• Molecules and Cells
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• 제37권10호
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• pp.734-741
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• 2014

Histone modifications on major transcription factor target genes are one of the major regulatory mechanisms controlling adipogenesis. Plant homeodomain finger 2 (PHF2) is a Jumonji domain-containing protein and is known to demethylate the histone H3K9, a repressive gene marker. To better understand the function of PHF2 in adipocyte differentiation, we constructed stable PHF2 knock-down cells by using the mouse pre-adipocyte cell line 3T3-L1. When induced with adipogenic media, PHF2 knock-down cells showed reduced lipid accumulation compared to control cells. Differential expression using a cDNA microarray revealed significant reduction of metabolic pathway genes in the PHF2 knock-down cell line after differentiation. The reduced expression of major transcription factors and adipokines was confirmed with reverse transcription- quantitative polymerase chain reaction and Western blotting. We further performed co-immunoprecipitation analysis of PHF2 with four major adipogenic transcription factors, and we found that CCATT/enhancer binding protein (C/EBP)${\alpha}$ and C/EBP${\delta}$ physically interact with PHF2. In addition, PHF2 binding to target gene promoters was confirmed with a chromatin immunoprecipitation experiment. Finally, histone H3K9 methylation markers on the PHF2-binding sequences were increased in PHF2 knock-down cells after differentiation. Together, these results demonstrate that PHF2 histone demethylase controls adipogenic gene expression during differentiation.

• Nutrition Research and Practice
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• 제16권3호
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• pp.344-353
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• 2022

BACKGROUND/OBJECTIVES: Hydrolyzed formula is often fed to infants with gastrointestinal or immune issues, such as malabsorption or cow's milk allergy, because enzymatic treatment has rendered it more digestible and less allergenic than standard cow's milk formula (SF). Partially hydrolyzed formula (PHF) should be considered for those infants who are intolerant to extensively hydrolyzed formula. However, there are concerns about the nutritional insufficiencies of PHF. We aimed to evaluate the effects of PHF on the growth and health indicators in infants who were at high-risk of allergic disease and potential candidates for consuming PHF. SUBJECTS/METHODS: A total of 83 infants aged 0-2 mon with a family history of allergies were assigned to consume either PHF or SF until 24 weeks of age. Anthropometric measures were obtained at baseline, 12 weeks, and 24 weeks; blood samples were drawn and evaluated at the end of the study. RESULTS: No significant differences were observed in weight, height, and weight-for-height at any time point in each sex between the PHF and SF groups. At 24 weeks of age, the weight-for-age and height-for-age z-scores of the SF group were higher than those of the PHF group, but there was no significant difference in the weight-for-height z-score. There were no significant differences in levels of white blood cells, hemoglobin, ferritin, protein, albumin, aspartate aminotransferase, alanine aminotransferase, eosinophil cationic protein, and immunoglobulin E. CONCLUSIONS: In this study, there were no differences in growth and blood panels between the infants consuming PHF or SF. Therefore, infants who are unable to tolerate SF can be fed PHF without nutritional concerns about growth.

• Asian-Australasian Journal of Animal Sciences
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• 제31권11호
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• pp.1795-1806
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• 2018

Objective: The study was designed to establish choline deficiency model (CDM) in broilers for evaluating efficacy of polyherbal formulation (PHF) in comparison with synthetic choline chloride (SCC). Methods: A total of 2,550 one-day-old Cobb 430 broiler chicks were randomly assigned to different groups in three experiments. In experiment 1, G1 and G2 served as normal controls and were fed a basal diet with 100% soybean meal (SBM) as a major protein source supplemented with and without SCC, respectively. In G3, G4, G5, and G6 groups, SBM was replaced at 25%, 50%, 75%, and 100% by soy protein isolate (SPI) to induce a graded level of choline deficiency. In experiment 2, PHF (500 and 1,000 g/ton) in comparison with SCC (1,000 g/ton) were evaluated. In experiment 3, dose-response of PHF (200, 400, and 500 g/ton) with SCC (400 g/ton) was determined. Results: Replacement of SBM by SPI produced a linear decrease in body weight gain (BWG) with a poor feed conversion ratio (FCR). 25% SBM replacement by SPI yielded an optimum negative impact on BWG and FCR; hence, it is considered for further studies. In experiment 2, PHF (500 and 1,000 g/ton) and SCC (1,000 g/ton) showed a similar performance in BWG, FCR and relative liver weight. In experiment 3, PHF produced an optimum efficacy at 400 g/ton and was comparable to SCC in the restoration of serum aspartate aminotransferase activity, abdominal fat, breast muscle lipid content and liver histopathological abnormalities. Conclusion: Replacement of SBM by SPI caused choline deficiency characterised by worsening of BWG, FCR, elevation in liver enzymes and histopathological changes indicating fatty liver. CDM was found valid for evaluating SCC and PHF. It is concluded that PHF has the potential to mimic biological activities of SCC through the restoration of negative effects caused by CDM.

