• Journal of Korean society of Dental Hygiene
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• v.8 no.1
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• pp.59-71
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• 2008

This study was conducted to provide basic data required for the development of dental hygiene project for babies by investigating the relationship between mothers' dental hygiene activities and their socio-economic characteristics and the dental hygiene activities of babies. The survey was carried out with questionnaire to 306 babies of ages from 4 to 7 years and their mothers, from January 5 to January 25, 2007, in 4 kindergartens in Masan, Korea. The collected data was processed with SPSS v.12.0 for frequency analysis and ${\chi}^2$-test per item, to obtain following conclusions. 1. The frequency of toothbrushing of children is two times, accounted for 52.3%, they brush the teeth by them, and accounted for 52.3%, Fluorine toothpaste 68.3%. 2. The frequency of meal of children is 3 times, accounted for 93.5%, they ingestcariogenic snack 1 time, accounted for 45.4%, and they ingest protective and detergent snackfrom dental caries 2 times, accounted for accounted for 45.1%. 3. The shorter the period of mothers' duration of visit to dentists, the more experiences of fluorine coating and help from parent in toothbrushing had the children (pE.01, pE.05). 4. Children of housewife mothers received help from parent in toothbrushing and take teeth care and clean snack more than those of working mothers (pE.05, pE.01). 5. The children of working mothers had fluorine coating more than those of housewife mothers (pE.05). The fluorine coating, toothbrusing, protection and eating green food of babies show significant differences according to the frequency of mothers' visit to dentists and occupation.

• Korean Journal of Health Education and Promotion
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• v.13 no.2
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• pp.167-183
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• 1996

Human teeth vary widely in color. Practitioner and patients are concerned with preventing and correcting discolored or dark teeth to achieve and maintain stain-free, white teeth. Tooth brushing cannot alter tooth color but it can remove adhering films and stains. The esthetics of natural dentition can be improved by bleaching and this process can be applied to intrinsically and extrinsically stained teeth. The need for a brighter, more attractive smile has made rapid growth in the market for tooth whiteners. There is no doubt these products work as whiteners, at least on mild to moderate stains, but the safety of these products are unclear. In this experiment, the effect of tooth whitener application on the color and microhardness of extracted human enamel was measured. RMS, RMT and NWT were used as tooth whiteners, and tooth paste(ETQ) and hydrogen peroxide solution(HPO) were used as controls. 35 caries-free extracted human molars were embedded and polished with the exposed enamel diameter of 4 mm. The tooth whiteners and control agents were applied according to the manufacturers' instructions or clinically simulated procedures for eight weeks, and measurements were repeated every two weeks. Value(L＊) difference was measured using Differential Colorimeter(Model TC-6FX, Denshoku Co., Japan), and microhardness was measured using microhardness tester(Mitsuzawa Seiki Co., Japan). The results were as follows; 1. After application of agents for eight weeks, the Vickers hardness increased significantly in the ETQ, RMS and RMT application group(p〈0.01), and that decreased significantly in NWT application group(p〈0.01), but in HPO application group there was no significant change. The change in microhardness was greatest in NWT application group(p〈0.01). 2. After application of tooth whiteners and controls for eight weeks, the value change of toothpaste application group was significantly lower than those of other agents groups(p〈0.01), and there was no significant difference in value(L＊) change among tooth whitener groups(p〉0.01). 3. The application of tooth paste and paste type tooth whitener made gradual value change, but hydrogen peroxide gel type tooth whitener and hydrogen peroxide solution made rapid value change during initial application period.

• Journal of Korean society of Dental Hygiene
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• v.14 no.2
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• pp.173-179
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• 2014

