• 대한본초학회지
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• 제35권3호
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• pp.25-32
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• 2020

Objectives : At present, aging-related degenerative muscle diseases are considered a serious problem. However, the effects on muscles regarding the efficacy of blueberry have not been studied. In this study, we tried to find out the correlation between blueberry and muscle. Methods : 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay was performed to confirm the antioxidant efficacy of blueberry hydrothermal extract. To determine the effect of blueberry hydrothermal extracts (BHE) on myoblast activity, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was performed. To confirm the effect of blueberry hydrothermal extracts on the differentiation of myoblast into myotubes, protein expression levels of myosin heavy chain 3 (Myh3) and paired box 3/7 (pax3/7) were confirmed by immunoblot analysis. In addition, immunofluorescence microscopy was performed to confirm the effect on myotube formation of blueberry hydrothermal extracts. Results : Antioxidative efficacy and low toxicity were confirmed through ABTS assay and MTS assay of blueberry extract for myoblasts. As a result of immunoblot analysis and immunofluorescence analysis, the decrease in myogenic marker Pax3/7 was not confirmed, but myotubes The specific expression inhibitory activity of the forming protein Myh3 was confirmed. Through this, it was confirmed that the blueberry extract has a negative activity against myoblast differentiation. Conclusion : This experiment confirmed that blueberry hydrothermal extract has excellent antioxidant efficacy and negative results in inhibiting the differentiation and proliferation of myoblast. This requires deep study of certain ingredients and requires reassessment of the dietary intake of blueberries.

• Childhood Kidney Diseases
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• 제26권1호
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• pp.40-45
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• 2022

Purpose: Chronic kidney disease (CKD) has various underlying causes in children. Identification of the underlying causes of CKD is important. Genetic causes comprise a significant proportion of pediatric CKD cases. Methods: In this study, we performed whole-exome sequencing (WES) to identify genetic causes of pediatric CKD. From January to June 2021, WES was performed using samples from pediatric patients with CKD of unclear etiology. Results: Genetic causes were investigated using WES in 37 patients (17 males) with pediatric CKD stages 1 (n=5), 2 (n=7), 3 (n=2), 4 (n=2), and 5 (n=21). The underlying diseases were focal segmental glomerulosclerosis (n=9), congenital anomalies of the kidney and urinary tract including reflux nephropathy (n=8), other glomerulopathies (n=7), unknown etiology (n=6), and others (n=7). WES identified genetic causes of CKD in 12 of the 37 patients (32.4%). Genetic defects were discovered in the COL4A4 (n=2), WT1 (n=2), ACTN4, CEP290, COL4A3, CUBN, GATA3, LAMA5, NUP107, and PAX2 genes. WT1 defects were found in patients whose pathologic diagnosis was membranoproliferative glomerulonephritis, and identification of CUBN defects led to discontinuation of immunosuppressive agents. Genetic diagnosis confirmed the clinical diagnosis of hypoparathyroidism, sensorineural deafness, and renal disease; Alport syndrome; and Joubert syndrome in three of the patients with CKD of unknown etiology (COL4A4 [n=2], CUBN [n=1]). Extrarenal symptoms were considered phenotypic presentations of WT1, PAX2, and CEP290 defects. Conclusions: WES provided a genetic diagnosis that confirmed the clinical diagnosis in a significant proportion (32.4%) of patients with pediatric CKD.

• 한국동물생명공학회지
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• 제38권3호
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• pp.177-187
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• 2023

Background: The grading of Hanwoo (Korean native cattle) is based on four economic traits, and efforts have been continuously made to improve the genetic traits associated with these traits. There is a technology to predict the expected grade based on the 4 economic genetic SNP characteristics of Korean cattle calves using single nucleotide polymorphism (SNP) technology. Selection of highly proliferative, self-renewing, and differentiating satellite cells from cattle is a key technology in the cultured meat industry. Methods: We selected the Hanwoo with high and low-scored of genomic estimated breeding value (GEBV) by using the Hanwoo 50K SNP bead chip. We then isolated the bovine satellite cells from the chuck mass. We then conducted comparative analyses of cell proliferation, immunocytochemistry, qRT-PCR at short- and long-term culture. We also analyzed the differentiation capability at short term culture. Results: Our result showed that the proliferation was significantly high at High scored GEBV (Hs-GEBV) compared to Low scored GEBV (Ls-GEBV) at short- and long-term culture. The expression levels of Pax3 were significantly higher in Hs-GEBV bovine satellite cells at long-term culture. However, there were no significant differences in the expression levels of Pax7 between Hs- and Ls-GEBV bovine satellite cells at short- and long- term culture. The expression levels of MyoG and MyHC were significantly high at Ls-GEBV bovine satellite cells. Conclusions: Our results indicated that selection of bovine satellite cells by Hanwoo 50K SNP bead chip could be effective selection methods for massive producing of satellite cells.

• 한국해양공학회:학술대회논문집
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• 한국해양공학회 2004년도 학술대회지
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• pp.290-297
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• 2004

In this paper, one of the harbour maneuvering tests is described. Its goal is to investigate the so-called crabbing performance of ships. By crabbing is meant the ability of the vessel to move sideways with the use of her own maneuvering devices like propellers, rudders, transverse thruster, etc. the 3m ferry model is made by ocean engineering wide tank in the university of ulsan. when the model ship is leaving and going to the quay, Transverse forces and Yawing moments are measured, in the condition of zero longitudinal force and the design draught, by load cell attached to the longitudinal center of gravity on the ship. the purpose of the experiment is to verify the reliability of ocean wide tank in university of ulsan about crabbing test compared with results of foreign research institute.

