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Change of Volume of Isoflow in Pneumoconiosis Patients with Small Opacity (소음영 진폐증 환자에서의 등기류용량(Volume of Isoflow)의 변화)

  • Oh, Sang-Yong;Kim, Jee-Won;Jung, Chang-Young;Kim, Kyung-Ah;Yun, Im-Goung
    • Tuberculosis and Respiratory Diseases
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    • v.40 no.5
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    • pp.540-547
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    • 1993
  • Backgrounds: The measurement of volume of isoflow has been considered as a sensitive test for detecting small airway diseases showing normal pulmonary function in a routine pulmonary function test. To evaluate the functions of small airway among dust exposed workers, the changes of volume of isoflow were measured and its applicability of managing early stage pneumoconiosis patients was studied. Method: The subjects were 67 male, pneumoconiosis with small opacity and FEV1>80%, FEV1/FVC>75% in spirometry and the controls were 20 male, no dust-exposed office workers. The maximal epiratory volume curves after inhalation of indoor air and $He-O_2$ gas mixtures were measured and ${\Delta}V_{max50},\;{\Delta}V_{max75},\;V_{iso}V/VC$ between the dust exposed and control workers were compaired. Results: 1) There were no significant differences between two group in ${\Delta}V_{max50}$ and ${\Delta}V_{max75}$. But the ratio of $V_{iso}V/VC$ of the subjects was siginificantly higher than that of the control (p<0.01). This study confirms that $V_{iso}V/VC$ is a very useful index in early detection of small airway dysfunction. 2) The ratio of $V_{iso}V/VC$ of the subjects was signigicantly different between only smoker group and mixed group(smoker and nonsmoker). It suggestes that smoking is an important cousative factor of small airway dysfunction. 3) As the profusion of the chest X-ray increased, the rartio of $V_{iso}V/VC$ increased, but no significant difference of $V_{iso}V/VC$ was found between categories of pneumoconiosis. The categories of pneumoconiosis and small airway dysfunction may not be related. 4) No significant relationship was established between the duration of work and the ratio of $V_{iso}V/VC$. Conclusions : It is concluded that the measurement of $V_{iso}V/VC$ is useful to detect small airway dysfuction of early stage pnuemoconiosis patents with small opacities but showing normal pulmonary function in a routine pulmonary function test.

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Review on the Protected Areas Issues within Mid-Long Term National Plans for Territory and Environment of Korea; Focus on the "Biodiversity 2011-2020 Strategic Targets" and "Protected Areas Decision" (우리나라 국토 및 환경 분야 중장기 국가계획의 보호지역 관련 내용 고찰 - "생물다양성협약 2011~2020 전략목표" 및 "보호지역 결정문" 내용을 중심으로-)

  • Heo, Hag Young
    • Journal of Environmental Policy
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    • v.11 no.4
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    • pp.3-37
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    • 2012
  • In perspective of biodiversity conservation and protected areas (PAs), the aims of the study are to review the mid-long term national plans, which deal with national territory and environment in Korea, and to find out the way to improve this issue. Key issues were drawn by referring "Biodiversity 2011-2020 Strategic Targets" and "Protected Area Decision" in CBD CoP-10 and 7 National comprehensive or basic Plans were reviewed. Quoting Biodiversity 2011-2020 Strategic Target 5, "By 2020, the rate of loss of all natural habitats, including forests, is at least halved and where feasible brought close to zero, and degradation and fragmentation is significantly reduced", most of national plans included various methods such as "No Net Loss of Green", "No Net Loss of Wetlands", and so on. Regarding the target 11, "By 2020, at least 17% of terrestrial and inland water, and 10% of coastal and marine areas, ecologically representative and well connected systems of PAs and other effective area-based conservation measures, and integrated into the wider landscape and seascapes", 15% by 2015 was set up as a target of total PAs in Korea and 13% by 2015 or 2020 was set up as a target of coastal and marine PAs. CBD CoP-10 Decision X/31 (Protected Areas) invites parties to develop a national long-term action plan for the implementation of PoWPA and describes 10 issues that need greater attention. National action plan for the implementation of PoWPA doesn't be mentioned at any national plans even PoWPA. Regarding the 10 issues, most of issues were well reflected within various national plans, however there is still a need to improve the details and corelation between plans. Particularly, in terms of management effectiveness evaluation (MEE), there was no national plan to directly deal with MEE even though CBD invites parties to work towards assessing 60% of the total PAs by 2015. Based on the review results, below 4 items were suggested; (1)"The Comprehensive Plan of the National Territory" needs more attention on the Biodiversity Conservation and PAs, (2)Consider to establish "National PA System Plan" embedded into "the Comprehensive Plan of National Environment", (3)Establish a "National Action Plan for the implementation of PoWPA", (4)Improve the National Plans through linking with Biodiversity 2011-2020 Strategic Targets and relevant PA key issues.

