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Development of Recombinant Escherichia coli Expressing Rhodotorula glutinis Epoxide Hydrolase (Rhodotorula glutinis의 epoxide hydrolase 고효율 발현 유전자 재조합 Escherichia coli 생촉매 개발)

  • Lee Soo-Jung;Kim Hee-Sook
    • Journal of Life Science
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    • v.16 no.3 s.76
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    • pp.415-419
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    • 2006
  • The epoxide hydrolase (EH) of Rhodotorula glutinis which has a high enantioselectivity against aromatic epoxide substrates was expressed to high levels in Escherichia coli based on codon usage. We analysed the Preference of codon usage between the yeast, R. glutinis, and bacteria, E. coli. E. coli, Rosetta(DE3)pLysS, harbors pRARE plasmid with tRNA genes for rare-codons was employed as a host strain. The recombinant E. coli expressing R. glutinis EH showed an enhanced enantioselective hydrolysis activity toward racemic styrene oxide. Enantiopure (S)-styrene oxide with a high enantiopurity of 99% ee (enantiomeric excess) was obtained from racemic substrates.

A NOTE ON CLARKSONS INEQUALITIES

  • Cho, Chong-Man
    • Bulletin of the Korean Mathematical Society
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    • v.38 no.4
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    • pp.657-662
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    • 2001
  • It is proved that if for each n, $1\leqp_n\leq2 \;and \;the(p_n, p’_n)$ Clarkson inequality holds in each Banach space X$_{n}$ then the (t, t’) Clarkson inequality holds in ($\sum^\infty_{n=1}\; X_n)_r, \;the \ell^r-sum \;of\; X_n’s,\; where\; 1\leqr<\infty,\; t=min{p, r, r’} \;and \;p = \;inf{p_n}.$ The (p, p’) Clarkson inequality is preserved by quotient maps and a new proof of a Takahashi-Kato theorem stating that the (p, p’) Clarkson inequality holds in a Banach space X if and only if it holds in its dual space $X_*$ is given.n.

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Effects of Psychological Acceptance and Social Support on Posttraumatic Growth in Stomach Cancer Patients (위암 환자의 심리적 수용, 사회적 지지가 외상 후 성장에 미치는 영향)

  • Cho, Hyun Mi
    • Journal of Korean Clinical Nursing Research
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    • v.25 no.3
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    • pp.265-274
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    • 2019
  • Purpose: The purpose of this study was to identify the effects of psychological acceptance and social support on posttraumatic growth in stomach cancer patients. Methods: The questionnaires were administered from January 14 to February 11, 2015 to 123 subjects who had stomach cancer surgery six months prior. SPSS statistics 21.0 software was used to analyze the data for t-test, ANOVA, Pearson's correlations, Scheffé test and multiple regression analysis. Results: The results of this study are as follows: The major factors related to posttraumatic growth included gender (t=-2.72, p=.007), age (r=-.21, p=.016), having a religion (t=-3.40, p<.001), perceived importance of religion (r=.43, p<.001), seriousness of cancer diagnosis (r=.25, p=.005) and impact of cancer diagnosis (r=.32, p<.001). There were significant relationships between psychological acceptance (r=.18, p=.041) and social support (r=.32, p<.001) on posttraumatic growth. Significantly influential factors of posttraumatic growth were age (β=-.19, p=.021), perceived importance of religion (β=.41, p<.001) and family support (β=.29, p<.001), which together accounted for 36.5% of the variance in posttraumatic growth. Conclusion: The result of current study indicated that age, importance of religion, and family support influenced posttraumatic growth. Based on the findings of this study, developing nursing intervention programs focusing on increasing posttraumatic growth in stomach cancer patients is recommended.

