• Korean Journal of Plant Resources
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• v.15 no.1
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• pp.18-25
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• 2002

These experiments were conducted to evaluate the variability of seed germination, and seedling growth of PEG priming barley. The average germination percentage(AGP) of PEG priming seed was higher than control, but those were low with extend the treatment period. The AGP of washing seed after PEG priming was higher than unwashed seed, and that of redried seed after priming was lower than control. The germination time of priming seed was short compare to control seed, and that was prolonged with extend the priming period, and that of washing seed after priming was shortening, and that of redried seed after priming was prolonging. The emergence percentage(EP) of priming seed was higher than control, and the emergence time was shortest in 50％ field moisture capacity soil. The seedling and root length of priming seed was shorter than control.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.4
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• pp.329-344
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• 1997

To improve germination and growth of forages of hill pasture seeds of several forages were osmotically primed with polyethylene glycol (PEG) under different PEG concentrations, treatment periods, and temperatures. Seeds were coated with several materials, and tested for germination. The best primed or coated seeds in germination tests were surface sow on hilly area. The results obtained are summarized as follows; 1. Osmotic priming with PEG accelerated germination of forages and reduced time taken for germination. 2. Germinability was best when the seeds of Tama ryegrass or orchardgrass were treated for 6-9 days at 30g PEG1100 ml water, red clover or white clover for 3, or 9 days in 20g PEG1100 ml water at $10^{\circ}C$. 3. Priming seeds of Tama ryegrass or orchardgrass in 30% PEG solution for 6 days, and seeds of red clover or white clover in 20% PEG solution for 6 days at $15^{\circ}C$ were most effective in germination, similar to priming at $10^{\circ}C$ . 4. Priming seeds of Tama ryegrass at 15C, and orchardgrass, red clover, or white clover at $10^{\circ}C$ were effective in germination than priming at other temperatures. 5. Osmotic priming with PEG accelerated germination of forages compared to coated seeds in Petri-dishes, while coated seeds germinated more slowly, but showed better emergence and superior growth to those of primed or intact seeds in the field. 6. Priming seeds increased yield slightly, and coating seeds significantly increased its yield on hilly area.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.1
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• pp.1-5
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• 1998

Experiments were carried out to find out the optimum water potential, temperature, and duration for the priming of rice seeds, Oryza sativa L. (cv. Ilpumbyeo) for better germination at sub-optimal temperatures. Seeds were primed in 0, -0.2, -0.4, -0.6, -0.8, and -1.0 MPa PEG (polyethylene glycol) solutions at $25^{\circ}C$. The optimum water potential for seed priming, the highest water potential at which rice seeds did not germinate, was -0.6 MPa. To find out optimum priming temperature and duration rice seeds were primed in -0.6 MPa PEG solution and 0 MPa (water as a control) for various durations at 15 and $25^{\circ}C$ and the seeds were germinated at 17, 20, and $25^{\circ}C$. Considering germination rate and speed, the optimum priming time in water (0 MPa) was 4 days at 15$^{\circ}C$ and 1 day at $25^{\circ}C$, while 4 days was the optimum priming time in a -0.6 MPa PEG solution, regardless of the priming temperature. Priming reduced the actual time of germination, especially at sub-optimal temperatures. Priming did not affect germination rate in -0.6 MPa PEG solution at 15$^{\circ}C$, but overpriming reduced the final germination rate in water at 15$^{\circ}C$ and in -0.6 PEG solution at $25^{\circ}C$. Total sugars and $\alpha$-amylase activity induced during the seed priming were negatively correlated with the final germination rate and there was no noted relationship with the speed or uniformity of germination.

• Horticultural Science & Technology
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• v.18 no.2
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• pp.98-102
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• 2000

This experiment was conducted to determine the effect of chemicals and their concentrations, priming temperature and duration, and different germination temperature on germinability of salvia seeds. The highest percentage of germination was obtained with 50 or 100 mM $KH_2PO_4$, or with -0.50 or -0.75 MPa PEG 8000. When number of days to attain 50% of the final germination percentage (T50) and mean number of days to germination (MDG) were taken into account, 50 mM $KH_2PO_4$ or -0.50 MPa PEG was most effective for early germination. No seeds germinated when primed in $K_3PO_4$ or NaOH solution. Priming the seeds at $20^{\circ}C$ was better than priming at $15^{\circ}C$ or $25^{\circ}C$. Priming at $20^{\circ}C$ for 4 or 6 days reduced the MDG by 2.3 days compared with nonprimed seeds. Seeds primed with -0.50 MPa PEG at $20^{\circ}C$ showed a high germination percentage with reduced T50 and MDG. When seeds were primed in a mixture of -0.50 MPa PEG and 50 mM $KH_2PO_4$ solution and germinated at $30^{\circ}C$ or $35^{\circ}C$, percent germination was lower than nonprimed seeds. However, the combined treatment retained the priming effect for reducing T50 and MDG.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.3
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• pp.263-266
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• 1999

Tobacco (Nicotiana tabacum L. ‘KF109’) seeds were primed in polyethylene glycol 6000 (PEG 6000) solutions to determine a) what osmotic potential of the solution would be optimal for priming, i.e., critical potential level for preventing germination, and b) what temperature and duration would be the most effective in priming. The germination was completely prevented below -0.8 MPa of PEG 6000, that indicates a optimum water potential for seed priming. Seeds were primed for 0, 1, 2, 3, 5, 10 and 15 days at 15, 20 and $25^{\circ}C$, respectively, under the-0.8 MPa PEG 6000 solution to find out the most effective temperature and duration for priming. The effectiveness of priming, particularly in germination speed, was observed more distinctly when the primed seeds were germinated at 15$^{\circ}C$ than 2 5$^{\circ}C$. The greatest reduction of the time to 50% germination (T/sob 50/) was when the seeds were primed at $25^{\circ}C$. The reduction rate of the $T_{50}$ was rapid when primed from 1 day to 8 days and then slowed down in the seeds primed for longer than 8 days. The time from 10 to 90% germination ( $T_{10-90}$ increased in the primed seeds for longer than 8 days which showed the reversed effect of synchronous germination. However, $T_{50}$ was reduced continuously in the seeds even primed over 8 days. Thus, the optimum condition for tobacco seeds priming with PEG 6000 solution was -0.8 MPa in osmotic potential of the solution at $25^{\circ}C$ for 8 days.ays.

