• Title/Summary/Keyword: P2P 모델

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Antioxidant Effect of Atractylodes macrocephala Koidzumi in DSS-induced Ulcerative Colitis Model (백출(白朮)의 항산화 효과가 DSS 유발 궤양성 대장염 모델에 미치는 영향)

  • Park, Seok Man;Lee, Se Hui;Jeong, Da un;Cho, Su-Jung;Shin, Mi-Rae;Park, Hae-Jin;Roh, Seong-Soo
    • The Korea Journal of Herbology
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    • v.37 no.1
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    • pp.19-29
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    • 2022
  • Objectives : Although the pharmacological effects of anti-inflammatory and antioxidant action of Atractylodes macrocephala Koidzumi water extract (AM) have been proven from many studies, reports on the antioxidant effect of AM on ulcerative colitis (UC) are scarce. Therefore, we aimed at evaluating the anti-oxidant effect of AM on the DSS-induced UC model. Methods : To induce ulcerative colitis, 8-week-old male Balb/c mice received 5% DSS in drinking water for 1 week. After 1 week of adaptation, mice were divided into four groups (n=8 each) for use as normal (Normal), DSS Control (Control), DSS + AM 100 mg/kg (AM100)-treatment, DSS + AM 200 mg/kg (AM200)-treatment. After 1 week of the experiment, the animals were sacrificed, and the extracted colon tissue was analyzed for protein through western blot. Results : As a result of confirming the macroscopic changes in colon tissues to confirm the therapeutic effects of AM, the decrease in colon length was suppressed in the AM treatment group compared to the control group. In addition, as a result of biochemical analysis, AM administration significantly reduced serum glutamic oxalacetic transaminase, glutamic pyruvate transaminase levels and tissue malondialdehyde levels. As a result of confirming the protein expression level through western blot, AM administration significantly decreased the expression of NADPH-related proteins such as NOX2, p22phox, and iNOS, but significantly increased the expression of SOD, catalase, and GPx-1/2. Conclusions : AM may improve DSS-induced UC in mice by modulating NADPH and antioxidant-related proteins. In conclusion, AM showed an antioxidant effect through the improvement of oxidative stress on UC.

Prediction of Carbon Accumulation within Semi-Mangrove Ecosystems Using Remote Sensing and Artificial Intelligence Modeling in Jeju Island, South Korea (원격탐사와 인공지능 모델링을 활용한 제주도 지역의 준맹그로브 탄소 축적량 예측)

  • Cheolho Lee;Jongsung Lee;Chaebin Kim;Yeounsu Chu;Bora Lee
    • Ecology and Resilient Infrastructure
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    • v.10 no.4
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    • pp.161-170
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    • 2023
  • We attempted to estimate the carbon accumulation of Hibiscus hamabo and Paliurus ramosissimus, semimangroves native to Jeju Island, by remote sensing and to build an artificial intelligence model that predicts its spatial variation with climatic factors. The aboveground carbon accumulation of semi-mangroves was estimated from the aboveground biomass density (AGBD) provided by the Global Ecosystem Dynamics Investigation (GEDI) lidar upscaled using the normalized difference vegetation index (NDVI) extracted from Sentinel-2 images. In Jeju Island, carbon accumulation per unit area was 16.6 t C/ha for H. hamabo and 21.1 t C/ha for P. ramosissimus. Total carbon accumulation of semi-mangroves was estimated at 11.5 t C on the entire coast of Jeju Island. Random forest analysis was applied to predict carbon accumulation in semi-mangroves according to environmental factors. The deviation of aboveground biomass compared to the distribution area of semi-mangrove forests in Jeju Island was calculated to analyze spatial variation of biomass. The main environmental factors affecting this deviation were the precipitation of the wettest month, the maximum temperature of the warmest month, isothermality, and the mean temperature of the wettest quarter. The carbon accumulation of semi-mangroves predicted by random forest analysis in Jeju Island showed spatial variation in the range of 12.0 t C/ha - 27.6 t C/ha. The remote sensing estimation method and the artificial intelligence prediction method of carbon accumulation in this study can be used as basic data and techniques needed for the conservation and creation of mangroves as carbon sink on the Korean Peninsula.

