• 생약학회지
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• 제35권3호통권138호
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• pp.215-228
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• 2004

The Korean folk medicine 'Jin Hae Cho Ip' has been used as a remedy for neuralgia and as an invigorating drug after a childbirth, etc in Korea. With regard to the botanical origin of 'Jin Hae Cho Ip', several species of Potentilla (Rosaceae) has been refired, but no pharmacognostical study has yet been performed in this regard. To clarify the botanical origin of the 'Jin Hae Cho Ip', the morphological and anatomical characteristics of the leaflets and petioles of Potentilla and Sibbaldia species growing in Korea, such as P. chinensis, P. cryptotaeniae, P. dickinsii, P. discolor, P. fragarioides var. major, P. freyniana, P. kleiniana, P. matsumurae, P. paradoxa, and S. procumbens, were compared. As a result, it was found that 'Jin Hae Cho Ip' was composed of the leaves of P. Chinensis and P. discolor.

• 식물분류학회지
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• 제48권2호
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• pp.134-142
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• 2018

The achene morphology of 21 taxa of Persicaria sect. Cephalophilon including the related taxa of Koenigia (Polygonaceae) was examined using stereo microscopy and scanning electron microscopy to evaluate its diagnostic values and taxonomic implications. The outlines of achene were narrowly ovoid to broadly ovoid in general, and elliptic achenes were observed in Koenigia taxa, with the achene shape being biconvex or trigonous. The size of the P. sect. Cephalophilon taxa ranged from $0.93-3.55{\times}0.80-2.31mm$ and the size of examined Koenigia taxa ranged from $1.10-1.84{\times}0.79-1.02mm$. The smallest achenes in P. sect. Cephalophilon were observed in P. humilis and the largest achenes were found in P. chinensis var. chinensis. Furthermore, infraspecific taxa of P. chinensis had relatively large achenes compared to other taxa within the section. Most taxa of P. sect. Cephalophilon had dull achenes, while four taxa had glossy achenes (P. capitata, P. runcinata var. runcinata, P. runcinata var. sinensis, and P. sinuata). Four types of surface patterns (smooth, tubercles, small pits, and irregular ridge) were observed, and the tubercles were divided into two subtypes according to the grouping and distribution pattern. The achene morphology of P. sect. Cephalophilon is described and compared, and its taxonomic implications are also discussed.

• Asian-Australasian Journal of Animal Sciences
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• 제20권6호
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• pp.872-879
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• 2007

A study was conducted to investigate the seasonal changes in nutrient composition of pasture, voluntary intake and digestibility of sheep grazing an introduced Leymus chinensis pasture located in western Jilin Province, China. The whole-plant of L. chinensis and the samples simulating ingestion by sheep (simulating sample) were collected in spring (May, 2004), summer (July, 2004), autumn (September, 2004) and the end of winter (April, 2005). The contents of gross energy (GE), organic matter (OM), crude protein (CP), neutral detergent fibre (NDF), acid detergent fibre (ADF) and alkanes were determined. Voluntary intake and apparent digestibility of the nutrients in each season were also examined using 6 mature Chinese Northeast Merino ewes (differed among the seasons) grazing on a paddock of 1 ha size. The apparent digestibilities of GE, dry matter (DM), OM, CP, NDF and ADF of L. chinensis were significantly greater (p<0.05) in spring and summer than in autumn and winter. Metabolizable energy (ME) content was 12.13, 11.62, 8.66 and 10.40 MJ/kg DM for L. chinensis in spring, summer, autumn and winter respectively, and the corresponding voluntary feed intakes were 91, 119, 59 and 58 g/d per kg metabolic weight ($LW^{0.75}$). The ME contents and DM intakes in autumn and winter were significantly lower than in spring and summer (p<0.05). The intake of L. chinensis pasture was sufficient to provide ME requirements for maintenance by the dry ewes in all the seasons, but inadequate for maintenance protein requirement in winter. (Supported by funds from National Basic Research Program of China, Grant No. 2007CB106800).

