• 생명과학회지
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• 제16권4호
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• pp.605-611
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• 2006

양식 산업의 문제점인 어병균을 방제하기 위한 생물 전구체의 개발을 목적으로 해산어의 내장 추출물에서 어병균인 Vibrio anguillarum의 생육을 저해하는 새로운 균주를 분리, 동정하여 Bacillus amyloliquefaciens H41으로 명명하고 생육특성 및 생육저해인자 생성 조건을 검토하였다. 분리 균의 생육 최적 조건은 기본배지로 1% peptone, 질소원으로 1.5% yeast extract, 탄소원으로 1% sucrose, 0.5% NaCl, 금속이온으로는 0.05% $MgSO_4{\cdot}7H_20$이었으며 pH는 7.0-8.0, 온도는 $28-35^{\circ}C$에서 20시간의 진탕배양이었다. V. anguillarum 생육 저해물질은 B. amyloliquefaciens H41의 배양 상등 액에서 볼 수 있었는데 paper-disk 법으로 투명환의 생성여부로 확인하였다. 분리 균에 의한 생육저해 물질 생산 최적 조건은 1% peptone, 1.5% yeast extract, 1% NaCl, 0.05% $MgSO_4{\cdot}7H_20$, 1%의 sucrose이었으며 최적 pH는 7.5, 최적온도는 $35^{\circ}C$이었다. 분리 균은 최적 배양조건하에서 저해물질을 배양 후 16시간부터 24시간대에 가장 높은 활성을 나타내었다. 저해 활성반응은 분리 균에서는 저해 활성을 나타내었으나 B. amyloliquefaciens KCTC1724 표준균주에는 저해활성을 나타나지 않는 대조를 보였다. 또한 분리 균에 의해 생산된 저해물질은 V. anguillarum 에는 생육 억제 효과를 나타내었으나 다른 인체 병원성 Vibrio에는 전혀 영향을 미치지 않는 것으로 나타나 균주 선택성이 특이적이었다.

• Journal of Microbiology and Biotechnology
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• 제32권5호
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• pp.630-637
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• 2022

The objective of this study was to optimize industrial-grade media for improving the biomass production of Weissella cibaria JW15 (JW15) using a statistical approach. Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K₂HPO₄, potassium citrate, ⳑ-cysteine phosphate, MgSO₄, and MnSO₄) were screened by using the Plackett-Burman design. Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM). The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K₂HPO₄, 5.0 g/l sodium acetate, 0.1 g/l MgSO₄·7H₂O, 0.05 g/l MnSO₄·H₂O, and 1.0 g/l Tween 80. The OM significantly improved the biomass production of JW15 over an established commercial medium (MRS). After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l). Correspondingly, JW15 showed a rapid and increased production of lactic and acetic acid in the OM. To perform a scale-up validation, batch fermentation was executed in a 5-l bioreactor at 37℃ with or without a pH control at 6.0 ± 0.1. The biomass production of JW15 significantly improved (1.98 times higher) under the pH control, and the cost of OM was reduced by two-thirds compared to that in the MRS medium. In conclusion, OM may be utilized for mass producing JW15 for industrial use.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 1975년도 추계학술발표회
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• pp.182.3-183
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• 1975

본품에 의한 단백질 부해효소의 생산조건, 정제 및 특성을 조사해본 결과는 다음과 같다. 1) 0.5％ nylon oligomer, 0.3％ peptone, 0.1％ yeast extract 및 salt mixture를 함유한 배지에서 24시간 진탕배양 했을 때 가장 높은 생산율을 보여주었다.(중략)

• Journal of Microbiology and Biotechnology
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• 제19권4호
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• pp.378-386
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• 2009

A sequential optimization strategy, based on statistical experimental designs, is employed to enhance the production of alkaline protease by a Bacillus pseudofirmus local isolate. To screen the bioprocess parameters significantly influencing the alkaline protease activity, a 2-level Plackett-Burman design was applied. Among 15 variables tested, the pH, peptone, and incubation time were selected based on their high positive significant effect on the protease activity. A near-optimum medium formulation was then obtained that increased the protease yield by more than 5-fold. Thereafter, the response surface methodology(RSM) was adopted to acquire the best process conditions among the selected variables, where a 3-level Box-Behnken design was utilized to create a polynomial quadratic model correlating the relationship between the three variables and the protease activity. The optimal combination of the major medium constituents for alkaline protease production, evaluated using the nonlinear optimization algorithm of EXCEL-Solver, was as follows: pH of 9.5, 2% peptone, and incubation time of 60 h. The predicted optimum alkaline protease activity was 3,213 U/ml/min, which was 6.4 times the activity with the basal medium.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.129-132
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• 2000

유기산 생산을 위한 Propionibacterium acidipropionici의 발효 특성을 규명하기 위하여 ATCC 4965, 4875, 25562의 세 균주를 선별하였다. 세포 성장에 따른 발효 실험에서는 Peptone, Yeast extract, pH별로 실시되었으며, 각각 1.5 %, 0.75%, $5.5{\sim}7.5$에서 유기산 생산성이 최적으로 나타났다. 회분식 발효결과 세 균주 모두 pH 6.0에서 유기산 수율과 생산성이 pH 7.0에 비해 더 높았으며, 그 중에서도 P.acidipropionci ATCC4965균주가 유기산 생산성이 0.29g total acids/L/h로 가장 우수한 것으로 나타났다.

