• Journal of The Korean Society of Grassland and Forage Science
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• v.40 no.4
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• pp.216-220
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• 2020

This field evaluated the effect of different yields on the drying rate of Italian ryegrass ("Kowinearly", Lolium multiflorum Lam.) hay during the spring season. The experiment was performed at Hwaseong, 2015. The different yields of the plot were selected by growth and development check. Three treatments were 35 t/ha(Y1), 45 t/ha(Y2), and 60 t/ha(Y3). After harvest with conditioner, it was conducted tedding 1/day. It was investigated patterns for changes in moisture content. The changes in moisture content were respectively Y1 from 78.9% to 14.4%, Y2 from 79.1% to 18.3%, and Y3 from 77.9% to 25.5% for 5days. It was no different significantly for changes in moisture content by yields from 1st day ~ 2nd day, But It was different significantly from 3rd day to 5th day. the content of neural detergent fiber(NDF) and acid detergent fiber(ADF) were respectively about 34%, 59%, regardless of yields (P>0.05). Crude protein of Yields Y1, Y2, and Y3 were respectively 6.9%, 8.7%, and 9.4% (P<0.05). The result of this study that the higher yields, the slower it dried. The Quantity is a factor to effect on drying rate.

• Journal of Apiculture
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• v.34 no.3
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• pp.245-254
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• 2019

We investigated the anti-tumor effects and molecular mechanism of Brazil, China and Korean regional propolis on HeLa ovarian cancer cell line. Each propolis extracts was prepared by ethanol extraction method. Cytotoxicity of propolis extracts was determinated by EZ-cytox cell viability assay. To necessity of anti-tumor effect and molecular mechanism of propolis, we must be adjusting propolis concentration. Due to 100 ㎍/mL of propolis extract were reduced cell viability to less than 50%, we adjusted all of propolis concentration to 100 ㎍/mL. By Western blotting analysis, we confirmed that anti-tumor mechanism of Brazil, China and Korea regional propolis has significantly difference. All of propolis was activated apoptosis related molecules such as PARP, caspase-3. However, cell proliferation signaling molecules including Akt1, ERK and Bcl-2 were reduced the protein expression level. Especially, the expression of tumor suppressor protein p53 was significantly increased in propolis-treated group such as Gyeonggi, Chungbuk, Chungnam, Jeonbuk, Gyeongnam and China. The phosphorylation of Bax which as apoptosis indicator was appeared in propolis-treated group such as Gyeonggi, Gangwon, Chungnam, Gyeongbuk, China. In this results showed that the regional propolis has completely different mechanism in anti-tumor. Thus, propolis extracts may be useful source of functional materials on anti-cancer and it will be able to choose the suitable propolis for cancer therapy by analyzing individual characteristics.

• Journal of Acupuncture Research
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• v.37 no.2
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• pp.128-135
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• 2020

Background: Classical acupuncture is being used in the treatment of rheumatoid arthritis (RA). To explore the biological response to acupuncture, a network-based analysis was performed on gene expression data collected from an animal model of RA treated with acupuncture. Methods: Gene expression data were obtained from published microarray studies on blood samples from rats with collagen induced arthritis (CIA) and non-CIA rats, both treated with manual acupuncture. The weighted gene co-expression network analysis was performed to identify gene clusters expressed in association with acupuncture treatment time and RA status. Gene ontology and pathway analyses were applied for functional annotation and network visualization. Results: A cluster of 347 genes were identified that differentially downregulated expression in association with acupuncture treatment over time; specifically in rats with CIA with module-RA correlation at 1 hour after acupuncture (-0.27; p < 0.001) and at 34 days after acupuncture (-0.33; p < 0.001). Functional annotation showed highly significant enrichment of porphyrin-containing compound biosynthetic processes (p < 0.001). The network-based analysis also identified a module of 140 genes differentially expressed between CIA and non-CIA in rats (p < 0.001). This cluster of genes was enriched for antigen processing and presentation of exogenous peptide antigen (p < 0.001). Other functional gene clusters previously reported in earlier studies were also observed. Conclusion: The identified gene expression networks and their hub-genes could help with the understanding of mechanisms involved in the pathogenesis of RA, as well understanding the effects of acupuncture treatment of RA.