• 동아시아식생활학회지
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• 제26권6호
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• pp.517-530
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• 2016

This research was conducted on Seoul region elementary, middle, and high school nutritionists to analyze execution conditions of HACCP control standards, focusing on PHF and CCP 3~CCP 7, in order to determine more efficient methods for school meals' sanitation system settlement. All surveys were distributed and collected via email. A total of 305 survey papers were collected, and 300 school results were analyzed. The following percentage of nutritionists perceived the following foods as potentially hazardous foods(PHF): raw or cooked animal foods (94.7%), blossomed seed products (93.7%), packed pickled radish (36.7%), unopened mayonnaise (30.2%), and unopened snails & corn cans 54.8%. Exactly 51.2% of nutritionists believed that foods were properly supplied via CCP 3's food quality standard. Exactly 33.9% of nutritionists answered that they had never corrected the digital thermometer since there were no issues with the CCP 4's digital thermometer correction frequency level. As for CCP 5's chroline disinfection of green vegetables, 57.1% of nutritionists answered that vegetables were slightly softened while 36.2% said there were no changes at all. According to the nutritionists, the most difficult execution level of CCP levels (excluding CCP 3, CCP 4) was CCP 7 (37.1%), CCP 6 (16.4%), and CCP 2 (16.4%). For the above results, proper training/education must be enforced so that nutritionists can have a clear notion of the PHF. A solution must be developed enhancing the execution of CCP 4's digital thermometer correction frequency level. For CP 5's chroline disinfection of green vegetables, food characteristics must be considered to suggest an appropriate number and method of cleansing. Furthermore, cooking employees are needed that properly use and manage cooling & heating equipments to maintain heated foods above $57^{\circ}C$ under the CCP 7 standard.

• 한국식품위생안전성학회지
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• 제21권3호
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• pp.136-144
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• 2006

본 연구에서는 초등학교 급식에서 잠재적 위해식품을 포함한 급식메뉴의 기호도와 섭취현황을 분석하였다. 3주 동안의 메뉴에서 고단백음식과 생야채 또는 조리된 야채 메뉴, 과일 등을 잠재적 위해식품 1과 2로 각각 명시하였다. 세 곳 초등학교에서 10세에서 12세 학생들을 대상으로 학생들에게 그날의 급식을 얼마나 먹었는지, 어떤 음식을 가장 선호하는지, 좋아하지 않는 음식은 무엇이며, 왜 그 음식을 좋아하지 않는지 등을 설문 조사하였다. 학교 간, 학년 간, 성별간의 음식섭취량에 유의적 차이가 있는지를 SPSS을 사용하여 카이제곱 검정을 하였다. 결과에 따르면, 학생들은 적어도 급식을 통해 하루에 두 종류의 잠재적 위해식품군에 노출되는 것으로 나타났다. 조사대상자의 89%와 82%의 학생들이 잠재적 위해식품 1과 2를 각각 100% 다 섭취하는 것으로 나타났다. 또한 학교와 요일에 따라 학생들의 음식섭취량에는 유의적 차이를 나타내었다. 학생들은 고단백식이, 특히 미트볼조림, 돼지고기, 닭고기 바베큐를 선호하고 섭취 또한 많이 하는 것으로 나타났다 이번 연구 결과는 학교급식에서의 식중독 위험성을 감소하기 위해서는 급식 메뉴에서 잠재적 위해식품과 관련된 위해요소를 관리할 수 있는 방법 또는 공정의 필요성을 시사한다.

