• Korean Journal of Plant Resources
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• v.3 no.2
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• pp.123-128
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• 1990

In every reciprocal crosses of self pollination, interspecific pollination through ordinary stigma pollination of L. longiflorum and $L.{\;}{\times}{\;}elegans$, pollen vigorously germinated in stigma, and pollen tube was growing. But, 5 days after pollination, pollen tube stopping their growth in the same style as was observed in the cross of self pollination and interspecific pollination. Intrastylar pollination of $L.{\;}{\times}{\;}elegans$ and L. longiflorum passed stylar cannal through the basal part of styles. But, pollen tube that was growing to the ovule of the ovary was extremely small. In morphogical observation of ovary 10 days after pollination, it was observed, that every reciprocal crosses of L. longiflorum ${\times}$ L. ${\times}$ elegans was fertilized through intrastylar pollination, so that this technique was used to overcome cross incompatibilities.lities.

• Journal of Korean Society of Forest Science
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• v.98 no.6
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• pp.696-702
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• 2009

Yellow poplar (Liriodendron tulipifera L.) is an insect-pollinated tree species with large, perfect flower, and its seed sets average only about 10 percent naturally. In its controlled pollination, pollination bags are usually taken to prevent unwanted pollination, but bagging is an expensive and time-consuming process. Therefore, this study was conducted to determine the need of pollination bag by estimating how much unintended pollination would occur when different cross methods were applied. Five different pollination methods were applied as follows: 1) natural open pollination (i.e. insect pollination) as a reference, 2) self-pollination; no removing reproductive organs with bagging, 3) open pollination; emasculated(removing sepal, petal and stamen) without bagging, 4) controlled pollination; emasculated with bagging and 5) controlled pollination; emasculated without bagging. Very low value of full seed rate (0.2%) was observed in method 3, it was suggested that removing stamen and petal restrict the activity of pollen vectors like bee. Difference in the full seed rate between method 4 and method 5 was not significant (27.9% versus 24.0%, respectively). Consequently, controlled pollination without bagging might be an alternative method for extensive breeding and mass production of seeds in yellow poplar.

• International Journal of Industrial Entomology and Biomaterials
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• v.37 no.2
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• pp.109-116
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• 2018

We investigated the effects of honey bee (Apis mellifera L.) colony size on the pollination of greenhouse-cultivated watermelon grown under the forcing cultivation system. The highest pollination activity of bees was observed ($14.3{\pm}5.0$ honey bees/day) when the bee colony size was 10,000 followed by 7,500 and 5,000 honey bees. There was a positive correlation between the bee colony size and pollination activity (R = 0.262) but insignificant difference in fruit set with different honey bee colony sizes (88%-91%). Evaluation of physical properties revealed that the weight and shape of watermelon were also not significantly different among different colony sizes. However, larger the bee colony size, higher the number of seeds were fertilized and rate of seed fertilization (p > 0.05). Number of seeds and content of sugar were negatively correlated (R = -0.714). Fertilized seeds showed a significant increase in mealy flesh, which has a negative effect on fruit quality, compared with that of the unfertilized seeds. Overall, we found that a colony size of 5,000 honey bees was the most effective for the pollination of watermelon grown under forcing cultivation. A comparison of the effects of bee pollination with those of artificial pollination suggested that artificial pollination can be effectively replaced by bee pollination in the forcing cultivation of watermelon, because fruit set, weight, and shape by bee pollination were similar to those achieved by artificial pollination.

• Korean Journal of Organic Agriculture
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• v.24 no.4
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• pp.759-771
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• 2016

We compared the fruit set and the quality of the 'Niitaka' pear (Pyrus pyrifolia Nakai) among flowers pollinated by two bee species (Apis mellifera and Bombus terrestris) and pollinated artificial. The artificial pollination rate was 1.3 to 1.9 times higher than the bee pollination rate. Moreover, the artificially pollinated flowers produced fruit that was 5 to 10% higher in weight, 2 to 3% larger in size, and had a higher fruit shape index (L/D) than fruit pollinated by the bees. On economic analysis, net profit from insect pollinator was 93.5 to 97.1% of net profit from artificial pollination. Therefore, artificial pollination is more efficient than bee pollination in 'Niitaka' pear. However, regarding fruit quality and net profit, these results suggest that bee pollination can be an good alternative to artificial pollination in 'Niitaka' pear.

