• 한국운동역학회지
• /
• 제16권3호
• /
• pp.43-51
• /
• 2006

The purpose of this study was to analyze kinematic variables during turing back kick motion of Taekwondo. The subjects of this study were the 4 skilled and 4 unskilled of male university player in respectively. The experiment of this study was used two 16mm high speed cameras and its speed 125 frames/s. Analysis of this data was three dimensional cinematography using KWON3D program package. The results were as following; 1. In the elapsed time, there was no significance difference statically between a skilled and unskilled group. But skilled group was more fast during the motion of I phase. And unskilled group was more fast during the motion of II phase so called force production section, which had an influence on Diechagi's velocity. 2. In the center of gravity of human body, the changing of it was $1.10{\pm}0.04m$, $1.12{\pm}0.03m$ of LFM(left foot movement) and $1.36{\pm}0.08m$, $1.39{\pm}0.09m$ of RKF(right knee flection), and $1.44{\pm}0.08m$, $1.42{\pm}0.09m$ of RFI(right foot impact). There was no significance difference statically between the two groups. 3. The velocity of heel on impact was 1.13m/s in the skilled group and 1.23m/s in the unskilled group, when each angle of knee was $110.4{\pm}10.9deg/s$, $114.8{\pm}28.4deg/s$. The maximum velocity of each performance was reached before the RKF, and the velocity and angle at impact along by two groups did not show any significant difference statically. 4. In the angular velocity of just RKF of lower leg, there was significance difference statically between the two groups(p<.05).

• 한국인터넷방송통신학회논문지
• /
• 제8권4호
• /
• pp.59-65
• /
• 2008

본 논문은 휴대용 멀티미디어를 위한 저전력 H.264 디블록킹 필터를 제안하였다. H.264 디블록킹 필터는 총 8개의 입력 픽셀에 대한 각각의 필터링 연산 과정을 필요로 하며, 각 필터링 과정에서 p, q 픽셀에 대해 공통 구조를 가지고 있다. 이 때 쓰이는 공통의 필터계수와 레지스터를 공유함으로써, 적은 게이트로 구현하였다. 또한 많은 연산을 필요로 하는 필터링 연산을 특정한 조건을 이용하여, 조건에 만족하면 일부 또는 전체의 필터링을 수행하지 않음으로써 저전력의 효율적인 구조를 설계할 수 있다. 제안한 H.264 디블록킹 필터 구조는 기존 논문들의 핵심 필터링부분과 비교하여 각각 33.31%와 10.85%의 게이트 감소효과를 나타내었다. 또한 본 논문의 전체 블록은 삼성 0.35um 표준 셀 라이브러리 공정을 사용하여 구현하였으며, 최대 동작 주파수는 108MHz, 최대 처리능력은 CCIR601 형식에서 33.03 frame/s이다.

• Steel and Composite Structures
• /
• 제16권5호
• /
• pp.491-506
• /
• 2014

Buckling-restrained braces (BRBs) have excellent hysteretic behavior while buckling-restrained braced frames (BRBFs) are susceptible to residual lateral deformations. To address this drawback, a novel self-centering (SC) BRB with Basalt fiber reinforced polymer (BFRP) composite tendons is presented in this work. The configuration and mechanics of proposed BFRP-SC-BRBs are first discussed. Then an 1840-mm-long BFRP-SC-BRB specimen is fabricated and tested to verify its hysteric and self-centering performance. The tested specimen has an expected flag-shaped hysteresis character, showing a distinct self-centering tendency. During the test, the residual deformation of the specimen is only about 0.6 mm. The gap between anchorage plates and welding ends of bracing tubes performs as expected with the maximum opening value 6 mm when brace is in compression. The OpenSEES software is employed to conduct numerical analysis. Experiment results are used to validate the modeling methodology. Then the proposed numerical model is used to evaluate the influence of initial prestress, tendon diameter and core plate thickness on the performance of BFRP-SC-BRBs. Results show that both the increase of initial prestress and tendon diameters can obviously improve the self-centering effect of BFRP-SC-BRBs. With the increase of core plate thickness, the energy dissipation is improved while the residual deformation is generated when the core plate strength exceeds initial prestress force.

