• Title/Summary/Keyword: PKNU-3

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Transcriptional Response of Major Antioxidant Enzyme Genes to Heat Stress in Mud Loach (Misgurnus mizolepis) (고온 스트레스에 대한 미꾸라지(Misgurnus mizolepis) 항산화 효소 유전자들의 발현 특징)

  • Cho Young-Sun;Lee Sang-Yoon;Bang In-Chul;Kim Dong-Soo;Nam Yoon-Kwon
    • Journal of Aquaculture
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    • v.19 no.3
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    • pp.157-165
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    • 2006
  • Expression of major antioxidant enzyme (AOE) including Cu/Zn superoxide dismutase (Cu/Zn-SOD), catalase (CAT), glutathione-S-transferase (GST) and 3 glutathione peroxidase isotypes (GPXs) at mRNA levels during heat stress was examined in mud loach (Misgurnus mizolepis) liver. Based on the semi-quantitative RT-PCR, real-time RT-PCR and/or northern dot blot hybridization, the antioxidant enzyme genes were generally up-regulated during elevation of water temperature from $23^{\circ}C$ up to $32^{\circ}C$. GPXs and SOD displayed the most significant elevation of mRNA levels (up to 3 and 2 folds, respectively) while CAT showed the steady-state expression irrespective of thermal conditions. GST represented the relatively moderate response (1.3-fold increase) in its transcription to thermal stress. The transcriptional activation of AOE genes was not significant at the treatment temperature lower than $29^{\circ}C$. Increased mRNA levels of GPX (extracellular form) and SOD genes in the fish exposed to $32^{\circ}C$ was readily detectable 1 day after exposure to heat stress.

Effects of Carbon Content on the Weldability of B-Containing $620^{\circ}C$ Grade High Cr Ferritic Cast Steel for Turbine Casing (B 첨가 $620^{\circ}C$급 터빈 케이싱용 고Cr 페라이트계 주강의 용접성에 미치는 탄소함량의 영향)

  • Seo, Won-Chan;Bang, Kook-Soo;Chi, Byung-Ha
    • Journal of Ocean Engineering and Technology
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    • v.22 no.6
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    • pp.41-45
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    • 2008
  • Effectsof carbon content on the weldability of B-containing 620 grade high Cr ferritic cast steels were investigated. Cast steel with lower carbon content of 0.07% showed lower HAZ hardness because of the formation of lower carbon martensite in HAZ. It also showed less solidification cracking susceptibility in weld metal because of the formation of delta ferrite. However, hot ductility showed no difference between cast steels with lower and higher carbon contents. Cast steel with lower carbon content showed greater HAZ softening after PWHT in the region heated between AC1 and AC3 because of its higher base metal hardness.

A Comparison of the Direct Shear Test and Shear Simulation Based on the Discrete Element Method (직접전단시험과 이산요소법에 기반한 전단 시뮬레이션과의 비교)

  • Jung, Sung-Heon;Sohn, Jeong-Hyun
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.19 no.3
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    • pp.86-91
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    • 2020
  • An important factor of rough road modeling is analyzing the shear behavior properties of the rough road. These properties influence the drawbar pull of the tool when interacting with the soil used in agriculture. Furthermore, shear behavior properties are important because sinkage and shear stress are generated when wheels drive on rough roads. In this study, we performed a direct shear test to investigate the shear behavior properties of soils and compare with the direct shear simulation; shear force derived by the coupled analysis of discrete element method; and multi-body dynamics. Soil contact parameters were measured in a wheel and soil contact simulation followed by comparison of the simulated and experimentally measured shear force.

In vitro Antibacterial and Synergistic Activity of an Ecklonia cava Extract against Anti biotic-Resistant Streptococcus parauberis

  • Eom, Sung-Hwan;Santos, Jeniel A.;Kim, Ji-Hoon;Jung, Won-Kyo;Kim, Do-Hyung;Kim, Young-Mog
    • Fisheries and Aquatic Sciences
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    • v.18 no.3
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    • pp.241-247
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    • 2015
  • In an effort to discover alternative phytotherapeutic antimicrobial agents to combat Streptococcus parauberis, a fish pathogenic bacterium, we evaluated the antibacterial activity of seaweed extracts in vitro. A methanolic extract of Ecklonia cava exhibited strong antibacterial activity against S. parauberis isolated from olive flounder Paralichthys olivaceus. Furthermore, the n-hexane soluble (Hexane) fraction of the E. cava methanolic extract exhibited the greatest antibacterial effect on S. parauberis strains with a minimum inhibitory concentration (MIC) ranging from 256 to $1,024{\mu}g/mL$. In addition, the MIC values of oxytetracycline against antibiotic-resistant S. parauberis were markedly reduced up to 64-fold in combination with the Hexane fraction, suggesting that the antibacterial activity of the antibiotic was restored when combined with the Hexane fraction. The interaction between both antibiotics and the Hexane fraction was assessed by the fractional inhibitory concentration (FIC) index. The Hexane fraction and oxytetracycline combination against antibiotic-resistant S. parauberis strains resulted in a median ${\sum}FIC$ range of 0.502 to 0.516. Thus, the synergistic ranges of median ${\sum}FIC$ < 1 were observed for all combinations of the Hexane fraction and oxytetracycline against S. parauberis. To the best of our knowledge, this is the first report indicating the efficacy of an E. cava extract against fish pathogenic bacterium S. parauberis.