• Journal of Aquaculture
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• v.16 no.4
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• pp.257-261
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• 2003

The effects of different stocking densities on the growth of juvenile abalone, Haliotis discus hannai and water quality in the recirculating system with rotating biological contactor, were assessed. The trials were conducted using total weight 32 g juveniles abalone for 60 days at stocking densities of 5, 10, 15 kg/$m^2$. The animals were fed sufficient amounts of the pellet diet of 30.4% protein during the experimental period. Daily feeding rate (DFR) and survival rate (SR) of 5 kg/$m^2$ showed 0.76% and 85.5% those were not significantly differed 10 kg/$m^2$ showing 0.75% and 96.0% (P>0.05). DFR and SR of 15 kg/$m^2$ showing 0.38% and 31.2% were significantly lower than 5 and 10 kg/m2 (P<0.05). Daily growth rate (DGR) and feed efficiency (FE) showed the highest as 0.19% and 24.2% in 5 kg/$m^2$ the lowest as 0.05% and 14.3% in 15 kg/$m^2$ (P<0.05). According to, these results was an inverse relationship between growth and stocking density. The optimum density of juvenile abalone from this experiment is 5 to 10 kg/$m^2$ in the recirculating system.

• Journal of the Korean Society of Food Culture
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• v.36 no.4
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• pp.362-372
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• 2021

The present study was undertaken to investigate the quality characteristics of Korean Woori black pig (KWP) bellies and loins by different slaughter weight (SW) groups. The loin and belly samples collected from KWPs with different body weights (50, 75, 90, 105, and 120 kg) at 24 h post-mortem were used in the present investigation. The samples were analyzed for quality traits, fatty acid profiles, and volatile flavor compounds. Results showed that the fat content of the loin (8.64%) and belly samples (46.78%) was significantly higher in the 120 kg SW group compared to those of other SW groups (p<0.05). However, a lower protein content (12.20-12.67%) was found in the belly cuts of the heavier SW groups (105-120 kg) compared to those of the lighter SW groups (p<0.05). The lowest cooking loss (24.34%) was found in the loin cuts of the 120 kg SW group (p<0.05). Both the loin and belly cuts were observed to be redder in color with increasing SW (p<0.05). Higher oleic acid (C18:1, n9) and total monounsaturated fatty acid content and lower linolenic acid(C18:3, n3) and total polyunsaturated fatty acid content were observed in both cuts of the 120 kg SW group (p<0.05). Out of the flavor compounds identified, 11 and 17 compounds in the loin and belly, respectively, were associated with the SW. An increase in the SW resulted in increased concentrations of C18:1n9- and amino acid-derived flavor compounds. Overall, the meat samples of the heavier SW groups (120 kg) exhibited better quality and higher concentrations of volatile compounds associated with pleasant flavors. However, the meat of the 120 kg SW group also contained a much higher fat level (8.64 and 46.78% in the loin and belly, respectively) that may result in high trimming loss and hence a high rejection risk by consumers.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.2
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• pp.249-256
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• 2019

Objective: The present study aimed at comparing the nutritional composition and color traits between two meat types: Horse meat and pork from Korean native black pigs raised in Jeju Island. Methods: After slaughter 24 h, the longissimus dorsi samples were taken from left side carcasses of the 32-mo-old Jeju female breed horses and the 6-mo-old Korean native black pigs (n = 10 each). The samples were then placed into cool boxes containing ice packs and transported to the Laboratory of Meat Science where all visual fats and connective tissues were trimmed off and then the samples were ground. All the samples were analyzed for nutritional composition (proximate composition, minerals, vitamins, fatty acids, and amino acids) and color traits. Results: The horse meat contained significantly higher collagen, moisture and protein than the pork (p<0.05). The Jeju horse meat showed more desirable fatty acid profiles such as containing significantly lower saturated fatty acids (SFA), higher polyunsaturated fatty acids (PUFA) contents and PUFA/SFA ratios than the pork (p<0.05). Differences in concentrations of ten amino acids existed between the two meat types in which the horse meat had higher values for all these amino acids, total amino acids (20.33 g/100 g) and essential amino acids (10.06 g/100 g) than the pork (p<0.05). Also, the horse meat showed significantly higher concentrations of Fe (34.21 mg/100 g) and Cu (2.47 mg/100 g) than the pork (Fe, 17.42 mg/100 g and Cu, 1.51 mg/100 g) (p<0.05). All the vitamins detected showed statistical differences between the two meat types in which the horse meat had higher concentrations of vitamin B1 (25.19 mg/100 g), B2 (92.32 mg/100 g), B3 (2,115.51 mg/100 g), and B5 (67.13 mg/100 g) than the pork (p<0.05). Conclusion: Based on the results obtained in the study, it is concluded that the two meat types studied are rich in nutrients and the animal species strongly affected the nutritional values and color traits of the muscle tissues.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.29 no.2
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• pp.251-258
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• 2019