• 한국패류학회지
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• 제17권2호
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• pp.95-104
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• 2001

To determine optimal conditions for measurement of the clearance rate in feeding experiment of an intertidal bivalve Glauconome chinensis, effects of starvation, extent of mixing at subsampling, and initial prey concentration were assessed. Experiments were conducted separately for each condition with different treatments. Two-way ANOVAs showed that there were significant differences in clearance rates among different starvation periods (p<0.001), extents of mixing (p = 0.005), and prey concentrations (p < 0.001). Starvation for 1 or 2 days gave rise to 2 to 3-fold increase in the clearance rate. After starvation for 5 days, the clearance rate decreased seriously, implying loss of physiological status. It is suggested that animals should be fed during acclimation. The differences of the clearance rates between gentle and vigorous mixings were significant, but the differences were smaller than that among different incubation times. It was found that vigorous mixing is not necessary. The effect of initial prey concentration was great. However, optimal prey concentration could not be determined at any fixed value. Experiments with multiple concentrations of algal prey are recommended. Optimal incubation time for measurement of the clearance rate of G. chinensis was determined to be 2-4 hours.

• 생약학회지
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• 제43권3호
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• pp.239-242
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• 2012

This study was carried out to investigate the anti-malarial activity of Juniperus chinensis by in vitro and in vivo system using Plasmodium falciparum chloroquine-sensitive(3D7) and P. falciparum chloroquine-resistant(S20) strains. According to cytotoxicty test on NIH 3T3 cell, the ethanol extract(EtOH), ethylacetate(EtOAc) fraction and aqueous fraction possessed significant anti-malarial activity against both 3D7 and S20 strains at non-toxic concentrations(<100 /). In vitro assay, EtOAc fraction showed notable activity against 3D7 and S20 strains of P. falciparum with $IC_{50}$ values of $37{\pm}2{\mu}g/ml$ and $36{\pm}6{\mu}g/ml$. In animal test using P. falciparum infected human erythrocytes, the treatment of EtOAc fraction significantly inhibited parasitaemia in mice in a dose-dependent manner that is parasitaemia of 42%, 34% and 31% in doses of 10 mg/kg, 20 mg/kg and 40 mg/kg, respectively. The study provides data to support the medicinal importance of the J. chinensis.

• 생명과학회지
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• 제31권1호
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• pp.66-72
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• 2021

국내 유통되고 있는 새우젓은 다양한 작은 새우의 집합체로, 최근 어획량 감소로 인해 국내산 새우젓의 경우 수입산에 비해 두배 이상 높은 가격에 거래되고 있으며, 이에 중국 및 베트남 등으로부터 수입된 새우젓이 국내산으로 둔갑되어 판매되는 사례가 빈번하게 발생되고 있다. 본 연구에서는 새우젓 Acetes japonicus, A. chinensis (Korea, China), A. indicus (I, II), Palaemon gravieri 6종류에 대하여 PCR-RFLP 마커를 개발하였다. 새우젓에서 에탄올로 염을 제거한 후 gDNA를 추출하였고, 새우젓 COI 유전자의 특이 프라이머를 제작, PCR을 진행하여 519 bp의 증폭시켰다. 증폭된 PCR산물의 염기서열분석을 토대로 Acc I, Hinf I 두 가지의 제한효소를 마커로 선정하였고, 전기영동을 통해 결과를 확인하였다. Acc I을 처리한 결과, A. japonicus, A. chinensis (Korea), A. indicus (II)는 272, 247 bp로, P. gravieri는 271, 202, 46 bp, A. chinensis (China), A. indicus (I)는 519 bp로 band를 형성하는 것을 확인할 수 있었다. 또한 Hinf I을 처리한 결과로는 A. chinensis (Korea, China), P. gravieri는 519 bp로 잘리지 않은 것을 확인한 반면, A. japonicus와 A. indicus (I)는 2 band, A. indicus (II)는 3 band를 형성하는 것을 확인할 수 있었다. 따라서 위의 결과는 국내산과 수입산이 혼합되어있는 새우젓에 있어 보다 신속한 종판별을 할 수 있음을 시사한다.