[ $^1H$ ] MR Spectroscopy of the Normal Human Brains: Comparison between Signa and Echospeed 1.5 T System (정상 뇌의 수소 자기공명분광 소견: 1.5 T Signa와 Echospeed 자기공명영상기기에서의 비교)

  • Kang Young Hye;Lee Yoon Mi;Park Sun Won;Suh Chang Hae;Lim Myung Kwan
    • Investigative Magnetic Resonance Imaging
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    • v.8 no.2
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    • pp.79-85
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    • 2004
  • Purpose : To evaluate the usefulness and reproducibility of $^1H$ MRS in different 1.5 T MR machines with different coils to compare the SNR, scan time and the spectral patterns in different brain regions in normal volunteers. Materials and Methods : Localized $^1H$ MR spectroscopy ($^1H$ MRS) was performed in a total of 10 normal volunteers (age; 20-45 years) with spectral parameters adjusted by the autoprescan routine (PROBE package). In all volunteers, MRS was performed in a three times using conventional MRS (Signa Horizon) with 1 channel coil and upgraded MRS (Echospeed plus with EXCITE) with both 1 channel and 8 channel coil. Using these three different machines and coils, SNRs of the spectra in both phantom and volunteers and (pre)scan time of MRS were compared. Two regions of the human brain (basal ganglia and deep white matter) were examined and relative metabolite ratios (NAA/Cr, Cho/Cr, and mI/Cr ratios) were measured in all volunteers. For all spectra, a STEAM localization sequence with three-pulse CHESS $H_2O$ suppression was used, with the following acquisition parameters: TR=3.0/2.0 sec, TE=30 msec, TM=13.7 msec, SW=2500 Hz, SI=2048 pts, AVG : 64/128, and NEX=2/8 (Signa/Echospeed). Results : The SNR was about over $30\%$ higher in Echospeed machine and time for prescan and scan was almost same in different machines and coils. Reliable spectra were obtained on both MRS systems and there were no significant differences in spectral patterns and relative metabolite ratios in two brain regions (p>0.05). Conclusion : Both conventional and new MRI systems are highly reliable and reproducible for $^1H$ MR spectroscopic examinations in human brains and there are no significant differences in applications for $^1H$ MRS between two different MRI systems.

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Nitroglycerin-Challenged Tc-99m MIBI Quantitative Gated SPECT to Predict Functional Recovery After Coronary Artery Bypass Surgery (니트로글리세린 투여 Tc-99m-MIBI 정량 게이트 심근SPECT를 이용한 관상동맥우회로술 후 심근 기능 회복 예측)

  • Lee, Dong-Soo;Kim, Yu-Kyeong;Cheon, Gi-Jeong;Paeng, Jin-Chul;Lee, Myoung-Mook;Kim, Ki-Bong;Chung, June-Key;Lee, Myung-Chul
    • The Korean Journal of Nuclear Medicine
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    • v.37 no.5
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    • pp.278-287
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    • 2003
  • Purpose: The performance of nitroglycerin-challenged Tc-99m-MIBI quantitative gated SPECT for the detection of viable myocardium was compared with rest/24-hour redistribution Tl-201 SPECT Materials and Methods: In 22 patients with coronary artery disease, rest Tl-20l/ dipyridamole stress Tc-99m-MIBI gated/24-hour redistribution Tl-201 SPECT were peformed, and gated SPECT was repeated on-site after sublingual administration of nitroglycerin (0.6 mg). Follow-up gated SPECT was done 3 months after coronary artery bypass graft surgery. For 20 segments per patient, perfusion at rest and 24-hour redistribution, and wall motion and thickening at baseline and nitroglycerin-challenged state were quantified. Quantitative viability markers were evaluated and compared;(1) rest thallium uptake, (2) thallium uptake on 24-hour redistribution SPECT, (3) systolic wall thickening at baseline, and (4) systolic wall thickening with nitroglycerin-challenge. Results: Among 100 revascularized dysfunctional segments, wall motion improved in 66 segments (66%) on follow-up gated myocardial SPECT after bypass surgery. On receiver operating characteristic (ROC) curve analysis, the sensitivity and specificity of rest and 24-hour delayed redistribution Tl-201 SPECT were 79%, 44% and 82%, 44%, respectively, at the optimal cutoff value of 50% of Tl-201 uptake. The sensitivity and specificity of systolic wall thickening at baseline and nitroglycerin-challenge were 49%, 50% and 64%, 65% at the optimal cutoff value of 15% of systolic wall thickening. Area under the ROC curve of nitroglycerin-challenged systolic wall thickening was significantly larger than that of baseline systolic wall thickening (p=0.004). Conclusion: Nitroglycerin-challenged quantitative gated Tc-99m-MIBI SPECT was a useful method for predicting functional recovery of dysfunctional myocardium.

