The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Antioxidant Effect of Atractylodes macrocephala Koidzumi in DSS-induced Ulcerative Colitis Model

백출(白朮)의 항산화 효과가 DSS 유발 궤양성 대장염 모델에 미치는 영향

  • Park, Seok Man (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Se Hui (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Jeong, Da un (Research Center for Herbal Convergence on Liver Disease, Daegu Haany University) ;
  • Cho, Su-Jung (Research Center for Herbal Convergence on Liver Disease, Daegu Haany University) ;
  • Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Park, Hae-Jin (DHU Bio Convergence Testing Center, Daegu Haany University) ;
  • Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 박석만 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이세희 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 정다운 (대구한의대학교 간질환한약융복합활용연구센터) ;
  • 조수정 (대구한의대학교 간질환한약융복합활용연구센터) ;
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 박해진 (대구한의대학교 DHU 바이오융복합시험센터) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2021.10.21
  • Accepted : 2022.01.25
  • Published : 2022.01.30
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Abstract

Objectives : Although the pharmacological effects of anti-inflammatory and antioxidant action of Atractylodes macrocephala Koidzumi water extract (AM) have been proven from many studies, reports on the antioxidant effect of AM on ulcerative colitis (UC) are scarce. Therefore, we aimed at evaluating the anti-oxidant effect of AM on the DSS-induced UC model. Methods : To induce ulcerative colitis, 8-week-old male Balb/c mice received 5% DSS in drinking water for 1 week. After 1 week of adaptation, mice were divided into four groups (n=8 each) for use as normal (Normal), DSS Control (Control), DSS + AM 100 mg/kg (AM100)-treatment, DSS + AM 200 mg/kg (AM200)-treatment. After 1 week of the experiment, the animals were sacrificed, and the extracted colon tissue was analyzed for protein through western blot. Results : As a result of confirming the macroscopic changes in colon tissues to confirm the therapeutic effects of AM, the decrease in colon length was suppressed in the AM treatment group compared to the control group. In addition, as a result of biochemical analysis, AM administration significantly reduced serum glutamic oxalacetic transaminase, glutamic pyruvate transaminase levels and tissue malondialdehyde levels. As a result of confirming the protein expression level through western blot, AM administration significantly decreased the expression of NADPH-related proteins such as NOX2, p22phox, and iNOS, but significantly increased the expression of SOD, catalase, and GPx-1/2. Conclusions : AM may improve DSS-induced UC in mice by modulating NADPH and antioxidant-related proteins. In conclusion, AM showed an antioxidant effect through the improvement of oxidative stress on UC.

Keywords

Acknowledgement

이 연구는 한국보건산업진흥원(KHIDI, HI15C00255)을 통해 보건복지부가 지원한 한약 연구개발 프로그램의 지원을 받았으며, 2021년도 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원(No. 2018R1A5A2025272)을 받아 수행된 연구이다.

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