• Title/Summary/Keyword: P-S-R

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Comparative Molecular Analysis of Freshwater Centric Diatoms with Particular Emphasis on the Nuclear Ribosomal DNA of Stephanodiscus (Bacillariophyceae)

  • Ki, Jang-Seu
    • ALGAE
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    • v.24 no.3
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    • pp.129-138
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    • 2009
  • DNA-based discrimination of species is a powerful way for morphologically otherwise similar species, like centric diatoms. Here, the author sequenced long-range nuclear ribosomal DNAs, spanning from the 18S to the D5 region of the 28S rDNA, of Stephanodiscus, particularly including a Korean isolate. By comparisons, high DNA similarities were detected from the rDNAs of nine Stephanodiscus (>99.4% in 18S rDNA, >98.0% in 28S rDNA). Their genetic distances, however, were significantly different (Kruskal-Wallis test, p < 0.01) compared to two related genera, namely Cyclotella and Discostella. In addition, genetic distances of 18S rDNAs were significantly different (Student’s t-test, p = 0.000) against those of the 28S rDNAs according to individual genera (Cyclotella, Discostella, and Stephanodiscus). Phylogenetic analyses showed that Stephanodiscus and Discostella showed a sister taxon relationship, and their clade was separated from a cluster of Cyclotella (1.00 PP, 100% BP). This suggests that Stephanodiscus has highly conserved sequences of both 18S and 28S rDNA; however, Stephanodiscus is well-separated from other freshwater centric diatoms, such as Cyclotella and Discostella, at the generic level.

Evaluation of the inhibitive characteristics of 1,4-dihydropyridine derivatives for the corrosion of mild steel in 1M $H_2SO_4$

  • Sounthari, P.;Kiruthika, A.;Sai santhoshi, J.;Chitra, S.;Parameswari, K.;Selvaraj, A.
    • Corrosion Science and Technology
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    • v.12 no.2
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    • pp.65-78
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    • 2013
  • The present investigation deals with the corrosion inhibition of mild steel in 1M $H_2SO_4$ with 1, 4-dihydro pyridine and its derivatives prepared using microwave activation method. The synthesis of inhibitor was confirmed by IR spectra. The effect of 1, 4-dihydropyridine derivatives on the corrosion inhibition of mild steel in 1M $H_2SO_4$ was studied using weight loss and electrochemical polarization techniques. Influence of temperature (303-333K) and synergistic effect of halide ions ($I^-$, $Br^-$ and $Cl^-$) on the inhibition behaviour was also studied. Corrosion products on the metal surface were analyzed by scanning electron microscopy (SEM) and a possible mechanism of inhibition by the compounds is suggested. Thermodynamic parameters were calculated using weight loss data in order to elaborate the mechanism of corrosion inhibition. Polarization measurements revealed that the studied compounds acted as mixed type inhibitor but slightly anodic in nature. Electrochemical impedance measurements revealed that the compounds were adsorbed onto the carbon steel surface and the adsorption obeyed the Langmuir adsorption isotherm. The synergistic effect of halide ions on the IE increases with increase in concentration. The IE obtained from atomic absorption spectrophotometric studies was found to be in good agreement with that obtained from the conventional weight loss method. SEM revealed the information of a smooth, dense protective layer in presence of the inhibitors.

Susceptibility to Calonectria ilicicola in Soybean Grown in Greenhouse and Field

  • Kim, K. D.;Russin, J. S.;Snow, J. P.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.43 no.4
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    • pp.239-244
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    • 1998
  • Susceptibility of soybean cultivars to Calonectria illicicola was evaluated in a greenhouse by inoculating seedlings with mycelium in agar discs placed on the stems at the soil line. A range of responses was detected among cultivars following inoculation with a virulent isolate of C.ilicicola. Rankings of cultivars between greenhouse tests 1 and 2 were similar for disease severity and areas under the disease progress curves (AUDPC). In addition, rankings of cultivars for Final disease severity were highly correlated with AUDPC in test 1 ($r_s$ =0.88, t =5.48, p<0.001), test 2 ($r_s$ =0.99, t =22.10, p<0.001), and when tests were combined ($r_s$=0.89, t=5.82, p<0. 001). Final disease severity and AUDPC consistently identified Asgrow 7986, Braxton, Cajun, and Forrest as soybean cultivars least susceptible to red crown rot. In 1993 and 1994 field tests, a range in disease susceptibility was observed for tested cultivars but none was completely resistant. Soybean cultivars Braxton, Cajun, and Forrest, which were least susceptible to red crown rot in greenhouse tests, also ranked among cultivars with the lowest disease incidence and AUDPC in field tests. Comparisons .between rankings of the eight cultivars common to greenhouse and field tests showed a correlation between final disease severity from combined greenhouse tests and both final disease incidence ($r_s$=0.63, t =1.99, p<0.1) and AUDPC ($r_s$=0.60, t =1.82, p < 0.2) from the combined field tests. However, AUDPC from greenhouse tests did not correlate with either final disease incidence or AUDPC from field tests. The green-house screening method provided consistent results between greenhouse and field tests and successfully identified the least susceptible cultivars Braxton, Cajun, and Forrest.

