• 대한치과교정학회지
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• 제20권1호
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• pp.135-156
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• 1990

This study was undertaken to investigate the difference of chin morphology and mandibular form in relation to different mandibular growth direction. The subject was divided into three group i.e., control group, vertical group, and horizontal group, according to the criteria of $Bj{\ddot{o}}rk$ sum, and each group was composed of 15 females and 15 males. Medial axis analysis in addition to the routine cephalometric analysis using P.I.A.S. (personal image analysing system) was carried out to find out the differences of mandibular morphology on each group. The results were as follows: 1. The area of symphysis was larger in horizontal growth group than that of vertical growth group. 2. Protruding chin area was also larger in horizontal growth group than that of vertical growth group. 3. There was a close correlationship between protruding chin area and other form of mandible. 4. Antegonial notch depth and ramus posterior contour depth was deeper in vertical growth group than in horizontal growth group, and antegonial notch depth was more influenced by anterior part of notch than posterior part of notch. 5. Mental medial axis and incisal medial axis length, in relation to corpus medial axis length was larger in vertical growth group than in horizontal growth group.

• 치과방사선
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• 제21권1호
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• pp.91-98
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• 1991

This study was made to analyze the positional relationship of mandible to hinge axis in normal subject as the diagnostic criteria of T.M.J. dysfunction. The author has conducted the study using the Cephalometric P-A view, and dental of 53 males and 51 females. By measurement, the standard value of cranio-facial and occlusal asymmetry in normal subject obtained. The following results were obtained: 1. Although the degree of the difference between right and left width in each measured line was small, asymmetry in normal subject could be identified. 2. In male, measurement between genial tubercle and hinge axis is 127.20±6.06㎜ in left, 125.83±6.25㎜ in right, and angle between genial tubercle and hinge axis is 48.48±3.53° in left, 49.58±3.72° in right. In female, measurement is 119.32±5.81㎜ in left, 118.82±5.35㎜ in right and angle is 48.06±2.15° in left, 48.17±2.31° in left, 48.17±2.31° in right. 3. In male, difference between left and right canine cusp tip of maxilla about X-bar is 0.70±0.59㎜, and that of central pit of first molar of maxilla is 0.98±0.75㎜. In female, difference is 0.64±0.52㎜ in canine and 0.92±0.76㎜ in molar.

• 대한족부족관절학회지
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• 제10권2호
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• pp.140-143
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• 2006

Purpose: To present tibial axis-talar ratio and tibia-ankle surface angle in lateral measured on standing ankle lateral radiographs of adults who did not have specific ankle pathology. Materials and Methods: On Fifty-six radiographs without ankle osteoarthritis and malalignment, radiographic measures were performed with use of a custom dizitizing program based on $PV-WAVE^{(R)}$. AP ankle alignment was quantified by tibia axis-talar ratio (TTR) and tibia ankle surface angle in lateral (TLS angle). The data was compared with previously reported american data and analyzed using a t-test. Results: The average TTR and TLS angle of our series were $33.9{\pm}3.3%$, and no significant difference compared with reported american adults (p=0.152). Conclusion: The T-T ratio and TLS angle measured on standing ankle lateral radiographs of our series were $33.9{\pm}3.3%$ and $80.4{\pm}3.3^{\circ}$. This measures appear to be a useful measure for determining AP ankle alignment.

• Computers and Concrete
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• 제26권1호
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• pp.95-106
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• 2020

Castellated beams fabricated from standard I-sections are being used for several structural applications such as commercial and industrial buildings, multistory buildings, warehouses and portal frames in view of numerous advantages. The advantages include enhanced moment of inertia, stiffness, flexural resistance, reduction in weight of structure, by passing the used plate girders, the passage of service through the web openings etc. In the present study, experimental and numerical investigations were carried out on concrete encased steel castellated beams with hexagonal openings under flexural loading. Various positions of openings such as along the neutral axis, above the neutral axis and below the neutral axis were considered for the study. From the experimental findings, it has been observed that the load-carrying capacity of the castellated beam with web opening above neutral axis is found to be higher compared to other configurations. Nonlinear finite element analysis was performed by using general purpose finite element software ABAQUS considering the material nonlinearities. Concrete damage plasticity model was employed to model the nonlinearity of concrete and elasto-plastic model for steel. It has been observed that FE model could able to capture the behaviour of concrete encased steel castellated beams and the predicted values are in good agreement with the corresponding experimental values.