• The Plant Pathology Journal
• /
• 제19권5호
• /
• pp.239-247
• /
• 2003

In Korea, a local soybean (Glycine max) genotype 56l. was found to be strongly resistant to a virulent bacterial strain of a Pseudomonas sp. SN239. Specific genes involved in the resistance of the soybean genotype 561 were identified and the pattern of gene expression against the Pseudomonas infection was analyzed using differential-display reverse transcription PCR (DDRT-PCR). More than 126 cDNA fragments representing mRNAs were induced within 48 hours of bacteria inoculation. Among them, 28 cDNA fragments were cloned and sequenced. Twelve differentially displayed clones with open reading frames had unknown functions. Sixteen selected cDNA clones were homologous to known genes of other organisms. Some of the identified cDNAs were pathogenesis-related (PR) genes and PR-like genes. These cDNAs included a putative calmodulin-binding protein; an endo-l,3-1,4-$\bate$-D-glucanase; a $\bate$-1,3-endoglucanase; a $\bate$-1,3-exoglucanase; a phytochelatin synthetase-like gene; a thiol protease; a cycloartenol synthase; and a putative receptor-like serine/threonine protein kinase. Among them, four genes were found to be putative PR genes induced significantly by the Pseudomonas infection. These included a calmodulin-binding protein gene, a $\bate$-1,3-endoglucanase gene, a receptor-like serine/threonine protein kinase gene, and pS321 (unknown function). These results suggest that the differentially expressed genes may mediate the strong resistance of soybean 561 to the strain SN239 of Pseudomonas sp.

• 한국강구조학회 논문집
• /
• 제29권5호
• /
• pp.369-376
• /
• 2017

본 논문은 ASCE 7-10를 적용한 비틀림 비정형 철골 모멘트 골조의 설계 및 평가를 수행한 동반연구(I 내진설계)의 후속 연구이다. 본 연구에서는 철골 모멘트 골조의 비틀림 비정형 발생 여부와 ASCE 7-10 설계 요구조건의 적용 여부에 따라 내진성능평가를 수행하였다. 그 결과, ASCE 7-10의 비정형 내진설계 요구조건을 적용한 철골 모멘트 골조의 경우 붕괴확률이 매우 낮고 상당히 보수적인 설계 결과를 나타냈으며, 본 연구에서는 비틀림 비정형 구조물의 합리적인 설계를 위해 개선된 설계 방법을 제시하였다. 제안된 방법으로 설계된 비틀림 비정형 철골 모멘트 골조의 붕괴확률은 기존의 방법으로 설계된 구조물의 붕괴확률을 보다 크지만 ASCE 7-10의 붕괴확률 요구 조건을 만족하였다.

• 미생물학회지
• /
• 제38권4호
• /
• pp.260-266
• /
• 2002

Ralstonia eutropha JMP134로부터 페놀대사의 조절에 관여하는 유전자부위를 cloning하여 염기서열을 파악하였으며 그 특성을 조사하였다. 염기서열 분석 결과 두 개의 open reading frame (ORF1 & ORF2)들을 발견하였다. ORF1은 페놀분해 구조유전자중의 마지막 인자인phlX의 stop codon으로부터 454 bp 아래에서 시작하여 총 501 개의 아미노산으로 구성되었으며 ORF2는 ORF1의 stop codon으로부터 1 bP 위쪽에서 4개의 염기쌍과 중첩된 상태에서 시작하여 총 232개의 아미노산으로 구성되어 있는 것으로 나타났다. 단백질 배열을 분석해본 결과 ORF1은 transcriptional activator로 작용하는 NtrC family에 속하는 것으로 확인되었으며, ORF2는 negative regulator로 알려진 GntR family에 속하는 것으로 나타났다. ORF1과 ORF2를 encoding하는 유전자를 각각 phlR2 와 phlA로 명명하였으며 이들의 가능한 조절기작을 고찰하였다.

• 한국통신학회논문지
• /
• 제25권1B호
• /
• pp.77-88
• /
• 2000

본 논문은 블록 단위의 비디오 코더 (Video coder)에서 최적 모드들을 적절하게 선택할 수 있는 방법을 설명한다. 비트율의 제어에 관한 문제는 버퍼가 차 있는 양에 따라 양자화 레벨이 변하는 방법이 여러 학자들로부터 연구되어 왔다. 제시된 방법은 각각의 매크로블록(macroblock)에 관한 최적 모드를 검색하지 않고 최적 결정 곡선을 검색한다는 점에서 이전의 것, 즉[4]와는 다르다. 제시된 방법은 비트율의 제어를 위하여 운동보상 또는 비 운동 보상 및 intra/inter 코딩에 관한 결정 방법까지 포함된다. 이러한 결정 방법은 주어진, 즉 고장된 결정 곡선을 이용하는 것 보다 주어진 프레임에서 설정된 비트율(bit-rate)에 따른 총체적인 왜곡을 최소화시키는 유동적인 결정 곡선을 이용하는 것이 더 바람직하다. 가열냉각 최적화기술 (annealing optimization technique)의 모의실험을 통해 곡선을 도출할 수 있다. 알고리즘은 H.261 비디오 코텍(video codec)범위에서 수행되고 모의실험 또한 그 범위에서 결과들을 보여주고 이들을 여타의 접근방법들과 비교한다.

