• BMB Reports
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• 제34권1호
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• pp.66-72
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• 2001

To investigate the mechanism of adriamycin (ADM) resistance in the ADM resistant subline PC-14/ADM, we examined the expressions of p-glycoprotein (P-gp), topoisomerase I (Topo I) and II (Topo II), glutathione-S-transferases (GSTs), tissue transglutaminase (t-TG), epidermal growth factor receptor (EGFR), and E-cadherin and the activity of superoxide dismutase (SOD) in PC-14 and PC-14/ADM cells. There was no change in the cellular levels of P-gp, Topo I, Topo II, and the two isoforms of GSTs. However, SOD activity in PC-14/ADM cells was 2.38 fold higher than that in PC-14 cells. A marked induction of the t-TG expression was also observed in PC-14/ADM cells. In addition to those changes, expressions of EGFR and E-cadherin were down regulated in PC-14/ADM cells. Therefore, molecular modifications such as an increase in SOD activity, induction of the t-TG expression, and down regulation of EGFR and E-cadherin expressions may play important roles in PC-14/ADM cells during the development of ADM resistance.

• 토지주택연구
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• 제14권1호
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• pp.115-122
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• 2023

최근 Covid-19 및 건설현장 인력 고령화 등으로 인하여 공동주택 생산성은 악화되고 있는 실정이다. 이에 탈현장(Off-Site Construction) 기반의 공장생산 방식으로의 공동주택 건설 방향 전환이 필요하다. 모듈러 공법은 공기단축이 가능하여 스마트 건축시스템으로 각광받고 있다. 이 연구에서는 모듈러 공법의 공기단축을 극대화 할 수 있는 PC코어 시스템을 개발하고, 구조성능을 해석적 연구를 통하여 검증하였다. 제안된 PC코어 시스템은 완전 PC형태로 이루어져 있으며, 접합방식은 건식 접합이다. 연구 결과, 제안된 PC코어 시스템은 모듈러 주택에 사용가능한 구조성능을 확보한 것으로 나타났다.

• 약학회지
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• 제43권3호
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• pp.369-377
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• 1999

A new series of highly water soluble platinum(II) complexes {Pt(II)[1,3-bis(phenylthio) propane](trans- -1,2-diaminocyclohexane) (PC-1) and Pt(II)[1,3-bis-(phenythio)propane] cis-1,2-diaminocyclohexane(PC-2)} were synthesized, and characterized by their elemental analysis and by various spectroscopic techniques[infrared(IR), 13C-nuclear magnetic resonance (NMR)]. In vitro antitumor activity of new Pt(II) complexes was tested against P-388 and L-1210 mouse lymphocytic leukemia cell lines, PC-14 / P, PC-14/ADM and PC-14 / CDDP human pulmonary adenocarcinima, DU-145 human prostate carcinoma, HT-1376 human bladder carcinoma, ZR-75-1 human breast carcinoma, MKN-45/P and MKN-45/CDDP human gastric adenocarcinoma cell lines using colorimetric MTT[3-(4,5-dimethyl thiazol-2-yl)-2.5-diphenyltetrazoliumbromide] assay for cell survival and proliferation. PC-1 showed active against L-1210, P-388 leukemia, human lung, stomach, prostate, bladder and breast cancer cell lines, and the antitumor activity of these compounds were comparable or superior to those of PC-2 and displatin. The nephrotoxicities of PC-1 and PC-2 were found quite less than that of cisplatin using MTT and [3H] thymidine uptake in rabbit proximal tubule cells and human kidney cortical cells. Based on these results, this novel platinum (II) complex compound (PC-1) represents a valuable lead in the development of a new anticancer chemotherapeutic agent capable of improving antitumor activity and low nephrotoxicity.

• Parasites, Hosts and Diseases
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• 제31권3호
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• pp.215-222
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• 1993

