• Title/Summary/Keyword: P700

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Potential immune-modulatory effects of wheat phytase on the performance of a mouse macrophage cell line, Raw 264.7, exposed to long-chain inorganic polyphosphate

  • An, Jeongmin;Cho, Jaiesoon
    • Animal Bioscience
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    • v.34 no.3_spc
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    • pp.463-470
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    • 2021
  • Objective: This experiment was conducted to find out the immunological effects of wheat phytase when long-chain inorganic polyphosphate (polyP) treated with wheat phytase was added to a macrophage cell line, Raw 264.7, when compared to intact long-chain polyP. Methods: Nitric oxide (NO) production of Raw 264.7 cells exposed to P700, a long-chain polyP with an average of 1,150 phosphate residues, treated with or without wheat phytase, was measured by Griess method. Phagocytosis assay of P700 treated with or without phytase in Raw 264.7 cells was investigated using neutral red uptake. The secretion of tumor necrosis factor α (TNF-α) by Raw 264.7 cells with wheat phytase-treated P700 compared to intact P700 was observed by using Mouse TNF-α enzyme-linked immunosorbent assay kit. Results: P700 treated with wheat phytase effectively increased NO production of Raw 264.7 cells by 172% when compared with intact P700 at 12 h exposure. At 5 mM of P700 concentration, wheat phytase promoted NO production of macrophages most strongly. P700, treated with wheat phytase, stimulated phagocytosis in macrophages at 12 h exposure by about 1.7-fold compared to intact P700. In addition, P700 treated with wheat phytase effectively increased in vitro phagocytic activity of Raw 264.7 cells at a concentration above 5 mM when compared to intact P700. P700 dephosphorylated by wheat phytase increased the release of TNF-α from Raw 264.7 cells by 143% over that from intact P700 after 6 h exposure. At the concentration of 50 μM P700, wheat phytase increased the secretion of cytokine, TNF-α, by 124% over that from intact P700. Conclusion: In animal husbandry, wheat phytase can mitigate the long-chain polyP causing damage by improving the immune capabilities of macrophages in the host. Thus, wheat phytase has potential as an immunological modulator and future feed additive for regulating immune responses caused by inflammation induced by long-chain polyP from bacterial infection.

Selective Removal of Al(III) from Rare Earth Solutions Using Peas-based Activated Carbon

  • An, Fu-Qiang;Wu, Rui-Yan;Li, Min;Yuan, Zhi-Guo;Hu, Tuo-Ping;Gao, Jian-Feng
    • Journal of the Korean Chemical Society
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    • v.61 no.5
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    • pp.231-237
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    • 2017
  • Efficiently removing Al(III) from rare earth is very significant because even trace amount of Al(III) can cause serious harm to the rare earth materials. In this paper, a nitrogen-containing activated carbon, AC-P700, was synthesized using peas as raw materials. The AC-P700 was characterized by surface area analyzer, FT-IR, and XPS methods. The adsorption and recognition properties of AC-P700 towards Al(III) were investigated, and the recognition mechanism was also analyzed. The BET special surface area of AC-P700 was $1277.1m^2{\cdot}g^{-1}$, and the average pore diameter was 1.90 nm. The AC-P700 possesses strong adsorption affinity and excellent recognition selectivity towards Al(III). The adsorption capacity for Al(III) could reach to $0.53mmol{\cdot}g^{-1}$, and relative selectivity coefficients relative to La(III) and Ce(III) is 9.6 and 8.7, respectively. Besides, AC-P700 possesses better regeneration ability and reusability.

