• 대한임상검사과학회지
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• 제48권2호
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• pp.74-81
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• 2016

Fluoroquinolone 계 항생제의 광범위한 사용으로 인해 이 약제에 대한 내성 Ureaplasma 종의 분리 비율이 높아지고 있다. Fluoroquinolone 계 항생제 내성은 주로 DNA gyrase와 topoisomerase IV 유전자의 돌연변이로 인해 발생하는 것으로 알려져 있다. DNA gyrase는 A와 B 2개의 소단위로 이루어져 있으며, gyrA와 gyrB 유전자에 의해 암호화되어 있고, Topoisomerase IV는 parC와 parE 유전자에 의해 암호화되어 있다. 본 연구가 진행된 서울의 1개 3차 병원에서 2012년부터 2013년까지 1년동안 Ureaplasma 종의 fluoroquinolone 계 항생제인 OFL과 CIP의 항생제검사 감수성 결과를 분석한 결과 내성과 중등도를 합산할 경우 66.08%, 92.69%로 매우 높은 내성 비율을 보였다. 이에 Ureaplasma 종을 OFL과 CIP에 대한 감수성을 기준으로 4개 그룹으로 분류하여 gyrA, gyrB, parC, parE 유전자의 돌연변이 여부를 검사하여 항생제 내성과의 관련성을 밝히고자 하였다. 그 중 parC 유전자의 돌연변이 빈도가 높아 topoisomerase IV의 돌연변이가 fluoroquinolone 계 약제에 대한 내성과 밀접한 관련이 있음을 확인할 수 있었다. 본 연구를 통해 GyrB의 Asn481Ser, ParC의 Phe149Leu, Asp150Met, Asp151Ile, Ser152Val, ParE의 Pro446Ser, Arg448Lys을 추가로 발견할 수 있었다. 최근 fluoroquinolone 계 항생제의 사용이 증가하고 있기 때문에 추후 Ureaplasma 종의 fluoroquinolone 계 항생제 내성에 대한 지속적인 모니터링이 필수적일 것으로 사료되며, 이와 관련한 유전자의 돌연변이 양상과의 상관관계를 분석하여 기존 배양검사의 단점을 보완할 수 있는 분자 진단학적 검사법의 추가적인 분석이 필요할 것으로 사료된다.

• 대한의생명과학회지
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• 제26권2호
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• pp.120-126
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• 2020

Fluoroquinolone (FQ) resistant uropathogenic Escherichia coli (UPEC) have become a major problem in urinary tract infections (UTIs). The purpose of this study was to compare the quinolone resistance-determining region (QRDR) and plasmid mediated quinolone resistance (PMQR) determinants of FQ resistant UPEC between 1989 and 2010-2014. A total of 681 strains of UPEC clinical isolates was collected from Korean healthcare facility in 1989 (123 strains) and in 2010-2014 (558 strains). The minimum inhibitory concentrations (MICs) of FQs were determined by agar dilution method. QRDRs (gyrA, gyrB, parC and parE) and PMQR determinants (qnrA, qnrB, qnrS, aac(6')-Ib-cr and qepA) were analyzed polymerase chain reaction and sequencing method. Among 681 isolates, FQ resistant UPEC were 3 strains (2.4%) in 1989 isolates and 220 strains (39.4%) in 2010-2014 isolates. The rate of the FQ resistant UPEC strains in 2010-2014 isolates was increased than that of in 1989 isolates. UPEC isolates from 1989 and 2010-2014 were shown to carry mutations in gyrA (Ser83 and Asp87), gyrB (Ser464 and Thr469), parC (Ser80 and Glu84) and parE (Glu460, Ser458, Ile464 and Leu445). The most common mutations of QRDRs in 1989 isolates were Ser83Leu and Asp87Gly in gyrA and Ser80Ile in parC (2 strains: 66.7%) while those in 2010-2014 isolates were Ser83Leu and Asp87Asn in gyrA and Ser80Il2 and Glu84Val in parC (88 strains: 40.0%). PMQR determinants were detected only in 2010-2014 UPEC strains (47 strains: 21.4%).

• 한국정보처리학회논문지
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• 제2권4호
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• pp.554-560
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• 1995

본 논문은 크기가 n와 k인 nheap과 kheap을 병합시키기 위한 병렬 알고리즘을 제 시함과 동시에 그들을 MasPar상에 실제로 구현하고자 하는데 그 주된 목적이 있다. 이때, EREW-PRAM(Exclusive-Read Exclusive-Write Parallel Random Acess Machin)상에 서 max(2$^{-1}$, $\ulcorner$(m＋1)/4$\lrcorner$개의 프로세서를 이용해서 본 논문에 제시된 알고리즘 의 시간 복잡도가 O(log(n/k)＊log(n))임을 제시하였다. 여기서 i는 heap의 height를 뜻하며, m은 크기 n과 k의 합으로 구성된 것이다. 또한 이것을 MasPar 컴퓨터에 적용 을 시켰을 때, 테이타의 양이 8백만개이고, 64개의 프로세서를 이용한 경우의 speedup 을 33.934를 얻었다. 이때 적용된 데이타의 형태는 불완전 힙상에서 크기가 k〈n를 지 니는 경우의 처리이다. 그리고 이같이 제시된 알고리즘의 EPU(Effective Processor Utilization)을 계산하면 1인 최적의 speedup율을 나타냄을 알 수가 있다.

