingled Design Bifacial c-Si Photovoltaic Modules (슁글드 디자인 고출력 양면수광형 단결정 실리콘 태양광 모듈 제작)

  • Park, Min-Joon;Kim, Minseob;Shin, Jinho;Byeon, Su-Bin;Jeong, Chaehwan
    • Current Photovoltaic Research
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    • v.10 no.1
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    • pp.1-5
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    • 2022
  • Bifacial photovoltaic (PV) technology has received considerable attention in recent years due to the potential to achieve a higher annual energy yield compared to its monofacial PV systems. In this study, we fabricated the bifacial c-Si PV module with a shingled design using the conventional patterned bifacial solar cells. The shingled design PV module has recently attracted attention as a high-power module. Compared to the conventional module, it can have a much more active area due to the busbar-free structure. We employed the transparent backsheet for a light reception at the rear side of the PV module. Finally, we achieved a conversion power of 453.9 W for a 1300 mm × 2000 mm area. Moreover, we perform reliability tests to verify the durability of our Shingled Design Bifacial c-Si Photovoltaic module.

Application of Synergistic Solvent Extraction by Formation of Ternary Complex for Determination of Trace Zn(II) in Water Samples

  • Choi, Jong-Moon;Park, Hyun-Mo;Choi, Sun-Do
    • Bulletin of the Korean Chemical Society
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    • v.27 no.4
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    • pp.563-567
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    • 2006
  • The application of a synergistic solvent extraction by the formation of ternary complex with pyrocatechol violet (PV) and benzalkonium chloride (BC) was studied for determination of trace Zn(II) in water samples. The pH of sample solution and the amount of PV and BC added were optimized for the formation of the stable complex, a proper solvent was selected for the effective extraction, and the concentration of nitric acid was fixed for the back extraction of the complex from the solvent. After the ionic strength of 100 mL sample solution was adjusted to 0.1 M by adding NaCl and the pH was fixed at 9 with a carbonate buffer, 1.0 mL of 2% PV solution was added to form Zn(II)-PV complex then the Zn(II)-PV/BC ternary complex was made by adding 1.0 mL of 10% BC solution. The ternary complex was extracted into 10 mL of MIBK. And the ternary complex was back-extracted with 10 mL of 1.0 mol/L nitric acid to determine Zn(II) by a flame atomic absorption spectrophotometer (flame-AAS). The interference of concomitant ions on the extraction of Zn(II) was investigated. This procedure was applied to the analysis of three real samples such as Dalbang-dam water, laboratory tap water and Jungnajin seawater. The recoveries of Zn(II) in spiked samples were 86.58-104.1%.

A Study on Disposal Diagnosis Algorithm of PV Modules Considering Performance Degradation Rate (태양광모듈의 성능저하율을 고려한 폐기진단 알고리즘에 관한 연구)

  • Park, Ji-Hyun;Lee, Hu-Dong;Tae, Dong-Hyun;Ferreira, Marito;Rho, Dae-Seok
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.10
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    • pp.493-502
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    • 2019
  • Recently, the installation of renewable energy including PV systems in distribution systems has increased energetically to cope with climate change and energy shortages according to the government's policy of renewable energy 3020. On the other hand, the electrical performance and lifespan of PV modules installed outdoors can be decreased considerably due to a range of deterioration phenomena depending on the ambient environmental factors. To overcome these problems, replacement of degraded PV modules with new ones is increased before the lifespan guaranteed by the makers of PV modules. Therefore, this paper proposes a disposal diagnosis algorithm to evaluate the time interval of the optimal replacement for PV modules according to performance degradation rate of PV modules. In addition, this study modeled an economic evaluation, which is composed of the cost and benefit of PV systems. From the simulation results based on the proposed modeling and algorithm to consider the performance degradation rate specifically, the replacement approach was found to have the best B/C ratio in 10th year with a 3[%] degradation rate and the disposal diagnosis algorithm of PV modules are useful and practical tools for economic evaluations of the replacement of PV modules.

A Study on Degradation Phenomenon Based on Test Device for Aging Diagnosis in PV Modules (태양광모듈의 열화진단 시험장치 구현 및 열화특성에 관한 연구)

  • Shen, Jian;Lee, Hu-Dong;Tae, Dong-Hyun;Rho, Dae-Seok
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.6
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    • pp.27-35
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    • 2021
  • Generally, a proper evaluation method of the aging phenomenon of PV modules is required as the electrical performance and lifespan of PV modules can degrade significantly due to several environmental factors, while they are generally known as devices that are used semi-permanently for more than 20 years. On the other hand, there is a lack of objectivity in the existing evaluation method of the aging phenomenon, which compares the adjusted PV output based on STC with the initial PV module specifications due to the data distortion while adjusting the measured data. Therefore, this study implemented a test device for an aging diagnosis to measure and collect actual data from a PV module section and modeled the data for aging using MATLAB S/W to minimize the variability of the PV output, communication error, and delay. Furthermore, this study confirmed the usefulness of the presented test device for aging diagnosis of the PV modules by diagnosing the total period and yearly-basis degradation rate of aging PV modules as 25.73% and 1.55%, respectively, according to the on-site output characteristics of the PV modules by season.