Enhanced reutilization value of shrimp-shell waste via fed-batch biodegradation with higher production of reducing sugar, antioxidant, and DNA protective compounds

  • Rashid, Harun Ar;Jung, Hyun Yi;Kim, Joong Kyun
    • Fisheries and Aquatic Sciences
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    • v.21 no.10
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    • pp.33.1-33.11
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    • 2018
  • As a process for commercial application, production of reducing sugar, antioxidant, and DNA protective compounds from shrimp-shell powder was investigated in a fed-batch biodegradation using Bacillus cereus EW5. The fed-batch biodegradation was operated in a 5-L bioreactor for 96 h according to three times pulse-feeding strategy. On the basis of the equal working volume (3 L), the fed-batch biodegradation showed a better production of the target compounds than the batch biodegradation, with higher cell density and shortened biodegradation period. The maximum values of the target compounds were 0.297 mg/mL of reducing sugar, 92.35% DPPH radical scavenging activity, 98.16% ABTS radical scavenging activity, and 1.55 reducing power at $A_{700}$, which were approximately 12.1, 3.4, 5.2, and 8.4% enhanced, respectively, compared with those obtained from the batch biodegradation. The fed-batch culture supernatant also showed the enhanced DNA damage inhibition activity than the batch culture supernatant. As a result, the fed-batch biodegradation accompanied by high cell density could produce more useful compounds, enabling an increase in the reutilization value of shrimp-shell waste.

Laser Welding of AZ31B-H24 Mg Alloy with AZ61 Filler Wire (AZ61 필러 와이어를 첨가한 AZ31B-H24 마그네슘 합금의 레이저 용접)

  • Ryu, Chung-Sun;Bang, Kook-Soo;Lee, Mok-Young;Chang, Woong-Sung
    • Journal of Welding and Joining
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    • v.26 no.6
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    • pp.54-58
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    • 2008
  • Laser welding with AZ61 filler wire was carried out to improve formability though reduction of porosity and formation of under fill bead. Optimum welding condition and mechanical properties of butt joint for $400{\times}500{\times}1.3mm$ magnesium sheets were studied. Optimal welding conditions of laser power, welding speed, and defocusing length are 1000W, 3m/min, and 2mm, respectively. Results of tensile test indicated that both tensile strength and elongation of specimens welded with filler wire were improved at room temperature because of reduction of porosity and under-filled bead formation in addition to the precipitation hardening and microstructure refinement by Al-Mn and Mg-Al-Zn precipitates. At elevated temperature of $200{\sim}350^{\circ}C$, fracture location of tensile specimen was shifted from weld metal to base metal, indicating less softening of weld metal than base metal.

Nanoscale Pattern Formation of Li2CO3 for Lithium-Ion Battery Anode Material by Pattern Transfer Printing (패턴전사 프린팅을 활용한 리튬이온 배터리 양극 기초소재 Li2CO3의 나노스케일 패턴화 방법)

  • Kang, Young Lim;Park, Tae Wan;Park, Eun-Soo;Lee, Junghoon;Wang, Jei-Pil;Park, Woon Ik
    • Journal of the Microelectronics and Packaging Society
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    • v.27 no.4
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    • pp.83-89
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    • 2020
  • For the past few decades, as part of efforts to protect the environment where fossil fuels, which have been a key energy resource for mankind, are becoming increasingly depleted and pollution due to industrial development, ecofriendly secondary batteries, hydrogen generating energy devices, energy storage systems, and many other new energy technologies are being developed. Among them, the lithium-ion battery (LIB) is considered to be a next-generation energy device suitable for application as a large-capacity battery and capable of industrial application due to its high energy density and long lifespan. However, considering the growing battery market such as eco-friendly electric vehicles and drones, it is expected that a large amount of battery waste will spill out from some point due to the end of life. In order to prepare for this situation, development of a process for recovering lithium and various valuable metals from waste batteries is required, and at the same time, a plan to recycle them is socially required. In this study, we introduce a nanoscale pattern transfer printing (NTP) process of Li2CO3, a representative anode material for lithium ion batteries, one of the strategic materials for recycling waste batteries. First, Li2CO3 powder was formed by pressing in a vacuum, and a 3-inch sputter target for very pure Li2CO3 thin film deposition was successfully produced through high-temperature sintering. The target was mounted on a sputtering device, and a well-ordered Li2CO3 line pattern with a width of 250 nm was successfully obtained on the Si substrate using the NTP process. In addition, based on the nTP method, the periodic Li2CO3 line patterns were formed on the surfaces of metal, glass, flexible polymer substrates, and even curved goggles. These results are expected to be applied to the thin films of various functional materials used in battery devices in the future, and is also expected to be particularly helpful in improving the performance of lithium-ion battery devices on various substrates.

