• Journal of Plant Biology
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• 제39권3호
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• pp.197-202
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• 1996

A pollen-specific cDNA clone, LMP50, was isolated from the mature pollen cDNA library of the Easter lily. The LMP50 transcript was highly abundant in mture pollen grains but not detectable in other organs. The LMP50 cDNA clone contains 1383 nucleotides and two open reading frames. The first codes for a peptide of 15 amino acid residues. The role of this peptide is nuclear. The second encodes a protein containing 329 amino acid residues. This protein exhibited a significant homology to human tartrate-resistant acid phosphatase and porcine uteroferrin. Both of these enzymes have been suggested to play a role in iron transport. Therefore, LMP50 may act as an iron carrier protein in mature pollen grains.

• 생약학회지
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• 제15권3호
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• pp.147-149
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• 1984

To examine pollen preparations which are being sold as a health food, one of these pollen tablets and one of the capsules were selected and investigated by microscopy. It was shown that the pollen coat which can act as a potent allergen was not removed and that the tablets contained other impurities. The results suggest that they are not safe as a food and that they may cause systemic allergy to certain persons. On the contrary, the pollens of the capsule preparation did not have the pollen coat and, therefore, have a less risk of causing allergy.

• Journal of Plant Biology
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• 제10권3_4호
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• pp.1-3
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• 1967

Observations were made on Crinum and Catharanthus pollen growth in artificial media by an ultraviolet transmission fluorescence microscope showing synergistic effect on pollen growth with calcium (Ca) and aureomycin. Bright yellow fluorescence of aureomycin enabling to trace out at tissue or cellular level did reveal that the greater accumulation of fluorescence occurred in the pollen tube wall if Ca was supplemented to the media than when aureomycin alone was present. The promotive pollen growth the media of Ca alone was further enhanced by the addtion of aureomycin. It was assumed that the promoted pollen growth with aureomycin in the Ca media was probably brought about by a supporting role of aureomycin in the Ca action.

• 식물조직배양학회지
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• 제27권5호
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• pp.411-413
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• 2000

The ability of microspores or young pollen grains (male gametophytes) to undergo developmetal switch to embryogenic (sporophytic) pathway exemplifies the concept of totipotency as applied to haploid posmeiotic cells. As a first step pollen is devoid of positional information provided in situ by the intact anther - by isolation and cultivation in vitro in artificial media. This is inevitably accompanied by some degree of stress response in microspore/pollen. It has been shown in both monocots and dicots that intentional stress treatment (mostly starvation or heat shock) greatly stimulates embryo induction rate. Using transgenic sHSP antisense Nicotiana tabacum we show that expression of small heat shock proteins is an integral part of successful embryo and later haploid plant production from pollen grains. Our recently published data show that sHSP chaperone function is optimal in the absence of ATP.

• Applied Biological Chemistry
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• 제47권4호
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• pp.430-433
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• 2004

Lilium cv. Casablanca pollen grains stored at $-70^{\circ}C$ were grown in pollen germination medium with Agrobacterium tumefaciens LBA4404 cells harboring pBI121 for 18 hr at $27^{\circ}C$. Following this, cefotaxime (250 mg/L) was treated for 6 hr to eradicate the bacterial cells. Histochemical GUS analysis revealed that the transgenic pollen displayed deep blue color mostly from 12 hr after the co-cultivation. Presence of $200\;{\mu}M$ acetosyringone was determined not to be more effective for GUS transformation than its absence. GUS DNA integration in the transgenic pollen genomic DNA was clearly demonstrated by Southern blot analysis.

• 한국조리학회지
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• 제10권4호
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• pp.178-189
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• 2004

This study was performed to investigate the effects of pine pollen and horseradish, which were known as antimicrobial materials, on the fermentation of Kimchi. The physicochemical and microbial changes of Kimchi were investigated during period 30-days at $10^{\circ}C$. Even though Kimchi added pine pollen or horseradish powder were sustained the higher pH and lower acidity after 10 days, it delayed only 1∼2 more days to reach optimal value of Kimchi fermentation in experimental groups than control. The horseradish powder showed the antimicrobial effect of Kimchi at initial stage, but thereafter that effect of pine pollen or horseradish were not significant. Therefore the addition of pine pollen or horseradish powder to Kimchi is not sufficiently effective for extension of shelf-life of Kimchi.

• 한국산림과학회지
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• 제6권1호
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• pp.43-48
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• 1967

In the present experiment the amino acid composition in the pollen of Pinus taeda and Pinus rigida and methbolic changes of the composition occurring in germinating pollen were examined by the method of paper chromatography. The results obtained in this study are as follows. 1. The mature pollen of Pinus taeda and Pinus rigida contained 12.00%, 13.19% crude protein respectively and sixteen protein-constituting amino acids and three unknowns were detected on the chromatogram. 2. Main components of the protein-constituting amino acids were leucines, valine, alanine, glutamic acid and aspartic acid. 3. The free amino acids such as leucines, valine, tyrosine, alanine and glutamic acid increased evidently during germination of the pine pollen.

• 한국지형학회지
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• 제23권2호
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• pp.1-13
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• 2016

This study analyzed pollen compositions in archaeological site on alluvial plain of Taehwa River, Taehwa-dong, Ulsan-si vegetational and environmental change during 6,200~3,000 yr BP. The results consist upward of Pollen Zone I (Inner Bay environment), Pollen Zone II (Quercus-Alnus stage) and Pollen Zone III (Alnus stage). The pollen grains of Fagopyrum and reddish gray silty horizon including artifacts of the Bronze Age suggest that there was cultivation on dry-field around the study site, although it is not sure that there was paddy-field cultivation.

• Current Optics and Photonics
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• 제2권5호
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• pp.460-467
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• 2018

Three-dimensional (3D) refractive-index (RI) imaging and quantitative analyses of angiosperm pollen grains are presented. Using optical diffraction tomography, the 3D RI structures of individual angiosperm pollen grains were measured without using labeling or other preparation techniques. Various physical quantities including volume, surface area, exine volume, and sphericity were determined from the measured RI tomograms of pollen grains. Exine skeletons, the distinct internal structures of angiosperm pollen grains, were identified and systematically analyzed.

• Clinical and Experimental Pediatrics
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• 제63권12호
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• pp.463-468
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• 2020

Pollen-food allergy syndrome (PFAS) is an immunoglobulin E-mediated immediate allergic reaction caused by cross-reactivity between pollen and the antigens of foods-such as fruits, vegetables, or nuts-in patients with pollen allergy. A 42.7% prevalence of PFAS in Korean pediatric patients with pollinosis was recently reported. PFAS is often called oral allergy syndrome because of mild symptoms such as itching, urticaria, and edema mainly in the lips, mouth, and pharynx that appear after food ingestion. However, reports of systemic reactions such as anaphylaxis have been increasing recently. This diversity in the degree of symptoms is related to the types of trigger foods and the characteristics of allergens, such as heat stability. When pediatric patients with pollen allergy are treated, attention should be paid to PFAS and an active effort should be made to diagnose it.