• YAKHAK HOEJI
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• v.40 no.2
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• pp.225-229
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• 1996

The cytotoixic effect of Pylorae Herba (Pyrola japonica Klenze) against L1210 and K-562 cells was studied in vitro. The methanolic extract of Pylorae Radix was add ed to the culture of L1210 cells and K-562 cells for the cytotoxic activity and the ED50 values of hexane, ethylacetate, buthanol and water fractions from methanolic extract were determined using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide) assay. The active constituent isolated by bioassay guided fractionation followed by purification gave rise to a yellow needle crystal and was clarified to be chimaphilline by the comparison with the published data. The average life spans with it were not prolonged significantly on tumor growth in hybrid female mouse (BDF1-KIST) inoculated subcutaneously with P388 cells.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.3
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• pp.914-918
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• 2004

Cytotoxic and antifungal activities were strongly shown by Fr. 4 (218 mg, P388 IC/sub 50/ 652 ng/ml at 75 μg/disc, IC50 834 ng/ml at 30 μg/disc and HM 8). The subfraction 4 - 3 that showed the most activity was cytotoxic to murine leukaemia cells ATCC CCl 46 P388D1, (44.0 mg, P388 IC50 302 ng/ml at 30 μg/disc).

• Korean Journal of Pharmacognosy
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• v.23 no.3
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• pp.132-136
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• 1992

The whole plants of Selaginella tamariscina, Orostachycis japonicus, the cortex of Ulmus mandshurica, and the wood of Alnus japonica have been used as folk medicine for treating cancer. The cytotoxic activity of these plants were tested using a calorimetric tetrazolium assay (MTT assay). S. tamariscina and A. japonica showed mild $IC_{50}$ value, comparing with O. japonicus and U. mandshurica. So, MeOH extracts of S. tamariscina and A. japonica were partitioned into $CHCl_3$, EtOAc and n-BuOH, successively. The $CHCl_3$, EtOAc and BuOH fractions of S. tamariscina and A. japonica showed low percent of survival against $P_{388}$ and $MKN_{45}$ cells respectively. To isolate active components, they were subjected to silica gel column chromatography. Compound I was obtained from EtOAc extracts of S. tamariscina and identified as amentoflavone by chemical and spectral analysis. Amentoflavone inhibited the survival of P388 cells dose dependently, while not clearly inhibited that of $MKN_{45}$ cells.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.6
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• pp.1656-1658
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• 2005

The effects of chloroform extract from Lepidolaena tayiorii (L. tayiorii) on antifungal activity were investigated. The crude chloroform extract of L. tayiorii inhibited the growth of the Gram positive bacteria Bacillus subtilis ATCC 19659, (5 mm inhibition zone at $150{\mu}g/disc$) and the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185, (6 mm inhibition zone at $150{\mu}g/disc$), and cytotoxic to P388 murine leukaemia cells ATCC CCL 46 P388D1, ($IC_{50}\;405.0{\mu}g/mL\;at\;150{\mu}g$/disc) and cytotoxic to BSC monkey kidney cells (@ 5 mg/mL, $150{\mu}g/disc;$ +++: 100% activity). We suppose that this crude chloroform extract of L. tayiorii is the strong antimicrobial and cytotoxic activities.

• Korean Journal of Pharmacognosy
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• v.31 no.1
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• pp.51-56
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• 2000

This study was carried out to evaluate cytotoxic effects of the roots of Sophora flavescens Ait. extracts on murine leukemia tumor cells lines $(P388D_1\;and\;L1210)$. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The comparison of $IC_{50}$ values of the ethyl acetate of Sophora flavescens Ait. extract in leukemia cell lines showed that their susceptibility to these extracts decreased in the following order : Adriamycin>Fr.4>Fr.5>Fr.3>Fr.1>Fr.2 by the MTT assay. These results suggest that the fraction 4 of the ethyl acetate soluble extract of Sophora flavescens Ait. may be a valuable choice for the studies on the treatment of murine leukemia cell lines.

