• 한국산학기술학회논문지
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• 제6권3호
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• pp.255-260
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• 2005

PAHs는 2개 이상의 벤젠링을 가지는 방향족 탄화수소로, 특성과 분자량에 의해 크게 두 가지로 구분되며, 독성 유기물질들이 직$\cdot$간접적으로 인간에게 피해를 준다. 이러한 PAHs에 오염된 토양을 복구하기 위해서 PAHs를 완전분해 또는 무독성 화합물로 전환시켜야 한다. 본 연구에서는 PAHs분해를 위해 Bacillus subtilis을 사용하였고, 담체로 코르크분말과 톱밥을 선정하였다. Phenanthrene을 분해한 경우 코르크분말 담체와 톱밥담체가 비슷한 분해양상을 보였으며 , pyrene을 분해한 두 담체의 경우 톱밥 담체가 우수하다고 판명되었다. 또한 토양함수율은 분해제거효율의 주 영향요소로서 $45\~55\%$의 함수율 일 때 최적의 효율을 나타냈다.

• 미생물학회지
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• 제37권2호
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• pp.120-123
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• 2001

Sphingomonas chungbukensis DJ77에서 난분해성 물질 분해 유전자가 chromosome 또는 plasmid 존재하는지를 규명하였다. 야생주 DJ77의 plasmid를 mitomycin C를 이용하여 curing 시킨 후, 각각 phenanthrene과 biphenyl이 단일 탄소원으로 첨가된 최소배지에서 배양한 결과 야생주는 성장을 하지만 plasmid가 제거된 DJ77은 성장하지 않았다. 각각의 plasmid DNA를 분리한 수 이미 클로닝된 방향족 탄화수소 분해에 관련된 DNA를 probe로 하여 Southern hybridization을 한 결과 야생주에서만 positive signal을 발견할 수 있었다.

• 펄프종이기술
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• 제35권1호
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• pp.27-32
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• 2003

To understand the chemical structure of sewer sludge in comparison with commercial compost and charcoal used as a soil improver, it was carried out to analyse their ash contents and metal ions, and to elucidate the chemical structure of their residuals after a sequential treatment of alcohol-benzene(1:2) extraction in Soxhlet, 3% HCl reflux and 79% H₂SO₄ hydrolysis, using CHNS analyzer and solid C-13 NMR spectrometer. The results obtained were as follows: 1. Ash content of sludge was about 46% that is higher than those of compost (17%) and charcoal (4%). 2. The residual of sludge after a sequential treatment of HCl and H₂SO₄ hydrolyses had high ash content about 23%, too. 3. The sludge seems to be suitable to the soil improver because the content of heavy metal ions in sludge was near the compost and below the organic fertilizer standard. 4. Elemental composition of sludge residual after HCl-H₂SO₄ hydrolyes was C/sub 56/H/sub 91/O/sub 12/N₂S = (C/sub 6/H/sub 10/O/sub 5/)/sub 7/(C/sub 6/H₄)/sub 7/C₂H/sub 43/O₂N₂S, similar to C/sub 103/H/sub 122/O/sub 33/N/sub 6/S = (C/sub 6/H/sub 10/O/sub 5/)/sub 6/(C/sub 6/H₄)/sub 10/C/sub 7/H/sub 22/O₃N/sub 6/S of compost. 5. The sludge residual had proved to have both considerable aliphatic and aromatic groups, but the compost residual to have mainly aliphatic groups and the charcoal to have mainly aromatic groups, through the peak analysis of solid C-13 NMR charts. 6. So, the sewer sludge is proved to have a considerable amount of aromaticity like in woody biomass containing lignin.

• Toxicological Research
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• 제22권1호
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• pp.9-13
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• 2006

Diesel exhausted particles (DEP), a kind of fine particles with aerodynamic diameters less than $2.5{\mu}m$ (PM2.5), is of great concern to human health because they remain in atmosphere for long periods, invade an indoor air environment, and can be breathed most deeply into lung and reached the alveoli because of their small size ($0.1{\sim}0.4\;{\mu}m$ in diameter). Epidemiological and experimental studies suggested that DEP may play an active role in the increased respiratory mortality and morbidity. In addition to their physical characteristics, the chemical components including polyaromatic hydrocarbon (PAH) are regarded as a carcinogen causing pulmonary tumors. PLD plays an important role in cell proliferation with various physiological phenomena and affects other enzymes by activating signal transduction pathway. We investigated the cytotoxic mechanism of DEP on RAW 264.7 cells focusing on the role in activation of PLD. Our results suggested DEP induced PLD activity through a specific signaling pathway involving phospholipase $A_2$, PLC, PKC and $Ca^{2+}$ mobilization.