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Standardization of Manufacturing Method of Young Radish Kimchi (Yulmoo Kimchi) and Young Radish Watery Kimchi (Yulmoo Mool-Kimchi) in Literatures (문헌에 나타난 열무김치 및 열무물김치 제조 방법의 표준화)

  • Kong, Chang-Suk;Kim, Do-Kyoung;Rhee, Sook-Hee;Rho, Chi-Woong;Hwang, Hae-Jun;Choi, Keyng-Lag;Park, Kun-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.1
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    • pp.126-130
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    • 2005
  • Manufacturing method and ingredient ratio for young radish kimchi (yulmoo kimchi) and young radish watery kimchi (yulmoo mool-kimchi) were standardized from literatures. Ingredients having frequency of use greater than 50% were only used in the standardization process. Green onion, red pepper, red pepper powder, garlic, ginger, and anchovy juice were included in young radish kimchi. Green pepper, red pepper, garlic, ginger, and starch were included in young radish watery kimchi. The standardized ingredients ratio of young radish kimchi (yulmoo kimchi) on young radish 100 g was as follows: green onion 8.0$\pm$3.8, crushed garlic 2.9$\pm$1.3, crushed ginger 1.6$\pm$0.7, red pepper 7.0$\pm$1.7, red pepper powder 4.2$\pm$1.2, and anchovy juice 3.7$\pm$0.5. The standardized ingredients ratio of young radish watery kimchi (yulmoo mool-kimchi) on added water 100 mL was as follows: young radish 50.6:$\pm$10.8, crushed garlic 3.0$\pm$0.7, crushed ginger $1.5\pm$0, green onion 3.3$\pm$1.3, green pepper 3.3$\pm$1.9, red pepper 2.4$\pm$1.3, and starch $1.5\pm$0.6.

The Effect of Bacillus-Fermented Scutellariae Radix Acupuncture Solution on Interleukin Production in Mouse Macrophage Stimulated by Lipopolysaccharide (바실러스균 발효황금약침액이 Lipopolysaccharide로 활성화된 마우스 대식세포의 인터루킨 생성에 미치는 영향)

  • Park, Wan-Su
    • Korean Journal of Acupuncture
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    • v.27 no.2
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    • pp.95-105
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    • 2010
  • Objectives : The purpose of this study is to investigate the effect of Bacillus-fermented Scutellariae Radix acupuncture solution (SB) on interleukin(IL) production in mouse macrophage stimulatedby lipopolysaccaride(LPS). Methods : Productions of interleukins were measured y High-throughput Multiplex Bead based Assay with Bio-plex Suspension Array System based on $xMAP^{(R)}$(multi-analyte profiling beads) technology. To begin with, cell culture supernatant was obtained after treatment with LPS(1 ${\mu}g/mL$) and SB for 24 hour. Then, it was incubated with the antibody-conj${\mu}g$ated beads for 30 minutes. And detection antibody was added and incubated for 30 minutes. After incubating for 30 minutes, Strepavidin-conjugated Phycoerythrin(SAPE) was then added. Incubating for another 30 minutes, the level of SAPE fluorescence was analyzed on Bio-plex Suspension Array System. Results : The results of the experiment are as follows. SB significantly inhibited the LPS-induced production of IL-3($9.15{\pm}0.35$ pg/mL) by $6.92{\pm}0.05,\;7.21{\pm}0.11,\;6.96{\pm}0.33,\;and\;7.45{\pm}0.74$ pg/mL at the concentration of 25, 50, 100, and 200 ${\mu}g/mL$ in mouse macrophage RAW 264.7 cells (p<0.05). SB significantly inhibited the LPS-induced production of IL-5($7.30{\pm}0.48$ pg/mL) by $6.50{\pm}0.29,\;6.30{\pm}0.25,\;6.30{\pm}0.25,\;and\;5.80{\pm}0.25$ pg/mL at the concentration of 25, 50 100, and 200 ${\mg}g/mL$ in RAW 264.7 cells (p<0.05). SB significantly inhibited the LPS-induced productiion of IL-9($17.26{\pm}0.19$ pg/mL) by $15.01{\pm}0.43$ pg/mL at the concentration of 25 ${\mu}g/mL$ in RAW 264.7 cells(p<0.05). SB significantly inhibited the LPS-induced productioh of IL-13($187.80{\pm}2.90$ pg/mL) by $152.80{\pm}4.25,\;172.80{\pm}3.97,\;162.10{\pm}6.67,\;and\;165.30{\pm}11.80$ pg/mL at the concentration fo 25, 50, 100, and 200 ${\mu}g/mL$ in RAW 264.7 cells(p<0.05). SB significantly inhibited the LPS-induced production of IL-17($18.30{\pm}0.95$ pg/mL) by $13.30{\pm}1.25,\;13.80{\pm}1.11,\;13.30{\pm}0.75,\;and\;14.00{\pm}1.08$ pg/mL at the concentration of 25, 50 100, and 200 ${\mu}g/mL$ in RAW 264.7 cells(p<0.05). SB significantly inhibited the LPS-induced production of IL-23($43.90{\pm}0.83$ pg/mL by $39.50{\pm}1.26,\;38.00{\pm}1.78,\;and\;39.60{\pm}2.49$ pg/mL at the concentration of 25, 100, and 200 ${\mu}g/mL$ in RAW 264.7 cells(p<0.05). Conclusions : These results suggest that SB has anti-inflammatory activity related with its inhibition of IL-3, IL-5, IL-13, IL-17, and IL-23 production in macrophages.

