• Korean Journal of Microbiology
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• v.30 no.2
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• pp.141-148
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• 1992

In order to overexpress bacteriophage PRD1 DNA polymerase in E. coli cells, the 2 kb HaeII fragment was isolated from phage genomic DNA. This fragment was then cloned into pEMBL/sup ex/ 3-expression vector. A specific 57bp deletion was performed by using uracil containing ss DNA and oligonucleotide spanning each region to remove an unwanted non-coding region. After this deletion, the PRD1 DNA polymerase gene is totally under the control of the vector promoter and SD sequence. Upon heat induction, a protein with an apparent size of 68 kdal was overexpressed as an active PRD1 DNA polymerase. The expression of PRD1 DNA polymerase was about 1% of total E. coli protein.

• Proceedings of the Korean Geotechical Society Conference
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• 1999.03a
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• pp.225-232
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• 1999

Construction case of PRD (Percussion Rotary Drill) pipe pile and matters to be attended in construction of PRD pile were reviewed. The compressive and uplifting static pile load tests for PRD piles were performed and, also, analysis by Pile Driving Analyzer was done. Based on these results, bearing components in each resisting part (that is: steel toe, external skin, and internal skin) were measured separately. The measured resisting force was compared to the value calculated by the estimated formula. The pile capacity was mobilized in steel toe area and the external skin friction and the internal friction were not produced. Thus, it could be considered that toe of PRD pile should be supported in hard bearing stratum (for example, the fresh soft rock).

• Korean Journal of Microbiology
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• v.29 no.4
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• pp.209-214
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• 1991

The PRD1 DNA polymerase is a small multi-functional enzyme containing conserved amino acid sequences shared by family B DNA polymerases. Thus the PRD1 DNA polymerase provides an useful model system with which to study structure-functional relationships of DNA polymerase molecules. In order to investigate the functional and structural roles of the highly conserved amino acid sequences, we have introduced three mutations into a conserved amino acid of the PRD1 DNA polymerase. Genetic complememtation study indicated that each mutation inactivated DNA polymerase catalytic activity.

• Journal of the Korean Institute of Gas
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• v.13 no.4
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• pp.33-39
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• 2009

This study is intended to experiment performance of pressure relief device and to extend the effective ways to prevent cylinders of NGV from bursting when they are exposed to local fire intensively or when they are overcharged under ambient temperature at fueling stations in summer. In the results of thermal cycling experiments, all products of three companies met the requirements for gas leakage in the qualification criteria between $82^{\circ}C$ and $-40^{\circ}C$. But the o-rings of two companies' specimens among the three companies' specimens got damaged under the accelerated conditions between $135^{\circ}C$ and $-45^{\circ}C$. It took one minute and thirty nine seconds for a glass bulb type of a thermal sensitive type PRD to activate and it took two minutes and thirty one seconds for a fusible plug type of a thermal sensitive type PRD to activate. These results indicated that a glass bulb type of a thermal sensitive type PRD was one minute faster than a fusible plug type of a thermal sensitive type PRD. Under the accelerated condition $135^{\circ}C$, the activation pressure of a pressure sensitive type PRD burst at 32.1 MPa and, under the condition of qualification criteria, it burst from 30.7 MPa to 32.1 MPa.. As a result of the experiment for performance of pressure relief device, in the case of the thermal sensitive type PRD, a glass bulb type is more effective to flame than a fusible plug type. we confirmed that the rupture pressure of a pressure sensitive type PRD could not be affected by temperature and pressure cycling.

• 기계와재료
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• v.26 no.1
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• pp.50-62
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• 2014

원전발전소에 납품되는 원전용 안전밸브의 성능은 ASME나 KEPIC와 같은 기술기준코드에 따라 설정압력, 블로우다운(blowdown), 풀리프트(Full lift), 누설(leakage), 방출용량(discharge flow rate)등과 같은 특성인자로 평가된다. 그 중에서도 방출용량은 ASME SEC III NB 7000 코드에 따라 ASME 용량인증이 요구되지만, 아직 국내에는 국제적으로 공인되니 압력방출장치 용량시험기관이 부재하여 외화유출 및 국내 PRD 기술개발에 많은 어려움이 있어왔으며, 이에 따라 원전 PRD 인증기관 개발을 통하여 세계적인 인프라 구축의 필요성이 부각되고 있다. PRD 인증기관 구축은 국내 원전 핵심기자재의 고급기술 국산화에 이바지 할 수 있고, PRD 성능 및 용량시험지원을 통하여 국내 밸브기술 경쟁력 확보하는 데 기여할 것으로 기대된다.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.18 no.7
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• pp.775-782
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• 2008

In this study, ground vibrations and aero space noises that is produced by the PRD(percussion rotary drill) were analyzed by work processes. Ground vibration equations were suggested by $2.798(SD)^{-0.793}$, $3.485(SD)^{-0.793}$, $3.705(SD)^{-0.793}$, according to experiment result, and these equations have reliability of 50%, 90%, 95%, respectively. Ground vibration values by the PRD method correspond to level of $5{\sim}34%$ of values that are assumed by the Attewell & Farmer's equation, and these result compares in reliability 50%. Also, those values were analyzed that correspond to level of $12{\sim}26%$ of ground vibration values by the Prof. Park etc.'s equations. But, the aero space noise was evaluated that is assumed by 88.9 dB(A) at separated distance 50m and is not satisfied even 85dB(A) that is the most negative noise value that present in domestic noise standard. The PRD method was analyzed that noise decrease effect exists hardly comparing with general pile driving method of construction. When is based in these results, the PRD method is judged that it is desirable that classify by the Low-vibration method more than the Non-violation noise method.