Correlational Analysis of Supine Position Time and Sleep-related Variables in Obstructive Sleep Apnea Syndrome (폐쇄성 수면무호흡 증후군에서 앙와위 자세시간과 수면관련변인 간 상관관계 분석)

  • Kim, Si Young;Park, Doo-Heum;Yu, Jaehak;Ryu, Seung-Ho;Ha, Ji-Hyeon
    • Sleep Medicine and Psychophysiology
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    • v.24 no.1
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    • pp.32-37
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    • 2017
  • Objectives: A supine sleep position increases sleep apneas compared to non-supine positions in obstructive sleep apnea syndrome (OSAS). However, supine position time (SPT) is not highly associated with apnea-hypopnea index (AHI) in OSAS. We evaluated the correlation among sleep-related variables and SPT in OSAS. Methods: A total of 365 men with OSAS were enrolled in this study. We analyzed how SPT was correlated with demographic data, sleep structure-related variables, OSAS-related variables and heart rate variability (HRV). Multiple linear regression analysis was conducted to investigate the factors that affected SPT. Results: SPT had the most significant correlation with total sleep time (TST ; r = 0.443, p < 0.001), followed by sleep efficiency (SE ; r = 0.300, p < 0.001). Snoring time (r = 0.238, p < 0.001), time at < 90% SpO2 (r = 0.188, p < 0.001), apnea-hypopnea index (AHI ; r = 0.180, p = 0.001) and oxygen desaturation index (ODI ; r = 0.149, p = 0.004) were significantly correlated with SPT. Multiple regression analysis revealed that TST (t = 7.781, p < 0.001), snoring time (t = 3.794, p < 0.001), AHI (t = 3.768, p < 0.001) and NN50 count (t = 1.993, p = 0.047) were associated with SPT. Conclusion: SPT was more highly associated with sleep structure-related parameters than OSAS-related variables. SPT was correlated with TST, SE, AHI, snoring time and NN50 count. This suggests that SPT is likely to be determined by sleep structure, HRV and the severity of OSAS.

Effects of a Porcine MC4R Polymorphism(892G>A) on Carcass Traits in Commercial Pigs (돼지 MC4R 유전자 892G>A 다형성이 비육돈의 도체형질에 미치는 영향)

  • Han, S.H.;Lee, S.S.;Ko, M.S.;Seong, P.N.;Park, B.Y.;Cho, I.C.
    • Journal of Animal Science and Technology
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    • v.49 no.5
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    • pp.569-576
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    • 2007
  • receptor(MC4R) gene and carcass traits was examined in randomly selected commercial pigs. A porcine MC4R gene was genotyped for Asp298Asn(nt. 892G>A) by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). A total of three genotypes, A/A, A/G, and G/G, were found with 28.8, 22.8, and 48.4% frequencies, respectively. In the whole population, pigs containing 892A/- showed significantly higher marbling score than those of homozygotes G/G(P<0.05). Two homozygotes, A/A and G/G showed lower in meat color score but higher in water holding capacity than those of heterozygotes A/G(P<0.01). However, the carcass weight of the barrows containing wild type -/G was significantly higher(i.e. more than 2.5kg) than those of homozygotes A/A(P<0.05). The effects of each genotype on carcass traits in the gilts were similar to those of the whole population, but not in barrows, suggesting an unknown sex-related effect on carcass traits. This study suggested that the genotype MC4R A/- could improve the meat quality in the commercial pig production. However, since the genetic polymorphism of MC4R gene differentially affected the carcass traits in sex-related manner, therefore, both parameters, the sex and genotype, should be considered for marker-assisted selection in commercial pig production.

Serum and Urinary Levels of Soluble Interleukin-2 receptor in Childhood Minimal Change Nephrotic Syndrome and Focal Segmental Glomerulosclerosis (소아의 미세변화형 신증후군 및 초점성 분절성 사구체 경화증 환아에서 혈청 및 요의 용해성 인터루킨-2수용체)

