• Title/Summary/Keyword: PS-2

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Characteristics of Photosynthetic Electron Transport Activity in Isolated Chloroplast of Korean Ginseng and Radish (인삼과 무 엽록체의 광합성 전자전달 활성)

  • 김갑식
    • Journal of Plant Biology
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    • v.33 no.2
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    • pp.111-118
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    • 1990
  • In order to characterize the chloroplasts of Korean ginseng as a semi-shade plant and radish as a sun plant, effects of growth light intensity on photosynthetic electron transport (PS) activity in chloroplasts and superoxide (O2.-) production in thylakoid membrane by irradiation were investigated. High-light chloroplasts of both plants showed higher PS activities than those grown under ow growth light intensity. High PS II and low PS I activities in ginseng chloroplasts (ratio of PS II/PS I : 1.1) were observed, but radish chloroplasts showed low PS II and high PS I activities (ratio of PS II/PS I : 0.3). PS II activity of both plants was little affected by temperature in range of 15-35$^{\circ}C$. Activities of whole -chain (PS II+I) in ginseng and PS I in radish were increased at high temperature (4$0^{\circ}C$). Preincubation of chloroplasts at 4$0^{\circ}C$ during 30 min, as a mild heat stress, caused rapid decrease in PS II and PS II+I activities of both plants. However PS I activity was not decreased in ginseng and rather increased in radish. O2.- production (NBT reduction) in Mehler reaction in the thylakoid membrane was inhibited by DCMU in both plants. DMBIB inhibited O2.- production in ginseng, but radish was insensitive to DMBIB. Electron flow system in ginseng thylakoid membrane was more susceptible to damage of photooxidation than that of radish.

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Studies on the fluorescent Pseudomoηas isolated from the wheat rhizosphere (소맥근권(小麥根圈)에서 분리(分離)한 형광성(螢光性) Pseudomonas spp. 에 관(關)한 연구(硏究))

  • Lee, Myung-Chol;Kim, Yong-Woong;Kim, Kwang-Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.23 no.2
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    • pp.152-159
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    • 1990
  • Four active strains, producing siderophore and antagonizing to soil plant disease fungi, were isolated and identified from the wheat rhizosphere to reduce the injurious effects of continuous cropping and study on the biological control. The obtained results were summerized as follow. 1. Four strains of fluorescent Pseudomonas were isolated from the wheat rhizosphere and identified as Pseudomonas fluorescens Biotype A(Ps-1,2,5) and Biotype B(Ps-3) That strains inhibitied growth of R. solani, F.oxysperum and F. solani in vitro 2. Optical density of pigment was maximum at 410nm. 3. Siderophore production by identified strains was decreased with addition of $Fe^{+3}$, although not decreased with addition of $Fe^{+2}$ 4. Pigment of Ps-1, 2 and 3 strain inhibitied growth of R. slani, F. oxysperum and F. solani but pigment of Ps-5 strain did not inhibitie growth of R. solani 5. Effect of inoculation was in order of Ps-2, 1, 5 and 3 strain through the dark culture method, and effect of Ps-1 and Ps-2 was greater than that of treatment of captan 50ppm.

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Effects of Pelleted Sugarcane Tops on Voluntary Feed Intake, Digestibility and Rumen Fermentation in Beef Cattle

  • Yuangklang, Chalermpon;Wanapat, M.;Wachirapakorn, C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.1
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    • pp.22-26
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    • 2005
  • Four male crossbred beef steers about 2 years old were used in a 4$\{times}$4 Latin square design to investigate the effect of pelleted sugarcane tops on voluntary feed intake, rumen fermentation and digestibility of nutrients. Experimental treatments were; Control (dried-chopped sugarcane tops (DCST)); PS1 (Pelleted sugarcane tops at 1 cm of diameter); PS2 (Pelleted sugarcane tops at 2 cm of diameter) and PS3 (Pelleted sugarcane tops at 3 cm of diameter). Roughage intake and total dry matter intake were 1.59, 1.62, 1.61, 1.63% BW and 2.09, 2.12, 2.11 and 2.13% BW in control, PS1, PS2 and PS3 treatments, respectively (p<0.05). Digestibility of DM, OM and CP were similar in control and PS3 treatment but there was significant difference (p<0.05) between control and PS1, PS2 treatments. Digestibility of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were 52.89, 50.01, 50.05 and 50.56% and 41.91, 39.96, 39.91 and 39.69% in control, PS1, PS2 and PS3, respectively (p<0.05). Total volatile fatty acids concentrations in rumen contents was 67.68, 65.93, 66.15 and 66.67 mM in control, PS1, PS2 and PS3, respectively (p<0.05). Even though, concentrations of acetate and butyrate (%) were significant different (p<0.05) but concentration of propionate (%) was not affected by treatments (p>0.05). Rumen pH, ammonia nitrogen and plasma urea nitrogen were significantly different (p<0.05) among treatments. From this experiment, it was found that dried-chopped sugarcane tops increased digestibility of nutrients whereas pelleted sugarcane tops increased feed intake in beef cattle. However, pelleted sugarcane tops at 3 cm of diameter did similar result in digestibility and rumen parameters with DCST. Therefore, it could be concluded that pelleting sugarcane top is an alternative way to improve the quality of sugarcane tops for use as ruminant roughage source.