A Study on Performance Evaluation based on Packet Dropping in ATM Network . New Scheme Proposal

  • Park, Seung-Seob;Yuk, Dong-Cheol
    • Journal of Navigation and Port Research
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    • v.27 no.3
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    • pp.283-288
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    • 2003
  • Recently, the growth of applications and services over high-speed Internet increases, ATM networks as wide area back-bone has been a major solution. As the conventional TCP/IP suite is still the standard protocol used to support upper application on current. Internet, the issues regarding whether TCP/IP will operate efficiently on top of an ATM infrastructure and how to control its QoS still remain for studies. TCP uses a window-based protocol for flow control in the transport layer. When TCP uses the UBR service in ATM layer, the control method is only buffer management. If a cell is discarded in ATM layer, one whole packet of TCP will be lost; this fact occur the most TCP performance degradation. Several dropping strategies, such as Tail Drop, EPD, PPD, SPD, FBA, have been proposed to improve the TCP performance over ATM. In this paper, to improve the TCP performance, we propose a packet dropping scheme that is based on comparison with EPD, SPD and FBA. Our proposed scheme is applied to schemes discussed in the previous technology. Our proposed scheme does not need to know each connection's mean packet size. When the buffer exceeds the given threshold, it is based on comparison between the number of dropped packet and the approved packet. Our results are reported and discussed for comparing these discarding schemes under similar conditions. Although the number of virtual channel (VC) is increased, the simulation results showed that the proposed scheme can allocate more fairly each VC than other scheme.

Real-time monitoring of ship's dynamic behavior characteristics by AIS (AIS에 의한 선박거동의 실시간 모니터링)

  • Lee, Dae-Jae
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.44 no.3
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    • pp.218-228
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    • 2008
  • This paper describes on the real-time tracking of ship's dynamic behavior by AIS information in the coastal waters. The AIS data was received at a land station by using the antenna of AIS receiver mounted on the rooftop of the laboratory, Pukyong National University (PKNU), Busan, Korea, and stored as a NMEA format of serial output sentence of VDM(VHF Data-Link Message) and displayed on the ENC(Electronic Navigational Chart) of a PC-based ECDIS. In this study, the AIS receiver was mainly used to obtain the dynamic information that is necessary to evaluate and track the movement situation of training ship "KAYA" of PKNU in the coastal waters. The change of position with time for the ship turning under the rudder angle of port $30^{\circ}$ was correctly tracked with the turning circle of 940 m in diameter on the ENC of a PC-based ECDIS. Then, the dynamic information of the AIS system was updated every 6.29 seconds under the turning situation for the speed of 10.9 knots and every 21.65 seconds under the situation running at the speed of 11.05 knots on the straight line route of $155^{\circ}$, respectively. In case of AIS target tracking in the inshore zone behind large topographical obstructions, such as mountain and apartment buildings, the update rate of dynamic information was irregularly changed by the existence of land obstacles. However, the position tracking by AIS information under the situation existing no sea obstructions was achieved in real or near real-time and the instant presentation of course alternations for the ship was correctly monitored by using a PC-based ECDIS. From these results, we concluded that the PC-based ECDIS technology and methodology combined with the AIS information can be easily extended and applied to the surveillance and management for the fishing operation of fishing vessels in the coastal zone and in the EEZ fishing grounds.

Detection of The Pine Trees Damaged by Pine Wilt Disease using High Resolution Satellite and Airborne Optical Imagery

  • Lee, Seung-Ho;Cho, Hyun-Kook;Lee, Woo-Kyun
    • Korean Journal of Remote Sensing
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    • v.23 no.5
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    • pp.409-420
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    • 2007
  • Since 1988, pine wilt disease has spread over rapidly in Korea. It is not easy to detect the damaged pine trees by pine wilt disease from conventional remote sensing skills. Thus, many possibilities were investigated to detect the damaged pines using various kinds of remote sensing data including high spatial resolution satellite image of 2000/2003 IKONOS and 2005 QuickBird, aerial photos, and digital airborne data, too. Time series of B&W aerial photos at the scale of 1:6,000 were used to validate the results. A local maximum filtering was adapted to determine whether the damaged pines could be detected or not at the tree level from high resolution satellite images, and to locate the damaged trees. Several enhancement methods such as NDVI and image transformations were examined to find out the optimal detection method. Considering the mean crown radius of pine trees, local maximum filter with 3 pixels in radius was adapted to detect the damaged trees on IKONOS image. CIR images of 50 cm resolution were taken by PKNU-3(REDLAKE MS4000) sensor. The simulated CIR images with resolutions of 1 m, 2 m, and 4 m were generated to test the possibility of tree detection both in a stereo and a single mode. In conclusion, in order to detect the pine tree damaged by pine wilt disease at a tree level from satellite image, a spatial resolution might be less than 1 m in a single mode and/or 1 m in a stereo mode.