• 한국어병학회지
• /
• 제21권1호
• /
• pp.45-56
• /
• 2008

This study was conducted to find out biological responses of bivalves exposed to organotin compound.The results of the study confirmed that tribultyltin chloride (TBTCl) induce reduction of survival rate andburrowing activity, and histopathological feature in the foot structure of the equilateral venus, Gomphinaveneriformis. The experimental period was 36 weeks. The experimental groups consisted of a control and 3TBTCl exposure groups (0.4, 0.6, 0.8 ym TBTCl L'). The survival rate and burrowing activity were record-ed daily. For histological analysis, foot tissues were fixed in Bouin' s fluid and then stained H-E stain, AB-PAS (PH 2.5) reaction and Masson's trichrome stain after having serially sectioned the tissue by paraffinmethod at thickness of 4-6 ym. The survival rate was not significantly different between the control andexposure groups for 20 weeks, but in 0.8 Um TBTCl L', it was on the decreased ever since the exposure. Theburrowing activity was not significantly different in the exposure group compared to the control up to 12weeks, but in 0.6 and 0.8 ym TBTCl L', it measured the lowest level after 20 weeks. The foot is composedof the epidermal layer, connective tissue, and muscular layer. The epidermal layer is composed of simplecolumnar, cuboidal epithelia and mucous cells. The cilia were well developed on the apical surface ofepithelium, Circular, longitudinal and transverse muscle bundle were well developed in the muscular layer.The majority mucous cells showed blue color (542c) when it subjected to AB-PAS (PH 2.5) reaction. Nohistopathological alterations in the foot were observed up to 12 weeks. After 20 weeks of exposure to 0.8 (anTBTCl L'', the foot samples of exposed G. veneriformis showed disappearance of cilia and striated borderpartially and extension of hemolymph sinus. The mucous cell increased in the marginal of foot. At 28-weekof exposure to 0.4 ym TBTCl L', it observed weekly acid (564c), neutral (264c) and mixed mucous cell. At36-week of exposure to 0.6 ym TBTCl L', it showed fragmentation of the muscle and collagen fiber bundle,and also diappearance of cilia on epithelia and edema of epithelium in 0.8 ym TBTCl L''.

• Journal of Life Science
• /
• 제10권2호
• /
• pp.1-8
• /
• 2000

This experiment was performed to study the structure of esophageal mucousa and the histochemical properties of glycosaminoglycans of esophageal mucous cells in four teleostean species, i.e., Agramus agramus, Inimicus japonicus, Epinephelus chlorostigma and Helicolenus dactylopterus. To observe the structure of eosphageal mucosa, hematoxylin-eosin(H-E) staining was used. The glycosaminoglycans was stained with PAS(periodic acid schiff), alcain blue(AB) pH 2.5, AB pH 1.0, aldehyde fuchsin(AF) pH 1.7, AF pH 1.0, AB pH 2.5-PAS, AB pH 1.0-PAS and AF pH 1.7-alcian blue pH 2.5. As for the amount and histochemical composition of glycosaminglycans in Agramus agramus, most of mucous secreting columnar cell and mucous cells contain large and moderate amount of neutral glycosaminoglycans. A few of mucous cells having small amount of neutral glycosamino-glycans and minimal amount of sulfated glycosamino-glycans. In Inimicus japonicus, the esophageal mucous cells were composed of most of medium sized and large mucous cells with moderate amount of neutral glycosaminoglycanonly only, a few of medium sized and large mucous cells and most of small mucous cells with considerable amount of neutral glycosaminoglycans and minimal to small amount of nonsulfated glycosaminoglycans, and a feq of small mucous cells with small amount of neutral glycosaminoglycans and minimal amount of sulfated glycosaminoglycans. In Epinephelus chlorostigma, most of medium sized and large mucous cells were mixed small amount of neutral glycosaminoglycans with sulfated glycosaminoglycans, a few of which were contained with moderate or considerable amount of neutral glycosaminoglycans with sulfated glycosaminoglycans, while most of small mucous cells containing considerable amount of neutral glycosaminoglycans and small to moderate or considerable to minimal amount of nonsulfated glycosaminoglycans(sialomucin) a few of which containing a mixture of considerable amount of neutral glycosaminoglycans and considerable amount of nonsulfated glycosaminoglycans(sialomucin)only. In Helicolenus dactylopterus, most of medium sized and large mucous cells, mixing with moderate to considerable amount of neutral glycosaminoglycans and small to moderate amount of sulfated glycosaminoglycans, while most of small mucous cells with considerable amount of nonsulfated glycosaminoglycans (sialomucin), a few of which having a mixture of considerable amount of neutral glycosaminoglycans and considerable or small amount of nonsulfated glycosaminoglycans(sialomucin).

