Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Analysis of cel and pel Genes from Pectobacterium chrysanthemi PY35 for Relatedness to Pathogenicity

  • Park, Sang-Ryeol (National Institute of Agricultural Biotechnology) ;
  • Lim, Woo-Jin (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Min-Keun (Division of Applied Life Science, Gyeongsang National University) ;
  • Hong, Su-Young (Division of Applied Life Science, Gyeongsang National University) ;
  • Shin, Eun-Chule (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Eun-Ju (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Jong-Yeoul (National Institute of Agricultural Biotechnology) ;
  • Woo, Jong-Gyu (National Horticultural Research Institute, RDA) ;
  • Kim, Hoon (Department of Agricultural Chemistry, Sunchon National University) ;
  • Yun, Han-Dae (Division of Applied Life Science, Gyeongsang National University, Institute of Agriculture & Life Scineces, Gyeongsang National University)
  • Published : 2004.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The phytopathogenic bacterium Pectobacterium chrysanthemi secretes multiple isozymes of plant cell wall disrupting enzyme such as pectate lyase and cellulase. The cel gene, existing in tandem with the pel gene, was isolated previously [10]. The role of Cel5Z and PelL1 in P. chrysanthemi PY35 pathogenicity on potato tissues was assessed by mutagenizing cloned cel gene and pel gene in tandem and recombining them with the chromosomal alleles. Strains with the Km cassette interposon in pelL1 or a double mutant showed a delay in the appearance of symptoms, suggesting that P. chrysanthemi PY35 pectate lyase PelL1 may playa minor role in soft-rot pathogenesis.

Keywords

References

  1. Beaulieu,C., M. Boccara, and F. V. Gijsegem. 1993.Pathogenic behaviour of pectinase-defective Erwinia chrysanthemi mutants on different plants: Possible involvement of pectic enzymes in host specificity. Mal. Plant-Microbe Interact. 6: 197- 202.
  2. Beguin, P. 1990. Molecular biology of cellulose degradation. Annu. Rev. Microbial. 44: 219- 248.
  3. Hinton, J. C. D., J. M. Sidebotham, D. R. Gill, and G. P. R. Salmond. 1989. Isolation and characterization of transposon-induced mutants of Erwinia carotovora subsp. atroseptica exhibiting reduced virulence. Mal. Microbial. 3: 1785- 1795.
  4. Hong, I. P., H. K. Jang, S. Y. Lee, and S. G. Choi. 2003. Cloning and characterization of a bifunctional cellulasechitosanase gene from Bacillus lichenifarmis NBL420. J. Microbiol. Biotechnol. 13: 35- 42.
  5. Johnvesly, B., S. Virupakshi, G. N. Patil, A. Ramalingam, and G. R. Naik. 2002. Cellulase-free thermostable alkaline xylanase from thermophilic and alkalophilic Bacillus sp. JB-99. J. Micr.obiol. Biotechnol. 12: 153- 156.
  6. Kim, M. K., S. R. Park, S. J. Cho, W. J. Lim, S. K. Ryu, C. R. An, S. Y. Hong, Y. W. Park, G. G. Kahng, J. H. Kim, H. Kim, and H. D. Yun. 2002. The effect of the disrupted yhjQ on cellular morphology and cell growth in Escherichia coli. Appl. Microbial. Biotechnol. 60: 134- 138.
  7. Koutonjansky, A., A. Diolez, M. Boccara, Y. Bertheau, T. Andro, and A. Coleno. 1985. Molecular cloning of Erwinia chrysanthemi pectinase and cellulase structural genes. EMBO J. 4: 781- 785.
  8. Lee, J. H. 2002. Importance of nucleotide adjacent to the core region of Diphtheria tax promoter/operator. J. Microbial. Biotechnol. 12: 622- 627.
  9. Park, S. R., S. J. Cho, and H. D. Yun. 2000. Cloning and sequencing of pel gene responsible for CMCase activity from Erwinia chrysanthemi PY35. Biosci. Biotechnol. Biochem. 64: 925- 930.
  10. Park, S. R., M. K. Kim, J. O. Kim, D. W. Bae, S. J. Cho, Y. U. Cho, and H. D. Yun. 2000. Characterization of Erwinia chrysanthemi PY35 eel and pel gene existing in tandem and rapid identification of their gene products. Biochem. Biophys. Res. Commun. 268: 420- 425.
  11. Park, S. R., M. K. Kim, J. O. Kim, S. J. Cho, Y. U. Cho, and H. D. Yun. 2000. Cloning and sequencing of cel5Z gene from Erwinia chrysanthemi PY35. Mol. Cells 10: 269- 274.
  12. Park, Y. W., S. T. Lim, S. J. Cho, and H. D. Yun. 1997. Characterization of Erwinia carotovora subsp. carotovora LY34 endo-$\beta$- 1 ,4-glucanase genes and rapid identification of their gene products. Biochem. Biophys. Res. Commun. 241: 636- 641.
  13. Pissavin, C., J. Robert-Baudouy, and N. Hugouvieux-CottePattat. 1996. Regulation of pelZ, a gene of the peLBC cluster encoding a new pectate lyase in Erwinia chrysanthemi 3937. J. Bacteriol. 178: 7187-7196.
  14. Roeder, D. L. and A. Collmer. 1985. Marker-exchange mutagenesis of a pectate lyase isozyme gene in Erwinia chrysanthemi. J. Bacteriol. 164: 51- 56.
  15. Ruvkun, G. B. and F. M. Ausubel. 1981. A general method of site-directed mutagenesis in prokaryotes. Nature (London) 289: 85- 89.