• Journal of Microbiology and Biotechnology
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• 제31권10호
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• pp.1331-1342
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• 2021

In this study, we evaluated the mechanism of long non-coding RNA MIR22 host gene (LncRNA MIR22HG) in osteosarcoma cells. Forty-eight paired osteosarcoma and adjacent tissues samples were collected and the bioinformatic analyses were performed. Target genes and potential binding sites of MIR22HG, microRNA (miR)-629-5p and tet methylcytosine dioxygenase 3 (TET3) were predicted by Starbase and TargetScan V7.2 and confirmed by dual-luciferase reporter assay. Cell Counting Kit-8, colony formation and flow cytometry assays were utilized to determine the viability, proliferation and apoptosis of transfected osteosarcoma cells. Pearson's analysis was introduced for the correlation analysis between MIR22HG and miR-629-5p in osteosarcoma tissue. Relative expressions of MIR22HG, miR-629-5p and TET3 were measured by quantitative real-time polymerase chain reaction or Western blot. MiR-629-5p could competitively bind with and was negatively correlated with MIR22HG, the latter of which was evidenced by the high expression of miR-629-5p and low expression of MIR22HG in osteosarcoma tissues. Overexpressed MIR22HG repressed the viability and proliferation but enhanced apoptosis of osteosarcoma cells, which was reversed by miR-629-5p upregulation. TET3 was the target gene of miR-629-5p, and the promotive effects of upregulated miR-629-5p on the viability and proliferation as well as its repressive effect on apoptosis were abrogated via overexpressed TET3. To sum up, overexpressed MIR22HG inhibits the viability and proliferation of osteosarcoma cells, which was achieved via regulation of the miR-629-5p/TET3 axis.

• Journal of Microbiology and Biotechnology
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• 제31권7호
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• pp.921-932
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• 2021

LINC01272 is a long non-coding RNA (lncRNA) that has been considered as a biomarker for many diseases including lung squamous cell carcinoma. Here, we investigated the function and mechanism of LINC01272 on lung cancer (LC). The differential expression of LINC01272 in LC and normal samples was analyzed by GEPIA based on the data from TCGA-LUAD database, as survival prognosis was analyzed through Kaplan-Meier Plotter. LINC01272 overexpression plasmid and miR-7-5p mimic were transfected into A549 and PC-9 cells. LINC01272, miR-7-5p and cardiolipin synthase 1 (CRLS1) mRNA expression was measured by quantitative reverse transcription-polymerase chain reaction. Cell viability was detected through MTT assay. Cell multiplication was evaluated by cell formation assay. Cell apoptosis was assessed through flow cytometry assay. Through bioinformatics, the target miRNA of LINC01272 and downstream genes of miR-7-5p were predicted. The targeting relationship was tested by dual luciferase reporter analysis. CRLS1, B-cell lymphoma-2 (Bcl-2), BCL2-associated X (Bax) and cleaved caspase-3 protein levels were detected through western blot. LINC01272 was downregulated in LC and low LINC01272 expression had poor prognosis. In A549 and PC-9 cells, LINC01272 inhibited cell viability and multiplication and induced apoptosis. LINC01272 negatively regulated miR-7-5p and CRLS1 was a target of miR-7-5p. MiR-7-5p reversed the effect of LINC01272 on viability, multiplication, apoptosis and expression of miR-7-5p and CRLS1 as well as apoptosis-related factors (Bcl-2, Bax and cleaved caspase-3). LINC01272 suppressed cell multiplication and induced apoptosis via regulating the miR-7-5p/CRLS1 axis in LC.

