• Title/Summary/Keyword: PK-15

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Effects of dietary energy sources on early postmortem muscle metabolism of finishing pigs

  • Li, Yanjiao;Yu, Changning;Li, Jiaolong;Zhang, Lin;Gao, Feng;Zhou, Guanghong
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.12
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    • pp.1764-1772
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    • 2017
  • Objective: This study investigated the effects of different dietary energy sources on early postmortem muscle metabolism of finishing pigs. Methods: Seventy-two barrow ($Duroc{\times}Landrace{\times}Yorkshire$, DLY) pigs ($65.0{\pm}2.0kg$) were allotted to three iso-energetic and iso-nitrogenous diets: A (44.1% starch, 5.9% crude fat, and 12.6% neutral detergent fibre [NDF]), B (37.6% starch, 9.5% crude fat, and 15.4% NDF) or C (30.9% starch, 14.3% crude fat, and 17.8% NDF). After the duration of 28-day feeding experiment, 24 pigs (eight per treatment) were slaughtered and the M. longissimus lumborum (LL) samples at 45 min postmortem were collected. Results: Compared with diet A, diet C resulted in greater adenosine triphosphate and decreased phosphocreatine (PCr) concentrations, greater activity of creatine kinase and reduced percentage bound activities of hexokinase (HK), and pyruvate kinase (PK) in LL muscles (p<0.05). Moreover, diet C decreased the phosphor-AKT level and increased the hydroxy-hypoxia-inducible $factor-1{\alpha}$ ($HIF-1{\alpha}$) level, as well as decreased the bound protein expressions of HK II, PKM2, and lactate dehydrogenase A (p<0.05). Conclusion: Diet C with the lowest level of starch and the highest levels of fat and NDF could enhance the PCr utilization and attenuate glycolysis early postmortem in LL muscle of finishing pigs.

Effect of Continuous use of Inorganic Fertilizer on the Soil Organisms and Food Chain (무기질비료의 장기연용이 토양생물 및 먹이연쇄에 미치는 영향)

  • Eo, Jinu;Park, Kee-Choon;Park, Jin-Myeon;Kim, Myung-Hyun;Choi, Soon-Kun;Bang, Hea-Seon
    • Korean Journal of Environmental Agriculture
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    • v.35 no.1
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    • pp.39-45
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    • 2016
  • BACKGROUND: This study aimed to evaluate the combined effects of three components (NPK) of chemical fertilizers with basal application of compost on soil organisms.METHODS AND RESULTS: The soil was treated with five treatments continuously for 15 years: control, PK, NK, NP and NPK. The application of N increased plant growth or biomass, and enhanced organic matter content in the soils. Levels of microbial phospholipid fatty acids (PLFAs) in the soils did not show marked differences among the soils treated with different treatments. However, the principal component analysis showed the changes in the structure of the microbial community in the soil, depending on treatments added. Nitrogen application caused a decrease of pH and an increase of EC in the soils, and these environmental stresses appeared to offset the promoting effect of increased organic matter content on microbial abundance. The abundance of bacterivorous nematodes was the highest in the soils after treating NPK; however, the abundance of fungivorous nematodes was unaffected. There was no significant correlation between the abundances of microbial groups and their feeders. Organic matter content was significantly correlated with the abundance of nematodes in the soils.CONCLUSION: Our results showed that chemical fertilizers affect the soil food chains through both biotic and abiotic factors, and a trophic cascade in the soils may not occur in response to long-term fertilization.

Growth Characters and Productivities of Corn Varieties at Deakwanryoung District of Korea (대관령지역에서 사료용 옥수수 품종별 생육특성과 수량성)

  • 임영철;정종원;한성윤;최기준;임용우
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.21 no.1
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    • pp.39-44
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    • 2001
  • Growth characters and productivities of corn varieties were compared to provide the basic data to the famers that in order to select the good corn varieties at Deakwanryoung district of Korea. Currently cultivated 8 recommended corn varieties and Suwon 19ho as check variety were used in this experiment and experimental design was randomized complete block with three replications. Resarch results obtained at Deakwanryoung branch (sea love 800m) of National Livestock Research Institute in 1999 are summarized as follows. Culm lengths of DK501 and GL4899 among corn varieties were shorter as 180cm and 198cm, respectively and rest of varieties were more or less 230cm and no difference among varieties. Inflorescence height was lower in varieties showing low culm length, and inflorescence heights of PK3525 and DK729 were higher about 10~15cm than those of other varieties. Stem diameters of all varieties except DK501 were thicker compared to check variety, Suwon 19ho and P3144W was thickest. Leaf numbers of late maturing DK729 and G4743 were higher as average 12 and 12.3 per plant, respectively. Silking dates among varieties were ranged from August 10 to 20 showing 10 day difference and DK501 was earliest as August 10 and G4743 was latest as August 20. Ear rates of early silking varieties were more than 46% and late silking varieties were less than 40%(P>0.1). Dry matter and TDN yield of P3351 was highest as 24,710 and 17,470kg/ha respectively, and DK729 and G4743 were second and third in order. In conclusion. early and medium maturing variety. P3352 with high yield and good growth characters was desirable at Deakwanryoung district of Korea.