• Proceedings of the Korean Society of Crop Science Conference
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• 1999.05a
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• pp.178-179
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• 1999

• Korean Journal of Medicinal Crop Science
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• v.26 no.1
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• pp.55-63
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• 2018

Background: The present study assessed the response of kenaf (Hibiscus cannabinus L., Jangdae) seed to NaCl and the effects of polyethylene glycol (PEG) on kenaf seed germination and vigor. Methods and Results: Seed germination ranged from 11.3% to 58.8% after 24 hours of immersion in NaCl concentrations from 0% to 0.5%. The priming treatments had lower electrical conductivity (EC) values for the seeds than for the control and a deteriorated palisade layer. Priming in 10% PEG for 48 hours increased the germination upto 96.3% in $H_2O$ solution and 98.8% in 0.3% NaCl solution compared to that of the control (78.8%). Germination synchronization, and shoot and root growth of the primed seeds were greater than those of the control. The $T_{50}$ of the control in $H_2O$ and 0.3% NaCl solution was 22 and 28 times, respectively. After priming, nine times was sufficient to reach $T_{50}$ in both solution. The mean number of days to germination (MDG) decreased from 1.43 days for the control to 0.55 days for 0% PEG in $H_2O$ solution and from 1.57 days for the control to 0.56 days for 0% PEG in 0.3% NaCl solution. The dry weight after the 10% PEG treatment was higher than that of the control. Conclusions: Taken together, 10% PEG treatment for 24 hours is recommended for kenaf seed invigoration before planting.

• Korean Journal of Plant Resources
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• v.19 no.4
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• pp.480-487
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• 2006

This study was conducted to improve the germination percent and germination speed by seed priming using polyethylene glycol (PEG) 8,000 solution on Acer mono seed because it has low and irregular germination characteristics. Seed priming was carried out under 0, -0.25, -0.5, -1.0 and -2.0 MPa of water potential at $20^{\circ}C$ for 3 days. Primed seeds showed higher percent germination, germination speed, mean germination time (MGT) and germination performance index (GPI) than unprimed seeds. Especially, primed seed with -2.0 MPa of water potential had the highest germination properties. And primed seeds had a lower dormancy than the unprimed seeds. Relative growth rate and T/R ratio of seedling from primed seeds were measured to survey the effect of seed priming on the seedling vigor as well as seed germination. The seedlings from primed seeds at -2.0 MPa of water potential showed the highest relative growth rates of height and root collar diameter, however, the lowest value in T/R ratio. This study suggested that seeds priming at -2.0 MPa of water potential under $20^{\circ}C$ for 3 days enhanced not only the percent germination, germination speed, MGT and GPI but also the vigor and growth of seedlings in Acer mono.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.4
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• pp.277-284
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• 2006

Seed priming is a useful technique for early establishment of seedling. In this experiment, the conditions for priming of pasture seeds (tall fescue, orchardgrass, alfalfa and white clover) have been optimized to ensure an early germination and more uniform growth of seedlings. The experiment was conducted in a split plot design with three replications. The main plots consisted of five different materials such as $KNO_3,\;KH_2PO_4,\;K_3PO_4,\;Ca(NO_3){_2}$ and PEG. The subplots were consisted of three chemical concentration groups such as 500mM (or 10%), 100mM (or 20%) and 200mM (or 30%). Effect of priming materials and its concentrations were different in all four pasture seeds examined. Chemical concentration did not show any significant effects on the germination of tall fescue, however, germination of tall fescue primed with $Ca(NO_3){_2}\;and\;K_3PO_4$ materials were higher than the others. The concentration of prime materials did not affect on the germination of orchardgrass, but germination of orchardgrass primed with $KH_2PO_4$ was showed better result than the other materials examined. The seeds of alfalfa and white clover primed with PEG showed better germination capability than the other priming materials. In addition, chemical concentration of primed seeds also showed significant difference on the germination of alfalfa and white clover. These results suggest that seed priming induced earlier and higher germination for all four pastures tested. However, the, degree of priming effectiveness on pasture species depending on the priming materials and its concentrations.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.4
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• pp.416-421
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• 2009

This experiment was conducted to find out optimum priming treatment conditions to the sesame seed as a preliminary study for enhancing sesame germination properties. Effective priming agents and concentrations for sesame seed were $K_3PO_4$, 200 mM and PEG6000, -1.0 MPa respectively. Optimum priming temperature and duration were $15^{\circ}C$, 4 days in view of germination speed and germination percentage. PEG6000 with -1.0 MPa was selected as an efficient priming treatment condition at $15^{\circ}C$, 4 days. This study suggested that priming treatment to sesame seed would be an effective technique enhancing sesame seed germination and shortening time to the $T_{50}$ at the field condition, but the efficiency of priming treatments to the sesame seed would be strongly dependent on individual or integrated conditions of priming agent, concentration, temperature and duration etc.