Virus Inactivation during the Manufacture of a Collagen Type I from Bovine Hides (소 가죽 유래 Type I Collagen 생산 공정에서 바이러스 불활화)

  • Bae, Jung Eun;Kim, Chan Kyung;Kim, Sungpo;Yang, Eun Kyung;Kim, In Seop
    • Korean Journal of Microbiology
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    • v.48 no.4
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    • pp.314-318
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    • 2012
  • Most types of collagen used for biomedical applications, such as cell therapy and tissue engineering, are derived from animal tissues. Therefore, special precautions must be taken during the production of these proteins in order to assure against the possibility of the products transmitting infectious diseases to the recipients. The ability to remove and/or inactivate known and potential viral contaminants during the manufacturing process is an ever-increasingly important parameter in assessing the safety of biomedical products. The purpose of this study was to evaluate the efficacies of the 70% ethanol treatment and pepsin treatment at pH 2.0 for the inactivation of bovine viruses during the manufacture of collagen type I from bovine hides. A variety of experimental model viruses for bovine viruses including bovine herpes virus (BHV), bovine viral diarrhea virus (BVDV), bovine parainfluenza 3 virus (BPIV-3), and bovine parvovirus (BPV), were chosen for the evaluation of viral inactivation efficacy. BHV, BVDV, BPIV-3, and BPV were effectively inactivated to undetectable levels within 1 h of 70% ethanol treatment for 24 h, with log reduction factors of ${\geq}5.58$, ${\geq}5.32$, ${\geq}5.11$, and ${\geq}3.42$, respectively. BHV, BVDV, BPIV-3, and BPV were also effectively inactivated to undetectable levels within 5 days of pepsin treatment for 14 days, with the log reduction factors of ${\geq}7.08$, ${\geq}6.60$, ${\geq}5.60$, and ${\geq}3.59$, respectively. The cumulative virus reduction factors of BHV, BVDV, BPIV-3, and BPV were ${\geq}12.66$, ${\geq}11.92$, ${\geq}10.71$, and ${\geq}7.01$. These results indicate that the production process for collagen type I from bovine hides has a sufficient virus-reducing capacity to achieve a high margin of virus safety.

Angiotensin Converting Enzyme Inhibition and Antihypertensive Effects of Phyllostachys pubescens Culm Extracts in Spontaneously Hypertensive Rats (맹종죽 줄기 추출물을 투여한 본태성 고혈압 쥐(SHR)의 ACE 저해 활성 및 혈압 강하 효과)

  • Kim, Jung-Suk;Kim, Mi-Jeong;Park, Min-Hee;Ryu, Bog-Mi;Moon, Gap-Soon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.1
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    • pp.27-34
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    • 2008
  • This study investigated the anti-hypertensive effect of Phyllostachys pubescens culm extract (PCE) by examining its effects on renal angiotensin-converting enzyme (ACE) inhibition and blood pressure using the spontaneously hypertensive rat (SHR) system. Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured weekly for 8 weeks. Also, total antioxidant capacity and protein oxidation of tissues were examined by plasma Trolox Equivalent Antioxidant Capacity assay (TEAC) and hepatic protein carbonyl values, respectively. Twenty male SHR were randomly divided into four groups: PCE50, PCE100, and PCE500 (50, 100, and 500 mg of PCE per kilogram bodyweight daily, respectively), and control group. At week 2, the SBP in all PCE groups appeared to be significantly lower than the control (p<0.05), whereas the DBP were not different until week 4 (p<0.05). At week 8, SBP in the PCE500 was lower by 20% than the control. PCE groups considerably suppressed ACE dose-dependently compared with the control. Plasma TEAC and hepatic protein carbonyl values indicated increased antioxidative activity due to the PCE feed. No adverse effect was observed on the liver of SHR as there was no difference for the GOT and GPT values among the groups. Results of this study suggest that ACE inhibition may be one possible mechanism for the blood pressure lowering effect of PCE; thus, long term consumption of PCE may be beneficial in preventing high blood pressure along with the increased antioxidative status.

