• Title/Summary/Keyword: PEL

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Effect of Carbon Sources and Culture Temperature on Pectate Lyase Production in Phytopathogenic Bacteria (탄소원과 배양온도가 식물 병원세균의 Pectate lyase 생산에 미치는 영향)

  • 한광섭;최재을
    • Korean Journal Plant Pathology
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    • v.14 no.2
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    • pp.125-129
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    • 1998
  • Phytopathogenic bacteria causing soft-rot many vegetables; extracellular enzymes produced by them, pectate lyase(Pel) is important pathogenicity facotrs which cause tissue maceration and cell death. Ten of seventeen plant pathogenic bacteria showed weak Pel activity, four of them showed low Pel activity and Erwinia acrotovora subsp. carotovora, E. chrysanthemi, Pseudomonas marginalis and Xanthomonas campestris pv. campestris showed high Pel activity in the polygalacturonate yeast extract agar (PAY) plate. High Pel activity of the four bacteria species produced the highest Pel activity when pectin or polygalacturonic acid (PGA) was added to minimal salts (MS) medium. Pel activity of the four bacterial species was the highest at 2$0^{\circ}C$ among different temperature conditions. The rate and amount of maceration of potato tuber tissue were highest at 2$0^{\circ}C$ in E. carotovora subsp. carotovora, E. chrysanthemi and P. marginalis, while those were the highest at $25^{\circ}C$ in X. campestris pv. campetris.

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Plant Cell Wall as an Inducer of Pectate Lyase of Erwinia rhapontice (Erwinia rhapontici의 Pectate Lyase를 유도하는 식물 세포벽)

  • 최재을;한광섭
    • Korean Journal Plant Pathology
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    • v.12 no.1
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    • pp.129-131
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    • 1996
  • Pecatate lyase(Pel)는 펙틴과 펙틴산을 분해하며, 기주식물의 감염에 관여한다. Erwinia rhapontici에 있어서 기주와 병원균이 병원성과의 상호관계를 구명하기 위하여 pectate lyase(Pel) 활성에 미치는 식물체 추출물과 세포벽의 효과를 검토하였다. 본 균은 glycerol이 포함된 minimal salts(MSG) 배지와 식물체 추출물이 첨가된 MSP 배지에서는 Pel 활성이 검출되지 않았다. 그러나 배추, 상추 잎, 감자 괴경, 셀러리 잎자루, 양파 인경, 당근 뿌리의 세포벽이 첨가된 MSP 배지에서는 Pel의 활성이 검출되었다. Pel을 유도하는 식물 인자는 불용성이고, 열처리에 불안전하였다.

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Variable Sub-pixel Motion Vector Resolution Based on Block Mode Motion Estimation for H.264/AVC

  • Tran, Trung-Kien;Kim, Dae-Yeon;Lee, Yung-Lyul
    • Proceedings of the IEEK Conference
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    • 2008.06a
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    • pp.807-808
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    • 2008
  • In H.264, sub-pel motion estimation (ME) has strong effect when coding video sequences. 1/4-pel performs better at low bitrate while 1/8-pel gives better results at high bitrate. In this paper, a variable sub-pixel motion vector resolution based on block mode motion estimation method is proposed. Experiment results show that the proposed method can take the advantage of 1/4-pel at low bitrate and 1/8-pel at high bitrate. In addition to that, time is reduced from 14% to 53% compared to KTA1.3 with 1/8-pel motion vector (MV) resolution.

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Analysis of cel and pel Genes from Pectobacterium chrysanthemi PY35 for Relatedness to Pathogenicity

  • Park, Sang-Ryeol;Lim, Woo-Jin;Kim, Min-Keun;Hong, Su-Young;Shin, Eun-Chule;Kim, Eun-Ju;Lee, Jong-Yeoul;Woo, Jong-Gyu;Kim, Hoon;Yun, Han-Dae
    • Journal of Microbiology and Biotechnology
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    • v.14 no.5
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    • pp.1047-1051
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    • 2004
  • The phytopathogenic bacterium Pectobacterium chrysanthemi secretes multiple isozymes of plant cell wall disrupting enzyme such as pectate lyase and cellulase. The cel gene, existing in tandem with the pel gene, was isolated previously [10]. The role of Cel5Z and PelL1 in P. chrysanthemi PY35 pathogenicity on potato tissues was assessed by mutagenizing cloned cel gene and pel gene in tandem and recombining them with the chromosomal alleles. Strains with the Km cassette interposon in pelL1 or a double mutant showed a delay in the appearance of symptoms, suggesting that P. chrysanthemi PY35 pectate lyase PelL1 may playa minor role in soft-rot pathogenesis.

Susceptibility of KSHV-Infected PEL Cell Lines to the Human Complement System

  • Yoo, Seung-Min;Jeon, Hyungtaek;Lee, Suhyuk;Lee, Myung-Shin
    • Journal of Microbiology and Biotechnology
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    • v.26 no.3
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    • pp.618-626
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    • 2016
  • Pleural effusion lymphoma (PEL) is a rare B-cell lymphoma that has a very poor prognosis with a median survival time of around 6 months. PEL is caused by Kaposi's sarcoma-associated herpesvirus, and is often co-infected with the Epstein Barr virus. The complement system is fundamental in the innate immune system against pathogen invasion and tumor development. In the present study, we investigated the activation of the complement system in PEL cells using human serum complements. Interestingly, two widely used PEL cell lines, BCP-1 and BCBL-1, showed different susceptibility to the complement system, which may be due to CD46 expression on their cell membranes. Complement activation did not induce apoptosis but supported cell survival considerably. Our results demonstrated the susceptibility of PEL to the complement system and its underlying mechanisms, which would provide insight into understanding the pathogenesis of PEL.