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The Evaluation of IL-8 in the Serum of Pneumoconiotic patients (진폐증 환자에서의 혈청내 IL-8 농도)

  • Ahn, Hyeong Sook;Kim, Ji Hong;Chang, Hwang Sin;Kim, Kyung Ah;Lim, Young
    • Tuberculosis and Respiratory Diseases
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    • v.43 no.6
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    • pp.945-953
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    • 1996
  • Background : Many acute and chronic lung diseases including pneumoconiosis are characterized by the presence of increased numbers of activated macrophages. These macrophages generate several inflammatory cell chemoattractants, by which neutrophil migrate from vascular compartment to the alveolar space. Recruited neutrophils secrete toxic oxygen radicals or proteolytic enzymes and induce inflammatory response. Continuing inflammatory response results in alteration of the pulmonary structure and irreversible fibrosis. Recently, a polypeptide with specific neutrophil chemotactic activity, interleukin-8(IL-8), has been cloned and isolated from a number of cells including : monocytes, macrophages and fibroblasts. IL-1 and/or TNF-${\alpha}$ preceded for the synthesis of IL-8, and we already observed high level of IL-1 and TNF-${\alpha}$ in the pneumoconioses. So we hypothesized that IL-8 may be a central role in the pathogenesis of pneumoconiosis. In order to evaluate the clinical utility of IL-8 as a biomarker in the early diagnosis of pneumoconiosis, we investigated the increase of IL-8 in the pneumoconiotic patient and the correlation between IL-8 level and progression of pneumoconiosis. Method : We measured IL-8 in the serum of 48 patients with pneumoconiosis and 16 persons without dust exposure history as a control group. Pneumoconiotic cases were divided into 3 groups according to ILO Classification : suspicious group(n=16), small opacity group(n=16) and large opacity group(n=16). IL-8 was measured by a sandwich enzytne immunoassay technique. All data were expressed as the $mean{\pm}standard$ deviation. Results: 1) The mean value of age was higher in the small opacity and large opacity group than comparison group, but smoking history was even. Duration of dust exposure was not different among 3 pneumoconiosis groups. 2) IL-8 level was $70.50{\pm}53.63pg/m{\ell}$ in the suspicious group, $107.50{\pm}45.88pg/m{\ell}$ in the small opacity group, $132.50{\pm}73.47pg/m{\ell}$ in the large opacity group and $17.85{\pm}33.85pg/m{\ell}$ in the comparison group. IL-8 concentration in all pneumoconiosis group was significant higher than that in the comparison group(p<0.001). 3) IL-8 level tended to increase with the progression of pneumoconiosis. Multiple comparison test using Anova/Scheffe analysis showed a significant difference between suspicious group and large opacity group(p<0.05). 4) The level of IL-8 was correlated with the progression of pneumoconiosis(r=0.4199, p<0.05). Conclusion : IL-8 is thought to be a good biomarker for the early diagnosis of pneumoconiosis.

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Effect of Location, Year and Variety on Winter Cereal Forage Yield and Quality in the Southern Plateau of the Spain

