Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Comparison of Fluoroquinolone Resistance Determinants in Uropathogenic Escherichia coli between 2 Time Periods of 1989 and 2010-2014 at Gangwon Province in Korea

  • Park, Min (Department of Biomedical Laboratory Science, Daekyeung University)
  • Received : 2020.06.16
  • Accepted : 2020.06.25
  • Published : 2020.06.30
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Abstract

Fluoroquinolone (FQ) resistant uropathogenic Escherichia coli (UPEC) have become a major problem in urinary tract infections (UTIs). The purpose of this study was to compare the quinolone resistance-determining region (QRDR) and plasmid mediated quinolone resistance (PMQR) determinants of FQ resistant UPEC between 1989 and 2010-2014. A total of 681 strains of UPEC clinical isolates was collected from Korean healthcare facility in 1989 (123 strains) and in 2010-2014 (558 strains). The minimum inhibitory concentrations (MICs) of FQs were determined by agar dilution method. QRDRs (gyrA, gyrB, parC and parE) and PMQR determinants (qnrA, qnrB, qnrS, aac(6')-Ib-cr and qepA) were analyzed polymerase chain reaction and sequencing method. Among 681 isolates, FQ resistant UPEC were 3 strains (2.4%) in 1989 isolates and 220 strains (39.4%) in 2010-2014 isolates. The rate of the FQ resistant UPEC strains in 2010-2014 isolates was increased than that of in 1989 isolates. UPEC isolates from 1989 and 2010-2014 were shown to carry mutations in gyrA (Ser83 and Asp87), gyrB (Ser464 and Thr469), parC (Ser80 and Glu84) and parE (Glu460, Ser458, Ile464 and Leu445). The most common mutations of QRDRs in 1989 isolates were Ser83Leu and Asp87Gly in gyrA and Ser80Ile in parC (2 strains: 66.7%) while those in 2010-2014 isolates were Ser83Leu and Asp87Asn in gyrA and Ser80Il2 and Glu84Val in parC (88 strains: 40.0%). PMQR determinants were detected only in 2010-2014 UPEC strains (47 strains: 21.4%).