• 대한영양사협회학술지
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• 제14권2호
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• pp.176-185
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• 2008

The purpose of this study was to identify school dieticians' perceptions on the Potentially Hazardous Food (PHF) and the inspection of food safety and sanitation conducted by Provincial Office of Education. Questionnaires were distributed to 400 school dieticians in elementary schools in Seoul and Gyeonggi Province, and 217 responses were collected. The majority of respondents (64%) defined PHF as food that easily decays at room temperature and over half of the respondents considered Korean cooked vegetables as a PHF. In addition, 4% of respondents completely excluded PHFs from their menus. Forty five percent of the respondents selected CCP rule 7 (delivery and distribution process) and 34% selected CCP rule 6 as the most difficult of the CCP rules to follow. Also, perceptions concerning food safety inspection and sanitation were not high in terms of validity, objectivity, and reliability among the evaluation criteria. The study results suggest that PHF as well as Potentially Hazardous Menu (PHM) definitions and guidelines should be re-established in consideration of traditional Korean food culture and the preparation of Korean foods.

• 한국버섯학회지
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• 제4권2호
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• pp.48-52
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• 2006

느타리는 4극성 자웅이주성으로 단포자분리주는 단핵으로 자실체를 형성하지 못하는 것으로 알려져 있다. 그러나 단포자분리주는 2종류로 구분되었는데 하나는 자실체를 완전히 형성하지 못하는 AHF형 (abortive homokaryotic fruiting, AHF) 이며, 나머지 하나는 원기형성 후 완전한 자실체로 발달하는 PHF형 (Pseudo- homokaryotic fruiting, PHF)이었다. 느타리 8개 계통으로부터 단포자분리주 155개를 얻어 단핵체 자실체를 유도하여 이중에서 102개임성체를 얻었으며, 이는 65.8%에 해당되었다. 단핵 자실체는 이핵체 모균주에 비해 대부분 자실체가 빈약하였으나 일부 모균주와 유사한 크기의 자실체도 발생하였다. 이들 단핵 임성균주를 이용하여 계통간 교잡조합 11개에서 535 교잡체와 비임성균주간 교잡체 79개를 얻어 톱밥병재배를 하여 자실체 생산력을 모균주와 조사 비교하였다. 자실체 생육일수는 임성주가 비임성주간 교잡 집단에 비해 빨랐다. 병당 유효경수는 임성체간 교잡주가 모균주나 비임성교잡체보다 많았으며, 대길이도 두 집단에 비해 길었다. 그러나 대굵기와 자실체 개체중은 3개 집단간 거의 차이가 없었다. 단핵 임성체간 교잡주 집단의 병당 자실체 수량이 비임성간과 모균주 집단보다 높은 경향이었다. 모균주 집단의 자실체 수량지수를 100으로 하였을 때 임성체간 교잡체 집단은 105, 비임성간 교잡체 집단은 86으로 나타났다. 특히 $2194{\times}2344$ 조합은 59.95%, $2194{\times}2730$ 조합은 27.39%, $1982{\times}2504$ 조합은 22.43% 양친균주보다 자실체 수량이 증수되는 것으로 나타났다. 따라서 단핵 임성체를 이용한 우수 균주개발 가능성은 높다고 할 것이다.

• Animal cells and systems
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• 제1권2호
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• pp.317-321
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• 1997

Tau protein is one of the microtubule-associated proteins in the mammalian brain. In Alzheimer's disease, tau protein is immobilized in the somatodendritic compartment of certain nerve cells, where it forms a part of the paired helical filament (PHF). To understand the role of tau protein in the formation of PHF, a recombinant human tau protein expressed in Escherichia coli and five synthetic peptide fragments (peptide 1 to peptide 5), corresponding to the C-terminal region of tau protein, were prepared and their ability in self-assembly to form filamentous structures was examined. The recombinant human tau protein formed short rod-like structures in 0.1M MES buffer containing 1 mM $MgCI_2$, while a synthetic peptide fragment 1 containing 55 amino acid residues could assemble into a lot of long filamentous structures in water and particularly twisted helical structures in 0.1M MES buffer containing 1 mM $MgCI_2$. This suggests that the C-terminal region possesses a filament-forming ability and may be related to the formation of the helical structure by providing a powerful filament-forming driving force.