Toxoplasmngon gondii의 세포내 배양에 적합한 숙주 세포 주를 찾기 위하여 정상 세포 2종류(MDCK-canine kidney cells; Vero-monkey kidney cells) 및 암세포 6종류(A 549, PC 14-human lung cancer cells; SNU 1, SNU 16, MKN 45-human stomach cancer cells; HL-60-human promyelocytic leukemia cells)를 대상으로 하여 각 세포 주의 T.gondii 감염에 대한 감수성을 형태학적 관찰 및 3H-uracil 흡수 시험을 통하여 비교하였다. T.gondii 대한 감수성은 A 549 및 PC 14 세포가 가장 높았고, Vero, HL-60, MDCK 및 SNU 1 세포가 그 다음, SNU 16 및 MKN 45 세포는 가장 감수성이 낮았다. 또한 각 세포 주에 있어서 T.gondii 감염 후 충체증식 정도를 정량화하여 12시간, 36시간 및 60시간에 각각 측정한 바 충체 수를 적게($2{\times}10^5/ml$) 투여했을 때는 A 549, PC 14, Vero, MDCK 세포들에서 감염 60시간까지 충체의 분열 증식이 계속 증가하였고, 충체 수를 많이($50{\times}10^5/ml$) 주입하였을 때는 대부분의 세포들에서 감염 12시간에 최고의 증식을 보이다가 이후 증식이 감소하였다. 이상의 결과로 보아 기son사거 분리 계대 및 충주(strain) 확립을 위해서는 A 549 및 PC 14 세포가 가장 적합할 것으로 판단되며, 충체 주입 수 및 배양 시간별로 충체의 증식 정도가 다름을 알 수 있었다.

• 한국건축시공학회지
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• 제14권6호
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• pp.571-581
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• 2014

본 연구에서는 중공 PC 벽체 (PC Double Wall)를 적용한 계단실 공법을 개발하였다. 중공 PC 벽체는 내부 중공으로 인하여 양중 무게를 줄이고 외부 벽체가 거푸집 역할을 하며, 벽체 내부 중공에 현장 콘크리트를 타설하여 기존 PC 벽체와 비교하여 부재간의 일체성 확보가 우수하다. PC Double Wall 제작 및 현장 콘크리트 타설, 구조적 안전성을 고려하여 PC 벽체 단면과 철근 배근 상세를 제안하였다. 제안 상세를 바탕으로 얇은 PC벽체와 중공부를 효과적으로 형성하기 위한 세움 타설 방식의 거푸집 시스템을 개발하였다. PC Double Wall을 이용하여 계단실 Mock-up test를 통하여 부재 간 일체성과 시공성을 검증하였으며, 부재 간 접합부의 변형 및 균열이 발생하지 않고 시공성이 우수한 것으로 나타났다.

• Korean Journal of Acupuncture
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• 제21권2호
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• pp.177-192
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• 2004

Objectives : This study is designed to investigate the pathology, acupuncture and moxibustion therapy applicable of the dementia through literature of oriental medicine. Results : The findings of this study are as follows ; 1. The cause of dementia is internal damage of seven emotions(七情內傷), deficiency of the kidney essence(腎精不足), retention of dampness in the mental(痰迷心竅). 2. The acupuncture meridians used often for dementia are $Tok-maek(GV),\;Chok-t'aeyang\;Pang-gwang-Ky\check{o}ng(BL),\;Chok-yangm\check{o}yng\;Wi-Ky\check{o}ng(ST),\;Su-gwor\check{u}m\;Shimp'o-Ky\check{o}ng(PC),\;Su-so\check{u}m\;Shim-Ky\check{o}ng(HT)$. 3. The spots on the body suiTable for acupuncture used for therapy of the dementia are 98 points and extra points are 6 points, total therapeutic acupunture points are 104 points. And acupunture points are frequently used Shinmun(神門-HT7, 24), Paek'oe(百會-GV20, 19), Chok-samni(足三里-ST36, 18), Sugu(水溝-GV26, 17), P'ungnyung(豊隆-ST40, 16), Taech'u(大椎-GV14, 14), $Sam\check{u}mgyo(三陰交-SP6,\;14)$, Shimsu(心兪-BL15, 14), $Taen\check{u}ng(大陵-PC7,\;11)$, Nogung(勞宮-PC8, 11), Sosang(少商-LU11, 10), Kansa(間使-PC5, 10), Naegwan(內關-PC6, 10). 4. The acupoints used often on moxibustion are Paek'oe(百會-GV20), Chang-gang(長强-GV1), Taech'u(大椎-GV14), Kansa(間使-PC5). Conclusion : It is recommended that further study of many sided investigations in the future.

• 전자진흥
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• 제26권6호
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• pp.10-14
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• 2006

유비쿼터스 환경과 맞물려 새로운 성장 동력으로 떠오르고 있는 차세대 PC 분야의 동향과 비전을 점검해본다.