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References

  1. Betitra Y, Teresa V, Miguel V, Abdelaziz T. Determinants of Quinolone Resistance in Escherichia coli Causing Community-Acquired Urinary Tract Infection in Bejaia, Algeria. Asian Pac J Trop Med. 2014. 7: 462-467. https://doi.org/10.1016/S1995-7645(14)60075-4
  2. Cattoir V, Poirel L, Rotimi V, Soussy CJ, Nordmann P. Multiplex PCR for detection of plasmid-mediated quinolonone resistance qnr genes in ESBL-producing enterobacterial isolates. J Antimicrob Chemother. 2007. 60: 394-397. https://doi.org/10.1093/jac/dkm204
  3. Chang IH, Bang SH, Choi NY, Park SY, Han JH, Ahn SH. Trends in the Emergence of Ciprofloxacin-resistant Escherichia coli and the Relationship with Underlying Diseases in Patients with Urinary Tract Infection. Korean J Urol. 2008. 49: 66-71. https://doi.org/10.4111/kju.2008.49.1.66
  4. Dehbanipour D, Khanahmad H, Sedighi M, Bialvaei AZ, Faghri J. High Prevalence of Fluoroquinolone-Resistant Escherichia coli Strains Isolated From Urine Clinical Samples. J Prev Med Hyg. 2019. 29; 60: E25-E30.
  5. Fihman V, Lartigue MF, Jacquier H, Meunier F, Schnepf N, Raskine L, Riahi J, Sanson-le Pors MJ, Bercot B. Appearance of aac(6')-Ib-cr gene among extended-spectrum beta-lactamaseproducing Enterobacteriaceae in a French hospital. J Infect. 2008. 56: 454-459. https://doi.org/10.1016/j.jinf.2008.03.010
  6. Kang CI, Kim JE, Park DW, Kim BN, Ha US, Lee SJ, Yeo JK, Min SK, Lee HY, Wie SH. Clinical Practice Guidelines for the Antibiotic Treatment of Community-Acquired Urinary Tract Infections. Infect Chemother. 2018. 50: 67-100. https://doi.org/10.3947/ic.2018.50.1.67
  7. Li D, Liu B, Guo X, Liu F, Feng L, Wang L. A multiplex PCR method to detect 14 Escherichia coli serogroups associated with urinary tract infections. J Microbiol Methods. 2010. 82: 71-77. https://doi.org/10.1016/j.mimet.2010.04.008
  8. Liu X, Boothe DM, Thungrat K, Aly S. Mechanisms accounting for fluoroquinolone multidrug resistance Escherichia coli isolated from companion animals. Vet Microbiol. 2012. 28; 161: 159-168. https://doi.org/10.1016/j.vetmic.2012.07.019
  9. Liu CS, Yoon EJ, Kim DK, Shin JH, Shin JH, Shin KS, Kim YA, Uh Y, Kim HS, Kim YR, Jeong SH. Antimicrobial resistance in South Korea: A report from the Korean global antimicrobial resistance surveillance system (Kor-GLASS) for 2017. J Infect Chemother. 2019. 25: 845-859. https://doi.org/10.1016/j.jiac.2019.06.010
  10. Le TM, Baker S, Le TP, Le TP, Cao TT, Tran TT, Nguyen VM, Campbell JI, Lam MY, Nguyen TH, Nguyen VV, Farrar J, Schultsz C. High prevalence of plasmid-mediated quinolone resistance determinants in commensal members of the Enterobacteriaceae in Ho Chi Minh City, Vietnam. J Med Microbiol. 2009. 58: 1585-1592. https://doi.org/10.1099/jmm.0.010033-0
  11. Lee SJ, Cho YH, Kim BW, Lee JG, Jung SI, Lee SD, Lee SE, Kim ME, Choi YD, Rim JS, Sim BS, Cho IR, Ryu SB, Kim CS, Kim WJ, Lee TY. A Multicenter Study of Antimicrobial Susceptibility of Uropathogens Causing Acute Uncomplicated Cystitis in Woman. Korean J Urol. 2003. 44: 697-701.
  12. Lee JH, Subhadra B, Son YJ, Kim DH, Park HS, Kim JM, Koo SH, Oh MH, Kim HJ, Choi CH. Phylogenetic group distributions, virulence factors and antimicrobial resistance properties of uropathogenic Escherichia coli strains isolated from patients with urinary tract infections in South Korea. Letters in Applied Microbiology. 2015. 62: 84-90. https://doi.org/10.1111/lam.12517
  13. Machuca J, Ortiz M, Recacha E, Diaz-De-Alba P, Docobo-Rerez F, Rodriguez-Martinez JM., Pascual A. Impact of AAC(6')-Ib-cr in combination with chromosomal-mediated mechanisms on clinical quinolone resistance in Escherichia coli. J Antimicrob Chemother. 2016. 71: 2066-3071. https://doi.org/10.1093/jac/dkw274
  14. Nakano R, Nakano A, Abe M, Nagano N, Ashara M, Furukawa T, Ono Y, Yano H, Okamoto R. Prevalence and mechanism of fluoroquinolone resistance in clinical isolates of Proteus mirabilis in Japan. Heliyon. 2019. 2; 5: e10291.
  15. Patel J, Cockerill III F, Eliopoulos G, Jenkins S, Lewis II J, Limbago B, Nicolau D, Patel R, Powell M, Richter S, Swenson J, Traczewaki M, Turnidge J, Weinstein M, Zimmer B. Performance standards for antimicrobial susceptibility testing. 2016. 26th ed. pp. 52-60. Clinical Laboratory Standards Institute. Wayne, PA, USA.
  16. Rehman A, Patrick WM., Lamont IL. Mechanisms of Ciprofloxacin Resistance in Pseudomonas aeruginosa: New Approaches to an Old Problem. J Med Microbiol. 2019. 68: 1-10. https://doi.org/10.1099/jmm.0.000873
  17. Song SW, Lee EY, Koh EM, Ha HS, Jeong HJ, Bae IK, Jeong SH. Antibiotic resistance mechanisms of Escherichia coli isolates from urinary specimens. Korean J Lab Med. 2009. 29: 17-24. https://doi.org/10.3343/kjlm.2009.29.1.17
  18. Sung JY. Analysis of Quinolone Resistance Determinants in Escherichia coli Isolated from Clinical Specimens and Livestock Feces. Korean J Clin Lab Sci. 2018. 50: 422-430. https://doi.org/10.15324/kjcls.2018.50.4.422
  19. Takahashi A, Muratani T, Yasuda M, Takahashi S, Monden K, Ishikawa K, Kiyota H, Arakawa S, Matsumoto T, Shima H, Kurazono H, Yamamoto S. Genetic profiles of fluoroquinoloneresistant Escherichia coli isolates obtained from patients with cystitis: phylogeny, virulence factors, PAIusp subtypes, and mutation patterns. J Clin Microbiol. 2009. 47: 791-795. https://doi.org/10.1128/JCM.01740-08
  20. Vila J, Ruiz J, Goni P, De Anta MT. Detection of Mutations in parC in Quinolone-Resistant Clinical Isolates of Escherichia coli. Antimicrob Agents Chemother. 1996. 40: 491-493. https://doi.org/10.1128/AAC.40.2.491
  21. Yang HS, Kim YJ, Cho SY, Nam YS, Oh TS, Park KS, Kim HC, Lee HJ. Fluoroquinolone Resistance Mechanisms by Molecular Epidemiologic Study of Ciprofloxacin-Nonsusceptible Escherichia coli Sequence Types Isolated from Clinical Specimens in a Tertiary Care University Hospital in Korea: Emergence of Clone ST131, 2006-2008. Ann Clin Lab Sci. 2017. 47: 511-515.