• Journal of the korean academy of Pediatric Dentistry
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• v.49 no.3
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• pp.321-328
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• 2022

The aim of this in-vitro study is to evaluate the effect of potassium iodide (KI) on erythrosine-mediated photodynamic therapy (PDT) against Streptococcus mutans biofilms. S. mutans ATCC 25175 was cultured to form a biofilm on a hydroxyapatite disk. After diluting erythrosine to 20 μM and KI to 10, 50, and 100 mM, respectively, PDT was performed. The number of surviving bacteria was calculated as colony forming units (CFU)/mL and the statistical significance of the difference between groups was confirmed by Bonferroni post-hoc analysis. Cell viability was visually evaluated using confocal laser scanning microscopy (CLSM). As a result of the experiment, a significant decrease (p < 0.05) in CFU was observed in the experimental groups in which PDT was performed after applying KI regardless of the concentration of KI. In addition, a significant reduction (p < 0.05) in CFU was observed in the experimental group to which 100 mM KI was applied compared to 10 mM KI. The same results were confirmed when observing CLSM. KI significantly improved the efficacy of erythrosine-mediated PDT on S. mutans biofilms at all concentrations. This may compensate for the low sensitivity of PDT to biofilm-state bacteria strains, but it is necessary to establish an optimal clinical protocol through further research.

• Tuberculosis and Respiratory Diseases
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• v.56 no.2
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• pp.178-186
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• 2004

Background : Photodynamic therapy (PDT) is a new therapeutic method aimed at the selective destruction of cancer cells. The outcome is death of cancer cells through apoptosis or necrosis. The aim of this study was to investigate the characterization of PDT induced cell death in A549 lung cancer cells. Materials and methods : A549 cells were used as the lung cancer cell. 5 aminolevulinic acid (ALA) was used as the photosensitizer and a 632nm diode laser (Biolitec, Germany) as the light source. Cells were incubated with various concentrations of ALA. The 632nm diode laser was then administered for various laser irradiation times. The treated cells were incubated with 24, 48 and 72 hours. The cell viabilities were measured using the crystal violet assay and light microscopy. To observe the cell death mechanism after PDT, cells were observed under fluorescence microscopy after double staining with Hoechst 33342 and propium iodide after PDT. Results : In the crystal violet assay at 24 hours after PDT with a $3.2J/cm^2$ laser irradiation power, the cell viabilities were $89.56{\pm}4.11$, $87.67{\pm}5.48$, and $69.37{\pm}8.84$ with ALA concentrations of 10, 100, and $1mg/m{\ell}$, respectively. In crystal violet assay at 24 hours after PDT with $1mg/m{\ell}$ of ALA, the cell viabilities were $74{\pm}19.85$, $55{\pm}6.1$, and $49.06{\pm}16.64%$ with 1.6, 3.2 and $6.4J/cm^2$ laser irradiation powers, respectively. However, increasing the interval time after PDT did not change the cell viabilities. In the apoptosis assay, photodynamic therapy was inducing the apoptotic cell death. Conclusions : This study shows the apoptotic anticancer effect of photodynamic therapy in A549 lung cancer cells. However, further evaluations with other cancer cells and photosensitizers are necessary.

• 대한두경부종양학회:학술대회논문집
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• 1998.05a
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• pp.123-124
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• 1998

• 대한두경부종양학회:학술대회논문집
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• 1998.05a
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• pp.121-122
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• 1998

• 종축개량
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• v.8 no.1
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• pp.10-10
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• 1986

• Geotechnical Engineering
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• v.23 no.3
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• pp.40-45
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• 2007

• Construction Engineering and Management
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• v.8 no.2
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• pp.24-26
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• 2007

• Journal of Photoscience
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• v.9 no.2
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• pp.506-508
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• 2002

Photodynamic therapy using topical 5-ALA has been used for non-melanoma skin cancers. Recently, the therapeutic method using incoherent light brought beneficial result in the treatment for mycosis fungoides. We used ALA-PDT for two Japanese patients suffering from lymphadenosis benigna cutis. In both cases, lesions were markedly faded and histologically, the number of infiltrated cells also decreased. We suggest that ALA-PDT can be used as an effective and safe modality in the treatment of benign cutaneous lymphoma.

• Journal of Advanced Marine Engineering and Technology
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• v.31 no.5
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• pp.522-530
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• 2007

In order to extract small hydropower in the very low specific speed range of $n_s<10$, a Positive Displacement Turbine (PDT) has been proposed and steady performances have been determined experimentally. However, the suppression of large pressure fluctuation is inevitable for practical application of PDT. Therefore, present study adopted 4-lobe helical type robe to reduce the pressure fluctuation. The results show that 4-lobe helical type robe can be adopted to suppress the pressure fluctuation drastically. Moreover, efficiency and unit power of the turbine with newly proposed 4-lobe helical type lobe are higher than those of the turbine with 3-lobe straight type robe.

• Proceedings of the Korea Multimedia Society Conference
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• 2003.05b
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• pp.366-369
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• 2003

본 연구에서는 강건한 문맥의존 음향모델을 작성하기 위한 기초적인 연구로서 문맥환경과 상태수의 변화에 따른 음향모델의 성능을 고찰하고자 한다. 음성은 시간함수로 표현되며 음절, 단어, 연속음성을 발성할때 자음과 모음에 따라 발성시간에 차이가 있으며 음성인식의 최소 인식단위로 널리 사용되는 음소의 앞과 뒤에 오는 문맥환경에 따라 인식성능에 많은 차이를 보이고 있다. 따라서 본 연구에서는 시간의 변화(상태수의 변화)와 상태분할 과정에서 문맥환경의 변화를 고려하여 다양한 형태의 문맥의존 음향모델을 작성하였다. 모델학습은 음소결정트리 기반 SSS 알고리즘(Phonetic Decision Tree-based Successive State Splitting： PDT-555)을 이용하였다 PDT-SSS 알고리즘은 미지의 문맥정보를 해결하기 위해 문맥방향과 시간방향으로 목표 상태수에 도달할 때까지 상태분할을 수행하여 모델을 작성하는 방법이다. 본 연구에서 강건한 문맥의존 음향모델을 학습하기 위한 방법의 유효성을 확인하기 위해 국어공학센터의 452 단어를 대상으로 음소와 단어인식 실험을 수행하였다. 실험결과, 음성의 시간변이에 따른 모델의 상태수와 각 음소의 문맥환경에 따라 인식성능의 변화를 고찰할 수 있었다. 따라서 본 연구는 향후 음성인식 시스템의 강건한 문맥의존 음향모델을 작성하는데 유효할 것으로 기대된다.