  • Ha, Il-Soo;Cheong, Hae-Il;Choi, Yong
    • Childhood Kidney Diseases
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    • v.3 no.1
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    • pp.27-34
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    • 1999
  • Purpose: This study was designed to investigate the changes in soluble interleukin-2 receptor (sIL-2R) level in sera and urines of children with primary nephrotic syndrome, eliminating the confounding effects of age, proteinuria, and steroid treatment. Methods: Soluble IL-2R was measured by ELISA in sera and urines from patients with minimal change nephrotic syndrome or focal segmental glomerulosclerosis as well as from healthy controls. The serum levels and urinary sIL-2R/creatinine ratios were compared between control group and the 12 patient groups divided by their ages (0-1, 2-4, over 5 years), and presence or absence of proteinuria and/or steroid treatment (PU+Tx-, PU+Tx+, PU-Tx+, PU-Tx-). Results: Though the differences were not statistically significant probably because of the small numbers, serum sIL-2R levels seemed to be higher in younger age groups both in patients and control group. Nephrotic children did not show higher serum levels than normal children. Among the patients, proteinuric condition seemed to raise and steroid treatment tended to suppress the serum sIL-2R levels. Urinary sIL-2R/creatinine ratios were higher in younger age groups, more significantly in patients (P<0.001). Proteinuria and steroid treatment affected the urinary sIL-2R/creatinine ratios by the same way as the serum sIL-2R levels. Serum sIL-2R levels and urinary sIL-2R/creatinine ratios were not different between groups of different histologic findings or steroid responsiveness (P>0.05). Conclusion: Serum sIL-2R levels and the urinary sIL-2R/creatinine ratios were higher in younger age, and they were not higher in nephrotic patients compared to control group. The patients in relapse showed higher levels, while the levels were suppressed with steroid treatment. In proteinuric state, urinary sIL-2R/creatinine ratios reflected serum sIL-2R levels.

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Through SNS Eat Out Select Properties, Customer Satisfaction, Recommendation of Others Affect Relations (SNS를 통한 외식선택속성이, 고객만족도, 타인추천 영향관계)

  • Kim, Geon-Whee;Han, Ji-Soo
    • Culinary science and hospitality research
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    • v.22 no.4
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    • pp.143-155
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    • 2016
  • This study conducted a survey and emperical analysis of SNS based consumer experience visits to eat out companies. A total of 350 questionnaires were collected of which 28 were deemed unreliable, leaving 322 responses to be used in the analysis. SPSS 22.0 was employed to carry out frequency analysis, factor analysis, and reliability analysis, correlation analysis, and regression analysis. First, choose eat out properties multiple regression analysis on the impact on customer satisfaction, overall explanatory power of the regression equation $R^2=.521$ (adjusted $R^2=.515$) and F values were analyzed statistically significant the influence of the positive (+) with 86.325 p=0.000 and the regression equation. Content type pursuit of independent variables (B=0.540, p<0.001), seeking safety type (B=0.292, p<0.001) were significantly appeared showed the influence of the positive (+), Pursuit convenience-type (B=0.071, p<0.001) and the pursuit of dignity type (B=0.002, p<0.001) the sub-showed the influence of (-). Second, the multiple regression analysis on the impact on customer satisfaction to others is recommended explanatory power is $R^2=.539$ (adjusted $R^2=.538$) and F values are analyzed by a statistical regression equation p=0.000 and 374.765 in significance showed that the model fit the regression line. Customer satisfaction is the independent variable was the influence of the positive (+) to (B=0.540, p<0.001).

The Relationship between Health Belief.Self-efficacy and Exercise.Diet Compliance in Coronary Heart Disease Patients (관상동맥질환자의 건강신념 및 자기효능감과 운동 및 식이요법 이행과의 관계)