Expression and Characterization of the Human Lactoferrin in the Milk of Transgenic Mice

  • Z. Y. Zheng;Y. M. Han;Lee, K. K.
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.85-85
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    • 2003
  • Human lactoferrin (hLF) is an 80 kDa iron-binding glycoprotein that is expressed in high concentration in milk and in lesser amount in the secondary or specific granules of neutrophils and in plasma, LF is classically considered to be related to the binding, transport, and storage of iron. The transgenic mice carrying the human hLF gene in conjunction with the bovine $\beta$-casein promoter produced the human hLF in their milk during lactation. To screen transgenic mice, PCR was carried out using chromosomal DNA extracted from tail or toe tissues. In this study, stability of germ line transmission and expression of hLF were monitored up to generation Fl7 of a transgenic line. When female mouse of generation F9 was crossbred with normal male, generation F9 to Fl7 mice showed similar transmission rates ($66.0 \pm 12.57%, 42.0 \pm 14.98%, 72.2 \pm 25.45%, 50.0 \pm 16.70%, 65.7 \pm 6.45%, 48.6 \pm 14.65%, 54 1 \pm 18 11%, 57.8 \pm 16.16% and 48.6 \pm 20.66$, respectively), implying that the hLF gene can be transmitted stably up to long term generation in the transgenic mice For ELISA analysis, hLF expression levels were determined with an hLF ELISA kit in accordance with the supplier's protocol. Expression levels of human hLF from milk of generation F9 to Fl3 mice were $ 3.2 \pm 0.69 mg/ml, 3.1 \pm 0.81 mg/ml, 4.6 \pm 1.38 mg/ml, 3.1 \pm 0.42 mg/ml, and 4.5 \pm 1,48 mg/ml$, respectively. These expression levels were lower than that of founder (6.6 mg/$m\ell$) mouse. We concluded that transgenic mice faithfully passed the transgene on their progeny and successively secreted target proteins into their milk through several generations.

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Studies on electrocardiogram of the normal Korean native cattle II. Wave forms and amplitudes of the unipolar precordial chest leads (정상적(正常的)인 한우(韓牛)의 심전도(心電圖)에 관(關)한 연구(硏究) II. 흉부단극유도(胸部單極誘導)의 파형(波形)과 전위(電位))