• 한국지진공학회논문집
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• 제14권2호
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• pp.15-26
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• 2010

PC(Precast Concrete) 구조시스템은 건식화 조립식 공법으로 구조물 건설에 있어 공기, 노동력 절감 등의 여러 장점을 가지고 있다. 하지만 구조벽체의 경우 이를 PC화하여 사용할 경우 접합부의 내진성능이 떨어지므로 PC 구조벽체를 사용하는 경우는 드물다고 할 수 있다. 본 연구에서는 이러한 점을 감안하여 접합부 성능을 개선하여 횡력저항요소로 사용가능한 2종류의 PC 구조벽체를 제안하였다. 제안된 PC 구조벽체는 RC 벽체 및 PC 벽체가 혼합된 복합 PC 벽체와 벽체하부 연결철근의 일부를 단면감소시키고 비부착상세를 적용한 PC 벽체로서 지진발생 시 충분한 강도와 변형능력을 확보할 수 있도록 하였다. 제안된 PC 구조벽체의 내진성능평가를 위하여 RC 벽체를 포함한 3가지 벽체에 대한 실험체를 제작하여 주기 횡하중 실험을 수행하였다. 실험결과, 제안된 PC 구조벽체는 강도, 강성, 변형능력 및 에너지 소산능력이 기존 PC 구조벽체보다 월등히 향상되어 우수한 내진성능을 확보할 수 있는 것으로 나타났다.

• 한국IT서비스학회지
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• 제14권4호
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• pp.185-197
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• 2015

As the uncertainty of demand in B2B electronics market has increased, firms need a strong method to estimate the market demand. An accurate prediction on the market demand is crucial for a firm not to overproduce or underproduce its goods, which would influence the performance of the firm. However, it is complicated to estimate the demand in a B2B market, particularly for the private sector, because firms are very diverse in terms of size, industry, and types of business. This study proposes both qualitative and quantitative demand forecasting approaches for B2B PC products. Four different measures for predicting PC products in B2B market with consideration of the different PC uses-personal work, common work, promotion, and welfare-are developed as the qualitative model's input variables. These measures are verified by survey data collected from experts in 139 firms, and can be applied when individual firms estimate the demand of PC goods in a B2B market. As the quantitative approach, the multiple regression model is proposed and it includes variables of region, type of industry, and size of the firm. The regression model can be applied when the aggregated demand for overall domestic PC market needs to be estimated.

• 한국식품영양과학회:학술대회논문집
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• 한국식품영양과학회 2001년도 International Symposium on Food,Nutrition and Health for 21st Century
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• pp.74-87
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• 2001

Evidence shows that the serum level of cholesterol (CH) is decreased with increasing green tea (GT) consumption. This presentation summarizes our recent findings on the effect of GT extract on intestinal absorption of [$^{14}C$-labeled CH and phosphatidylcholine (PC). Ovariectomized (OX) adult rats were infused intraduodenally with lipid emulsions containing radiolabeled lipids [$^{14}C$-CH or $^{14}C$-phosphatidylcholine (PC)] in the presence of GT extract or catechins to determine the rates and amounts of CH absorption and the intestinal hydrolysis and lymphatic output of PC. During lipid infusion, lymph was collected hourly for 8 h. The lymphatic absorption of $^14C$-CH was drastically lowered by infusion of GT extract at two dosage levels (GTl =5.4 mg catechins/h and GT2 = 15.1mg catechins/h). The cumulative lymphatic absorptions of $^{14}C$-CH in rats infused with GT1 and GT2 were 20.7$\pm$4.3 and $4.8{\pm}4.1{\%}$ dose, respectively, whereas the absorption of $^{14}C$-CH in rats infused with no GT extract (GT0) was $36.3{\pm}1.1{\%}$ dose. GT extracts also significantly lowered the absorption of-tocopherol (TP) in a dose dependent manner ($29.6{\pm}4.9{\%}$ dose in GT0, $20.8{\pm}5.8{\%}$ dose in GTl, and $7.9{\pm}5.4{\%}$ dose in GT2 groups). Both (+)-catechin and EGCG significantly lowered the lymphatic outputs of $^{14}C$-radioactivity after intraduodenal $^{14}C$-PC infusion. A significantly higher amount of $^{14}C$-PC remained unhydrolyzed in the intestinal lumen of the EGCG rats ($22.8{\%}$) compared with the (+)-catechin ($15.8\%$) and control groups ($11.9\%$). GT extracts, (+)-catechin, and EGCG significantly reduced the absorption of TP. The inhibitory effect of GT extract and catechins on lipid absorption may be mediated in part through the inhibition of pancreatic PLAz. The findings provide the first direct evidence that green tea and catechins have a profound inhibitory effect on the intestinal absorption of CH in OX rats. Results suggest that green tea and catechins may be used as a dietary or pharmacological means of lowering cholesterol absorption.