Removal of Fluoride Using Thermally Treated Activated Alumina (고온 처리된 활성알루미나를 이용한 불소 제거)

  • Park, Seong-Jik;Kim, Jae-Hyeon;Lee, Chang-Gu;Park, Jeong-Ann;Choi, Nag-Choul;Kim, Song-Bae
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.10
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    • pp.986-993
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    • 2010
  • In this study, sorption characteristics of thermally treated activated alumina (AA) for fluoride were investigated. Sorption experiments have been conducted in equilibrium and kinetic batch conditions. Also, effects of solution pH and anions on fluoride removal have been observed. The properties of thermally treated ( $700^{\circ}C$) activated alumina (AA700) and untreated activated alumina (UAA) were compared using field-emission scanning electron microscope, energy-dispersive spectrometry, X-ray diffractometer (XRD) analysis, and Brunauer-Emmett-Teller (BET) analysis. From the experiments using AA thermally treated at different temperatures (100, 300, 500, $700^{\circ}C$), it was found that at high fluoride concentrations (50, 100, 200 mg/L) the sorption capacity of thermally treated AA increased with increasing thermal treatment temperature. At an initial fluoride concentration of 200 mg/L, the sorption capacity of AA700 was 3.67 times greater than that of UAA. The BET analysis showed that the specific surface area of UAA was about 2 times larger than that of AA700. The XRD analysis indicated that UAA was composed of both boehmite (AlOOH) and bayerite ($Al(OH)_3$) while AA700 was $Al_2O_3$. The reason that fluoride sorption capacity of AA700 increased despite of decrease in specific surface area compared to UAA could be attributed to the change of crystal structure. The kinetic sorption test showed that fluoride sorption to AA700 arrived at equilibrium after 24 h. The equilibrium test demonstrated that the maximum sorption capacity of AA700 was 5.70 mg/g. Additional batch experiments indicated that fluoride sorption to AA700 was the highest at pH 7, decreasing at both acidic and basic solution pHs. Also, fluoride sorption to AA700 decreased in the presence of anions such as phosphate, nitrate, and carbonate. This study demonstrated that thermal treatment of AA at high temperature could increase its sorption capacity for fluoride.

Effect of Extended Field of View on Measurements of Standardized Uptake Value in PET/CT (PET/CT검사에서 CT의 확대 유효시야 적용이 표준화섭취계수에 미치는 영향)

  • Park, Soon-Ki;Nam, Ki-Pyo;Kim, Kyeong-Sik;Shin, Sang-Ki
    • The Korean Journal of Nuclear Medicine Technology
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    • v.13 no.1
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    • pp.82-85
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    • 2009
  • Purpose: The purpose of this study was to evaluate the effect of extended CT field of view (FOV) on PET/CT of Standardized uptake value (SUV) when imaging extends beyond the CT FOV. Materials and Methods: CT images were reconstructed at different FOV sizes (500 and 700 mm). Two sets of CT images were reconstructed from the CT projection data by using two FOV sizes. Twenty patients were used in this study. PET images were reconstructed using attenuation maps with 500 mm CT FOV and 700 mm extended CT FOV images. Region of interests (ROIs) drawn on the PET images. In addition, twenty patients' PET images reconstructed by 500 mm CT FOV and 700 mm extended CT FOV were compared with $SUV_{max}$. Results: When using attenuation maps with 700 mm extended CT FOV, the $SUV_{max}$ analysis of liver (p=0.000), lung (p=0.007), mediastinum (p=0.001) were statistically significant. Conclusions: 700 mm extended CT FOV helps to recover the true activity distribution in the PET emission data. In addition, 700 mm extended CT FOV has affected SUV measurement of liver, lung, mediastinum.

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Effect of pH on the Synthesis of $LiCoO_2$ with Malonic Acid and Its Charge/Discharge Behavior for a Lithium Secondary Battery