• 한국작물학회지
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• 제35권2호
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• pp.156-164
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• 1990

A field experiment was conducted to study both the distribution characteristics of photosynthetically active radiation (PAR) in the soybean canopy and their relationships with dry matter production. The soybean cultivars 'Hwanggeumkong' and 'Paldalkong' were sown with the spaces of 60$\times$15cm and 30$\times$15cm at Suwon on May 20 and on June 20 in 1989. The ratio of PAR to the total shortwave radiation was estimated by the empirical equation derived from sunshine hours and direct incoming radiation. The functional relationships between the PAR interception and the leaf area index were expressed as a function of Beer's law. The extinction coefficients(k) in the functions ranged from 0.77 to 0.92. The values of k were greater at higher planting density, but they were affected neither by planting dates nor by varieties. The reflection ratio of PAR($\alpha$) was determined by the exponential function as below; $\alpha$=$\alpha$p-($\alpha$p-$\alpha$o) exp(-kㆍLAI) where $\alpha$p was the reflectance at the maximum LAI and $\alpha$o was that of the bare soil. The ap ranged from 0.025 to 0.035 and $\alpha$o ranged from 0.11 to 0.12, respectively. The reflected PAR ranged from 0.049 to 0.064 and the transmitted PAR ranged from 0.168 to 0.340 until maximum dry weights were observed. The slope from the linear regression of dry matter on absorbed PAR, conversion efficiency, ranged from 1.30 to 2.3g MJ$^{-1}$ during the growing season until maximum dry weight was reached. The total dry matter yield above ground (TDM) increased with the increases in the conversion efficiency. TDM was higher in Hwanggeumkong than Paldalkong and higher in the space of 30$\times$15cm than 60$\times$15cm, Paldalkong showed higher harvest index than Hwanggeumkong. than Hwanggeumkong.

• Parasites, Hosts and Diseases
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• 제49권3호
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• pp.235-243
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• 2011

In order to get a better understanding of the role of protease-activated receptor 2 (PAR2) in type 2 helper T (Th2) cell responses against Trichinella spiralis infection, we analyzed Th2 responses in T. spiralis-infected PAR2 knockout (KO) mice. The levels of the Th2 cell-secreted cytokines, IL-4, IL-5, and IL-13 were markedly reduced in the PAR2 KO mice as compared to the wild type mice following infection with T. spiralis. The serum levels of parasite-specific IgE increased significantly in the wild type mice as the result of T. spiralis infection, but this level was not significantly increased in PAR2 KO mice. The expression level of thymic stromal lymphopoietin, IL-25, and eotaxin gene (the genes were recently known as Th2 response initiators) of mouse intestinal epithelial cells were increased as the result of treatment with T. spiralis excretory-secretory proteins. However, the expression of these chemokine genes was inhibited by protease inhibitor treatments. In conclusion, PAR2 might involve in Th2 responses against T. spiralis infection.

• 가정과삶의질연구
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• 제9권2호
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• pp.241-251
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• 1991

The Purpose of this study was the development of scale to measure the Perceived Adequacy of Resources(PAR). A 38-item instrument was analysed with responses form 300 wives dwelling in Seoul. PAR was categorized housing, health, time money, interpersonal, knowledge/skills, community resources, Data were analysed by the method of Pearson's Correlation, Factor Analysis and Multiple Regression. Items were loaded eight resource categories such as time housing, money , health, knowledge, social support, community, psychological relationship. Internal consistency of the scale was high(Cronbach's α=.85) The result proposed PAR constructed 28 items.

• 한국약용작물학회지
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• 제13권6호
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• pp.245-249
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• 2005

신이는 전통적으로 비염 등의 치료에 사용되어 왔는데, 신이 에탄올 추출물은 그람 양성균인 Staphylococcus aureus 그람 음성균인 Pseudomonas fluorescens균 및 곰팡이균인 Aspergillus niger에 대하여 모두 항균활성을 보였으며, proteinase로 활성화된 receptor-2에 의한 흰쥐 뒷발바닥 부종모델에서 신이 에탄올 추출물을 경구투여한 경우 typsin이나 $tc-NH_2$로 유발된 발바닥 부종, 혈관투과성, myoloperoxidase 활성도에서 유의성 있는 억제율을 보여 항염증 활성이 뚜렷함을 나타냈다.