• KSBB Journal
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• v.5 no.2
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• pp.113-118
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• 1990

This experiment was carried out to investigate the immuno-regulatory effects of histamine on IL-1 synthesis and Ca2+ uptake in P388Dl macrophage-like cell line. The addition of histamine (10-8-10-3 M) increased IL-1 production in P388D1, cells, in a dose dependent manner, the treatment of EGTA (10-7-10-4M) and Co2+ ion (10-5-10-4M) decreased macrophage-derived IL-1 activity, and the pretreatment of histamine at the peak of 10-4M significantly enhanced Ca2+ uptake to P388Dl Cells. These results suggested that exogenous histamine was effective on IL-1 production from macrophage and the intracellular Ca2+ uptake play a important role in histamine-stimulated IL-1 synthesis.

• Journal of Pharmaceutical Investigation
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• v.24 no.4
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• pp.245-250
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• 1994

An attempt was made to develop new water-soluble antitumor Pt(ll) complexes containing ethylenediamine, and their structures were determined by infrared spectroscopy, $^{13}C$ nuclear magnetic resonance and elemental analysis. Their antitumor activities in vitro against L-1210, p-388 leukemia cells and M-14 melanoma cells were investigated and acceptable antitumor activity was found as compared with cisplatin.

• Natural Product Sciences
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• v.24 no.4
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• pp.284-287
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• 2018

A new isoprenylated acetophenone, acronyculatin P (1) as well as two known compounds, 3',5'-diisoprenyl-2',4'-dihydroxy-6'-methoxyphenylethanone (2) and 3'-isoprenyl-2',4',6'-trihydroxyphenylethanone (3) were isolated from the stem bark of Acronychia pedunculata (L.) Miq. The structures were determined by HRESIMS, 1D and 2D NMR. The inhibitory activity of the isoprenylated acetophenone derivatives against murine leukemia P-388 cells showed compound 1 moderate activity with $IC_{50}$ $15.42{\mu}M$.

• Archives of Pharmacal Research
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• v.29 no.2
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• pp.123-130
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• 2006

1,4-Naphthoquinones are widely distributed in nature and many clinically important antitumor drugs containing a quinone moiety, such as anthracyclines, mitoxantrones and saintopin, show excellent anticancer activity. In this study, 2- or 6-substituted 5,8-dimethoxy-1,4-naphthoquinone (DMNQ) and 5,8-dihydroxy-1,4-naphthoquinone (DHNQ) derivatives were synthesized, and their cytotoxic activity against L1210 and P388 cancer cells was examined. Their antitumor activity was also assessed in mice bearing S-180 cells in the peritoneal cavity. In comparison with the DMNQ derivatives, the DHNQ derivatives exhibited more potent bioactivities than the DMNQ derivatives against both L1210 and P388 cells in vitro and S-180 cells in vivo. The $ED_{50}$ values of the DHNQ derivatives against P388 cells were in the range of 0.18-1.81 ${\mu}g/mL$ whereas those of the DMNQ derivatives were in the range of 0.26-40.41 ${\mu}g/mL$. The T/C ($\%$) values of the DHNQ derivatives, 8, 17, 18, 19, and 20, were found to be comparable to or even better than that of adriamycin. It was also observed that the 2-substituted derivatives (8, 19, 20) showed better antitumor activity than the 6-substituted derivatives (7, 17, 18) in the mice bearing S-180 cells in the peritoneal cavity.

• Journal of Ginseng Research
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• v.13 no.2
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• pp.242-247
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• 1989

The effect of ginseng extract and sodium ascorbate (vitamin C) administered separately or in combination on the some cancer cells cultured in vitro have been examined. Mouse leukemic cells (L1210 and P388), human rectal cancer cells (HRT-18) and human colon cancer cells (HCT-48) were used for the experiment. When given separately, the growth rate for each kind of cancer cell was inhibited In proportion to the concentration of ginseng extract or vitamin C. The inhibitory effect on the growth rate of the cancer cells was stronger in ginseng extract than in vitamin C except for the HCT-48 cells. Based on the cytotoxic activity, combined administration of ginseng extract and vitamin C demonstrated a synergistic inhibition of cancer cell growth. The cytotoxic activities of ginseng extract and vitamin C on the mouse leukemic cells were more sensitive than on human colon cancer cells. And the sensitivity of cytotoxic activity was somewhat different in different cancer cell lines.