• Environmental Engineering Research
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• v.10 no.5
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• pp.239-246
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• 2005

The effect of Pt addition over $V_2O_5-WO_3/TiO_2$ catalyst supported on PRO-66 was investigated for NO reduction in order to develop the catalytic filter working at low temperature. Catalytic filters, $Pt-V_2O_5-WO_3/TiO_2/PRD$, were prepared by co-impregnation of Pt, V, and W precursors on $TiO_2$-coated ceramic filter named PRD (PRD-66). Titania was coated onto the pore surface of the ceramic filter using a vacuum aided-dip coating method. The Pt-loaded catalytic filter shifted the optimum working temperature from $260-320^{\circ}C$(for the catalytic filter without Pt addition) to $190-240^{\circ}C$, reducing 700 ppm NO to achieve the $N_x$ slip concentration($N_x\;=\;NO+N_2O+NO_2+NH_3$) less than 20 ppm at the face velocity of 2 cm/s. $Pt-V_2O_5-WO_3/TiO_2$ supported on PRD showed the similar catalytic activity for NO reduction with that supported on SiC filter as reported in a previous study, which implies the ceramic filter itself has no considerable interaction for the catalytic activity.

• Proceedings of the Korean Geotechical Society Conference
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• 2001.03a
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• pp.307-314
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• 2001

The bearing capacity of drilled shafts that take excavation by Percussion Rrotary Drilling(PRD) into consideration was evaluated using static and dynamic pile load tests. The emphasis was on quantifying the allowable bearing capacity and point load-transfer at the pile tip on seven instrumented steel piles. Of the seven instrumented piles, five piles are placed to the bottom of the excavation by rotary and pushing into the final depth of the excavation, as opposed to the two driven piles. Based on the results obtained, it is shown that the skin friction mobilized by PRD is much greater than point resistance, whereas in driven piles, the point resistance is greater than skin friction. It is also found that much greater pile capacity was proved in the case of drilled shafts, compared to the driven piles and thus, the excavation by rotary drilling gives reliable pile capacity required to design axially loaded piles.

• Journal of the Korean GEO-environmental Society
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• v.8 no.6
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• pp.5-12
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• 2007

Mudstone has characteristics that it has high enough strength and stiffness in a dry condition, but the strength and stiffness decrease in a wet condition with groundwater infiltration. The sliding of cut slope frequently encountered in Pohang area has been reported due to the rapid reduction of shear strength in mudstone after being exposed to the air. The study in this paper shows that mudstone having enough strength in a boring stage has lost the strength after installing PRD (percussion rotary drill) steel pipe pile inducing an insufficient bearing capacity. Field test has been performed to investigate the most favorable method for increasing a pile bearing capacity in mudstone with various methods such as MSG (Micro Silica Grouting) around the tip and side of a pile, the perimeter grouting combined with Micro pile reinforcement, and concrete filling after tip reinforcing grouting. MSG has been turned out to be the most favorable method for increasing a pile bearing capacity in mudstone, confirmed by the static load test.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.10
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• pp.1406-1415
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• 2013

The study was conducted to correlate the periparturient immune status in terms of neutrophil functions and cytokine expression in peripheral blood mononuclear cell culture with impending postpartum reproductive disorders in buffaloes. Forty pregnant buffaloes were observed for occurrence of postpartum reproductive disorders (PRD), i.e., metritis, endometritis and delayed uterine involution etc., during one week prepartum to four weeks postpartum period. A representative number (n = 6) of buffaloes that did not develop any PRD were included in group I (healthy, control), while the animals which experienced PRD were assigned into group II (PRD, n = 8). The blood samples were collected at weekly interval from one week prepartum to four weeks postpartum period considering the day of calving as 'd 0'. Differential leucocytes counts, superoxide and hydrogen peroxide production activity in isolated neutrophils and the mRNA expression profile of cytokines i.e., IL-2, IL-4 and IFN-${\gamma}$ in PBMC culture were studied in all the samples. A higher total leucocytes, neutrophil and band cells count along with impaired neutrophil functions i.e., lowered level of production of superoxide and hydrogen peroxide before parturition and during early postpartum period were observed in buffaloes developing PRD. Further, a lower expression of IL-2, IFN-${\gamma}$ and IL-4 mRNA in PBMC culture was observed at calving in buffaloes that subsequently developed PRD at later postpartum. Thus, suppression in neutrophil function and cytokine expression at prepartum to early postpartum period predisposes the buffaloes to develop postpartum reproductive disorders. Hence, monitoring of neutrophils function and cytokine expression profile would be effective to predict certain reproductive disorders at late pregnancy or immediately after parturition in buffaloes. In future, this may be a novel approach for determining suitable management and therapeutic decisions for prevention of commonly occurring reproductive disorders in farm animals.