• 한국식품과학회지
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• 제45권1호
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• pp.59-69
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• 2013

식중독의 원인이 될 수 있는 가능성이 있는 식품을 잠재적 위해식품이라 하며 이 식품들의 섭취행태가 정확이 파악되어야 정확한 미생물 위해평가를 할 수 있다. 본 연구는 50가지 잠재적 위해식품을 선정하고 대한민국의 성인을 대상으로 잠재적 위해식품의 섭취 실태를 조사하기 위해 전국의 성인 남녀 1,000명을 5월과 8월에 나누어 설문조사를 하였다. 월 1회 이상 섭취하는 빈도를 보았을 때 쌈야채는 소비자의 91.3%가 월 1회 이상 자주 섭취하는 것으로 가장 높게 나타났으며 데친 나물류는 72.3%, 닭튀김은 68.1%였으며 그 뒤로 파채(67.9%), 맛살/어묵(67.3%), 부추(65.3%), 삼각김밥(62.5%), 계란지단/찜(62.4%), 샐러드(59.8%), 오징어/문어(58.7%) 순으로 나타났다. 소비자의 위험인식도가 가장 높은 식품은 조개로 4.36점으로 조사되었으며 홍합(4.35점), 생굴(4.30점), 육회(4.29점), 생선회(4.27점), 해삼/멍게(4.19점), 꼬막(4.16점), 참치회(4.10점), 초밥(4.08점), 날치알(3.95점)의 순서로 나타나 수산물 및 수산가공품의 비율이 높게 나타났으며 이들의 위험인식도는 5월보다 8월이 더 높게 나타나 기온이 올라감에 따라 소비자들의 위험 인식도가 증가함을 볼 수 있다. 반면에 소비자는 위험하다고 생각하지 않는 식품으로는 부추가 1.93점으로 가장 낮게 나타났으며 쌈야채(2.00점), 파채(2.02점), 새싹채소/무순(2.12점), 생식/선식(2.40점), 샐러드(2.40점), 데친나물류(2.47점), 오징어채/포류(2.54점), 조미쥐포류(2.59점), 육포(2.63점) 순으로 나타났으며 미생물의 오염도가 높음에도 불구하고 위험 인식도가 낮아 이들 식품의 위험성에 대한 교육이 필요한 것으로 나타났다. 잠재적 위해식품의 섭취실태조사는 식중독의 원인이 될 수 있는 식품의 위해도 분석 시 기초자료가 될 수 있으며 소비자들의 위험 인식 수준에 대해 지각하고 식중독 예방교육의 방향을 제시해 줄 수 있다.

• 한국식품영양학회지
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• 제27권3호
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• pp.447-457
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• 2014

A research was conducted on Seoul regional elementary, middle, and high school nutritionists to study about their perception of HACCP control standards & performance conditions and sanitation & safety inspection, to seek for more efficient methods of school meals' sanitation system settlement. All surveys were distributed and collected via email. A total of 305 survey papers were collected, and out of these, 300 school results were analyzed. As for CCP 1 performance conditions, 43.3% of the nutritionist put emphasis on temperature control for cooking duration and 71.0% said that they manage both temperature and PHF food control. In CCP 2 stage, 65.8% of the nutritionists maintained the food's temperature, and 56.7% documented the recordings after cooking. A total of 79.3% of the schools scored above 90 points on school meal sanitation & safety inspection, 3.72 points on necessity for revisions, 3.38 points on objectivity, and 3.34 points on reliability. As for these results, a clear CCP control criteria as well as training must be set. Also, because the necessity of revision for sanitation & safety inspection is higher than reliability and objectivity, appropriate complementary measures must be taken.