  • Nam, Myung-Hee;Kim, Chung-Nam;Oh, Yun-Jung
    • Research in Community and Public Health Nursing
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    • v.8 no.2
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    • pp.262-276
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    • 1997
  • This study evaluates the relationship between health belief. self-efficacy and exercise and diet compliance in coronary heart disease patients. The study subjects were 96 CHD patients who visited the outpatient clinic at 3 general hospitals in Taegu city from April 3, 1997 to May 3, 1997. Data was collected by the researcher and two registered nurses who work at a cardiac outpatient clinic. Face-to-face interview was conducted. Moon's health belief scale(l990) and Hicky and others' Cardiac Diet Self-Efficacy Instrument (CDSEI, 1992), Cardiac Exercise Self-Efficacy Instrument (CESEI, 1992) were used. The instrument developed by the researcher based on the reference review was used to measure exercise and diet compliance. The data were analyzed by using descriptive statistics, Pearson correlation coefficient, t-test, ANOVA, Tukey verification and Stepwise multiple regression with the SAS program. The results of this study were as follows; 1. The degree of health belief(score range: 1-4) perceived benefit: 3.06 barrier: 2.04 severity: 2.93 2. The degree of self-efficacy(score range: 1-5) exercise self-efficacy: 2.91 diet self-efficacy: 3.32 3. The degree of compliance (score range: 1-4) exercise compliance: 2.34 diet compliance: 2.95 4. The exercise compliance had a positive correlation with perceived benefit(r=0.5327, p=0. 0001), severity(r=0.2780, p=0.0061), exercise self-efficacy(r=0.6675, p=0.0001), and a negative correlation with barrier{r= -0.4236, p=0.0001). The diet compliance had a positive correlation with perceived benefit (r=0.6439, p=0.0001), severity(r=0.4244, p=0.0001), diet self-efficacy(r=0.6629, p=0.0001), and a negative correlation with barrier{r= -0.5098, p=0.0001). 5. According to pt's education level, (F=3.02, p=0.0336), received massage from mass media on exercise and diet(t=3.81, p=0.0002), presence of cardiac patients in the family members or friends(t=2.00, p=0.0478), created significant differences in exercise compliance. According to occuption(F=3.03, p=0.0215), hospitalized experience(t=4.59, p=0.0000), presence of chest pain(t=3.63, p=0.0005), there was also a significant difference in diet compliance. 6. The combination of exercise self-efficacy, perceived benefit and pt's education level explained 50.18% of the variance in exercise compliance. The combination of diet self-efficacy, perceived benefit and barrier explained 56.76% of the variance in diet compliance. On the basis of the above findings, the follow ing recommendations are suggested: 1. To promote the exercise. diet compliance for CHD patients, a well organized health teaching and nursing intervention program should be developed. 2. More research is needed to investigate other variables affecting exercise and diet compliance of CHD patients. 3. To promote self-efficacy and a positive health belief in CHD patients, a well organized and an approachable nursing intervention program should be developed. 4. Factors other than diet. exercise compliance should be evaluated to discover the impact on CHD patients.

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Effect of Sociocultural Attitudes toward Appearance, Social Pressure and Stress on University Students' Body Figure Discrepancy (대학생의 외모 관련 사회문화적 태도 및 사회적 압박감, 스트레스가 신체상 불일치에 미치는 영향)

  • Lee, Eunmi;Kim, Yujeong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.12
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    • pp.173-181
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    • 2017
  • This study was conducted to investigate the effects of sociocultural attitude toward appearance, social pressure, and stress on university students' body figure discrepancy. A total of 363 students were recruited from a university in C and A city. Data were collected by self-reported questionnaires from Oct 27 to Dec 9, 2016. Statistical analyses of the collected data were conducted using SPSS 20.0 and consisted of descriptive statistics, Pearson's correlation, and multiple regression. A positive relationship was observed between body figure discrepancy and female's sociocultural attitude toward appearance (r=.406, p=.006), peer pressure (r=.197, p<.001), parental pressure (r=.176, p=.002), and stress (r=.218, p<.001). Body figure discrepancies increased with peer pressure (${\beta}=.159$, p=.007), stress (${\beta}=.186$, p=.002), and BMI (${\beta}=.186$, p=.002), and these factors accounted for 10.9% of the body figure discrepancy. To reduce body figure discrepancy, it is necessary to develop health education and promotion programs that enable university students to manage weight and stress for health maintenance and to have positive self-assessment without being hurt by other people's view or evaluation.

ON SOME NEW CLASSES OF COMPACT-LIKE BITOPOLOGICAL SPACES

  • Afsan, BM Uzzal
    • Journal of the Chungcheong Mathematical Society
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    • v.33 no.2
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    • pp.271-285
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    • 2020
  • In this paper, we have introduced a new type of covering property ${\beta}^t_{({\omega}_r,s)}$-closedness, stronger than $P^t_{({\omega}_r,s)}$-closedness [3] in terms of (r, s)-β-open sets [9] and β-ωt-closures in bitopological spaces along with its several characterizations via filter bases and grills [15] and various properties. Further grill generalizations of ${\beta}^t_{({\omega}_r,s)}$-closedness (namely, ${\beta}^t_{({\omega}_r,s)}$-closedness modulo grill) and associated concepts have also been investigated.