Objectives : The purpose of this study is to provide the basic data for the development of oral health education program and to make schoolchildren practice the right oral health care behaviors. Methods : Data were collected from 259 elementary school students including 134 boys and 125 girls in Busan from November 26 to December 20, 2012. The groups were tooth brushing group and dental clinic group. All statistical analyses were analyzed by frequency analysis and chi-square test using SPSS 18.0 program. Results : School dental clinic group showed higher awareness level of fluoride and sealant effect of prevention(p=0.000). Toothbrushing facility group showed higher awareness level of brushing tooth(p=0.011). School dental clinic group showed higher awareness level of fluoride toothpaste(p=0.000). Both school dental clinic group and toothbrushing facility group showed higher awareness level of oral health education(p=0.001). School dental clinic group showed higher awareness level for tooth brushing method after education(p=0.000). Both school dental clinic group and toothbrushing facility group showed higher level of education. Conclusions : School dental clinic group showed higher level for oral health knowledge and toothbushing facility group showed high level for oral health behavior. Both School dental clinic group and toothbrushing facility group showed higher level of oral health education and tooth brushing method after education. Both school dental clinic group and toothbrushing facility group proved to promote oral health. Activation of school toothbrushing facility is very important to change the oral health workforce.

• Journal of dental hygiene science
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• v.15 no.6
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• pp.696-702
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• 2015

The purpose of this study was to evaluate the effect of oral health education with Q-scan for preschool children. The 59 children were divided into two groups, one with Q-scan and the other with dentiform, and they were evaluated by interview questionnaire and measurement of dental plaque index using Quigley-Hein index. Using SPSS ver. 18.0 for statistical analysis, paired t-test and Independent t-test were carried out for dental plaque index change and chi-square independence test for change between before and after oral health education. The result of this study revealed that the dental plaque index of the children showed significant decrease of 12% more in the experimental group (p<0.001). Both groups showed significant difference in knowledge about fluorine (p<0.05) and the percentage of correct answers showed significant increase in questions about bad food for teeth (p<0.05). In attitude toward oral health, the experimental group showed significant difference in every variable (p<0.05), and the control group showed significant difference in regular oral examination and frequency of toothbrushing (p<0.05). The experimental group showed significant result (p<0.05) with increase in the percentage of correct answers for the question about how to hold a toothbrush and decrease in the percentage of correct answers for the question about how to apply toothpaste, while the control group did not show significant difference in any variable. Therefore, Q-scan as an appropriate tool for motivation, provides effective educational methods in oral health education.

• Journal of Oral Medicine and Pain
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• v.22 no.1
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• pp.111-123
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• 1997

Peroxidase system은 구강내 고유의 항균체계로서 항세균물질인 $HOSCN/OSCN^-$을 생산하여 다양한 그램 양성 그리고 그램 음성 세균에 대해 살균 및 정균작용을 나타낸다. 본 연구는 peroxidase system을 함유한 치약을 사용한 후 건강한 대상자의 자극성 전타액내 생성되는 $HOSCN/OSCN^-$의 양을 측정하여 최대 생성시간을 결정하였다. 그 후 건강한 대상자에게 임의로 실험치약과 대조치약을 할당하여 2주간 사용하도록 한 후 $HOSCN/OSCN^-$ 최대 생성 시간에 타액을 채취하여 치약 사용전과 2주후의 타액분비량, pH, 점조도 그리고 S. mutans와 lactobacilli에 대한 항균효과를 비교하였다. 구강건조증 환자를 대상으로 한 두 번째 연구에서도 peroxidase system을 함유한 세치제를 사용한 후 생성되는 $HOSCN/OSCN^-$의 최대생성시간을 측정하였으며, peroxidase system을 함유한 세치제가 S. mutans와 lactobacilli에 미치는 영향을 양치전과 양치후 $HOSCN/OSCN^-$의 최대생성시간에 타액을 채취하여 비교하였다. 이와같은 실험을 통해 다음과 같은 결론을 얻었다. 1. 건강한 대상자의 자극성 전타액내의 $HOSCN/OSCN^-$의 농도는 양치후 30분 후 대조군에 비해 peroxidase systems을 함유한 세치제를 사용한 군에서 유의성있게 높게 나타났다 (p < 0.05). 2. 건강한 대상자의 자극성 전타액의 타액분비량, pH, 타액점조도는 peroxidase system을 함유한 세치제를 2주간 사용한 후에도 유의한 변화를 나타내지 않았다. 3. 2주간 실험치약을 사용한 후 건강한 대상자의 자극성 전타액내 S. mutans와 lactobacilli의 양은 peroxidase system을 함유한 세치제를 사용한 군에서 상대적으로 낮게 나타났으나 통계학적 유의성은 없었다(p = 0.206 : lactobacilli, p = 0.944 : S. mutans). 4. 구강건조증 환자의 자극성 전타액내의 $HOSCN/OSCN^-$의 농도는 양치후 30분(p < 0.01)과 60분(p < 0.05)에서 대조군에 비해 peroxidase system을 함유한 세치제를 사용한 군에서 유의성있게 높았다. 5. 구강건조증 환자의 양치 후 30분에 채취한 자극성 전타액내의 S. mutans와 lactobacilli의 양은 peroxidase system을 함유하거나(p < 0.01) 함유하지 않은(p < 0.05) 치약을 사용한 군 모두에서 양치전에 비해 유의성있게 감소하였다. 6. 양치후 30분에 채취한 구강건조증 환자의 자극성 전타액내 S. mutans 양은 peroxida system을 함유한 세치제를 사용한 군에서 대조군에 비해 유의성있게 낮았다(p < 0.05). 7. 양치후 30분에 채취한 구강건조증 환자의 자극성 전타액내 lactobacilli양은 peroxidase system을 함유한 세치제를 사용한 군에서 대조군에 비해 상대적으로 낮게 나타났으나(p = 0.067) 통계학적 유의성은 없었다.