• Journal of Microbiology
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• 제43권5호
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• pp.391-397
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• 2005

Escherichia coli is a common inhabitant of the intestinal tracts of animals and humans. The intestines of animals also represent an ideal environment for the selection and transfer of antimicrobial resistance genes. The aim of this study was to investigate the resistance of E. coli isolated from chicken fecal samples to fluoroquinolones and to analyze the characterization of mutations in its gyrA and parC gene related resistance. One hundred and twenty-eight E. coil isolates showed a high resistance to ciprofloxacin (CIP; $60.2\%$), enrofloxacin (ENO; $73.4\%$) and norfloxacin (NOR; $60.2\%$). Missense mutation in gyrA was only found in the amino acid codons of Ser-83 or Asp-87. A high percentage of isolates ($60.2\%$) showed mutations at both amino acid codons. Missense mutation in parC was found in the amino acid codon of Ser-80 or Glu-84, and seven isolates showed mutations at both amino acid codons. Isolates with a single mutation in gyrA showed minimal inhibitory concentrations (MIC) for CIP (${\le}0.5\;to\;0.75{\mu}g/ml$), ENO (1 to $4{\mu}g/ml$) and NOR (0.75 to $4{\mu}g/ml$). These MIC were level compared to isolates with two mutations, one in gyrA and one in parC, and three mutations, one in gyrA and two in parC (CIP, ${\le}0.5\;to\;3{\mu}g/ml;\;ENO,\;2\;to\;32<{\mu}g/ml;\;NOR,\;1.5\;to\;6\;{\mu}g/ml$). However, the isolates with two mutation in gyrA regardless of whether there was a mutation in parC showed high MIC for the three fluoroquinolones (CIP, 0.75 to $32{\le}{\mu}g/ml;\;ENO,\;3\;to\;32{\le}{\mu}g/ml;\;NOR,\;3\;to\;32{\le}{\mu}g/ml$). Interestingly, although the E. coil used in this study was isolated from normal flora of chicken, not clinical specimens, a high percentage of isolates showed resistance to fluoroquinolones and possessed mutations at gyrA and parC associated with fluoroquinolone resistance.

• 생물환경조절학회지
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• 제30권4호
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• pp.401-409
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• 2021

본 연구는 반밀폐형 토마토 재배 온실에서 광합성율 극대화를 위한 적정 탄산가스 시비 농도를 구명하고자 광합성 모델을 이용하여 잎의 최대 카복실화율(V_cmax), 최대 전자전달속도(J_max), 열파괴, 잎 호흡 등을 계산하고 실제 측정값과 비교하였다. 다양한 광도(PAR 200µmol·m^-2·s^-1 to 1500µmol·m^-2·s^-1)와 온도(20℃ to 35℃) 조건에서 CO₂ 농도에 대한 A-C_i curve는 광합성 측정 기기를 사용하여 측정하였고, 모델링 방정식으로 아레니우스 함수값(Arrhenius function), 순광합성율(net CO₂ assimilation, A_n), 열파괴(thermal breakdown), R_d(주간의 잎호흡)를 계산하였다. 엽온이 30℃ 이상으로 상승하였을 때 J_max, An 및 thermal breakdown 예측치가 모두 감소하였고, 예측 J_max의 가장 최고점은 엽온 30℃였으며 그 이상의 온도에서는 감소하였다. 생장점 아래 5번째 잎의 광합성율은 PAR 200-400µmol·m^-2·s^-1 수준에서는 CO₂ 600ppm, PAR 600-800µmol·m^-2·s^-1 수준에서는 CO₂ 800ppm, PAR 1000µmol·m^-2·s^-1 수준에서는 CO₂ 1000ppm, PAR 1200-1500µmol·m^-2·s^-1 수준에서는 CO₂ 1500ppm을 공급했을 때 포화점에 도달하였다. 앞으로 광합성 모델식을 활용하여 과채류 온실 재배 시 광합성을 높일 수 있는 탄산시비 농도를 추정할 수 있을 것으로 판단된다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3793-3798
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• 2013

The role of protease-activated receptors (PARs) in lung tumors is controversial. Although PAR4 is preferentially expressed in human lung tissues, its possible significance in lung cancer has not been defined. The studies reported herein used a combination of clinical observations and molecular methods. Surgically resected lung adenocarcinomas and associated adjacent normal lung tissues were collected and BEAS-2B and NCI-H157 cell lines were grown in tissue culture. PAR4 expression was evaluated by RT-PCR, RT-qPCR, Western blotting and immunohistochemistry analysis. The results showed that PAR4 mRNA expression was generally decreased in lung adenocarcinoma tissues as compared with matched noncancerous tissues (67.7%) and was associated with poor differentiation (p=0.017) and metastasis (p=0.04). Western blotting and immunohistochemical analysis also showed that PAR4 protein levels were mostly decreased in lung adenocarcinoma tissues (61.3%), and were also associated with poor differentiation (p=0.035) and clinical stage (p=0.027). Moreover, PAR4 expression was decreased in NCI-H157 cells as compared with BEAS-2B cells. In conclusion, PAR4 expression is significantly decreased in lung adenocarcinoma, and down-regulation of PAR4 is associated with a more clinically aggressive phenotype. PAR4 may acts as a tumor suppressor in lung adenocarcinoma.