• Journal of Preventive Medicine and Public Health
• /
• 제11권1호
• /
• pp.14-23
• /
• 1978

This survey was conducted on a total 672 pulmonary tuberculosis patients who were registered at certain health center in Busan, during the period from July 15th to August 31st, 1977, based on Modified Cornell Medical Index(CMI) consisting of 70 questions. Number of 'Yes' response of an individual patients was collected by each large section of Modified CMI. The each number of 'Yes' response was standardized by mean of Z scoring. Z score was obtained by following formula. Z=50+10(Xi-m)/s M : means of 'Yes' response by each section for all subjects s : standard deviation of the mean Xi : number of 'Yes' response by each section in an individual patients The results of obtained were as follows: 1. The number of investigated cases were 672 (459 males and 213 females). The most prevalent group was 20-24 years old group as 18.4% by age, moderate advanced group as 50.8% by radiological diagnosis, INH+PAS+SM group as 34.7% by antituberculotics and unemployed group as 59.9% by occupation. By bacteriological examination of sputum, the rate of negative group was 60.5% and positive group was 39.5%. 2. Z score of complaints by sex was higher in female as 52.4 than in male as 48.9 in general. By radiological diagnosis, there was decreasing tendency with age in male but increasing tendency with age in female. 3. By age group, Z score of complaints was increasing tendency with age in male but there was non-significant differences in female. 4. By bacteriological examination of sputum, the Z score of complaints was increasing tendency with the more discharged bacteria in both sex generally. 5. By antituberculotics, INH group was revealed the highest Z score of complaints as 50.4 in male and INH+PAS group was the highest as 51.21 in female. 6. By occupation, agricultural and fisherman group was the highest as 53.5 and the next group was professional, technical and related workers, unemployed and sales workers in that order.

• Tuberculosis and Respiratory Diseases
• /
• 제58권3호
• /
• pp.213-229
• /
• 2005

The first and the longest criminal indictment case of Korean medico-legal battle, so called BORAMAE Hospital Incident, was finally on its end by Korean Supreme Court's decision on June 24, 2004, after 7 years long legal dispute via Seoul District Court and Seoul Superior Appeal Court's decision. Boramae Hospital case was the first Korean legal case of Withdrawing Life-sustaining treatment of mechanical respirator on 58 years old Extradural Hematoma victim who was on Respirator under Coma after multi-organ failure postoperatively(APACHE II score: 34-39). Two physicians who have involved patient's care and had helped to make discharge the Near-death patient to home after repeated demand of patient's wife, due to economic reason, were sentenced as homicidal crime. This review article will discuss the following items with the review of US cases, Quinlan(1976), Nancy Cruzan(1990), Barber (1983), Helen Wanglie(1990), Baby K (1994) and Baby L cases, along with Official Statement of ATS and other Academic dignitaries of US and World.: [1] Details of Boramae Hospital incident, medical facts description and legal language of homicidal crime sentence. [2] The medical dispute about the legal misinterpretation of patient's clinical status, regarding the severity of the victim with multi-organs failure on Respirator under coma with least chance of recovery, less than 10% probability. [3] Case study of US, of similar situation. [4] Introduction of ATS official Statement on Withdrawing/ Withholding Life sustaining treatment. [5] Patient Autonomy as basic principle. [6] The procedural formality in Medical practise for keeping the legitimacy. [7] The definition of Medical Futility and its dispute. [8] Dying in Dignity and PAS(Physician Assisted Suicide)/and/or Euthanasia [9] The Korean version of "Dying in Dignity", based on the Supreme Court's decision of Boramae Hospital incident (2004.6.24.) [10] Summary and Author's Note for future prospects.

• 한국산학기술학회논문지
• /
• 제14권2호
• /
• pp.721-727
• /
• 2013

본 연구는 모음에 따라 발성역치압력과 비성도의 차이를 비교하고자 하였다. 2012년 5월부터 8월 동안에 대학에 재학 중인 20~30대의 정상성인 여성 28명을 대상으로 실시하였다. 발성역치압력은 객관적인 공기역학적 검사도구인 PAS(Phonatory aerodynamic system, model 6600, KAY electronics, Inc)를 이용하였으며, /p/와 세가지 모음 /a, i, u/를 각 5번씩 측정하여 가운데 3개를 비교하여 분석하였다. 또한 비성도는 Nasometer${\Pi}$(model 6450, KAY electronics, Inc)를 이용하였으며, 세가지 모음 /a, i, u/를 5초 이상 연장발성하여 측정하였다. 그 결과, 모음에 따른 발성역치압력은 유의한 차이를 나타내었으며, 모음에 따른 비성도도 유의한 차이를 보였다. 발성역치압력과 비성도간의 상관관계도 매우 높은 상관성을 나타내었다. 따라서 모음간의 발성역치압력은 연인두 폐쇄정도를 예측할 수 있으며 그에 따른 비성도와 매우 연관성이 깊음을 알 수 있었다.