Effects of Silk Protein Hydrolysates on Blood Glucose and Serum Lipid in db/db Diabetic Mice (실크단백질 효소 가수분해물이 2형 당뇨 마우스의 혈당 및 혈청지질에 미치는 영향)

  • Shin, Mi-Jin;Park, Min-Jeong;Youn, Myung-Sub;Lee, Young-Sook;Nam, Moon-Suk;Park, In-Sun;Jeong, Yoon-Hwa
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.10
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    • pp.1343-1348
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    • 2006
  • This study was performed to investigate the effects of silk protein enzyme hydrolysates on blood glucose and serum lipid in db/db diabetic mice. Twelve week-old-male C57BL/KsJ db/db mice were divided into two groups: diabetic control group and 0.25% silk protein hydrolysates solution group, which were fed for 8 weeks. Body weight increased in the silk protein hydrolysates group compared with the diabetic control group. There were no differences in food and water intake between the diabetic control and the silk protein hydrolysates groups. The weight of liver increased in the silk protein hydrolysates group while that of kidney increased in the diabetic control group. The blood glucose level increased about 18.0% in the diabetic control group after 8 weeks while that in the silk protein hydrolysates group increased about 5.8%. Also, silk protein hydrolysates improved the glucose tolerance in C57BL/KsJ db/db mice. There was no difference in total cholesterol and non-HDL cholesterol concentration between the diabetic control and the silk protein hydrolysates group. Triglyceride concentration were lower in the silk protein hydrolysates group than in the diabetic control group (p<0.05) while HDL-cholesterol concentration were higher in the silk protein hydrolysates group than in the diabetic control group (p<0.05). This results suggest that administration of silk protein enzyme hydrolysates reduces significantly an increasing rate of 1]food glucose, decreases triglyceride, and increases HDL-cholesterol in C57BL/KsJ db/db mice.

Application and Effectiveness of a Preceptorship for the Improvement of Clinical Education (임상실습 교육개선을 위한 일 실습지도자 활용모델 (preceptorship model)의 적용 및 효과에 관한 연구 -암센타, 재활센타, 중환자실 실습을 중심으로-)

  • 이원희;김소선;한신희;이소연;김기연
    • Journal of Korean Academy of Nursing
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    • v.25 no.3
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    • pp.581-596
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    • 1995
  • Clinical practice in nursing education provides an opportunity for students, through the process of ap-plying theoretical knowledge to practice, and to learn nursing skills as well as being socialized into nursing and as such decrease the reality shock of actual nursing practice. Because of a shortage of nursing faculty, the job of achieving the objectives of the clinical practice had been turned over to the head nurses. This resulted in many problems, such as, unclear location of responsibilities and inadequate feedback from head nurses. Therefore this study was done to introduce and evaluate the use of preceptors as a way to minimize the above problems, and to maximize the achievement of the clinical practice objectives. Using an adaptation of Zerbe's (1991) three-tiered team model, clinical practice was done using a preceptor, a head nurse and a clinical instructor, each with different and well defined roles. The subjects of this study were 67 senior students of the College of Nursing of Y University in Seoul whose clinical practice in adult nursing was carried out between May 1, 1994 and December 8, 1994. There were 22 preceptors who had at least two years of clinical experience and who were recommended by their head nurses. They were given additional education on the philosophy and objectives of the College of Nursing, on communication skills, on the theory and practice of education, and on nursing diagnosis and education evaluation. The role of the preceptor was to work one-to-one with students in their practice. The role of the head nurse was to supervise and evaluate the preceptors. The role of the clinical instructor was to provide the education program for the preceptors, to provide ad-vice and suggestions to the preceptors and to maintain lines of communication with the college. With each of these roles in place, it was thought that the effectiveness and efficiency of the clinical practice could be increased significantly. To evaluate the effectiveness of the preceptorship, the three - tiered model, Lowery's Teacher Evaluation Opinion Form translated and adapted to Korea was used to measure student statisfaction. The Clinical Practice Compentency Evaluation Tool developed by Lee et ai was also used to measure student competencies. The results of this study are as follows 1. The satisfaction with clinical practice was higher with the introduction of the perceptors than it was before they were used. (t=-5.96, p=<.005) 2. The clinical practice competencies were higher with the introduction of the preceptors than it was before they were used(t=-5.l3, p<.005) 3. In order to analyze areas not measured by the quantitative tools additional analysis of the open questions was done. The results of this analysis showed that : 1) The students felt positive about their sense of security, confidence, handling of responsbility, and being systematic. They also felt positive about improvements in knowledge, opportunities for direct care, and socialization. 2) The students felt negative about the technical part of their role, lack of knowledge by the preceptor, unprofessional attitudes on the part of the preceptor, difficulty in the role of the professional nurse(student). 3) The preceptors felt positive about their responsibility, motivation, and relationship with the college. 4) The preceptors felt negative about their bur-den. Introduction of the preceptorship model will lead to change and improvement in the negative factors discussed above, solve problems in the present clinical education system, increase continuity in the education of the students, help with socialization of the students and motivation of the preceptors to up-grade their education and increase their confidence. These objectives must be obtained to further the development of professional nursing, and thus, making the preceptorship a reality is our job for the future.

