Journal of Electrochemical Science and Technology

The Korean Electrochemical Society (한국전기화학회)
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Electrochemical Method for Detecting Hippuric Acid Using Osmium-antigen Conjugate on the Gold Nanoparticles Modified Screen-printed Carbon Electrodes

  • Received : 2011.03.02
  • Accepted : 2011.03.24
  • Published : 2011.03.31
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Abstract

This paper describes an electrochemical immunoassay for simple, fast and quantitative detection of a urinary hippuric acid which is one of major biological indicator in toluene-exposed humans. The electrochemical system of immunoassay was based on the directly osmium complex conjugated with hippuric acid. With the competition between free hippuric acid (HA) and the osmium-hippuric acid conjugate (Os-HA) to bind with antibody hippuric acid (Anti-HA) coated onto gold nanoparticles, the electrical signals were proportional to urinary hippuric acid (HA) in the range of 0.01-5 mg/mL which is enough range to be used for in-field or point-of-care (POC) diagnosis. The proposed electrochemical method can be extended to the applications to detect a wide range of different small molecules in the field of health care.

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