• Horticultural Science & Technology
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• v.19 no.3
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• pp.373-377
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• 2001

This study was conducted to find optimum pollination methods for overcoming pre-fertilization incompatibility in interspecific crosses between L. longiflorum 'Gelria' (LG) and L. cernuum (LC). In cross combination of $LC{\times}LG$, the survival rate of the ovaries was higher in the cut style pollinations than in normal stigmatic pollination. Among the various cut style pollination methods, pollination after cutting the styles 2 3mm length above the ovary and top of the ovary were the most effective ones. No ovaries and ovules survived in cross pollination of $LC{\times}LG$. In self-pollination of LG and cross pollination of $LC{\times}LG$, pollens germinated on the stigma by 12 hours and reached to the ovules by 90 hours after pollination. However, in the cross pollination of $LC{\times}LG$, pollen tubes elongated very slowly until 48 hours after pollination and a lot of deformed tubes were observed in the styler canal having swelled tube tip and irregular heavy callose deposition. The pollination time after anthesis also affected on the survival rate of ovaries and ovules showing the best result when pollinated on three days after anthesis. Stigma exudate promoted slightly the enlargement of ovaries, but the survival rate of ovaries and ovules was not increased.

• Korean Journal of Medicinal Crop Science
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• v.16 no.5
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• pp.287-293
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• 2008

This study was conducted to determine the characteristics of fertilization process and embryo development of Astragalus membranaceus Bunge (Astragali Radix) to provide basic data needed in its breeding. A. membranaceus showed poor seed setting when self-pollination was induced. When artificial pollination was induced, it showed less than 5% bearing in late August, but more than 13% bearing from the beginning of September 4th. The flower size was about $17.0\;mm{\times}4.0\;mm$ and pistils and stamens had the same length of 15.0mm at flowering stage. When self-pollination or cross-pollination was induced, pollen tubes extended to an ovule. While pollen tube was extending to the ovule, reproductive cell split and formed two male generative nuclei and a vegetative nucleus. In the case of self-pollination, fertilized embryo was not observed, but was formed in the case of cross-pollination. A. membranaceus is noted to have zygote self-incompatibility. In the case of cross-pollination, fertilization was observed in 6 to 8 h after pollination, where apical cell derivatives split after fertilization. A spherical pro-embryo was then formed three days after fertilization. The seed attained full shape with a seed coat showing its distinctive contour 15 days after fertilization. Thus, A. membranaceus in Leguminosae family is found to have zygote selfincompatibility although its flower shape is shown to match the self-compatibility plant.

• Journal of Ecology and Environment
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• v.45 no.4
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• pp.255-263
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• 2021

Background: Ecosystem service mapping is an important tool for decision-making in landscape planning and natural resource management. Today, pollination service mapping is based on the Lonsdorf model (InVEST software) that determines the availability of nesting and floral resources for each land cover and estimates pollination according to the foraging range of the desired species. However, it is argued that the Lonsdorf model has significant limitations in estimating pollination in a landscape that can affect the results of this model. Results: This paper presents a free software, named PollMap, that does not have the limitations of the Lonsdorf model. PollMap estimates the pollination service according to a modified version of the Lonsdorf model and assumes that only cells within the flight range of bees are important in the pollination mapping. This software is produced for estimating and mapping crop pollination in agricultural landscapes. The main assumption of this software is that in the agricultural landscapes, which are dominated by forest and agriculture ecosystems, forest patches serve only as a nesting habitat for wild bees and the surrounding fields provide floral resources. Conclusion: The present study provided new software for mapping crop pollination in agricultural landscapes that does not have the limitations of the Lonsdorf model. We showed that the use of the Lonsdorf model for pollination mapping requires attention to the limitations of this model, and by removing these limitations, we will need new software to obtain a reliable mapping of pollination in agricultural landscapes.