  • Otal, J.;Quiles, A.;Quiles, A.;Perez-Sempere, J.I.;Ramirez, A.;Fuentes, F.;Hevia, M.L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.10
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    • pp.1416-1424
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    • 2008
  • The objective of this research was to study the production and quality of forage at three different times of the year (April, June and July) of six winter cereals in the southern plateau of the Iberian Peninsula. The cereals studied were Triticale (xTriticosecale wittm) cv. "Tritano", Oat (Avena sativa L.) cv. "Prevision" and cv. "Saia; Rye (Secale cereale L.) cv. "Giganton", Barley (Hordeum vulgare L.) cv. "Cameo" and cv. "Albacete". The study was carried out in three different locations and over three successive years of harvesting. The three variables considered were location, year and cereal. The % dry matter (DM), % crude protein (CP), % acid detergent fiber (ADF), % neutral detergent fiber (NDF) and % ash content were determined for the three sampling periods and the quality was calculated in milk forage units (MFU/kg DM), the production in kg DM/ha, MFU/ha and kg CP/ha. In all three sampling periods the quality of the cereals was significantly influenced only by the year and by species. However, for production of dry matter (kg DM/ha), milk forage units (MFU/ha) and gross protein (kg CP/ha) all three variables were of significant influence as were their interactions. In the April sampling, the species which showed a significantly higher production ($p{\leq}0.05$) was rye (1,693 kg DM/ha), which, along with its forage quality (16.56% CP, 0.886 MFU/kg DM) meant that the same occurred in MFU/ha and kg CP/ha. Significant differences between species were also found for the June sampling. The most productive cereal was again rye with 2,656 kg DM/ha, although its sharp fall in forage quality meant that barley cv Albacete (2,513 kg DM/ha) returned the highest production in forage units (1,934 vs. 1,951 MFU/ha) and barley cv. Cameo (2,413 kg DM/ha) in gross protein production (242 vs. 264 kg CP/ha). The significantly highest cereal production for July was barley cv. Albacete (4,923 kg DM/ha, 9.11% CP 0.722 MFU/kg DM). As a consequence of the results, we conclude that from the viewpoint of nutritional quality and production, rye is the most suitable for use in early spring in whatever year and location. However, barley cv. "Albacete" is the most appropriate for utilisation in later spring or early summer.

A Study on the Clothing Behavior of Elementary School Students in Connection with Sex-Role Identity and Home Enviornments (초등학교 아동의 성역할 정체감 및 가정환경변인에 따른 의복행동연구)

  • Jeon, Kyoung-Ran
    • Korean Journal of Human Ecology
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    • v.6 no.2
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    • pp.133-144
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    • 1997
  • Clothing is considered as a means of self-projection in the childhood and children show clear preference on their own favorite clothing on which their latent identity is reflected. Changing sex-role identity, in particular, in the modern complex society specially affects the clothing behavior of children. The purpose of this study is to investigate the clothing behavior of children in conjunction with sen-role identity and home environment variables. This study will provide fundamental information on the clothing behavior in the childhood and be of help in selecting appropriate clothing in accordance with the variation of sex-role identity. Necessary information for this study was obtained through a questionaire. 'Bem sex-role identity inventory' was used to investigate the effect of sex-role identity and the clothing behavior was studied in conjunction with the factors of conformity, dependence, aesthetics and interest. The subjects of investigation are composed of three hundred male and female elementary school students in the 5th and 6th grades. For the statistical analysis of the collected data, percentage(%), mean value(M), standard deviation(SD) and Pearson's correlation coefficient were calculated and t-test, $x^2$-test, and Duncan's multiple range test were performed. The results form this study are as follows : 1. Sex-role identity shows a little difference according to gender, order of a birth and standard of living. This small difference is shown to be statistically insignificant. 2. In clothing behavior in connection with the sex-role identity, statistically significant trend was not found in typical sex-role identity group. However, clothing behavior in modernistic sex-role identity group exhibits statistically significant results in the factors of dependence, aesthetics and interest at the level of P<.001. 3. As concerns the clothing behavior in connection with gender, boy students show statistically more significant tendency in aesthetics and interest than girls do. However, boy students show statistically more significant tendency in aesthetics than girls do in connection with the standard of living. Although the clothing behavior associated with the level of parents education, mother's age and existence of mother's job shows a little difference in mean values, statistically significant trend was not detected. 4. Clothing behavior of the children shows a meaningful correlation between the factors. 5. Through 3-dimensional variables analysis, it is found that there is no mutual interaction between gender, sex-role identity and standard of living on the clothing behavior. The results from MCA supplementary analysis reveal that there is difference according to the gender, sex-role identity and standard of living.