• KSBB Journal
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• 제7권2호
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• pp.112-117
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• 1992

Streptococcus zooepidemicus에 의한 hyaluronic acid 생성의 최적 배지 조성은 batch cultre 조건에서 0.1% 쇠고기 추출물, 0.1% 효모 추출물, 3.0% 포도당 2.0% peptone, 0.1% 식염 및 0.5% $CaCO_3$이였으며, 배지의 포기 pH는 7.5로서 $37^{\circ}C$에서 36시간 진탕배양하는 것이 양호했다. 특히 공시균의 생육에 수반되어 hyaluronic acid가 생성되므로 배지의 pH를 중화하기위해 $CaCO_3$의 첨가는 필수적이었다.

• 한국식물병리학회지
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• 제1권1호
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• pp.56-60
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• 1985

본 실험은 질소 및 탄소원이 Cercospora kikuchii 균사생장에 미치는 영향과 여러 배양조건에서 생장한 C. kikuchii가 세균생장을 저지하는 정도를 알아보았다. C. kikuchii 균사생장에 적합한 질소원은 yeastextract였고, 탄소원으로는 sucrose, glucose, maltose, fructose를 첨가하였을 때 균사생장이 좋았다. C. kikuchii의 세균생장저지 실험에서 wild type은 pH 조건은 5.0에서, 질소원으로는 yeast-extract 또는 peptone.을 첨가하였을 때, 탄소원으로는 sucrose, maltose, glucose, fructose를 첨가한 배지에서 생장하였을 때 Erwinia carotovora와 Pseudomonas solanacearum의 생장을 크게 저지시켰다. 그러나 albino 돌연변이균주는 실허된 세균의 생장을 거의 저지시키지 못하였다.

• Applied Biological Chemistry
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• 제47권1호
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• pp.22-26
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• 2004

리그닌 분해균인 느타리 버섯균(Pleurotus ostreatus) K-2946을 glucose-peptone-yeast(G-P-Y) 액체배지에서 배양 하여 망간퍼옥시데이즈를 생산하였다. 본 실험조건 하에서 리그닌 퍼옥시데이즈는 생산되지 않았다. 느타리버섯균 K-2946이 생산하는 망간 퍼옥시데이즈를 이온크로마토그래피, 겔크로마토그래피를 이용하여 정제하였다. 정제한 망간 퍼옥시데이즈의 분자량은 36400 Da이였고 pI는 3.95였다. 정제된 효소의 최적 pH는 5.0이었으며 최적온도는 $55^{\circ}C$이었다.

• Mycobiology
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• 제38권3호
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• pp.171-175
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• 2010

Metacordyceps yongmunensis is a newly reported species from Korea, which is very similar to Cordyceps species in morphological characters. It grows on large lepidopteran pupa, and numerous white stromata grow on a single host. Mycelial growth characteristics of M. yongmunensis isolates were studied in different media and at different temperatures. Also, different carbon sources, nitrogen sources, and mineral salts were tested for mycelial growth of M. yongmunensis. Schizophyllum (mushroom) genetics complete medium plus yeast extract, Schizophyllum (mushroom) genetics minimal medium, and Martin's peptone dextrose agar produced longer colony diameters and more compact mycelial density than other media. The optimum temperature for mycelial growth was $25^{\circ}C$. Carbon sources such as sucrose, soluble starch, dextrose, glucose, dextrin, maltose, and fructose showed better mycelial growth, whereas peptone, yeast extract and tryptone resulted in the best mycelial growth of all of the nitrogen sources tested. All of the mineral salts tested showed similar growth as the control, except $K_2HPO_4$ which showed longer colony diameter and more compact mycelial density. The compact colonies were white and cottony with a greenish margin. The results showed that M. yongmunensis is an easy fungus to growas it grew from 30 to more than 50 mm in 2 wk.

• Journal of Microbiology and Biotechnology
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• 제20권4호
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• pp.835-843
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• 2010

The objectives of this study were to investigate the effect of fermentation medium on the hydroxyl radical scavenging activity of exopolysaccharides from Inonotus obliquus by response surface methodology (RSM). A two-level fractional factorial design was used to evaluate the effect of different components of the medium. Corn flour, peptone, and $KH_2PO_4$ were important factors significantly affecting hydroxyl radical scavenging activity. These selected variables were subsequently optimized using path of steepest ascent (descent), a central composite design, and response surface analysis. The optimal medium composition was (% w/v): corn flour 5.30, peptone 0.32, $KH_2PO_4$ 0.26, $MgSO_4$ 0.02, and $CaCl_2$ 0.01. Under the optimal condition, the hydroxyl radical scavenging rate (49.4%) was much higher than that using either basal fermentation medium (10.2%) and single variable optimization of fermentation medium (35.5%). The main monosaccharides components of the RSM optimized polysaccharides are rhamnose, arabinose, xylose, mannose, glucose, and galactose with molar proportion at 1.45%, 3.63%, 2.17%, 15.94%, 50.00%, and 26.81%.