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Genotype-by-Environment Interaction for Stickiness of Rice Cakes Using Glutinous Rice Cultivars in Different Environments (찰벼의 찰기에 대한 유전적 효과와 환경의 상호작용)

  • Yoon, Mi-Ra;Lee, Jeong-Heui;Cho, Jun-Hyun;Yang, Chang-Ihn;Lee, Jeom-Sig;Kwak, Jieun;Ahn, Eok-Keun;Kim, Mi-Jung;Kim, Sun-Lim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.62 no.4
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    • pp.317-324
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    • 2017
  • The purpose of this study was to provide basic data on the genetic and environmental effects of stickiness in glutinous rice varieties. In our study, we analyzed the genotype-by-environment ($G{\times}E$) interactions of the stickiness using six glutinous rice varieties under six environmental conditions. AMMI (Additive Main Effects and Multiplicative Interaction) analysis results showed that genotype (variety, G), environment (cultivation region, E) and $G{\times}E$ interaction were highly significant (P < 0.001). Among all the variations of stickiness for glutinous rice varieties, the environmental effect was 24.5%, the genetic effect was 37.1%, and the $G{\times}E$ interaction effect was 28.9%. From the AMMI analysis, the IPCA1 scores of Aranghangchal (G6, IPCA1: 3.85) and Hwaseonchal (G4, IPCA1: -5.24) was lower than other varieties. On the other hand, the Sangjuchal (G1, IPCA1: -61.23) and Boseogchal (G2, IPCA1: 41.21) were highly affected by environmental effects. In this study, there were large differences in stickiness according to region of cultivation. In the future, it is considered that a precise study should be carried out on the environmental factors that may increase the stickiness of glutinous rice varieties.

Analysis of Water Quality Improvement Effect by Securing Water Quality Characteristics and Flow Rate in the Geumho River (금호강 수질특성 및 유량확보에 따른 수질개선 효과 분석)

  • Kwak, Insoo;Choi, Boram;Jeon, Hyeryn;Kim, Sunae;Bae, Jaehyeong;Kim, Shin;Kim, Jungmin
    • Journal of Environmental Impact Assessment
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    • v.29 no.6
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    • pp.414-429
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    • 2020
  • For the management of rivers, the target water quality is set by establishing the total amount of water pollution and water environment basic plan. For Geumho river T-P has achieved the target water quality, but for BOD, COD, TOC the target water quality of the water environment basic plan has been exceed for the past five years. Therefore, the flow rate for satisfying the target water quality was simulated by analyzing the load, load density, and pollution contribution rate of the Geumho river using BOD, COD, TOC and by utilizing QUAL-MEV a one-dimensional water quality model. According to the analysis of the load, the BOD, COD and TOC all showed the highest levels at the Geumho C point at 9,832.2 kg/day 20,656.6 kg/day, and 15,545.1 kg/day. The load density was highest at 9.47 kg/day/㎢, 37.55 kg/day/㎢, 30.20 kg/day/㎢, and 17.19 kg/day/㎢, 39.14 kg/day/㎢ in Dalseocheon stream during the wet seasons and dry seasons. Pollution contribution rate was highest at about 25 percent for Palgeocheon stream during the wet season and about 50 percent for Dalseocheon stream during the dry season. In addition, the correlation analysis between organic materials showed in the main stream and tributaty of the Geumho river that COD-TOC was 0.8 or higherthan BOD-COD and BOD-TOC in both the wet seasons and dry seasons. And after surveying the total amount of water pollution and the target quality of the water environment basic plan at Geumho C, it was analyzed that an additional flow tate of 14 times and 22 times was needed as of April 2019 (3.46 ㎥/sec).