• 대한임상검사과학회지
• /
• 제39권2호
• /
• pp.122-127
• /
• 2007

This study is a report about a specific patient whose primary stomach adenocarcinoma metastasized to uterine cervix adenocarcinoma. A thirty-nine year old female patient was initially diagnosed as having metastatic adenocarcinoma in the supraclavicular lymph node. Upon further examination, she was diagnosed with stomach adenocarcinoma. 8 months later, a cervix punch biopsy was performed. The stains used for examination were H&E stain, PAS stain, Alcian blue stain, Mucicarmine stain, Papanicolaou's (Pap.) stain, and as immunohistochemical stains, cytokeratin 7 and 20 were done. In the H&E stain, the tumor cells showed prominent and eccentric nuclei, thin nuclear membrane in abundant mucous cytoplasm, and cylinder shape. In the PAS stain, intracytoplasmic mucin vacuoles were stained with pink, and in Alcian blue and Mucicarmine stains, intracytoplasmic mucin vacuoles were stained with blue and red. As in the above results, she was diagnosed with undifferentiated adenocarcinoma. As found on the cytologic smear preparation of the uterine cervix stained by Papanicolaou's stains, the background was relatively clear, the number of malignant cells was relatively low, and large and eccentric nuclei in abundant cytoplasm were observed. Upon observing the tissue preparation of the uterine cervix biopsy by H&E stain, a clear background, large and eccentric nuclei, and a signet ring cell types were observed, and the number of malignant cells were fewer than in the primary uterine cervix adenocarcinoma. The vacuoles in cytoplasm were observed. The nuclear membrane and chromatin were thick and very rough, and upon observation by cytokeratin 7 and 20 of immunohistochemical stain, the tumor cells indicated a positive rate of 70% and 20%, respectively. According to these results, also she was diagnosed with metastasized uterine cervix adenocarcinoma. In summary of the results of pathologic findings on stomach biopsy and cytologic, histopathologic, and immunohistochemical finding on uterine cervix biopsy, the adenocarcinoma of her uterine cervix could assert the adenocarcinoma of signet ring cell type that was metastasized from the primary undifferentiated adenocarcinoma in stomach.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제29권3호
• /
• pp.173-181
• /
• 2003

Purpose : This study was intended to evaluate the positional relationship between the hyoid bone and the mandible in patients with mandibular protrusion after mandibular set-back surgery by means of 3D-CT. Materials and methods : Preoperative(3 weeks before) and postoperative (6 weeks after) 3D-CT & cephalogram were taken on 32 patients(12 male, 20 female, mean age of 23.2) treated by bilateral sagittal split osteotomy with rigid fixation. The angular measurement on 3D-CT basilar view were deviation of Me & H, long axis angle of left & right cornu majus. The lineal measurement on 3D-CT basilar view were composed of intercondylar line and coordinates(x,y) of Me & H. The angular & lineal measurement of lateral cephalogram were composed of mandibular plane angle, SNA, SNB, ANB, FH-NA & FH-NB, and coordinates(x,y) of B, Pog, Me & H, PAS, Lpw, MPH and IAS. On the frontal cephalogram, deviation of Me were evaluated. Results : The mean mandibular set-back was 8.0mm horizontally and mandibular plane angle was slightly increased. The hyoid bone was displaced postero-inferiorly, the distance between MP(mandibular plane) and H(hyoid bone) was increased and the posterior airway space values (PAS, Lpw, IAS) were decreased. The coordinates Me(x,y), H(x,y) and deviation angle Me'& H' were revealed the strong positive correlation. Conclusion : The results revealed that the horizontal, vertical and transverse relationship of the mandibular and the hyoid bone movements were significantly correlated in patients performed mandibular set-back surgery.