Objective: Chronic obstructive pulmonary disease(COPD) is characterized by persistent airflow limitations associated with chronic inflammatory response due to noxious particles or gases in the lung. Inflammation and oxidative stress are associated with COPD. The aim of this study was to evaluate the relationship among inflammation, oxidative stress, and airflow limitation severity in retired miners with COPD. Methods: The levels of serum high-sensitivity C-reactive protein(hsCRP) as a biomarker for inflammation, degree of reactive oxygen metabolites(dROMs) and biological antioxidants potential(BAP) in plasma as biomarkers for oxidative stress were measured in 211 male subjects with COPD. Degree of airflow limitation severity as determined by spirometry was divided into three grades grouped according to the classification of the Global Initiatives for Obstructive Lung Disease(GOLD)(1, mild; 2, moderate; $3{\leq}$, severe or more) using a fixed ratio, post- bronchodilator $FEV_1/FVC$ < 0.7. Results: Mean levels of dROMs significantly increased in relation to airflow limitation severity(GOLD 1, 317.8 U.CARR vs. GOLD 2, 320.3 U.CARR vs. GOLD $3{\leq}$, 350.9 U.CARR, p=0.047) and dROMs levels were correlated with serum hsCRP levels(r=0.514, p<0.001). Mean levels of hsCRP were higher in current smokers(non-smoker, 1.47 mg/L vs. smoker, 2.34 mg/L, p=0.006), and tended to increase with degree of airflow limitation severity(p=0.071). Mean levels of BAP were lower in current smokers(non-smoker, $1873{\mu}mol/L$ vs. smoker, $1754{\mu}mol/L$, p=0.006). Conclusions: These results suggest that inflammation and oxidative stress are related to airflow limitation severity in retired miners with COPD, and there was a correlation between inflammation and oxidative stress.

• Animal Bioscience
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• v.34 no.1
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• pp.93-101
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• 2021

Objective: Temperature could influence protein and amino acid deposition as well as gut microbiota profile and composition. However, the specific effects of ambient temperature on amino acids deposition and gut microbiota composition remain insufficiently understood. Methods: A total of 300 one-day-old Avian broilers were randomly divided into three groups and reared at high, medium, and low temperature (HT, MT, and LT), respectively. Breast muscle and fecal samples were collected for amino acid composition analysis and 16S rRNA gene sequence analysis. Results: Our data showed that compared to the MT group, there was a decrease of muscle leucine and tyrosine (p<0.05), as well as an increase of methionine in the HT group (p<0.05) and a decrease of serine in the LT group. Examination of microbiota shift revealed that at genus level, the relative abundance of Turicibacter and Parabacteroides was increased in the HT group (p<0.05) and that the relative abundances of Pandoraea, Achromobacter, Prevotella, Brevundimonas, and Stenotrophomonas in the LT group were higher than those in the MT group (p<0.05). In addition, there were substantial correlations between microbes and amino acids. In the HT group. Turicibacter was negatively correlated with aspartic acid and tyrosine, whereas Parabacteroides was positively correlated with methionine (p<0.05). In the LT group, there were multiple positive correlations between Achromobacter and arginine, isoleucine or tyrosine; between Prevotella and cysteine or phenylalanine; between Brevundimonas and cysteine; and between Stenotrophomonas and cysteine as well as a negative correlation between Stenotrophomonas and serine. Conclusion: Our findings demonstrated that amino acid content of breast muscle and intestinal microbiota profile was affected by different ambient temperatures. Under heat exposure, augmented abundance of Parabacteroides was correlated with elevated methionine. Low temperature treatment may affect muscle tyrosine content through the regulation of Achromobacter.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.2
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• pp.150-157
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• 2006

