• 제목/요약/키워드: PAS-4

검색결과 317건 처리시간 0.026초

鷄胎賢의 Alkaline Phosphatase, PAS-陽性物質 및 燐脂質의 分布에 관하여 (Distribution of Alkaline Phosphatase, PAS-Positive Materials and Phospholipid in the Kidney of the Developing Chick)

  • 하재청
    • 한국동물학회지
    • /
    • 제12권3호
    • /
    • pp.77-84
    • /
    • 1969
  • 著者는 白色레그혼의 種卵을 孵化시켜 얻은 鷄胎中 賢과 後賢에 있어서의 alk. p., PAS-陽性物質 및 燐脂質의 消長과 局在位置의 變異를 組織化學的 方法으로 追究하여 다음과 같은 結果를 얻었다. 1. 分泌尿細管의 器官形成에 앞서 未分化 間葉組織內에 强한 alk. p. 活性이 出現하였으며 同活性은 分泌尿細管의 分化와 함께 消失하였다. 2. 中賢 및 後賢 分泌尿細管의 分化와 함께 alk. p. 및 PAS-陽性物質은 分泌尿細管의 刷子綠에 强陽性反應을 보였다. 3. 中賢 및 後賢 分泌尿細管의 細胞質과 刷子綠은 强한 燐脂質 陽性反應을 띠었다. 4. 造賢組織의 分化中 未分化 間葉組織에 alk. p. 活性이 出現하였다가 組織의 分化와 함께 消失된 事實에서 보아 alk. p. 는 組織分化에 關與하는 것으로 생각된다. 5. 中賢 및 後賢 分泌尿細管의 刷子  에 있어서 alk. p. 와 PAS-陽性物質 및 燐脂質의 變異와 分布狀態가 一致한 것으로 보아 alk. p. 는 炭水化物의 再吸收 및 燐脂質代謝에 關與하는 것으로 思料된다.

  • PDF

반추동물 적혈구막 단백의 전기영동법에 의한 분석 -낮은 적혈구침강속도와의 관계- (Electrophoretic analysis of the major proteins of ruminant erythrocyte membrane: Their relation to slow erythrocyte sedimentation rate)

  • 이방환;박영우
    • 대한수의학회지
    • /
    • 제29권4호
    • /
    • pp.445-455
    • /
    • 1989
  • The proteins of the ruminant erythrocyte membranes were analysed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate, and their relations to the slow erythrocyte sedimentation rate(ESR) of the ruminants were investigated by treating the erythrocytes with proteinases such as trypsin, chymotrypsin and pronase, and glycosidases such as neuraminidase and galactosidase. Protein content in the erythrocyte membrane was $2.85{\pm}0.28$ in human, $3.60{\pm}0.41$ in Korean cattle, $3.71{\pm}0.36$ in Holstein, $4.13{\pm}0.83$ in Korean native goat and $3.94{\pm}0.56mg/ml$ in sheep, showing higher in ruminant animals than in human(p<0.01). Although the general protein profiles of the ruminant erythrocyte membranes were almost similar to that of human, all the ruminant erythrocyte membranes showed one additional protein band, called band-Q in the previous report on proteins of bovine erythrocyte membrane, which migrated electrophoretically to the mid position between band-2 and band-3 in human erythrocyte membranes. The glycoprotein profiles of ruminant erythrocyte membranes revealed by periodic acid Schiff(PAS) stain showed a marked difference from that of human. The PAS-1(glycophorin) and PAS-2(sialoglycogrotein) present in human erythrocyte membranes were almost absent from the ruminant animals. Instead, a strong PAS-positive band near the origin of the electrophorograms, which was named as PAS-B in the previous report on proteins of bovine erythrocyte membranes, was shown in the ruminant animals except sheep. In addition, the erythrocyte membranes of Korean native goat and sheep showed a moderate PAS-negative band near the tracking dye of the electrophorograms, which was named as PAS-G in this study. In the erythrocyte treated with the enzymes, the migration of each protein fracture of erythrocyte membranes in response to each enzyme was diverse according to different species or breed of ruminant animals. Among others, band-Q present in ruminants was slightly or moderately decreased by trypsin-, chymotrypsin-, and pronase- treatments of the erythrocytes, but not only in sheep. It was particularly noticeable that PAS-B, a fraction of glycoprotein, present in ruminants except sheep, was better digested by proteinases than by glycosidases, showing remarkable increase(p<0.01) of the ESR in accord with complete digestion(disappearance) of the PAS-B band by pronase, trypsin or chymotrypsin treatment of erythrocytes. In sheep, there was almost no any response to the various enzymes in general protein and glycoprotein profiles of the erythrocyte membranes except PAS-G, which was markedly decreased by pronase treatment of the erythrocytes. Nevertheless, the ESRs were accelerated in erythrocytes treated with pronase, trypsin, chymotrypsin and neuraminidase. Erythrocyte osmotic fragility was increased in erythrocytes treated with only pronase among five enzymes in all the human and ruminant animals used in this study.