• Journal of dental hygiene science
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• v.9 no.4
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• pp.381-385
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• 2009

This study carried out to provide basic educational material of primary school children to improve the children's oral health by oral health checkup and survey over 215 students in the first grade and the fourth grade at the primary school in Hwaseong city. The collected data were analyzed by SPSS Win 12.0 program 1. The rate of students who have decayed permanent teeth was 57.2%, and there seemed to be a significant difference between the two genders showing 61.6% for male students and 53.3% for female students. Also 1st grade showed 56.0% and 4th grade 58.1% respectively. 2. The male students who claimed 'bleeding gum' against questions about oral illness experiences were 15.7% whereas female students were 28.0%. There was a significant difference(p<0.05) by the gender. 3. The 4th grade students who said 'Toothache while eating cold food' about questions of oral symptoms appeared as 19.4% and 1st grade students were 8.8%. Also there was a significant difference(p<0.05) by the grade. 4. Regarding oral health improvement behavior, there were significant differences in frequency of toothbrushing a day, the number(p<0.05) of eating snacks a day and the number(p<0.01) of experience of visiting dental clinic(p<0.01). 5. About oral health behavior frequency of daily toothbrushing was found as twice(50.2%), twice of eating snacks a day(70.2%), using fluoride toothpaste 60.9% and visiting a dental clinic(60.0%), were shown as the highest numbers.

• Journal of Pharmaceutical Investigation
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• v.26 no.3
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• pp.221-232
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• 1996

An abrasive, calcium hydrogen phosphate dihydrate (DCPD), was synthesized in a Box-wilson experimental design by reactions between phosphoric acid and milk of lime, and calcium chloride and sodium phosphate solutions, and stabilized with TSPP and TMP. The optimum conditions for preparation of DCPD from phosphoric acid with milk of lime were such as; reaction temp.; $51.9^{\circ}C$, conc. of lime; 25.9%, conc. of phosphoric acd; 77.9%, drying temp.; $60.2^{\circ}C$ and final pH; 6.46. The physico-chemical and pharmaceutical properties of DCPD were showed as follows: glycerin absorption value(68 ml/100g), whiteness(99.5%), particle size(10.9 nm), pH(7.8), and set test(pass). XRD and SEM of DCPD indicated a monoclinic system crystallographically. $N_2$ adsorption isotherm curve by BET showed non porous type II form. The micromeritic parameters of DCPD showed that surface area was $3.27{\sim}4.6\;cm^{2}/g$ and pore volume, pore area and pore radius were negligible. The rheogram of the toothpaste containing DCPD showed pseudoplastic flow with yield value of 321, and thixotropic behavior forming hysteresis loop. These results meet the requirements as abrasive standard, and sythesized DCPD is expected as a good dental abrasive such as a high quality grade in practice.