Preparation of Novel PS-zeolite Beads Immobilized Zeolite with Polysulfone for Radioactive Materials (Polysulfone으로 제올라이트 A를 고정화한 방사성 물질제거용 PS-zeolite 비드 제조)

  • Lee, Chang-Han;Park, Jeong-Min;Kam, Sang-Kyu;Lee, Min-Gyu
    • Journal of Korean Society of Environmental Engineers
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    • v.37 no.3
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    • pp.145-151
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    • 2015
  • In order to remove Sr ions and Cs ions from aqueous solution, PS-zeolite beads were prepared by immobilizing zeolite with polysulfone (PS). The prepared PS-zeolite beads were characterized by SEM, XRD, FT-IR, and TGA. The optimum condition to prepare PS-zeolite beads was 1.25 g of PS content and 2 g of zeolite A. The removal efficiencies of Sr and Cs ions by the PS-zeolite beads increased as the solution pH increases and nearly reached a plateau at pH 4. The PS-zeolite beads prepared in this study showed a remarkably high selectivity for Sr ion and Cs ion under the coexistence of ions such as $Na^+$, $K^+$, $Mg^{2+}$, and $Ca^{2+}$. Zeolite particles detached from the PS-zeolite beads were not observed on this experiments, and also the PS-zeolite beads maintained the morphological structure on a SEM image. The removal efficiencies of Sr ions and Cs ions by PS-zeolite beads were maintained over 90% even after five adsorption-desorption cycles. These results implied that the prepared PS-zeolite beads could be an available adsorbent for the adsorption of Sr and Cs ions. These results suggest that the PS-zeolite can potentially be used as an adsorbent in radioactive ions removal for the treatment of industrial wastewater.

Preparation of Sulfonated PolySEBS/PS Blending Films (술폰화된 PolySEBS/PS Blending 필름의 제조)

  • Jang, Suk-Yong;Han, Sien-Ho
    • Applied Chemistry for Engineering
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    • v.19 no.2
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    • pp.205-208
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    • 2008
  • Sulfonated polySEBS and sulfonated PS were synthesized by sulfonation at the aromatic group of polySEBS and PS. Novel sulfonated polySEBS/sulfonated PS blending films for the ion exchange membrane of polymer electrolyte fuel cell were prepared from these sulfonated polymers. The proton conductivities of these blending films were varied in $10^{-2}{\sim}10^{-3}S/cm$ with the blending ratio of sulfonated polySEBS/sulfonated PS. Especially, the film prepared from the addition of the sulfonated PS (0.5 g) in the sulfonated polySEBS (10.0 g) has the best proton conductivity (0.07 S/cm) with ion exchange capacity (0.75 meq/g) and water uptake (25%).

Synthesis of Multi-Walled Carbon Nanotube/Polystyrene (MWCNT/PS) Composites by Solution Process and Their Thermal Behavior (용액공정을 이용한 다중벽 탄소 나노튜브/폴리스티렌(MWCNT/PS) 복합체 합성 및 열적 거동)