  • PDF

Design of Dualband Class-F PAs for Cellular and WLAN Applications

  • Lee, Chang-Min;Park, Young-Cheol;Yoon, Hoi-Jin
    • Journal of electromagnetic engineering and science
    • /
    • 제10권1호
    • /
    • pp.6-12
    • /
    • 2010
  • In this paper, highly efficient class-F power amplifiers(PAs) with harmonic-controlling transmission lines(TLs) were built for cellular and WLAN applications at 840 MHz and 2.4 GHz each. Also, based on these single-band PAs, a dualband class-F PA was designed after a careful investigation into the harmonics of the two frequencies. The harmonic-controlling TL was designed for the class-F operation at dualband by switching the length of the shunt $\lambda$/4 TL part, while the series $\lambda$/4 TL is optimized for both frequencies. To verify the performance, two class-F PAs optimized at each frequency and a dualband class-F PA at the corresponding frequencies were built with the secondand the third-harmonic control circuits at each frequencies. As a result, the PA#1 at 840 MHz has a peak drain efficiency of 81.2 % with an output power of 24.4 dBm, while the PA#2 at 2.35 GHz shows a drain efficiency of 94.5 % with an output of 22.8 dBm. Finally, the dualband class-F PA#3 showed 60.5 % and 50.9 % drain efficiencies at 840 MHz and 2.4 GHz, with powers of 23.8 dBm and 19.62 dBm, respectively.

동면에 따른 옴개구리(Rana rugosa) 위장관 점액분비세포에 관한 조직화학적 연구 (Histochemical Study on the Mucous Secreting Cells in the Gastrointestinal Mucosae of Rana rugosa according to Hibernation)

  • 정영화;노용태;김한화
    • 한국동물학회지
    • /
    • 제17권2호
    • /
    • pp.69-80
    • /
    • 1974
  • 동면 전, 중, 후 및 활동기간 동안 옴개구리(Rana rugosa)의 위장관 점액분비세포내 점액성 물질의 변화를 조직화학적으로 관찰하여 다음과 같은 결론을 얻었다. 1. 옴개구리의 위장관 점막내 점액성 물질은 위 표면상피세포에서 PAS에, 소장 배상 세포에서는 PAS와 AB 2.5에, 그리고 대장 배상세포에서는 AB 2.5에 강한 양성 반응을 보였다. 2. 동면에 따라 위 점막분비세포내 PAS 양성인 중성 점액질은 현저히 증가하였다. 3. 동면에 따라 소장내 AB 2.5에 양성인 약산성 점액질과 AB 1.0에 양성인 산성 유화 점액질은 현저히 감소를 보이는 반면, PAS 양성인 중성 점액질은 약간 증가를 보였다. 4. 동면에 따라 대장내 AB 2.5에 양성인 약산성 점액질은 아주 현저한 감소를 보였고, PAS양성인 중성 점액질도 역시 다소 감소를 보였다. 5. 동면시 위점막 상피세포내에서 PAS양성인 점액질은 그 분비능이 합성능에 미치지 못하여 현저히 증가되며, 위 점막의 접착을 방지하고, 위산 및 효소의 활성을 억제하는 것으로 생각되며, 장내 배상세포에서는 위산 및 장액분비의 현저한 감소로 장내 산도가 중성에 가까워져서 중성에 가까운 점액물질을 분비하는 것으로 추리된다.