  • Kim, Do Hun;Jeong, Yu Deok;Kim, Sang Pil;Sim, Un Bo
    • Bulletin of the Korean Chemical Society
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    • v.21 no.11
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    • pp.1125-1132
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    • 2000
  • The pH effect of the precursor solution on the preparation of $LiCoO_2$ by a solution phase reaction containing malonic acid was carried out. Layered $LiCoO_2$ powders were obtained with the precursors prepared at the different pHs (4, 7, and 9) and heat-treated at $700^{\circ}C(LiCoO_2-700)$ or $850^{\circ}C(LiCoO_2-850)$ in air. pHs of the media for precursor synthesis affects the charge/discharge and electrochemical properties of the $LiCoO_2electrodes.$ Upon irrespective of pH of the precursor media, X-ray diffraction spectra recorded for $LiCoO_2-850$ powder showed higher peak intensity ratio of I(003)/I(104) than that of $LiCoO_2-700$, since the better crystallization of the former crystallized better. However, $LiCoO_2$ synthesized at pH 4 displayed an abnormal higher intensity ratio of I(003)/I(104) than those synthesized at pH 7 and 9. The surface morphology of the $LiCoO_2-850$ powders was rougher and more irregular than that of $LiCoO_2-700$ made from the precursor synthesized at pH 7 and 9. The $LiCoO_2electrodes$ prepared with the precursors synthesized at pH 7 and 9 showed a better electrochemical and charge/discharge characteristics. From the AC impedance spectroscopic experiments for the electrode made from the precursor prepared in pH 7, the chemical diffusivity of Li ions (DLi+) in $Li0.58CoO_2determined$ was 2.7 ${\times}$10-8 $cm^2s-1$. A cell composed of the $LiCoO_2-700$ cathode prepared in pH 7 with Lithium metal anode reveals an initial discharge specific capacity of 119.8 mAhg-1 at a current density of 10.0 mAg-1 between 3.5 V and 4.3 V. The full-cell composed with $LiCoO_2-700$ cathode prepared in pH 7 and the Mesocarbon Pitch-based Carbon Fiber (MPCF) anode separated by a Cellgard 2400 membrane showed a good cycleability. In addition, it was operated over 100 charge/discharge cycles and displayed an average reversible capacity of nearly 130 mAhg-1.

Phase stability of TiO2 synthesized by Sol-gel Method at various pH and calcination temperatures (졸-겔 방법으로 합성된 TiO2 상안정성에 대한 pH 및 열처리 온도의 영향)

  • Lee, Jae-Yeon;Kim, Yong-Jin;Kim, Dae-Sung;Shin, Hyo-Soon;Nahm, Sahn;Chun, Myoung-Pyo
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.31 no.4
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    • pp.166-173
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    • 2021
  • The rutile phase of TiO2 forms a stable phase at high temperatures compared to anatase phase, but the stable temperature range of anatase changes depending on the synthesis conditions. In this study, nano-sized TiO2 was synthesized by the Sol-gel method using TiOSO4 and a mixed solvent of ethanol and distilled water, and the phase change of anatase and rutile according to pH and heat treatment temperature was investigated. Changes in the ratio of anatase and rutile were observed by changing the pH (3, 5, 7, 9) and heat treatment temperature (500, 600, 700, 800, 900℃) conditions of the prepared TiO2. As a result of observing these changes through XRD and FE-SEM analysis, anatase TiO2 at 500℃ and rutile TiO2 at 900℃ were observed. According to the pH, at these intermediate temperatures of 600, 700 and 800℃, the ratio of anatase and rutile changes. At 700℃, it was concluded that pH = 3~5 had a larger ratio of anatase TiO2, and pH = 7~9 had a larger ratio of rutile TiO2.

Dependence of the Diode Characteristics of ZnO/b-ZnO/p-Si(111) on the Buffer Layer Thickness and Annealing Temperature (버퍼막 두께 및 버퍼막 열처리 온도에 따른 ZnO/b-ZnO/p-Si(111)의 전기적 특성 변화 및 이종접합 다이오드 특성 평가)

  • Heo, Joo-Hoe;Ryu, Hyuk-Hyun
    • Journal of the Korean Vacuum Society
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    • v.20 no.1
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    • pp.50-56
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    • 2011
  • In this study, the effects of ZnO buffer layer thickness and annealing temperature on the heterojunction diode, ZnO/b-ZnO/p-Si(111), were reported. The effects of those on the structural and electrical properties of zinc oxide (ZnO) films on ZnO buffered p-Si (111) substrate were also studied. Structural properties of ZnO thin films were studied by X-ray diffraction and I-V characteristics were measured by a semiconductor parameter analyzer. ZnO thin films with 70 nm thick buffer layer and annealing temperature of $700^{\circ}C$ showed the best c-axis preferred orientation. The best electrical property was found at the condition of buffer layer annealing temperature of $700^{\circ}C$ and 50nm thick ZnO buffer layer (resistivity: $2.58{\times}10^{-4}[{\Omega}-cm]$, carrier concentration: $1.16{\times}1020[cm^{-3}]$). The I-V characteristics for ZnO/b-ZnO/p-Si(111) heterojunction diode were improved with increasing buffer layer thickness at buffer layer annealing temperature of $700^{\circ}C$.