  • Teng, Dayong;Shin, Young Hwan;Kwon, Younghwan
    • Applied Chemistry for Engineering
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    • v.19 no.4
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    • pp.421-426
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    • 2008
  • Multi-walled carbon nanotube/polystyrene (MWCNT/PS) composites with various MWCNT contents were prepared by using a solution process with an aid of surfactant. Particularly, PS's with 3 different molecular weights (${\bar{M}}_n$ = 101500 g/mole for PS-1, ${\bar{M}}_n$ = 89900 g/mole for PS-2, and ${\bar{M}}_n$ = 85000 g/mole for PS-3) were used in this study. Thermal behavior of these composites was examined by using an oscillator rheometer at $210^{\circ}C$ and $180^{\circ}C$, of above and below the critical flow temperature ($T_{cf}{\sim}195^{\circ}C$) of PS matrix, respectively. The storage and loss modulus, and the complex viscosity of these composites increased with increasing MWCNT content at both temperatures. Largest increases in the frequency-dependent moduli and complex viscosity were observed between 2 wt% and 5 wt% of MWCNTs at $210^{\circ}C$ and $180^{\circ}C$. Only the composite at $210^{\circ}C$ showed the rheological phase transition from a viscous-dominant to an elastic-dominant behavior of the composites at a certain MWCNT content. The MWCNT content at the rheological phase transition of MWCNT/PS composites generally increased with decreasing molecular weight of PS, and was measured to be 3.5 wt% for MWCNT/PS-1, 3.2 wt% for MWCNT/PS-2, and 3.0 wt% for MWCNT/PS-3 composites.

Effect of PS-K on nile tilapia, Oreochromis niloticus, as an adjuvant and prophylactic agent (나일 틸라피아에 대한 질병예방제 및 면역보조제로서의 PS-K 효과 분석)

  • Park, Kyun-Hyun;Ha, Jai-Yi;Huh, Min-Do;Huh, Sung-Hoi;Jeong, Hyun-Do
    • Journal of fish pathology
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    • v.10 no.1
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    • pp.45-52
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    • 1997
  • A protein-bound polysaccharide preparation, PS-K, isolated from Coriolus versicolor was evaluated for its ability to enhance resistance against Edwardsiella tarda, causal agent of classical edwardsiellosis in tilapia, Oreochromis niloticus. Fish treated with a dose rate of 0.1 mg PS-K $g^{-1}$ body weight were challenged by intraperitoneal injection of E. tarda with different concentrations. Against low burdens of injections($1.2{\times}10^7$ and $1.2{\times}10^8$ cfu/fish), PS-K treated fish did not show any mortality compared to 50% and 100% mortality of control groups, respectively. However there was no increase of resistance against challenge with high concentrations of E. tarda ($1.2{\times}10^9$ and $1.2{\times}10^{10}$cfu /fish) except few days delaying of death. Tilapia injected with PS-K one day after intraperitoneal inoculation of E tarda($1.2{\times}10^7$ cfu/fish) showed 100% survival rate compared to control group of 50% survival rate. The result indicates the potential of PS-K as a prophylactic agent in aquaculture. The increased antibody response in fish received PS-K one week prior to FKC administration suggested the influence of PS-K on the specific humoral immunity and increased resistance against bacterial infection.

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Artificial diminution of the residual pesticides on horticultural crops using photosensitizers (감광제에 의한 원예작물중 잔류농약의 인위적 경감)

  • Lee, Jae-Koo;Kwon, Jeong-Wook;Ahn, Ki-Chang;Park, Ju-Hyoung;Lee, Jun-Su;Park, Jung-Ok
    • The Korean Journal of Pesticide Science
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    • v.3 no.2
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    • pp.47-53
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    • 1999
  • Photosensitizing activities of some photosensitizers (PS) for the artificial diminution of pesticide residues on horticultural crops were investigated. Five fungicides, iprodione, bitertanol, chlorothalonil, myclobutanil, and dichlofluanid were sprayed on apple and cucumber, followed by the application of each selected photosensitizer, and the samples were collected 0, 1, 3, 7, 15 days after the photosensitizer application and analyzed for the residual amounts. Of the 40 photosensitizers tested, six selected on the basis of the eliminating effect of pesticide residues were PS-1 (aromatic ketone), PS-2 (aromatic amine), PS-3 (quinone), PS-4 (inorganic compound), PS-5 (organic acid salt), and PS-6 (semiconductor photocatalyst). The residual amount of iprodione after 15 days of the application of PS-1 was 74% of that of the control. For bitertanol, the residual amount after 15 days of the application of PS-1 accounted for 78% of that of the control. The residual amounts of chlorothalonil after 1 day of the application of PS-1 and PS-2 accounted for 56 and 54% of those of the control, respectively. The residual amounts of iprodione on cucumber after 3 days of the application of the photosensitizers PS-1 and PS-2 were 44 and 67% of those of the untreated control, respectively. For myclobutanil, the residual amount after 15 days of the application of PS-6 accounted for 45% of that of the control. In case of dichlofluanid, the residual amount after 3 days of the application of PS-1 accounted for 44% of that of the control. Based on the results, PS-1 turned out to be the most promising photosensitizer for the accelerated photodegradation of the above fungicides on apple and cucumber.