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Reproductive biology of common carp (Cyprinus carpio Linnaeus, 1758) in Lake Hayq, Ethiopia

  • Tessema, Assefa;Getahun, Abebe;Mengistou, Seyoum;Fetahi, Tadesse;Dejen, Eshete
    • Fisheries and Aquatic Sciences
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    • v.23 no.6
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    • pp.16.1-16.10
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    • 2020
  • This study was conducted in Lake Hayq between January and December 2018. The objectives of this study were to determine the growth, condition, sex ratio, fecundity, length at first sexual maturity (L50), and spawning seasons of common carp (Cyprinus carpio). Monthly fish samples of C. carpio were collected using gillnets of stretched mesh sizes of 4, 6, 7, 8, 10, and 13 cm and beach seines of mesh size of 6 cm. Immediately after the fish were captured, total length (TL) and total weight (TW) for each individual were measured in centimeters and grams, respectively, and their relationship was determined using power function. Length at first maturity (L50) was determined for both males and females using the logistic regression model. The spawning season was determined from the frequency of mature gonads and variation of gonadosomatic index (GSI) values of both males and females. Fecundity was analyzed from 67 mature female specimens. The length and weight relationship of C. carpio was TW = 0.015TL2.93 for females and TW = 0.018TL2.87 for males that indicate negative allometric growth in both cases. The mean Fulton condition factor (CF) was 1.23 ± 0.013 for females and 1.21 ± 0.011 for males. The value of CF in both cases was > 1 that shows both sexes are in good condition. Among the total 1055 C. carpio collected from Lake Hayq, 459 (43.5%) were females and 596 (56.5%) were males. The chi-square test showed that there was a significant deviation between male and female numbers from 1:1 ratio (χ2= 22, df = 11, P > 0.05) within sampling months. The length at first sexual maturity (L50) for females and males were 21.5 and 17.5 cm, respectively. Males mature at smaller sizes than females. The spawning season of C. carpio was extended from February to April, and the peak spawning season for both sexes was in April. The average absolute fecundity was 28,100 ± 17,462. C. carpio is currently the commercially important fish while Nile tilapia fishery has declined in Lake Hayq. Therefore, this baseline data on growth, condition, and reproductive biology of common carp will be essential to understand the status of the population of carp and design appropriate management systems for the fish stock of Lake Hayq, Ethiopia, and adjacent countries.

A study on the methodology for the automatic semantic web service composition problem (자동적인 시맨틱 웹 서비스 구성문제를 위한 방법론에 관한 연구)

  • Yang, Jin-Hyuk;Lee, Kang-Chan;Kim, Sung-Han;Min, Jae-Hong;Chung, In-Jeong
    • Proceedings of the Korea Information Processing Society Conference
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    • 2002.11c
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    • pp.2265-2268
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    • 2002
  • 인터넷 사용자들의 기하급수적인 증가와 웹 페이지의 폭발적인 증가로 인하여 정보공유를 위한 인터넷에서 효율적으로 원하는 정보를 발견하고 이용하기에 매우 힘든 상황에 처해있다. 따라서, 이러한 문제점들을 근본적으로 해결하기 위한 노력의 일환으로 기계가 이해하고 추론할 수 있는 시맨틱 웹이 등장하였다. 시맨틱 웹과 관련된 여러 가지 기술들 중 시맨틱 웹 서비스는 사용자에게 현재의 인터넷 환경에서 제공할 수 있는 서비스보다 향상된 고품질의 서비스를 제공하는 것을 목적으로 삼고 있다. 이러한 시맨틱 웹 서비스는 웹 서비스의 발견, 실행 및 구성으로 구성된다. 본 논문에서는 시맨틱 웹 서비스를 자동화하기 위한 노력의 일환으로서 시맨틱 웹에서 웹 서비스를 구성하는 문제에 대하여 언급한다. 시맨틱 웹 서비스 구성문제는 사용자의 요구사항을 충족시키기 위하여 다양한 웹 서비스들을 조합하는 문제이다. 그러나, WSFL, X-LANG 및 BPEL4WS 그리고, DAML-S와 같은 웹 서비스 구성문제에 대한 일련의 노력들에서는 사용자 요구사항에 대한 검증이나 서비스의 품질에 대한 사항들을 확인 및 제공할 수 있는 방법들이 없다. 따라서, 본 논문에서는 시맨틱 웹 서비스 구성문제와 관련된 상기와 같은 문제점들을 해결할 수 있는 방법론을 제시한다. 본 논문에서 제시된 방법론에서는 시맨틱 웹 구성문제를 제약만족문제로 변환함으로써 제약만족문제에 있어 늘리 알려진 다양한 알고리즘들을 활용할 수 있는 장점들이 있을 뿐만 아니라 사용자들의 요구사항에 대한 검증과 서비스의 품질을 확인할 수 있는 장점들이 있다.의 위상변화에 대한 적응성을 높일 수 있도록 한다. SQL Server 2000 그리고 LSF를 이용하였다. 그리고 구현 환경과 구성요소에 대한 수행 화면을 보였다.ool)을 사용하더라도 단순 다중 쓰레드 모델보다 더 많은 수의 클라이언트를 수용할 수 있는 장점이 있다. 이러한 결과를 바탕으로 본 연구팀에서 수행중인 MoIM-Messge서버의 네트워크 모듈로 다중 쓰레드 소켓폴링 모델을 적용하였다.n rate compared with conventional face recognition algorithms. 아니라 실내에서도 발생하고 있었다. 정량한 8개 화합물 각각과 총 휘발성 유기화합물의 스피어만 상관계수는 벤젠을 제외하고는 모두 유의하였다. 이중 톨루엔과 크실렌은 총 휘발성 유기화합물과 좋은 상관성 (톨루엔 0.76, 크실렌, 0.87)을 나타내었다. 이 연구는 톨루엔과 크실렌이 총 휘발성 유기화합물의 좋은 지표를 사용될 있고, 톨루엔, 에틸벤젠, 크실렌 등 많은 휘발성 유기화합물의 발생원은 실외뿐 아니라 실내에도 있음을 나타내고 있다.>10)의 $[^{18}F]F_2$를 얻었다. 결론: $^{18}O(p,n)^{18}F$ 핵반응을 이용하여 친전자성 방사성동위원소 $[^{18}F]F_2$를 생산하였다. 표적 챔버는 알루미늄으로 제작하였으며 본 연구에서 연구된 $[^{18}F]F_2$가스는 친핵성 치환반응으로 방사성동위원소를 도입하기 어려운 다양한 방사성의 약품개발에 유용하게 이용될 수 있을 것이다.었으나