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Preliminary Studies on Production of Porcine Leucocyte Interferon (돼지 Leucocyte Interferon 생산(生産)에 대한 기초연구(基礎硏究))

  • Jun, Moo Hyung;Chung, Un Ik;Park, Bong Kyun;Kim, Kyo Joon;Lee, Hun Jun
    • Korean Journal of Agricultural Science
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    • v.12 no.1
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    • pp.153-161
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    • 1985
  • As a preliminary step for production of large quantities of porcine leucocyte interferon(Por IFN-${\alha}$), various factors such as the sources of leucocytes, inducers and culture conditions were investigated. In addition, an assay system for the potency of porcine interferon was developed in a micro-system by using porcine kidney cell line, PK-15, and vesicular stomatitis virus. By the experiments, it was found that the porcine leucocyte interferon was synthesized more effectively in the leucocyte suspension with the higher viability and the lesser erythrocyte contamination. Regarding cell sources, the peripheral leucocytes produced a consistently higher unit of interferon than the spleen cells. When the efficiency of inducers was compared, the Sendai virus at the concentration of 125 HA unit/ml was found to be more effective for porcine leucocyte interferon production than the Newcastle disease virus. Average potency of 9 batches of the crude interferon measured by the established assay system was 1,200 unit/ml.

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Preparation and Properties of Sulfonated Poly(ether ether ketone) (SPEEK) Electrospun Nanofibrous Ion-exchange Membrane for PEMFC (PEMFC용 설폰화 Poly(ether ether ketone) (SPEEK) 전기방사 나노섬유 이온교환막의 제조 및 특성)

  • Kwak, Noh-Seok;Choi, Eun-Jung;Hwang, Taek-Sung
    • Polymer(Korea)
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    • v.36 no.2
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    • pp.155-162
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    • 2012
  • Sulfonated poly(ether ether ketone) (SPEEK) nanofibers were prepared by electrospinning. The nanofibrous membrane for polymer electrolyte membrane fuel cell (PEMFC) was fabricated by compression molding. The maximum degree of sulfonation was 95% and the initial thermal degradation temperature was $280^{\circ}C$ and it's value was lower than that of PEEK. The contact angle of SPEEK increased with decreasing the degree of sulfonation. The optimum voltage, flow rate, tip to collector distance (TCD) and concentration of electrospinning conditions were 22 kV, 0.3 mL/hr, 15 cm, and 23 wt%, respectively. The average nanofibrous diameter was 47.6 nm. The water uptake and ion exchange capacity of SPEEK nanofibrous membrane increased with increasing the sulfonation time and the amount of sulfonating agent. The electrical resistance and proton ionic conductivity of SPEEK membrane increased with decreasing and increasing the sulfonation time, respectively. Their values were 0.58~0.06 ${\Omega}{\cdot}cm^2$and 0.099 S/cm.

A Study on the Effects of N , $P_2O_5$ and $K_2O$ Application on the Vegetation and Dry Matter Yield in the Mixed Sward of Oechardrass and Crabgrass ( Digitaria sanguinalis [L.] Scop.) (Orchargrass와 바랭이 ( Digitaria sanguinalis [ L. ] Scop ) 혼생초지에 있어서 질소 , 인산 및 가리시용이 식생 및 건물수량에 미치는 영향)

