• Journal of Animal Science and Technology
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• 제66권4호
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• pp.781-791
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• 2024

Bovine viral diarrhea (BVD) is a single-stranded, positive-sense ribonucleic acid (RNA) virus belonging to the genus Pestivirus of the Flaviviridae family. BVD frequently causes economic losses to farmers. Among bovine viral diarrhea virus (BVDV) strains, BVDV-1b is predominant and widespread in Hanwoo calves. Reverse-transcription polymerase chain reaction (RT-PCR) is an essential method for diagnosing BVDV-1b and has become the gold standard for diagnosis in the Republic of Korea. However, this diagnostic method is time-consuming and requires expensive equipment. Therefore, Clustered regularly interspaced short palindromic repeats-Cas (CRISPR-Cas) systems have been used for point-of-care (POC) testing of viruses. Developing a sensitive and specific method for POC testing of BVDV-1b would be advantageous for controlling the spread of infection. Thus, this study aimed to develop a novel nucleic acid detection method using the CRISPR-Cas13 system for POC testing of BVDV-1b. The sequence of the BVD virus was extracted from National Center for Biotechnology Information (NC_001461.1), and the 5' untranslated region, commonly used for detection, was selected. CRISPR RNA (crRNA) was designed using the Cas13 design program and optimized for the expression and purification of the LwCas13a protein. Madin Darby bovine kidney (MDBK) cells were infected with BVDV-1b, incubated, and the viral RNA was extracted. To enable POC viral detection, the compatibility of the CRISPR-Cas13 system was verified with a paper-based strip through collateral cleavage activity. Finally, a colorimetric assay was used to evaluate the detection of BVDV-1b by combining the previously obtained crRNA and Cas13a protein on a paper strip. In conclusion, the CRISPR-Cas13 system is highly sensitive, specific, and capable of nucleic acid detection, making it an optimal system for the early point-of-care testing of BVDV-1b.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2007년도 추계학술대회 논문집
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• pp.293-294
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• 2007

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2014년도 춘계 종합학술대회 논문집
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• pp.265-266
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• 2014

• Journal of Veterinary Science
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• 제25권2호
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• pp.28.1-28.11
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• 2024

Background: Porcine circovirus type 2 (PCV2) infection is ubiquitous around the world. Diagnosis of the porcine circovirus-associated disease requires clinic-pathological elements together with the quantification of viral loads. Furthermore, given pig farms in regions lacking access to sufficient laboratory equipment, developing diagnostic devices with high accuracy, accessibility, and affordability is a necessity. Objectives: This study aims to investigate two newly developed diagnostic tools that may satisfy these criteria. Methods: We collected 250 specimens, including 170 PCV2-positive and 80 PCV2-negative samples. The standard diagnosis and cycle threshold (Ct) values were determined by quantitative polymerase chain reaction (qPCR). Then, two point-of-care (POC) diagnostic platforms, convective polymerase chain reaction (cPCR, qualitative assay: positive or negative results are shown) and EZtargex (quantitative assay: Ct values are shown), were examined and analyzed. Results: The sensitivity and specificity of cPCR were 88.23% and 100%, respectively; the sensitivity and specificity of EZtargex were 87.65% and 100%, respectively. These assays also showed excellent concordance compared with the qPCR assay (κ = 0.828 for cPCR and κ = 0.820 for EZtargex). The statistical analysis showed a great diagnostic power of the EZtargex assay to discriminate between samples with different levels of positivity. Conclusions: The two point-of-care diagnostic platforms are accurate, rapid, convenient and require little training for PCV2 diagnosis. These POC platforms can discriminate viral loads to predict the clinical status of the animals. The current study provided evidence that these diagnostics were applicable with high sensitivity and specificity in the diagnosis of PCV2 infection in the field.

• 한국콘텐츠학회논문지
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• 제11권4호
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• pp.312-320
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• 2011

이 논문에서는 일개 POC(Point Of Care) 시스템을 사용하는 의료기관을 중심으로 의약품 처방조제지원 시스템(Clinical Decision Support System, CDSS)과 조직성과와의 관계를 규명하는 것에 목적을 두고 있다. 이를 위하여 정보시스템 평가요소에 대해 정의를 내리고, CDSS의 성과 평가 모형을 제시하여 설문조사 분석을 통해 의약품 처방조제지원시스템의 도입 효과를 밝히고자 하였다. 분석결과 시스템 품질을 제외하고는 각 평가 영역들 사이에 인과성이 존재하는 것으로 분석되었으며, 통계적으로 유의하게 지지되는 것으로 분석되었다. 평가모형 검증결과 의약품처방최적화를 위한 CDSS의 시스템 품질이 사용자 만족도에 영향을 미친다는 근거를 발견할 수 없었다. 그러나 정보품질이 사용자의 만족도에 긍정적인 영향을 미치며 사용자 만족은 조직성과에 긍정적인 영향을 미치는 것으로 나타났다.