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Relationship between BCS during Prepartum, Calving and Postpartum Periods and Fertility of Korean Brown Cattle (한우에서 분만 전, 분만 시 및 분만 후의 body condition score와 이후의 번식능력과의 상관관계)

  • Choi, In-Su;Kim, Ui-Hyung;Kang, Hyun-Gu;Kim, Ill-Hwa
    • Journal of Veterinary Clinics
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    • v.25 no.4
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    • pp.280-285
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    • 2008
  • This study evaluated the correlation between the body condition score (BCS) during prepartum, calving and postpartum periods and the reproductive performance of Korean brown cattle. The BCSs of 33 cows who underwent 73 calvings over a two and a half period [the parities of the cows ranged from 1 to 4 ($mean{\pm}SD,\;2.0{\pm}0.9$)] were scored at months 2 and 1 prepartum, calving, and every month postpartum until month 7. A marked prepartum loss of BCS in the month preceding calving was noted. The correlations between the interval from calving to conception and the month 1 prepartum, calving and months 1 and 2 postpartum BCSs were analyzed by Pearson correlation analysis. The correlation between the interval from calving to conception and the prepartum body condition loss was also evaluated. The interval from calving to conception correlated positively with the month 1 prepartum BCS (r = 0.389, P = 0.0007) and the prepartum body condition loss (r = 0.488, P < 0.0001) but did not correlate significantly with the BCS at calving (r=-0.070, P=0.56) or months 1 (r=0.107, P=0.37) or 2 (r=0.102, P=0.39) postpartum. The prepartum body condition loss correlated positively with the month 1 prepartum BCS (r=0.587, P<0.0001). In conclusion, the month 1 prepartum BCS may be a good criterion for predicting subsequent reproductive performance. Moreover, the prevention of obesity and/or excessive prepartum body condition loss may result in higher fertility in Korean brown cattle.

ON SOME MEASURE RELATED WITH POISSON INTEGRAL ON THE UNIT BALL

  • Yang, Gye Tak;Choi, Ki Seong
    • Journal of the Chungcheong Mathematical Society
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    • v.22 no.1
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    • pp.89-99
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    • 2009
  • Let $\mu$ be a finite positive Borel measure on the unit ball $B{\subset}\mathbb{C}^n$ and $\nu$ be the Euclidean volume measure such that ${\nu}(B)=1$. For the unit sphere $S=\{z:{\mid}z{\mid}=1\}$, $\sigma$ is the rotation-invariant measure on S such that ${\sigma}(S)=1$. Let $\mathcal{P}[f]$ be the invariant Poisson integral of f. We will show that there is a constant M > 0 such that $\int_B{\mid}{\mathcal{P}}[f](z){\mid}^{p}d{\mu}(z){\leq}M\;{\int}_B{\mid}{\mathcal{P}}[f](z)^pd{\nu}(z)$ for all $f{\in}L^p({\sigma})$ if and only if ${\parallel}{\mu}{\parallel_r}\;=\;sup_{z{\in}B}\;\frac{\mu(E(z,r))}{\nu(E(z,r))}\;<\;\infty$.

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Peptoniphilus mikwangii-specific quantitative real-time polymerase chain reaction primers

  • Park, Soon-Nang;Kook, Joong-Ki
    • International Journal of Oral Biology
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    • v.44 no.3
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    • pp.96-100
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    • 2019
  • The purpose of this study was to develop Peptoniphilus mikwangii-specific quantitative real-time polymerase chain reaction (qPCR) primers based on the 16S ribosomal RNA (16S rDNA) gene. The specificity of the primers was determined by conventional PCR using 29 strains of 27 oral bacterial species including P. mikwangii. The sensitivity of the primers was determined by qPCR using the purified genomic DNA of P. mikwangii KCOM $1628^T$ (40 ng to 4 fg). The data showed that the qPCR primers (RTB134-F4/RTB134-R4) could detect P. mikwangii strains exclusively and as little as 40 fg of the genomic DNA of P. mikwangii KCOM $1628^T$. These results suggest that the developed qPCR primer pair can be useful for detecting P. mikwangii in epidemiological studies of oral bacterial infectious diseases.

The Relationship between Communication Self-confidence, Critical Thinking Disposition and Nursing Professionalism in Nursing Students (간호대학생의 의사소통 자신감, 비판적사고 성향, 간호 전문직관의 관계)

  • Woo, Ju-Hyun;Song, Jung-Hee
    • Journal of Industrial Convergence
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    • v.18 no.4
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    • pp.101-110
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    • 2020
  • The purpose of this study was to find the qualities of nursing students who need to improve their communication self-confidence by identifying their communication self-confidence, critical thinking disposition, and nursing professionalism. The study's data was collected from August 2019 to September 2019. Informed consent was received from 122 students who were in their senior of nursing college. The collected data was analyzed using SPSS WIN 20.0 program. As a result of the study, the score for communication self-confidence was 32.31, the score for a critical thinking disposition was 101.34, and the score for nursing professionalism was 3.78. Communication confidence was found to have a significant positive correlation with critical thinking tendency(r=.568, p<.001) and nursing professional intuition(r=.282, p<.001). The critical thinking tendency was found to have a positive correlation with communication confidence(r=.568, p<.001) and nursing professional intuition(r=.361, p<.001). Nursing professional intuition was also found to have a positive correlation with communication confidence(r=.282, p<.05) and critical thinking propensity(r=.361, p<.001).