  • Choi, In-hyuk;Kim, Soo-young;Kim, Nam-soo;Suh, Doo-seok
    • Korean Journal of Veterinary Research
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    • v.33 no.4
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    • pp.735-746
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    • 1993
  • Electrocardiographic parameters of amplitude and the shape of waves on the unipolar precordial chest leads in the normal Korean native cattles have been measured with a 3 channel electrocardiograph built in a computed and analysis. The study was conducted on 98 heads of mean age of 17.6 months. The wave forms of P, T and QRS complex wave in all leads showed various types. The parameters of the amplitude in the wave types showed the most frequency in each lead that were analyzed as follow : 1. In P wave, amplitudes of positive type showed a frequency of 92.9% and 93.9% in leads $CV_6LU$ and $CV_6LL$ that were $83.8{\pm}31.0{\mu}V$ and $76.0{\pm}30.7{\mu}V$, and negative type showed a frequency of 97.9% in lead V 10 that were $-80.2{\pm}29.4{\mu}V$, respectively. But the plate type in leads $CV_6RU$ and $CV_6RL$ showed frequency of 48% and 58.3%, respectively. 2. Average amplitude of the QRS complex were in a range of $277.0{\pm}154.0{\mu}V$ to $648.2{\pm}146.2{\mu}V$(mean of $418.8{\pm}139.4{\mu}V$) in all leads that were manifested the Low-Voltage QRS complex(below 1 mV in unipolar precordial leads). Average amplitudes of each wave type in the QRS complex were $-250.0{\pm}139.8{\mu}V$ and $-399.2{\pm}226.8{\mu}V$ in the QS group types that showed a frequency of 50.0% and 82.5% in the leads $CV_6LU$ and $CV_6LL$, respectively. And average amplitudes of the R group types showed a frequency of 85.6%, 56.1% and 75.8% in the $CV_6RU$, $CV_6RL$ and $V_{10}$ that were $321.5{\pm}142.1{\mu}V$, $271.6{\pm}139.9{\mu}V$ and $552.4{\pm}132.7{\mu}V$, respectively. 3. In T waves, Amplitudes of the positive type showed a frequency of 60.2%, 46.9% and 83.7% in leads of $CV_6LL$, $CV_6RU$ and $CV_6RL$ that were respectively $184.7{\pm}93.7{\mu}V$ $103.7{\pm}64.43{\mu}V$ and $111.8{\pm}39.3{\mu}V$, the negative type showed a frequency of 62.2% and 93.7% in leads $CV_6LU$ and $V_{10}$ that were $142.2{\pm}82.1{\mu}V$ and $-280.3{\pm}107.2{\mu}V$, respectively. 4. Average amplitude of ST segment were $3.7{\pm}33.1{\mu}V$, $0.9{\pm}23.1{\mu}V$, $10.9{\pm}28.6{\mu}V$, $5.8{\pm}28.3{\mu}V$ and $-34.7{\pm}48.4{\mu}V$ in leads $CV_6LL$, $CV_6RU$, $CV_6RL$, $CV_6LU$ and $V_{10}$ respectively.

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The Dietary, Blood and Urinary Levels of Lead, Iron and Copper in Self Selected Dietary Rural People (일상식이를 섭취하는 일부 농촌 성인 남녀의 식이, 혈액 및 뇨중의 납, 철분, 구리 수준)

  • 승정자
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.6
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    • pp.716-723
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    • 1993
  • This study was carried out to estimate intake level of Pb, Fe, Cu in rural area of Korea(12 males and 18 females). Analyses for the nutritional status of the record, duplicated diet collections, 24-hour urine collections, and venous blood sampling before measuring of blood pressure. The mean age and blood pressure of the subjects were 45.8$\pm$11.1years and 117.5$\pm$22.2/80.8$\pm$15.6mmHe in males, and 41.9$\pm$11.0years, 110.0$\pm$ 11.9/73.9$\pm$8.5mmHg in females, respectively. In respect to both males and females, mean BMI were 22.0$\pm$2.3, 23.1$\pm$3.0, mean Rohrer index were 131.8$\pm$14.8, 142.4$\pm$20.2 and mean skinfold thickness were 12.4$\pm$5.9mm, 25.3$\pm$7.4mm, respectively. The daily mean intakes of Pb, Fe, and Cu estimated for 3 days were 277.2$\pm$111.2$\mu\textrm{g}$/day, 12.7$\mu\textrm{g}$ 7.6mg/day, and 3.0$\pm$1.4mg/day in males and 192.0$\pm$72.4$\mu\textrm{g}$/day, 13.3$\pm$5.8mg/day, and 3.7 $\pm$1.7mg/day in females, respectively. The mean concentrations of serum Pb, Fe, Cu were 11.0$\pm$2.0$\mu\textrm{g}$/dl, 131.1$\pm$42.6$\mu\textrm{g}$/dl, 120.3$\pm$25.7$\mu\textrm{g}$/dl in males and 9.0$\pm$1.2$\mu\textrm{g}$/dl, 112.2$\pm$ 35.9$\mu\textrm{g}$/dl, 117.3$\pm$17.9$\mu\textrm{g}$/dl in females, respectively. The mean levels of Hb and Hct were 15.1$\pm$1.1g/dl, 45.2$\pm$3.3% in males and 13.1$\pm$0.8g/dl, 39.8$\pm$2.6% in females, respectively. The 24-hour urinary excretions of Pb, Fe, Cu were 35.5$\pm$10.0$\mu\textrm{g}$/day, 0.16$\pm$0.12mg/day, 60.12$\pm$0.02$\mu\textrm{g}$/day in males, and 25.3$\pm$11.0$\mu\textrm{g}$/day, 0.24$\pm$0.20mg/day, 70.07$\pm$0.03$\mu\textrm{g}$/day in females, respectively. In conclusion, the Pb intake in self selected diet of this subjects was not in the level that antagonized to Fe and Cu metabolism seriously.