• 대한조선학회 특별논문집
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• 대한조선학회 2017년도 특별논문집
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• pp.63-66
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• 2017

All passenger ships aboard more than 36 passengers are to be able to easily find escape routes when the escape way by fire with flame spread were onboard vessel (incl. Ferry, Passenger, Ro-pax, Inland Ferry) It should be impossible by ordinary emergency lights. The international Maritime Organization (IMO) gives special requirement for luminescent evaluation onboard test procedure and applicable location of low location lighting. In order to maintain the residual light source for a certain time in the low location lighting with a strip-shaped light source, the light source material must be sufficiently exposed to the light due to the proper placement of the escape route. The lighting arrangement influence how maintain low location lighting strip luminescent which measured onboard in connection with what elements are mainly sustain luminescent.

• Natural Product Sciences
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• 제12권3호
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• pp.125-128
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• 2006

A chemical examination of the stems of Sapium japonicum PAX et $H_{OFFM}$ (Euphorbiaceae) has led to the isolation of seven phenolic compounds. On the basis of UV, IR, MS, and NMR spectral data and the chemical reaction, the structures of these compounds were identified as gallic acid (1), ellagic acid (2), 3,3'-di-O-methylellagic acid (3), $4-O-({\beta}-D-xylopyranosyl)-3,3'-di-O-methylellagic$ acid (4), $4-O-({\alpha}-D-arabinofuranosyl)-3,3'-di-O-methylellagic$ acid (5), isoquercitrin (6), and geraniin (7).

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 심포지엄 논문집 정보 및 제어부문
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• pp.166-168
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• 2007

This paper is dealing with how to control of a client robot's movement for instructions from a server PC and a wireless andremote control Robot that sends the server information of images for monitoring. To implement this. 802.11x WLAN with TCP/IP socket programming is used to get the driving instructions from the server PC and control movements of the robot such as a forward, backward and directions. As well as this, ARM9 cored PAX255 embedded processor and Linux OS is used for the function transmitting BMP format of 320 ${\times}$ 240 pixel for stopped image data.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2019년도 추계학술대회
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• pp.127-129
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• 2019

전라남도 완도 연안여객선 터미널에서 2018년 10월 연안여객선 현대화편드 1호에 의한 선박 '한일고속페리 실버클라우드호'가 취항식을 가졌다. 국내 여객선 시장은 그간 해외 중고선에만 의존해왔는데 순수 국내 기술로 건조된 여객선이 등장한 것이다. 이에 따라 우리나라 여객선 산업은 기술발전과 디자인 등 그 품질이 향상 될 것이다. 그간 여객선 이용객의 수요는 꾸준히 증가하고 있으나 영세한 규모의 국내 선사는 비용 부담 때문에 신조를 건조하기 어려워 해외 중고선을 수입하였다. 이로 인해 국내 여객선의 실내 인테리어의 노후화, 인테리어 개조에 따르는 선박 성능의 저하 등 다양한 문제점을 가지고 있었다. 따라서 정부는 2018년 현대화펀드를 통해 노후된 연안여객선을 대체할 새로운 선박의 건조를 지원함으로써 연안여객선은 이제 선사의 사적 소유물을 넘어 공공적 성격이 교통수단이 되었다. 이로써 연안여객선은 이동 편의 향상을 비롯하여 승객에게 더 큰 만족을 줄 수 있는 디자인 개선방안이 요구된다.

• 대한조선학회 특별논문집
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• 대한조선학회 2007년도 특별논문집
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• pp.68-73
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• 2007

CPP is widely utilized in RoPax ship, shuttle tanker etc. due to excellent manoeuvrability in low speed, and its usage is recently increased. The CPP was almost designed by CPP maker, and its performance seemed to be not fully optimized. In this study the whole CPP design procedure was reviewed and design technology of CPP was settled down including calculation of spindle torque and MOI, which was fully confirmed by KHI CPP maker. In order to confirm the CPP design technique, a CPP for shuttle tanker was designed and its performance was verified through series of model tests. The propeller efficiency and face cavitation performance of the CPP was well improved. This CPP design technology will be contributed to the optimization of performance and cooperation with CPP maker.

• Journal of Plant Biology
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• 제27권3호
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• pp.129-138
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• 1984

Floral structures of 11 korean lauraceous species belonging to six genera have been studied. Machilus is a primitive genus having relatively large bisexual flower, six tepals and staminodes in fourth whorl of stamen. The flowers of Neolitsea are highly reduced state as unisexual flower, six stamens arranged to three whorls and no staminode in the flower, so regared as more advanced group. Intrafamilial classification systems of Nakai (1939) and Pax (1891) are more reasonable but still have some discrepancies with results. The origin of tepals of this group has been a long controversial problem; whether the differentiation of tepals occur. Teratological study of Lindera erythrocarpa, inner tepals of this group must be originated from the staminode and outer tepals from leafy tepals. Although morphological differentiation does not sitll occur, inner and outer whorls of tepals are originated through different way.