• Journal of Information Processing Systems
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• 제3권1호
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• pp.16-20
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• 2007

This paper describes the development of the sensor interface and driver program for a point of care (POC) device. The proposed pac device comprises an ARM9 embedded processor and eight-channel sensor input to measure various bio-signals. It features a user-friendly interface using a full-color TFT-LCD and touch-screen, and a bluetooth wireless communication module. The proposed device is based on the system on a programmable chip (SOPC). We use Altera's Excalibur device, which has an ARM9 and FPGA area on a chip, as a test bed for the development of interface hardware and driver software.

• Journal of Electrochemical Science and Technology
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• 제2권1호
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• pp.57-61
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• 2011

This paper describes an electrochemical immunoassay for simple, fast and quantitative detection of a urinary hippuric acid which is one of major biological indicator in toluene-exposed humans. The electrochemical system of immunoassay was based on the directly osmium complex conjugated with hippuric acid. With the competition between free hippuric acid (HA) and the osmium-hippuric acid conjugate (Os-HA) to bind with antibody hippuric acid (Anti-HA) coated onto gold nanoparticles, the electrical signals were proportional to urinary hippuric acid (HA) in the range of 0.01-5 mg/mL which is enough range to be used for in-field or point-of-care (POC) diagnosis. The proposed electrochemical method can be extended to the applications to detect a wide range of different small molecules in the field of health care.

• 한국산업정보학회논문지
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• 제15권2호
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• pp.115-126
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• 2010

현대사회는 정보화의 개념을 넘어서 유비쿼터스(Ubiquitous) 컴퓨팅 사회라고 말한다. 그리고 정보기술(IT)은 관련기술과의 융합으로 시스템들을 서로 통합하는 형태로 발전하고 있으며, 특히 유비쿼터스 환경에서의 의료정보 산업은 U-Healthcare 서비스 분야에 많은 관심을 가지고 있다. 본 연구에서는 첫째, U-Healthcare 서비스 환경과 통합의료정보시스템의 구성내용을 살펴보고, 둘째, 의료정보시스템의 통합을 위한 기본적 기술요소인 데이터웨어하우스, 네트워크, 통신 표준화 및 U-healthcare 서비스 관련기술 등을 검토한다. 마지막으로 이러한 기술적 요소들의 관점에서 U-Healthcare 서비스를 위한 새로운 통합의료정보시스템의 구축방안과 운영과제를 제안하였다. 이것은 고객들을 실시간으로 근접간호(POC : Point of Care)하고, 고객 개개인의 다양한 진료 데이터를 이용하여 정확한 진단을 하고, 그 정보가 다시 고객에게 전달됨으로써 고객만족이 향상될 것으로 기대된다.

• 한국가시화정보학회지
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• 제10권2호
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• pp.25-31
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• 2012

High-performance smartphones, equipped with a digital camera and an application software, can render conventional bench-top laboratory instruments mobile at affordable costs. As the smartphone-based devices are portable and wireless, they have wide applications especially in providing point-of-care (POC) tests in resource-constrained areas. We developed a hand-held diagnostic system, Mobile SmartScope, which consists of a small optical unit integrated with a smartphone. The performance of the SmartScope was favorably compared with that of conventional light microscopy in detecting and quantifying red blood cells. We also evaluated the fluorescence detection limit of the SmartScope incorporated with a blue light-emitting diode and appropriate optical filters by using fluorescently labeled microbeads for intensity calibration.

• Design & Manufacturing
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• 제6권2호
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• pp.74-78
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• 2012

A multi-cavity injection mold system of protein chip for point-of-care with cavity temperature and pressure sensors was proposed in this work. In advance of manufacturing for the multi-cavity injection mold system, a single cavity injection mold system to mold protein chip was considered. Injection molding analysis for the presented system was performed to optimize the process of the molding and suggest guides to design. On the basis of the results for the single cavity system, a multi-cavity injection mold system for protein chip was analyzed, designed and manufactured with cavity temperature and pressure sensors. Results of balanced filling for protein chip models were obtained from the presented mold system.