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.347-352
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• 2003

This work was undertaken to study the effect of stigmatic and cut-style pollination on self seed set in Lilium longiflorum and L. ${\times}$ formolongi, and their crosses as the female parent with other cultivars/genotypes. Stigmatic pollination of L. longiflorum cv. Gelria and Lorina resulted in cent per cent fruit set with mean number of seeds/fruit of 189 and 70, respectively. However, cut-style pollination resulted in 87％ and 40％ fruit set in Gelria and Lorina, respectively. The corresponding mean number of seeds/fruit was 53 and 20. In L. ${\times}$ formolongi, stigmatic pollination set 80％ fruits with 736 seeds/fruit. On the other hand, cut-style pollination resulted in 47％ fruit set with 81 seeds/fruit. The intraspecific stigmatic pollination of L. longiflorum cv. Gelria and Lorina with other cultivars formed 60％ fruits with a mean number of 18 seeds/fruit. However, only 20％ fruit set with mean number of seeds/fruit of 7 was recorded when cut-style pollination of L. longiflorum cultivar were pollinated with other cultivars/genotypes. In the intraspecific pollination of L. ${\times}$ formolongi cv. Raizan with Novia, fruit set resulting from stigmatic and cut-style pollination was 75％ and 50％, respectively with the corresponding mean number of seeds/fruit of 579 and 98. It was concluded that self as well as intraspecific seed set in the two species of Lilium gets considerably reduced as a result of cut-style pollination.

• The Plant Pathology Journal
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• v.37 no.3
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• pp.299-306
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• 2021

Pollination is an essential process for plants to carry on their generation. Pollination is carried out in various ways depending on the type of plant species. Among them, pollination by insect pollinator accounts for the most common. However, these pollinators have be decreasing in population density due to environmental factors. Therefore, use of artificial pollination is increasing. However, there is a lack of information on microorganisms present in the artificial pollens. We showed the composition of bacteria structure present in the artificial pollens of apple, kiwifruit, peach and pear, and contamination of high-risk pathogens was investigated. Acidovorax spp., Pantoea spp., Erwinia spp., Pseudomonas spp., and Xanthomonas spp., which are classified as potential high-risk pathogens, have been identified in imported pollens. This study presented the pollen-associated bacterial community structure, and the results are expected to be foundation for strengthening biosecurity in orchard industry.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.23 no.6
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• pp.29-41
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• 2020

Wild pollinators provide important pollination services for crops. However, their geographical ranges and impact on pollination services have not been fully explored within the scope of Korean agricultural land. This study aims to identify spatial supply-demand mismatches across orchard fields in the context of assessing pollination service risk. We first used National Ecosystem Survey data and a species distribution model (MaxEnt) to develop the geographic range of each of 32 wild pollinators belonging to three families (Diptera, Hymenoptera, and Lepidoptera). We then summed the modeled presence probability of each species to obtain a measure of spatially explicit pollinator richness. This modeled richness, defined as pollination supply, was compared with the summed area of orchard fields at the municipal boundary level to identify areas with supply-demand mismatches. The study found that Lepidoptera showed the highest species richness (8.3±1.5), followed by Hymenoptera (4.3±0.8) and Diptera (3.5±0.8) species. Median orchard area was 1.5 ㎢ (range of 0-176.7 ㎢) among 250 municipal regions in South Korea. The municipal regions were divided into three categories (tertiles) of low, middle, and high pollination supply and demand according to, respectivley, average polliator richness and orhard area. Finally, we found that 55 municipal regions (accounting for 49% of national orchard land) potentially faced high risk of pollination deficits, 81 regions (48% of national orchard land) faced intermediate risk, and 63 regions faced low risk (3% of national orchard land). In conclusion, this study revealed significant mismatch between pollination supply and demand and developed risk assessment map will guide our future efforts on pollinator habitat conservation and monitoring to conserve crop pollination services.