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Energy expenditure measurement of various physical activity and correlation analysis of body weight and energy expenditure in elementary school children (일부 초등학생의 대표적 신체활동의 에너지소비량 측정 및 에너지소비량과 체중과의 상관성 분석)

  • Kim, Jae-Hee;Son, Hee-Ryoung;Choi, Jung-Sook;Kim, Eun-Kyung
    • Journal of Nutrition and Health
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    • v.48 no.2
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    • pp.180-191
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    • 2015
  • Purpose: There is a lack of data on the energy cost of children's everyday activities, adult values are often used as surrogates. In addition, the influence of body weight on the energy cost of activity when expressed as metabolic equivalents (METs) has not been vigorously explored. Methods: In this study 20 elementary school students 9~12 years of age completed 18 various physical activities while energy expenditure was measured continuously using a portable telemetry gas exchange system ($K_4b^2$, Cosmed, Rome, Italy). Results: The average age was 10.4 years and the average height and weight was 145.1 cm and 43.6 kg, respectively. Oxygen consumption ($VO_2$), energy expenditure and METs at the time of resting of the subjects were 5.41 mL/kg/min, 1.44 kcal/kg/h, and 1.5 METs, respectively. METs values by 18 physical activities were as follows: Homework and reading books (1.6 METs), playing game with a mobile phone or video while sitting (1.6 METs), watching TV while sitting on a comfortable chair (1.7 METs), playing video game or mobile phone game while standing (1.9 METs), sweeping a room with a broom (2.7 METs) and playing a board game (2.8 METs) belong to light intensity physical activities. By contrary, speedy walking and running were 6.6 and 6.7 METs, respectively, which belong to high intensity physical activities over 6.0 METs. When the effect of body weight on physical activity energy expenditure was determined, $R^2$ values increased with 0.116 (playing a game at sitting), 0.176 (climbing up and down stairs), 0.246 (slow walking), and 0.455 (running), which showed that higher activity intensity increased explanation power of body weight on METs value. Conclusion: This study is important for direct evaluation of energy expenditure by physical activities of children, and it could be used directly for revising and complementing the existing activity classification table to fit for children.

Changes of Blood Chemical Values on Dogs Infected with Babesia gibsoni (Babesia gibsoni 감염견의 혈액화학치 변화)

  • 이근우;장인호;송재찬;이성준
    • Journal of Veterinary Clinics
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    • v.15 no.1
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    • pp.219-221
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    • 1998
  • This report was conducted to determine the changes of blood and serum chemical values on dogs infected with Babesia gibsoni. Blood and serum were obtained and analyzed with a blood chemical analyzer and calculated by Chauvent's statistical analysis. The mean values and SD were as follows, $AST 33{\pm} 23 IU/L, ALT 22{\pm}15 IU/L, BUN 18$\pm $8 mg/dl, creatinine 1.6{\pm}0.8 mg/dl, CPK 564{\pm}214 IU/L, LDH 208{\pm} 78 IU/L, ALP 52{\pm} 8 IU/L, glucose 89{\pm}38 mg/dl, RBC 3.04{\pm}0.77{\times}10^{6}/{\mu}l, hemoglobin 6.1{\pm} 1.3 g/dl, PCV 18.0{\pm }4.3%, $respectively.

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A comparative study of the puberty suppression effect of gonadotropin-releasing hormone agonist in precocious or early puberty girls (중추성 성조숙증 및 조기 사춘기 여아에서 성선자극호르몬 방출호르몬작용제의 용량에 따른 사춘기 억제 효과 비교)