  • 김창주
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.15 no.2
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    • pp.93-100
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    • 1995
  • This experiment was carried out to investigate how to effect the application of nitrogen, phosphate and potassium on the vegetation and dry matter yield in the mixed sward of orchardgrass and crabgrass (Digitaria sanguinalis [L.] Scop.). The experimental sward wa5 consisted of eight plots of non-application, P, K, PK, N. NK, NP and NPK. These plots were sown with seeds mixture of orchardgrass 17.5 kglha and crabgrass 17.5 kg/ ha on 22 April 1975 in the forage experimental field of Kangwon National University, Chunchon, Korea, and were cut four times from 6 July to 18 November 1975. The results obtained were summarized as follows: 1. In orchardgrass density measured after summering, the plot of nitrogen applied without phosphate marked only 3 1.0% of the plot of nitrogen applied with phosphate while in crabgrass density measured at that time the plot of nitrogen applied without phosphate marked as high as 112.5% of the plot of nitrogen applied with phosphate. 2. Application of phosphate and potassium improved wintering record of orchardgrass. In orchardgrass density measured after wintering, the plot of nitrogen applied without phosphate marked only 9.7% of the plot of nitrogen applied with phosphate, and the plot of NP applied without K marked 63.6% of the plot of NP applied with K. 3. In surface coverage of orchardgrass, in midsummer any plot applied with nitrogen regardless of accompanying with or not with phosphate wa5 0%, but in autumn the plot of nitrogen applied with phosphate was 56-58% and the plot of nitrogen applied without phosphate was 5%. While after wintering the plot of nitrogen applied with phosphate was 28-37% though the plot of nitrogen applied without phosphate was only 0.3-0.6%; the application of phosphate improved summering and wintering record of orchardgrass. 4. Application of phosphate demonstrated remarkable effect on increasing dry matter yield in orchardgrass. In year total yield of orchardgrass, the plot of nitrogen applied without phosphate marked as low as 11.3% of the plot of nitrogen applied with phosphate, but in crabgrass the plot of nitrogen applied without phosphate was almost equal to the plot of nitrogen applied with phosphate showing 97.5% of this plot. 5. Application of potassium was not effective on increasing dry matter yield of orchardgrass but its application was effective on increasing dry matter yield of crabgrass. In dry matter yield of orchardgrass significant difference was not found between NP plot and NPK plot, but in dry matter yield of crabgrass NPK plot recorded significantly higher yield compared with NP plot (p<0.05). 6. In botanical composition of the plot of nitrogen applied with phosphate, crabgrass recorded 46.5-50.5% in early summer (July) but in midsummer (August) it dominated the plot recording 85.6-91.2%. 7. In botanical composition based on the year total yield, in the plot of nitrogen applied with phosphate orchardgrass marked 21.7-26.2% and crabgrass recorded 73.8-78.370, but in the plot of nitrogen applied without phosphate orchardgrass marked only 3.5% being overwhelmingly dominated by crabgrass which recorded 96.5%. 8. Application of nitrogen not accompanied with phosphate strengthened competitive power of the crabgrass unilaterally, making orchardgrass be oppressed fatally by the crabgrass.

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Preparation and Characterization of Electrospun PAN/TiO2 Fiber Mat by Electron Beam Irradiation (전자선 조사에 의한 PAN/TiO2 전기방사 나노섬유 제조 및 특성분석)

  • Kang, Phil-Hyun;Jeun, Joon-Pyo;Seo, Dong-Kwon;Kim, Hyun-Bin;Nho, Young-Chang
    • Polymer(Korea)
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    • v.36 no.1
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    • pp.47-52
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    • 2012
  • Abstract: In this study, PAN/$TiO_2$ fiber mats were fabricated from polyacrylonitrile (PAN) and titanium(IV) butoxide ($Ti(OBu)_4$) by an electrospinning method with various solution concentrations, applied voltages and solution flow rates. The fiber mats were irradiated with an electron beam to induce structural crosslinking and enhance photocatalytic activity. As a result, uniform and bead-free fibers without pits or cracks on surface were obtained at 5 wt% of $Ti(OBu)_4$ solution with 15 kV and 0.02 mL/min flow rate. The PAN/$TiO_2$ fiber mats were irradiated with an electron beam of 1.14 MeV acceleration voltage, 4 mA of current and $1{\times}10^4kGy$. Electron beam irradiation was enhanced the photocatalytic activity of PAN/$TiO_2$ nano fiber mat. The photocatalytic activity of the PAN/$TiO_2$ fiber mat was analyzed by degradation of methylene blue and volatile organic compounds.

Prediction of Internal Quality for Cherry Tomato using Hyperspectral Reflectance Imagery (초분광 반사광 영상을 이용한 방울토마토 내부품질 인자 예측)

  • Kim, Dae-Yong;Cho, Byoung-Kwan;Kim, Young-Sik
    • Food Engineering Progress
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    • v.15 no.4
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    • pp.324-331
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    • 2011
  • Hyperspectral reflectance imaging technology was used to predict internal quality of cherry tomatoes with the spectral range of 400-1000 nm. Partial least square (PLS) regression method was used to predict firmness, sugar content, and acid content. The PLS models were developed with several preprocessing methods, such as normalization, standard normal variate (SNV), multiplicative scatter correction (MSC), and derivative of Savitzky Golay. The performance of the prediction models were investigated to find the best combination of the preprocessing and PLS models. The coefficients of determination ($R^{2}_{p}$) and standard errors of prediction (SEP) for the prediction of firmness, sugar content, and acid content of cherry tomatoes from green to red ripening stages were 0.876 and 1.875kgf with mean of normalization, 0.823 and $0.388^{\circ}Bx$ with maximum of normalization, and 0.620 and 0.208% with maximum of normalization, respectively.