A Converged Study on the Influence on the Suicide of Idea the Elderly Living Alone. (독거노인의 자살생각 영향 요인에 대한 융합연구)

  • Kim, Jeong-Suk
    • Journal of Convergence for Information Technology
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    • v.8 no.5
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    • pp.11-17
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    • 2018
  • This study was conducted to analyze the factor of suicide ideation in the elderly living alone. This study is a descriptive research study of 175 elderly living alone in K&C region. It is a frequency analysis, correlation analysis, simple rare analysis. AMOS statistics were performed. Data collection was from January 2017 to March 2017. The results of this study are as follows. social activity (r=-.106, p<.05), subjective health status (r=-.292, p<.01) Self-esteem (r=-.069, p<.05), mind control(r=-.201, p<.01), and depression(r=.023, p<.01), stress (r=.320, p<.05). Suicidal influence factor 43.5% explanatory power. In order to prevent the suicide of the elderly living alone, It will be necessary to seek active nursing intervention to help prevent suicide.

The Influence of Stress and Emotion Regulation Ability of Health College Students on University Life Adjustment (보건계열 대학생들의 스트레스와 정서조절에 따른 대학생활적응 관련성 연구)

  • Kang, Hyun-Joo
    • Journal of the Korea Convergence Society
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    • v.12 no.9
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    • pp.65-71
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    • 2021
  • The purpose of this study is to investigate the factors that affect stress, emotional regulation, and adjustment to college life of health college students. The survey was conducted from June 21 to 30, 2021 for 190 health students in G and J area. The data were analyzed by frequency analysis, reliability analysis, simple correlation analysis, and multiple regression analysis. The results showed that positive perception had positive correlation with negative perception(r=-.286,p<.01), emotional regulation (r=.296,p<.01), and university life adjustment(r=.520,p<.01). Negative perception was correlated with emotional regulation(r=-.619,p<.01), university life adjustment (r=-.428,p<.01) and negative(-). Emotional regulation had a positive correlation with university life adjustment(r=.341,p<.01). Positive perception, negative perception, and emotional regulation, which are sub-factors of perceived stress, were the factors affecting the adjustment to university life, and the explanatory power was 84%. Therefore, it is necessary to develop a program that can improve the adjustment of college students in health.

MiR-21 Upregulation Induced by Promoter Zone Histone Acetylation is Associated with Chemoresistance to Gemcitabine and Enhanced Malignancy of Pancreatic Cancer Cells

  • Song, Wei-Feng;Wang, Lei;Huang, Wei-Yi;Cai, Xun;Cui, Jiu-Jie;Wang, Li-Wei
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.12
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    • pp.7529-7536
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    • 2013
  • Background and Aims: MicroRNA-21 (miR-21) is reported to be overexpressed and to contribute to proliferation, apoptosis and gemcitabine resistance in pancreatic ductal adenocarcinomas (PDACs). The aims of this study were to explore regulation of miR-21 expression by epigenetic change and its impact on chemoresistance and malignant properties of of pancreatic cancer. Materials and methods: We retrospectively collected 41 cases of advanced pancreatic cancer patients who were sensitive or resistant to gemcitabine and assessed levels of serum circulating miR-21 for correlation with cytotoxic activity. Histone acetylation in the miR-21 promoter was also studied in gemcitabine-sensitive and gemcitabine-resistant PDAC cells. Gemcitabine-resistant HPAC and PANC-1 cells were transfected with pre-miR-21 precursors (pre-miR-21) and antisense oligonucleotides (anti-miR-21), and were treated with TSA. Finally, invasion and metastasis assays were performed and alteration in mir-21, PTEN, AKT and pAKT level was evaluated in these cells. Results: Serum miR-21 levels were increased in gemcitabine-resistant PDAC patients compared with gemcitabine-sensitive subjects. The miR-21 levels were increased in 6 PDAC cells treated with gemcitabine significantly, associated with 50% inhibitory concentrations ($IC_{50}s$). Histone acetylation levels at miR-21 promoter were increased in PDAC cells after treatment with gemcitabine. Enhanced invasion and metastasis, increased miR-21 expression, decreased PTEN, elevated pAKT level were demonstrated in gemcitabine-resistant HPAC and PANC-1 cells. Pre-miR-21 transfection or TSA treatment further increased invasion and metastasis ability, decreased PTEN, and elevated pAKT levels in these two lines. In contrast, anti-miR-21 transfection could reverse invasion and metastasis, and PTEN and pAKT expressions induced by gemcitabine. Conclusions: MiR-21 upregulation induced by histone acetylation in the promoter zone is associated with chemoresistance to gemcitabine and enhanced malignant potential in pancreatic cancer cells.