The aim of the present study was to investigate the effect of combined extract of safflower seed with herbs on the improvement of blood glucose, lipid peroxides, lipids in the plasma and liver of strpetozotocin-induced diabetic rats. Rats in the experimental group were orally administered with combined extract of safflower seed (100 mg, 200 mg/kg B.W.) with herbs (Ophiopogon japonicus Ker-Gaqler, Glycyrrhiza uralensis Fisch, Mori Folium, Poria cocos, Rehmannia glutinosa, Eriobtrya japonica, Aralia continentalis Kitagawa, Zizyphus jujuba var, Cornus officinalis, Paeonia suffruticosa, Trichosanthes kirilowii Maxim and Schizandra chinensis Baill) for 4 weeks. Body weight gain and food efficiency ratio were significantly lower in diabetic groups than those of control group. These were no protective effect of the supplementation of combined extract of safflower seed with herbs. Concentration of blood glucose was significantly higher in the diabetic groups than those in the control group. Blood glucose concentration was remarkably lower supplementation of combined extract of safflower seed (200 mg/kg B.W.) with herbs. There was no significant difference of plasma lipid peroxides among experimental groups, while liver lipid peroxides of diabetic group was significantly higher in control group. But supplementation of combined extract of safflower seed with herbs was induced markedly lower in liver lipid peroxides in diabetic rats. Diabetic groups had markedly higher levels in triglycerides, LDL-cholesterol and atherogenic index, while had lower HDL-cholesterol level. Triglyceride levels of plasma and liver were significantly lower with combined extract of safflower seed with herbs. But total cholesterol, phospholipid and free fatty acid were no differing significantly among experimental groups.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.2
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• pp.132-139
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• 1995

This experiment was canied out to select the soybean of high productivity suited for soiling. and growth characterisitcs, palatability, dry matter and crude protein yield of 10 cultivars of soybean have been evaluated at the experimental forage field. College of Natural Science of Kon-Kuk University in Chungju over three years (1 989-1 09 1 ). The results obtained are summarized as follows: JangBaek showed the tallest plant length from the 70th day to 80th day during cultivation period. and BaekUn showed the tallest at 90th day. PalDal among all cultivars showed the fastest growth rate and the strongest resistant lodging during cultivation period. The stem diameter of 70th day showed highly in order of PalDal and HwangKeum > BaekUn > KwangKyo and JangYeob, the 80th day showed highly in the order of TogYu > BaekCheon > JangBaek and 90th day showed highly in order of BangSa > BaekCheon > JangYeob. Stem hardness was highly increased with increasing of cultivation period, but no significant difference were found among the soybean cultivan. Especially, BaekUn and TogYu of 90th day showed very high as 17.9 and 17.6 kg/cm2, respectively. In the number of branch, PalDal was the lowest during cultivation period(70, 80 and 90th day), and 90th day showed lower two and three times than other soybeans(P<0.05). In the leaf rate, BaekCheon showed the highest according to cultivation period and the lowest in JangBaek. The fresh yield was tended to increase by increasing cultivation period at all cultivars except KwangKyo, but no significant differences were found among cultivars. Dry matter yield of JangYeob was the highest as 5, 861 kg/ ha at 70th day, and that of KwangKyo and TogYu was the highest as 7, 471 and 10, 603 kgha at 80th and 90th day, respectively. Relative palatability of 70th days showed very high at all soybean cultivars(81-100%), and that of 80th and 90th day showed the highest at TogYu, while showed the lowest at PalDal. In crude protein content of the 70th, 80th and 90th day, DanYeob, JangYeob and HwangKeum were the highest as 20.1, 17.9 and 20.4 percentage, respectively. Crude protein yield during cultivation period showed the highest at JangYeob(7Oth : 1, 068, 80th : 1, 211, 90th : 1, 882 kglha), but showed the lowest at BangSa(70th : 61 1, 80th : 814, 90th : 1, 078 kg/ha).