• Journal of Korean society of Dental Hygiene
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• v.9 no.4
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• pp.670-684
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• 2009

Objectives : This research analyzed the general characteristics that closely affect the children's dental health and the mothers' dental health knowledge and dental health behavior to identify the correlation of the latter with the status of children's dental health in order to provide the basic data for the development of dental health business that would target children. Methods : Research subjects were selected arbitrarily from three nursery schools located in Busan metropolitan city, targeting 186 children between the ages of four to six and their mothers. The children were subjected to dental Inspection, and the status of their dental caries was studied whereas the mothers were subjected to the surveys on the general characteristics, dental health knowledge and dental health behavior. Results : 1. Level of mothers' dental health knowledge is higher when the mothers' educational level is higher(p=0.02) and when the household's monthly income is higher(p=0.009). 2. When the level of mothers' dental health knowledge is higher, children tended to brush their toothbrushing using proper method(p=0.025). Moreover, when the level of mothers' dental health knowledge is higher, they tended to take their children to dental clinic mostly for preventive measures than for treating cavity(p=0.023). 3. When the level of mothers' dental health knowledge is higher, children's dmft index was significantly low(p=0.02). When the mothers use fluoride-containing toothpaste, children's children' dmft index was even lower(p=0.02). 4. As the children tended to brush their teeth more often, dmft index was lower(p=0.003). When the reason that the children visited dental clinic was more to prevent, than to treat cavity, dmft index was even lower(p=0.000) Conclusions : When the above mentioned results are summarized, it is possible to know that the mothers' dental health knowledge and dental health behavior significantly affect children's dental health. Accordingly, it is necessary to develop maternal and child dental health program that factors in both the mothers and children in order to prevent children's dental caries and to increase their dental health, and continued care is required to discover and treat dental caries early on.

• Journal of Korean society of Dental Hygiene
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• v.15 no.4
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• pp.719-725
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• 2015

Objectives: The purpose of this study was to determine the toxic effects of sodium lauryl sulfate(SLS) in human keratinocyte HaCaT cells and mouse fibroblast NIH-3T3 cells. Methods: The effect of sodium lauryl sulfate(SLS) cell viability and proliferation were determined by WST-1 assay and changes shape of nucleus were evaluated by Hoechst staining under fluorescence microscopy. Additionally, observation of cell morphological changes under light microscopy. Results: SLS induced cytotoxicity and a marked apoptosis in both HaCaT and NIH-3T3 cell lines. With the result of the WST-1 assay, SLS induced the cytotoxicity of 0.005% and 0.0075%, 0.01% SLS for 24 h after HaCaT and NIH-3T3 cells in time and dose-dependent manner(p<0.005). SLS inhibited cell growth and caused apoptosis as evidenced by nuclear fragmentation and condensation. Thus, determination of the morphological changes to define apoptosis was visualized using inverted phase contrast microscopy. Conclusions: SLS had toxicity of the human keratinocyte cells and mouse fibroblast cells and this study will provide the basic data for the development of proper SLS concentration in dentifrice.

• Biomolecules & Therapeutics
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• v.24 no.1
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• pp.99-107
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• 2016

Triclosan is an antimicrobial or sanitizing agent used in personal care and household products such as toothpaste, soaps, mouthwashes and kitchen utensils. There are increasing evidence of the potentially harmful effects of triclosan in many systemic and cellular processes of the body. In this study, we investigated the effects of triclosan in the survivability of cultured rat neural stem cells (NSCs). Cortical cells from embryonic day 14 rat embryos were isolated and cultured in vitro. After stabilizing the culture, triclosan was introduced to the cells with concentrations ranging from $1{\mu}M$ to $50{\mu}M$ and in varied time periods. Thereafter, cell viability parameters were measured using MTT assay and PI staining. TCS decreased the cell viability of treated NSC in a concentration-dependent manner along with increased expressions of apoptotic markers, cleaved caspase-3 and Bax, while reduced expression of Bcl2. To explore the mechanisms underlying the effects of TCS in NSC, we measured the activation of MAPKs and intracellular ROS. TCS at $50{\mu}M$ induced the activations of both p38 and JNK, which may adversely affect cell survival. In contrast, the activities of ERK, Akt and PI3K, which are positively correlated with cell survival, were inhibited. Moreover, TCS at this concentration augmented the ROS generation in treated NSC and depleted the glutathione activity. Taken together, these results suggest that TCS can induce neurodegenerative effects in developing rat brains through mechanisms involving ROS activation and apoptosis initiation.