  • PDF

Demographics, Social Media Use and Perceived Academic Stress of Secondary School Students in St. Thomas Aquinas College, Akure, Nigeria

  • Igbinovia, Magnus Osahon;Idhalama, Ogagaoghene Uzezi;Alex-Nmecha, Juliet C.
    • International Journal of Knowledge Content Development & Technology
    • /
    • 제9권4호
    • /
    • pp.7-29
    • /
    • 2019
  • The study was carried out to investigate the influence of demographics and social media use on perceived academic stress of secondary school students in St. Thomas Aquinas College, Akure, Nigeria. This was achieved using five research questions and four null hypotheses. The population of the study consisted of 1,107 students at ISCED level 3, out of which 286 were selected based on the Israel (2003) model for determining sample size. The Ex-Post Facto (EPT) research design of the correlational type was employed to investigate the study while questionnaire was used for data elicitation. Out of the 286 copies distributed, 192 (67% response rate) were retrieved and analyzed using descriptive statistics (frequency, percentage, mean and standard deviation) and inferential statistics (correlation and multiple regression). The findings revealed that there is high frequency of social media use among secondary school students, majorly for meeting new friends and chatting. The perceived academic stress (PAS) of the students was found to be moderate. Of the three demographics considered, only class had significant influence on PAS. Use of social media did not have significantly influence on PAS of the students. When combined, demographics and social media use predicted PAS; and when considered relatively, of the independent variables, only class as an aspect of demographics predicted PAS. Therefore, the authors concluded that PAS of secondary school students is not directly influenced by demographics (except for class) and social media use. Based on the study's conclusion, recommendations were made.

Pre-Coronavirus Disease 2019 Pediatric Acute Appendicitis: Risk Factors Model and Diagnosis Modality in a Developing Low-Income Country

  • Salim, Jonathan;Agustina, Flora;Maker, Julian Johozua Roberth
    • Pediatric Gastroenterology, Hepatology & Nutrition
    • /
    • 제25권1호
    • /
    • pp.30-40
    • /
    • 2022
  • Purpose: Pediatric acute appendicitis has a stable incidence rate in Western countries with an annual change of -0.36%. However, a sharp increase was observed in the Asian region. The Indonesian Health Department reveals appendicitis as the fourth most infectious disease, with more than 64,000 patients annually. Hence, there is an urgent need to identify and evaluate the risk factors and diagnostic modalities for accurate diagnosis and early treatment. This study also clarifies the usage of pediatric appendicitis score (PAS) for children <5 years of age. Methods: The current study employed a cross-sectional design with purposive sampling through demographic and PAS questionnaires with ultrasound sonography (USG) results. The analysis was performed using the chi-square and Mann-Whitney tests and logistic regression. Results: This study included 21 qualified patients with an average age of 6.76±4.679 years, weighing 21.72±10.437 kg, and who had been hospitalized for 4.24±1.513 days in Siloam Teaching Hospital. Compared to the surgical gold standard, PAS and USG have moderate sensitivity and specificity. Bodyweight and stay duration were significant for appendicitis (p<0.05); however, all were confounders in the multivariate regression analysis. Incidentally, a risk prediction model was generated with an area under the curve of 72.73%, sensitivity of 100.0%, specificity of 54.5%, and a cut-off value of 151. Conclusion: PAS outperforms USG in the sensitivity of diagnosing appendicitis, whereas USG outperforms PAS in terms of specificity. This study demonstrates the use of PAS in children under 5 years old. Meanwhile, no risk factors were significant in multivariate pediatric acute appendicitis risk factors.

Saponins from the Callus Mass of Phytolacca americana

  • Chi, Hyung-Joon;Kim, Hyun-Soo
    • Archives of Pharmacal Research
    • /
    • 제8권1호
    • /
    • pp.15-20
    • /
    • 1985
  • Strong anti-inflammatory saponins Phytolacca americana (Phytolaccaceae) wereobtained from callus mass derived from the stems and also from that derived from the roots of cultivated Phytolacca americana (which were designated as PAS and PAR, respectively). The callus were grown on Linsmair and Skoog's agar medium supplemented with 1ppm OF 2, 4-dichlorophenoxyacetic acid. Phytol accoside B and D were obtained from PAS and phytolaccoside A and B from PAR. The thin layer chromatograms of the crude saponins from PAS and PAR were similar to those of original plants. PAS contained phytolaccoside B as a major component while phytolaccoside E was a major saponin in original plants.