  • Shim, Kye-Shik;Bae, Chong-Woo;Yang, You-Jung
    • Clinical and Experimental Pediatrics
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    • v.51 no.6
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    • pp.634-639
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    • 2008
  • Purpose : There has been considerable disagreement regarding the most appropriate dosage of gonadotropin-releasing hormone agonist in cases of central precocious puberty. The aim of this study was to determine the appropriate dosage for suppression of the puberty in girls with central precocious or early puberty. Methods : Twenty-two girls with early puberty were randomly subjected to 3 types of dosages of leuprolide acetate for at least 6 months. The number of cases in groups 1, 2, and 3 were 7, 7, and 8, and dosages were 70, 90, and $110{\mu}g/kg/-month$, respectively. Height, weight, bone age, Tanner stage of breast development, and serum levels of LH, FSH, estradiol, and progesterone were measured before treatment and after 6 months of treatment. The number of cases of puberty suppression was compared using a modified puberty suppression score with a nonparametric chi-square test. Results : There were no significant differences of chronologic and bone ages among the groups. There was a significant decrease in height SDS gain after 6 months in group 3 (P<0.05) compared with groups 1 and 2. Serum levels of LH, FSH, estradiol and progesterone were all significantly decreased after treatment in all 3 groups (P<0.05). The number of cases of puberty suppression in each group were 4 (57%), 5 (71%), and 8 (100%). There was a significantly increased proportion of suppression of puberty in group 3 (P<0.05). Conclusion : It was necessary to use a higher dose of gonadotropin-releasing hormone agonist to suppress early puberty in girls; however further longitudinal study will be needed for their prognosis of final adult height.

Development of continuous indoor rearing technique of mole cricket, Gryllotalpa orientalis Burmeister (Orthoptera: Gryllotalpidae) (땅강아지(Gryllotalpa orientalis Burmeister)의 실내 누대사육 기술개발)

  • Park, Young-Kyu;Lee, Young-Bo
    • Journal of Sericultural and Entomological Science
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    • v.52 no.2
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    • pp.96-101
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    • 2014
  • The present study was conducted for two years to develop the indoor breeding system of mole cricket, Gryllotalpa orientalis Burmeister. In 2013, the total mean number of progenies was $430.{\pm}20.0$ ($mean{\pm}SE$); $15.3{\pm}25.8$ of 2nd-3rd instars, $28.8{\pm}24.0$ 3rd-5th and $2.4{\pm}2.7$ 6th-8th. In 2014, the rearing yielded similar result to that of 2013; $43.5{\pm}31.0$ in total with $25.8{\pm}25.6$ of 2nd-3rd instars, $23.6{\pm}21.3$ 3rd-5th and $2.9{\pm}2.3$ 6th-8th. The successful breeding rate using indoor cages was 46.9% in 2013 and 50.0% in 2014. In total 147 individuals were successfully reared in an outdoor rearing cage with 25 females released and 78 individuals in an indoor rearing cage with 100 females and 50 males released. 86.4% and 89.7% of the nymphs reared were collected within 25cm depth of the soil in the outdoor and indoor cage, respectively.

Characteristics of Nutrient Uptake by Aquatic Plant in Constructed Wetlands for Treating Livestock Wastewater (가축폐수 처리 위한 인공습지에서 수생식물의 영양염류 흡수 특성)

  • Kim, Kyeong-Jin;Kim, Jeong-Seob;Kim, Lee-Hyung;Yang, Keum-Chul
    • Journal of Wetlands Research
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    • v.14 no.1
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    • pp.121-130
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    • 2012
  • This study is to investigate the removal rates of nutrient in water, the biomass of water plants, and the total amounts of T-N and T-P uptakes by water plants to evaluate the ecological characteristics of the constructed wetland for treatment of livestock wastewater in Yangji-ri, Nonsan-si from June through November 2011. During the experimental period, the monthly plant biomass of constructed wetland in July were the highest as 669.4 kg, while the lowest in November as 200.1 kg. The research showed that the average nitrogen and phosphorus contents in aboveground and underground biomass of Phragmites australis were $21.9{\pm}0.6{\sim}32.1{\pm}1.5mg/g$, $15.1{\pm}5.5{\sim}24.9{\pm}5.7mg/g$, $1.5{\pm}0.3{\sim}2.4{\pm}0.2mg/g$ and $1.6{\pm}0.6{\sim}2.5{\pm}0.6mg/g$, respectively. The maximum amount of T-N and T-P uptake by Phragmites australis were 28.0 kg in July and 2.5 kg in June, respectively, while the minimum amount of T-N and T-P uptake by Phragmites australis were 9.7 kg and 0.7 kg in November, respectively. The removal rates of T-N and T-P in constructed wetland for treating livestock wastewater were 23.0 % and 59.1 %, respectively. The results of this study is expected to deduce the circulation of contaminants and nutrient in the wetland afterwards.