Marker-Exchange Mutagenesis of Pectate Lyase Gene in Rhizobium fredii (Rhizobium fredii Pectate Lyase 유전자의 Marker-Exchange 변이)

  • 정민화;박용우;윤한대
    • Microbiology and Biotechnology Letters
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    • v.19 no.3
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    • pp.222-227
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    • 1991
  • Rhizobium fredii USDA193 is one of the causal organism for root nodule formation in soybean (peking). Previously we cloned the pectate lyase gene (SY1) of R. fredii USDA193. The $pel^-$ mutants (SY1$\Omega$ and SY1$\Omega$1) of SY1 were obtained using the in vitro insertional omega mutagenesis of RpelB (of Rhizobium pel) and fill-in reaction of RpelE (of Rhizobium pel) gene respectively, and we constructed two mutants (R, fredii USDA193$\Omega$ and R. fredii USDA193$\Omega$1) in pectate lyase function by marker-exchange with pe1B::$\Omega$ and R. fredii USDA193 strain (rif). The pectate lyase activity of two pel- mutant of R. fredii USDA193 was determined by spectrophotometric method. However, all pectate lyase activity of these mutants was not lost upon the mutagenesis by marker-exchange. This suggests that other pectate lyase genes may be present on the plasmid or the chromosome of R. fredii. As yet we do not have evidence linking RpelB and RpelE genes of R. fredii directly to the early nodulation process.

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A New Proposal of Adaptive BTC for Image Data Compression (畵像壓縮을 위한 適應 BTC 方法의 提案)

  • Jang, Ki-Soong;Oh, Seong-Mock;Lee, Young-Choul
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.26 no.7
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    • pp.125-131
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    • 1989
  • This paper proposes a new ABTC (Adaptive Block Truncation Coding) algorithm which improves the BTC algorithm for image data compression. A new adaptive block truncation coding which adopts a selective coding scheme depending on the local characteristics of an image has been described. The characteristics of the ABTC algorithm can be summarized as high compression ratio and the algorithm simplicity. Using this algorithm, color images can be coded at a variable bit rate from 1.0 (bit/pel) to 2.56 (bit/pel) and high compression rate (1.3-105 bit/pel) can be achieved without conspicuous image degradation compared with original images.

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Half-pixel Accuracy Motion Estimation Using the Correlation of Motion Vectors (움직임벡터의 상관성을 이용한 반화소단위 움직임 추정 기법)

  • Lee, Bub-Ki;Lee, Kyeong-Hwan;Choi, Jung-Hyun;Kim, Duk-Gyoo
    • Journal of the Korean Institute of Telematics and Electronics S
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    • v.35S no.6
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    • pp.119-126
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    • 1998
  • In this paper, we proposed two new methods of half-pel accuracy motion estimation using spatial correlation of half-pel accuracy motion vectors and stochastic characteristics between pixel accuracy motion vectors and half-pel accuracy motion vectors. We confirmed two facts : One is that the probability of having same half-pel accuracy motion vectors with neighboring blocks is high when having same pixel accuracy motion vectors. And the other is that there is high correlation between neighboring half-pel positions. These new half-pel motion estimation technique are shown to decrease the bit rates for vector coding and computational complexity with similar PSNR.

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Fast Block Matching Algorithm With Half-pel Accuracy for Video Compression (동영상 압축을 위한 고속 반화소 단위 블록 정합 알고리듬)

  • 이법기;정원식;김덕규
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.24 no.9B
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    • pp.1697-1703
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    • 1999
  • In this paper, we propose the fast block matching algorithm with half pel accuracy using the lower bound of mean absolute difference (MAD) at search point of half pel accuracy motion estimation. The proposed method uses the lower bound of MAD at search point of half pel accuracy which calculated from MAD's at search points of integer pel accuracy. We can reduce the computational complexity by executing the block matching operation only at the necessary search point. The points are selected when the lower bound of MAD at that point is smaller than reference MAD of integer pel motion estimation. Experimental results show that the proposed method can reduce the computational complexity considerably and keeping the same performance with conventional method.

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Efficient Integer pel and Fractional pel Motion Estimation on H.264/AVC (H.264/AVC에서 효율적인 정화소.부화소 움직임 추정)

  • Yoon, Hyo-Sun;Kim, Hye-Suk;Jung, Mi-Gyoung;Kim, Mi-Young;Cho, Young-Joo;Kim, Gi-Hong;Lee, Guee-Sang
    • The KIPS Transactions:PartB
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    • v.16B no.2
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    • pp.123-130
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    • 2009
  • Motion estimation (ME) plays an important role in digital video compression. But it limits the performance of image quality and encoding speed and is computational demanding part of the encoder. To reduce computational time and maintain the image quality, integer pel and fractional pel ME methods are proposed in this paper. The proposed method for integer pel ME uses a hierarchical search strategy. This strategy method consists of symmetrical cross-X pattern, multi square grid pattern, diamond patterns. These search patterns places search points symmetrically and evenly that can cover the overall search area not to fall into the local minimum and to reduce the computational time. The proposed method for fractional pel uses full search pattern, center biased fractional pel search pattern and the proposed search pattern. According to block sizes, the proposed method for fractional pel decides the search pattern adaptively. Experiment results show that the speedup improvement of the proposed method over Unsymmetrical cross Multi Hexagon grid Search (UMHexagonS) and Full Search (FS) can be up to around $1.2{\sim}5.2$ times faster. Compared to image quality of FS, the proposed method shows an average PSNR drop of 0.01 dB while showing an average PSNR gain of 0.02 dB in comparison to that of UMHexagonS.