• 한국환경과학회지
• /
• 제8권6호
• /
• pp.711-716
• /
• 1999

This research explored the feasibility of preparing and utilizing a prefonned polymeric solution of Al(III) for coagulation in water treatment. Slow base(NaOH) injection into supersaturated aluminum chloride and aluminum sulfate solutions did produce high yields of Al polymers useful to water treatment applications. The method of characterization analysis was based on timed spectrophotometer with ferron as a color developing reagent. The hydrolytic Al species were divided into $monomeric(Al_a),\;polymeric(Al_b),\;and\;precipitate(Al_c)$ from the difference in reaction kinetics. The analysis of PACl's characteristics showed that the quantity of polymeric Al produced at value of$ r(OH_{added}/AI)=2.2$ was $83\%$ of the total aluminum in solution, as showing maximum contents and precipitated Al was dramatically increased when r was increased above 2.35. In addition, the characteristics of polyaluminum sulfate (PAS) showed that polymeric Al contained at r = 0.75 was $18\%$ of the total aluminum in solution. The synthesized PACI and PAS were stable during storing period, as indicating negligible aging effect. The effect of sulfate ion on PACI was dependent on the concentration of sulfate ion. That is, polymeric species decrease and precipitate species increase as sulfate ion concentration increased. It can be concluded that the sulfate cause the formation of $Al(OH)_{3(S)}$ at low pH. However, The effect of calcium ion was negligible for distribution of Al species.

• 한국식품영양과학회지
• /
• 제33권2호
• /
• pp.318-323
• /
• 2004

갈근(Radix puerariae, 15%)과 죽력 ( Cause in Liquamen Phyllostachyos, 1%)을 함유한 음료가 알코올을 투여한 생쥐에 미치는 영향을 구명 하고자 하였다. 대조군은 생쥐에 대조군은 생쥐에 알코올(40% alcohol, 8.0 mL/kg)을 단독 투여 하였으며, 실험 1군은 숙취 해소음료(8.0 mL/kg)를 투여한 다음 알코올을 투여하였고, 실험 2군은 알코올을 투여한 다음 동일음료를 투여하였다 실험 1군과 실험 2군의 알코올 농도는 대조군에 비하여 각각 유의성 있게 감소하였다. ALT활성 변화는 없었으나, AST의 활성은 실험 1군과 실험 2군에서 대조군에 비하여 다소 증가하였다. Mn-SOD의 활성은 대조군에 비하여 실험군에서 유의성 있게 증가하였다. 간조직의 PAS 반응은 대조군에 비하여 실험 1군과 실험 2군에서 높게 나타났다. 전자현미경을 통한 미세구조적 관찰 결과 실험 1군과 실험 2군에 비하여 대조군에서 세포소기관의 손상정도가 심하였다. 이상의 결과로 보아 갈근과 죽력을 함유한 기능성 음료는 숙취해소에 안전하고 효과적으로 사용할 수 있을 것이다.

• 한국콘텐츠학회논문지
• /
• 제20권1호
• /
• pp.562-572
• /
• 2020

본 연구는 동료학습을 적용한 조리실무관련 실습과목의 학습부진 대학생의 주관적 인식유형을 분석하여 조리실무 관련 수업의 보다 나은 교육효율성 제고를 위한 기초 자료를 제공하고자 한다. 또한 소규모 학생들의 주관적 인식에 대한 연구를 위해 Q방법론을 이용하여 분석하였으며, 주관적 인식들 사이에서 발견되는 공통적인 특징의 분석을 통해 총 3가지의 유형을 도출하였다. 제 1유형(N=8) : 학습효과 증대 형 (Increase learning effectiveness type), 제 2유형(N=8): 소극적 학생에 대한 수업자료개발 필요 (Development of lesson materials for passive students), 제3유형(N=6): 실습형 자기주도학습 개발 필요 (Practical self-directed learning needs development)은 각각 고유의 특징을 가진 유형으로 분석되었다. 결과적으로 조리실무관련 실습과목의 학습부진 학생들에게 동료학습을 적용함으로서 학습부진학생들의 수업흥미유발, 실수감소로 인한 자신감 상승 등의 학습효과증대와 추가학습에 대한 인식 증가와 주도적 학습 참여 유도와 같은 긍정적인 학습효과를 가져왔음을 발견하였으나, 일부학생의 경우 동료학생에 피해를 주는 것 같은 느낌과 타 학생들의 시선에 대한 부담감을 표현하는 집단이 발견되기도 했다. 연구 결과를 통해 수준별 학습 분위기가 조성된 학습 환경에서의 실습조리과정의 진행과 주도적 학습을 위한 추가 교육과정의 개발은 보다 효과적인 동료학습을 위한 추가적인 조건임을 발견하였다.