  • PDF

PAS 전극에 관한 전기화학적 특성 측정 (Electrochemical Property Measurement on Flyacenic Semiconductor(PAS))

  • 김한주;박수길;손원근;이홍기;이주성
    • 한국전기전자재료학회:학술대회논문집
    • /
    • 한국전기전자재료학회 1999년도 춘계학술대회 논문집
    • /
    • pp.197-200
    • /
    • 1999
  • The polyacene materials prepared from phenol resine at relatively low temperature(550~75$0^{\circ}C$) show a highly Li-doped state up to $C_2$Li state without liberation of Li cluster. We prepared each polyacenic materials various temperature and investigated electrochemical property. We tried to change the mole ratio of [H]/[C] that was 0.24~0.4 range and finally found that the further discussion of improvements of battery materials. The X-ray structural analyses have shown that this material is essentiallly amorphous with loose structure in molecular size order. This structure ensures that the PAS battery has both reliability on repetitive doping-undoping processes and higher energy density than other batteries. The PAS electrode has been confirmed to show good stability and reversibility.

  • PDF

점액 및 면역 세포화학적 Panel 염색에 의한 장액성 삼출액내 반응성 중피세포와 암종세포의 감별 (Distinction Between Reactive Mesothelial and Carcinoma Cells in Serous Effusions by Mucin- and Immune-cytochemical Panel)

  • 김병헌
    • 대한세포병리학회지
    • /
    • 제9권1호
    • /
    • pp.1-14
    • /
    • 1998
  • The cytologic distinction of carcinoma cells from reactive mesothelial cells can be difficult, especially in specimens containing abundant reactive mesotheilal cells and inflammatory cells with scant carcinoma cells. This study evaluates the usefulness of mucin and immunocytochemistry for discrimination between reactive mesotheilal cells and carcinoma cells, and sensitivity and specificity of these stains for the detection of metastatic carcinoma in serous effusions. Immunocytochemical panel including mucin cytochemistry with the periodic acid-Schiff(PAS) reaction after or without diastase digestion was undertaken on 127 serous effusion specimens with histologically confirmed diagnoses. The specimens including cell smears and cell blocks were stained with PAS and antibodies to carcinoembryonic antigen(CEA), epithelial membrane antigen(EMA), cytokeratln(CK), and vimentin. The sensitivities of these stains for metastatic carcinoma(127 cases) were 49%(46/94) in PAS, 48%(60/124) in CEA, 89%(97/109) in EMA, 88%(93/106) in CK, and 25%(20/81) in vimentin. The sensitivities of stains for reactive mesothelial cells(36 cases) were 19%(7/36) in EMA, 78%(28/36) in CK, and 75%(27/36) in vimentin. The PAS and CEA stains were not reacted with all cases of benign reactive serous effusions containing abundant reactive mesothelial cells. The specificities of stains for metastatic carcinoma(127 cases) were 100% in PAS, 100% in CEA, 81% in EMA, 22% in CK, and 25% in vimentin. The optimal combination of stains for use in a panel was PAS and CEA. Combined results from these two stains yielded an advanced sensitivity of 8% in PAS and 4% in CEA for metastatic carcinoma. EMA was also cosiderably useful for identification of carcinoma cells. CK and vimentin were not suitable for distinguishing between reactive mesothelial cells and carcinoma cells.

  • PDF

장애인활동지원서비스제도의 노동시장 참여에 대한 정책효과 (The effect of policy on Korean personal assistance service for persons with disabilities of labor market participation)

  • 김송숙;김유민;나가연;백승희;이근철
    • 한국융합학회논문지
    • /
    • 제12권4호
    • /
    • pp.267-274
    • /
    • 2021
  • 본 연구는 장애인활동지원제도가 정책효과로서 수급자 가구원의 노동시장 참여를 촉진시켰는지에 대해 평가하고자 한국복지패널 6차(2011년)과 12차(2017년)자료를 이용하였다. 연구대상은 성향점수매칭 중 Caliper matching을 이용하여 제도를 이용한 실험집단 64명과 이용하지 않은 통제집단 344명을 선정하였다. 집단간 특성차이는 카이제곱검정을 이용하였고, 제도 전후의 정책효과를 추정하기 위해 단순이중차이분석과 이중차이 다중회귀분석을 실시하여 제도의 노동시장 참여에 대한 영향을 파악하였다. 연구결과 장애인활동지원제도는 이용자 가구원의 노동시장 참여에 대한 유의한 차이가 나타나지 않았다. 이는 제도 이용자의 수의 적음으로 인한 낮은 통계적 검정력과 불완전한 매칭 그리고 장애인 활동지원사의 서비스만으로는 장애인 자립에 한계가 있기 때문으로 생각한다. 따라서 제도의 효과를 나타내기 위해 전문화된 서비스와 장애인과 부양가구원의 요구에 맞는 제도가 실시되어야 할 것이다.