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Diminution of Pesticide Residues on Crops and Soil by Accelerated Photolysis (광분해(光分解) 촉진(促進)에 의한 작물(作物) 및 토양중(土壤中) 농약잔유물(農藥殘留物)의 경감(輕減)에 관한 연구(硏究))

  • Lee, Jae-Koo;Jung, In-Sang;Kwon, Jeong-Wook;Ahn, Ki-Chang
    • Korean Journal of Environmental Agriculture
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    • v.17 no.2
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    • pp.107-116
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    • 1998
  • In an effort to reduce artifically the residual pesticides on crop and soil by accelerated photolysis,some 40 among the naturally occurring and synthetic coumpound were screened for photosensitization and/or photocatalysis and six promising chemicals were selected.The fungicides and the four selected photosensitizers and/or photocatalyst (PS) were applied to each crop.The results obtained are summarized as follows. 1. The wavelengths of maximum absortion (${\lambda}$max) and the molar absorptivites (${\varepsilon}$max) of procymidone,vinclozolin,and carbendazim in acentone were all 209 nm and 853,854,and 8740 respectively. 2. Of the 40 naturally-occuring and synthetic compounds screened,six promising ones were selected and designated as PS-1 (aromatic ketone),PS-2(aromatic amine)PS-3(quinone) ,PS-4 (inorganic compound),PS-5(organic acid salt) and PS-6(semiconductor photocatalyst). 3. In the accelerated photolysis of pesticide in soil by applying PS ,procymidone was decoposed rapidly by virtue of PS-2,being 59% of the control 3 days after application. 4. The vinclozolin residue in soil was reduced to 71% and 21% of the control 1 and 15 days,respectively,after PS-2 application. 5. The photolysis of carbendazim in soil was not accelerated by any of the PS tested. 6. The pesticide residues on the crop were prominently reduced by PS application.The procymidone residue on tomato was reduced to 47% of the control 15 days after PS-1 application and that on red pepper reduced to 57% 15 days after PS-2 application. 7. Vincrozolin residus remaining on tomato 1 and 15 days after PS-2 application were 38% and 56% of the control whereas those on the red pepper were 82% and 64%,respectively. 8. PS-2 was the most effective for the accelerated photolysis of carbendazim residues remaining on tomato, whereas on red pepper, the four of PS tested were all effective, but did not make much difference between them. This might be due to the shielding of sunlight by the leaves of red pepper not to exert the photosensitizing effect of PS-2 to the full.

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Isolation and Immunomodulating Activity of an Extracellular Polysaccharide Produced by Bacillus sp. PS-12 (Bacillus sp. PS-12가 생산하는 extracellular polysaccharide의 분리 및 immunomodulating activity)

  • Na, Ye-Seul;Suh, Hyun-Hyo
    • Journal of Life Science
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    • v.19 no.6
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    • pp.744-750
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    • 2009
  • A bacterial strain producing highly viscous extracellular polysaccharide was isolated from soil. Through morphological, physiological and chemotaxonomical studies, it was identified as a Bacillus sp. and named Bacillus sp. PS-12. The extracellular polysaccharide, named PS-12 was purified by ethanol precipitation, cetylpyridinium chloride (CPC) precipitation and gel permeation chromatography. The purified polysaccharide was found to consist of glucose, mannose, galactose, and fucose, with a molar ratio of approximately 7:3.2:2:1, respectively. PS-12 was investigated for its immunostimulating activity on murine macrophage RAW264.7 cells using an ELISA assay. PS-12 stimulated the production of TNF-${\alpha}$ to a level 50 times greater than the control and also induced 1L-6 secretion in a dose-dependent manner. The cytotoxicity on RAW264.7 cells by PS-12 was relatively low with 10% cytotoxicity at 2 ${\mu}g$/ml. These results indicate that PS-12 is less cytotoxic to immune cells and possess immunomodulating activity in which it can produce cytokines including TNF-${\alpha}$ and 1L-6 from macrophages.