Identification of Phellinus linteus by Comparison of Colony Shapes and Using PCR techniques (목질진흙버섯(Phellinus linteus)의 균총형태 비교 및 PCR 기법을 이용한 동정)

  • Kong, Won-Sik;Kim, Dong-Hyun;You, Chang-Hyun;Kim, Young-Ho;Kim, Kyung-Soo;Kim, Kwang-Ho
    • The Korean Journal of Mycology
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    • v.26 no.4 s.87
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    • pp.466-477
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    • 1998
  • Twenty-two Phellinus strains were characterized using colony morphologies and polymerase chain reaction (PCR) to divide into Phellinus linteus. There were some differences in mycelial growth and colony shapes among the strains when they were grown on various media such as PDA, MCM, MEA and YM. Phellinus linteus was slowly growing, formed golden-yellow colony, and produced blue pigment on PDA media. When the regions of internal transcribed spacer (ITS) were amplified from ribosomal RNA (rRNA) coding genes of P. igniarius and P. linteus strains by means of PCR, two types of band (700 bp and 800 bp) were appeared, respectively. For the amplified intergenic region I (IGRI), P. igniarius strains showed a different band among 500, 600, 700 and 800 bp according to the strains, whereas P. linteus strains did one specific band of 700 bp. By polymorphism analysis after digesting the amplified products with 6 different restriction enzymes, a band specific to P. linteus was generated when the products for ITS region were digested with HaeIII, suggesting that the enzyme digestion could provide effective method to distinguish between P. igniarius and P. linteus. And also, the analysis of genetic relationship showed that the genetic similarities were 89% and 95% in P. igniarius and P. linteus strains, respectively. Random amplification polymorphic DNA (RAPD) analysis using multiple primer sets and arbitrarily primed PCR (AP-PCR) with ITS3 primer could also result in a reproducible way to identify P. linteus strains.

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Effect of Low Temperature for the Breaking Dormancy of Primula sieboldii, P. modesta var. fauriae and Disporum smilacinum (저온처리 기간이 앵초, 설앵초 및 애기나리의 휴면타파에 미치는 영향)

  • Hong Su-Young;Yoo Dong-Lim;Kim Su-Jeong;Nam Chun-Woo;Ryu Seung-Yeol;Suh Jong-Taek
    • Korean Journal of Plant Resources
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    • v.19 no.1
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    • pp.45-49
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    • 2006
  • This study has been conducted to find out the possibility of the winter production for pot cultured plants, such as P. sieboldii, P. modesta var. fauriae and D. smilacinum in highland, 800 meter above sea level by early breaking dormancy. Low temperatures for breaking dormancy were treated for 300, 500, 700 and 900 hours under $5^{\circ}C$ in incubators and, then these plants were moved to greenhouse controlled at $10^{\circ}C$ of night temperature. Results showed that P. modesta var. fauriae and D. smilacinum, and P. smilacinum required more than 500 hours and 700 hours of low temperature $5^{\circ}C$ for breaking dormancy, respectively.

Structural Changes of Hydrous Titania by Heat-Treatment (열처리에 의한 함수 티타니아의 구조적 변화)

  • Choe, Byeong-Cheol;Lee, Mun-Ho
    • Korean Journal of Materials Research
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    • v.4 no.4
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    • pp.477-482
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    • 1994
  • Structural changes of hydrous titania by heat-treatment was investigated with XRD, TEM, FT-IR, Rarnan spectral analyses. The hydrous titania was derived from a mixed solution of titanium tetrachloride and hydrogen peroxide at $30^{\circ}C$ and pH of 9.0. The precipitate was an anatase form of titania with less-developed crystalline structure. With increasing annealing temperature ranglng up to $700^{\circ}C$, the crystallinity of anatase increased, and the particles were grown at high temperature. The rutile form of titania was developed from the anatase at $700^{\circ}C$.

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