• Journal of Periodontal and Implant Science
• /
• 제38권sup2호
• /
• pp.335-342
• /
• 2008

Purpose: Cytolethal distending toxin (CDT) considered as a key factor of localized aggressive periodontitis, endocarditis, meningitis, and osteomyelitis is composed of five open reading frames (ORFs). Among of them, the individual role of CdtA and CdtC is not clear; several reports presents that CDT is an AB2 toxin and they enters the host cell via clathrin-coated pits or through the interaction with GM3 ganglioside. So, CdtA, CdtC, or both seem to be required for the delivery of the CdtB protein into the host cell. Moreover, recombinant CDT was suggested as good vaccine material and antibody against CDT can be used for neutralization or for a detection kit. Materials and Methods: We constructed the pET28a-cdtC plasmid from Aggregatibacter actinomycetemcomitans Y4 by genomic DNA PCR and expressed in BL21 (DE3) Escherichia coli system. We obtained the antibody against the recombinant CdtC in mice system. Using the anti-CdtC antibody, we test the native CdtC detection by ELISA and Western Blotting and confirm the expression time of native CdtC protein during the growth phase of A. actinomycetemcomitans. Results: In this study we reconstructed CdtC subunit of A. actinomycetemcomitans Y4 and generated the anti CdtC antibody against recombinant CdtC subunit expressed in E. coli system. Our anti CdtC antibody can be interacting with recombinant CdtC and native CDT in ELISA and Western system. Also, CDT holotoxin existed at 24h but not at 48h meaning that CDT holotoxin was assembled at specific time during the bacterial growth. Conclusion: In conclusion, we thought that our anti CdtC antibody could be used mucosal adjuvant or detection kit development, because it could interact with native CDT holotoxin.

• 한국축산식품학회지
• /
• 제40권5호
• /
• pp.746-757
• /
• 2020

Enterotoxigenic Escherichia coli (ETEC) is the major pathogenic E. coli that causes diarrhea and edema in post-weaning piglets. In this study, we describe the morphology and characteristics of ØCJ19, a bacteriophage that infects ETEC, and performed genetic analysis. Phage ØCJ19 belongs to the family Myoviridae. One-step growth curve showed a latent phase of 5 min and burst size of approximately 20 phage particles/infected cell. Phage infectivity was stable for 2 h between 4℃ and 55℃, and the phage was stable between pH 3 and 11. Genetic analysis revealed that phage ØCJ19 has a total of 49,567 bases and 79 open reading frames (ORFs). The full genomic sequence of phage ØCJ19 showed the most similarity to an Escherichia phage, vB_EcoS_ESCO41. There were no genes encoding lysogeny, toxins, virulence factors, or antibiotic resistance in this phage, suggesting that this phage can be used safely as a biological agent to control ETEC. Comparative genomic analysis in terms of the tail fiber proteins could provide genetic insight into host recognition and the relationship with other coliphages. These results showed the possibility to improve food safety by applying phage ØCJ19 to foods of animal origin contaminated with ETEC and suggests that it could be the basis for establishing a safety management system in the animal husbandry.

• Journal of Microbiology and Biotechnology
• /
• 제22권6호
• /
• pp.742-753
• /
• 2012

Copper-containing compounds are introduced into the environment through agricultural chemicals, mining, and metal industries and cause severe detrimental effects on ecosystems. Certain microorganisms exposed to these stressors exhibit molecular mechanisms to maintain intracellular copper homeostasis and avoid toxicity. We have previously reported that the soil bacterial isolate Achromobacter sp. AO22 is multi-heavy metal tolerant and exhibits a mer operon associated with a Tn21 type transposon. The present study reports that AO22 also hosts a unique cop locus encoding copper homeostasis determinants. The putative cop genes were amplified from the strain AO22 using degenerate primers based on reported cop and pco sequences, and a constructed 10,552 base pair contig (GenBank Accession No. GU929214). BLAST analyses of the sequence revealed a unique cop locus of 10 complete open reading frames, designated copSRABGOFCDK, with unusual separation of copCD from copAB. The promoter areas exhibit two putative cop boxes, and copRS appear to be transcribed divergently from other genes. The putative protein CopA may be a copper oxidase involved in export to the periplasm, CopB is likely extracytoplasmic, CopC may be periplasmic, CopD is cytoplasmic/inner membrane, CopF is a P-type ATPase, and CopG, CopO, and CopK are likely copper chaperones. CopA, B, C, and D exhibit several potential copper ligands and CopS and CopR exhibit features of two-component regulatory systems. Sequences flanking indicate the AO22 cop locus may be present within a genomic island. Achromobacter sp. strain AO22 is thus an ideal candidate for understanding copper homeostasis mechanisms and exploiting them for copper biosensor or biosorption systems.