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Within Field Distribution Pattern and Design of a Sampling Plan for Damaged Onions by the Onion maggot, Hylemya antiqua Meigen(Diptera: Anthomyiidae) (고자리파리에 의한 양파피해(被害)의 포장내(圃場內) 분포양식(分布樣式)과 피해량(被害量) 추정(推定)을 위한 표본추출(標本抽出) 계획(計劃))

  • Park, C.G.;Hyun, J.S.;Cho, D.J.;Lee, K.S.;Hah, J.K.
    • Korean journal of applied entomology
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    • v.24 no.1 s.62
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    • pp.29-33
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    • 1985
  • Every plant in $990m^2$ onion field was inspected for damages by the onion maggot. Maps were constructed every ten days to show which plants were infested and which were not from April 11 to May 21, 1984. The maps were sectioned into squares one of which contains 80 onion plants and the counts of damaged onions in each square were fitted to poisson and negative binomial distribution and tested by chi-square. We argue that the satisfactory fitness of the expected negative binomial $[P(x^2)>0.05]$ provided a useful description of the spatial distribution patterns of the damaged onions. Edge effect was tested by the differences of damage ratio and variance/mean ratio (${\sigma}^2/m$) between edge and center part. The result showed that the damage ratioes and variances of all the periods, ${\sigma}^2/m$ values after May 1 were greater in edge part than in center part. Again, the maps were sectioned into four blocks and the squares (sample units) were sectioned into quadrants. By application of the variance component technique, it was suggested that $2{\sim}8$ sample units for 5% sampling error and $1{\sim}2$ sample units for 10% error should be sampled randomly to estimate the damage ratio when $2{\sim}3$ quadrants were inspected.

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An Analysis of BMD Changes With Preoperative and Postoperative Premenopausal Breast Cancer Patient (폐경 전 유방암 환자의 치료 전.후 골밀도 변화 분석)

  • Kim, Su-Jin;Son, Soon-Yong;Choi, Kwan-Woo;Lee, Joo-Ah;Min, Jung-Whan;Kim, Hyun-Soo;Ma, Sang-Chull;Lee, Jong-Seok;Yoo, Beong-Gyu
    • Journal of radiological science and technology
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    • v.37 no.4
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    • pp.279-286
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    • 2014
  • The purpose of this study is to provide basic data of comparing BMD(bone mineral density) value of preoperative breast cancer patient and postoperative breast cancer patient due to bone loss with radiation/chemical therapy. The participants consisted of 254 breast cancer patients with BMD after having surgery and treatment from March 2007 to September 2013. Except for 84 patients with menopause or hysterectomy and we have analysed 171 patients. The BMD value(lumbar spine and femur) of before and after treatment from PACS by dure-energy X-ray absorptiometry was analyzed. First, we found variation of entire BMD and BMD according to treatment type, and analyzed detailed correlation by using marital status, number of children, presence of feeding, age of menarche, breast cancer therapy types as variable. Data was analyzed by using SPSS for Windows Program(version 18.0). BMD was decreased 7.1% in lumbar spine, 3.1% in femur respectively(p<.01). Also there is relatively high decrement($0.067g/cm^2$) in group who had just chemotherapy in femur(p<.05). There is decrement depend on marital status, number of children, presence of feeding, age of menarche, breast cancer therapy types but there was no statistical significance. The results show that BMD was decreased after treatment in premenopausal breast cancer patient, patient who had relatively high decrement need to be included high-risk group. As a result, aggressive prevention policy would be necessary.