• Natural Product Sciences
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• 제25권4호
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• pp.334-340
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• 2019

Cuscuta chinensis Lam. and Cuscuta japonica Choisy are parasitic plants. C. chinensis seeds were traditionally used for treatment of kidney and liver deficiencies. C. japonica seeds were used as tonic medicine to improve liver function and strengthen kidneys, treatment of high blood pressure, chronic diarrhea, and sore eyes. Cuscutae Semen are seeds of only C. chinensis in Korean Herbal Pharmacopoeia (K.H.P.). The developed HPLC-PDA method easily, accurately, and sensitively quantified using eight marker compounds [hyperoside (1), astragalin, (2), quercetin (3), kaempferol (4), chlorogenic acid (5), 3,4-di-O-caffeoylquinic acid (6), 1,5-di-O-caffeoylquinic acid (7), and 4,5-di-O-caffeoylquinic acid (8)]. In addition, the method may be used to distinguish seeds between C. chinensis Lam. and C. japonica Choisy. Furthermore, the result from the current study was applied to clarify samples between steam processed and unprocessed samples of C. chinensis by pattern analysis.

• Journal of Food and Nutrition Research
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• 제10권1호
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• pp.8-18
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• 2022

Schisandrae chinensis (Omija) is traditionally used as a food-based medicinal/pharmaceutical resources including antidiabetic agent in worldwide. In this study, the optimal combined formula of Omija extract and soybean mixture (OSM) were investigated for its effects on type 2 diabetes model expressed in in Vitro and in Vivo animal model. Using whole extracts, we confirmed inhibitory effects to α-glucosidase, α-amylase and the DPPH scavenging in Vitro and examined glucose tolerance in mice. The combined optimum formulation of OSM were significantly ameliorated type 2 diabetes-related. Furthermore, activation of p-Akt, p-AMPK, p-IRS and GLUT2 expression level is pivotal roles of in this anti-diabetic molecular mechanism on in Vivo without side effects. Therefore, these results suggest that OSM is good resources for improves of type 2 diabetes and its complications.

• 환경위생공학
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• 제22권1호
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• pp.29-35
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• 2007

This study was examined adsorption ability of heavy metal Hg(I), Pb(II), and U(Vl) ion use of fruit from schizandra chinensis, The fruits of schizandra chinensis sample used breaking into fragments $50{\sim}100$ mesh. The sample solution was mixed fruits of schizandra chinensis and heavy metal ion. Each heavy metal ion of into solution was quantum analysis with ICP-AES. As the result, each condition of maxium adsorption ability of heavy metal ion was high in the range of pH 5-7, adsorption time was about 15 minutes, and the optimum temperature was $100^{\circ}C$. The heavy metal ion was increased adsorption in order of increasing concentration and in ethanol solution better than in aqueous solution.

• 대한의생명과학회지
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• 제8권4호
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• pp.223-228
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• 2002

In vitro bioassays of aqueous extracts of 25 herbal species were performed by ovicidal activity against Strongvloides venuelensis. 500 mg of feces from Mongorian gerbil infected with S. venuelensis were placed into polyvinyl culture with extracts of herb and anthelmintic drugs in triplicates. Larval activity inside the polyvinyl bag was recorded microscopically at 12 hours, and every 24 hours thereafter for up to 72 hrs incubation. Eggs were not hatched within 72 hrs and degeneration was evident in the presence of extracts. The ovicidal activity characterized by the absence of L$_3$ in fecal cultures inside the polyvinyl bag. 1. Among 25 herbs, 12 herbs had ovicidal effects and caused degeneration of larvae in egg. S. angustifolia, I. helenium, A. korenum, P. tenuifolia, F. virridissima, A. asiatica, S. chinensis, A. tataricus, S. baicalensis, M. rimosa, S. glabra and Z. piperitum were the most ovicidal effect. However, P. thunbergiana, T. chinensis, C. flos, G. uralensis, C. obutusifolia, C. fortunei, A. koreana, P. grandiflorum, P. tenuifolia (Radix), P. mume and A. asphodelodes showed no ovicidal effects in vinyl bag culture. 2. Of 25 hrebs, 4 herbs had ovicidal and wormicidal effects, P. thunbergiana, C. flos, B. Striata and T. chinensis were characterized by degeneration of larvae.