Study of the Impact of Light Through the Vitamin $B_{12}$/Folate Inspection (Vitamin $B_{12}$/Folate 검사 시 빛의 영향에 대한 고찰)

  • Cho, Eun Bit;Pack, Song Ran;Kim, Whe Jung;Kim, Seong Ho;Yoo, Seon Hee
    • The Korean Journal of Nuclear Medicine Technology
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    • v.16 no.2
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    • pp.162-166
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    • 2012
  • Purpose : Vitamin $B_{12}$ and Folate are for anemia work-up which is well known for its sensitivity of light; the screening manual also specifies to be careful with light conditions. According to this, our laboratory minimized the exposure of light when inspecting the Vitamin $B_{12}$ and Folate, but the exposure cannot be wholly blocked due to other various factors such as when conducting specimen segregation. Thus, this inspection is to identify to what extent light can influence and whether the exclusion of light is mandatory during the Vitamin $B_{12}$/Folate test. Materials and Methods : We have conducted two experiments of identifying the extent of light's influence when conducting the Vitamin $B_{12}$/Folate test and also when specimens are under preservation. These experiments were progressed with various concentrations of patients' specimens which were requested to our hospital in March 2012. The first experiment is to verify the results on Vitamin $B_{12}$/Folate dependent on light exposure during the experiment. In the process, we have compared the results of light exposure/exclusion during the incubation process after the reagent division. The second experiment is about the impact of light exposure on the results on Vitamin $B_{12}$/Folate during the preservation. For 1, 2, 7 days the light on the specimen were wholly blocked and were preserved under $-15^{\circ}C$ temperature refrigeration. Then, we compared the results of light-excluded specimen and the exposed one. Results : When conducting first experiment, there were no noticeable changes in the Standard and specimen's cpm, but for Vitamin $B_{12}$, the average result of specimen exposed to light increased 7.8% compare to that of excluded one's. Furthermore, in the significant level 0.05, the significance probability or the p-value was 0.251 which means it has no impact. For Folate, the result being exposed to light decreased 5.4%, the significance probability was 0.033 which means it has little impact. For the second preservation, the result was dependent on the light exposure. The first day of preservation of Vitamin $B_{12}$, the clinical material exposed to light was 11.6%, second day clinical material exposed to light was 10.8%, seventh day clinical material exposed to light increased 3.8%, the significance probability of the $1^{st}$, $2^{nd}$, $7^{th}$ day is 0.372, 0.033, 0.144 respectively, and which indicates that the $1^{st}$ and $7^{th}$ day seems to have no impact. For Folate's case, the clinical material exposed to light has increased 1.4% but hardly had impact, $2^{nd}$ day clinical material being exposed to light was 6.1%, $7^{Th}$ day clinical material being exposed to light decreased 5.2%. The significance probability of Folate on the $1^{st}$, $2^{nd}$, $7^{th}$ day is 0.378, 0.037, 0.217 respectively, and the $1^{st}$ day and the $7^{th}$ day seems to have no impact. Conclusion : After scrutinizing the impact of light exposure/exclusion, Vitamin $B_{12}$ has no impact, while Folate seems to have no noticeable influence but light exclusion is recommended due to its significance probability of 0.033 when conducting experiment. During the preservation, the $2^{nd}$ day result depend on the light exclusion seems to have impact or influence. However, to consider the complication of the experimental process, the experiment including technical errors is predictable. Hence, it is likely to have no impact of light. Nevertheless, it is recommendable to exclude the light during the long preservation as per the significance probability (p-value) of $1^{st}$ and $7^{th}$ day has been diminished.

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