• Biomolecules & Therapeutics
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• 제29권2호
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• pp.195-204
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• 2021

Cereblon (CRBN), a substrate receptor of cullin 4-RING E3 ligase (CRL4) regulates the ubiquitination and degradation of c-Jun, mediating the lipopolysaccharide-induced cellular response. However, the upstream signaling pathway that regulates this process is unknown. In this study, we describe how endoplasmic reticulum (ER) stress reversely regulates sequestosome-1 (p62)and c-Jun protein levels. Furthermore, our study reveals that expression of p62 attenuates c-Jun protein levels through the ubiquitinproteasome system. Conversely, siRNA knockdown of p62 elevates c-Jun protein levels. Immunoprecipitation and immunoblotting experiments demonstrate that p62 interacts with c-Jun and CRBN to form a ternary protein complex. Moreover, we find that CRBN knockdown completely abolishes the inhibitory effect of p62 on c-Jun. Using brefeldin A as an inducer of ER stress, we demonstrate that the p62/c-Jun axis participates in the regulation of ER stress-induced apoptosis, and that CRBN is required for this regulation. In summary, we have identified an upstream signaling pathway, which regulates p62-mediated c-Jun degradation. Our findings elucidate the underlying molecular mechanism by which p62/c-Jun axis regulates the ER stress-induced apoptosis, and provide a new molecular connection between ER stress and apoptosis.

• Journal of Microbiology and Biotechnology
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• 제34권7호
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• pp.1511-1521
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• 2024

This study aimed to determine the function of LINC00511 in Nod-Like Receptor Pyrin Domain 3 inflammasome-mediated chondrocyte pyroptosis via the regulation of miR-9-5p and FUT 1. Chondrocyte inflammatory injury was induced by treating chondrocytes with LPS. Afterwards, the levels of IL-1β and IL-18, the expression of NLRP3, ASC, Caspase-1, and GSDMD, cell viability, and LDH activity in chondrocytes were assessed. LINC00511 expression in LPS-treated chondrocytes was detected, and LINC00511 was subsequently silenced to analyse its role in chondrocyte pyroptosis. The subcellular localization of LINC00511 was predicted and verified. Furthermore, the binding relationships between LINC00511 and miR-9-5p and between miR-9-5p and FUT1 were validated. LINC00511 regulated NLRP3 inflammasome-mediated chondrocyte pyroptosis through the miR-9-5p/FUT1 axis. LPS-treated ATDC5 cells exhibited elevated levels of inflammatory injury; increased levels of NLRP3, ASC, Caspase-1, and GSDMD; reduced cell viability; increased LDH activity; and increased LINC00511 expression, while LINC00511 silencing inhibited the NLRP3 inflammasome to restrict LPS-induced chondrocyte pyroptosis. Next, LINC00511 sponged miR-9-5p, which targeted FUT1. Silencing LINC00511 suppressed FUT1 by upregulating miR-9-5p. Additionally, downregulation of miR-9-5p or overexpression of FUT1 neutralized the suppressive effect of LINC00511 knockdown on LPS-induced chondrocyte pyroptosis. Silencing LINC00511 inhibited the NLRP3 inflammasome to quench Caspase-1-dependent chondrocyte pyroptosis in OA by promoting miR-9-5p and downregulating FUT1.

• Journal of Ecology and Environment
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• 제32권2호
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• pp.115-121
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• 2009

In order to elucidate the mechanisms affecting plant distributions in the reclaimed dredging area in the Gwangyang steelworks, in the Gwangyang Bay, Korea, we examined soil characteristics and plant distributions in four study sites and a control site in the study area. Desalination occurring along a gradient with increasing elevation, resulting in decrease of soil pH, EC, P, K, Cl, Ca, Mg, and salt and an increase in soil T-N, silt, clay contents. From site 1 (the lowest-elevation site) to site 5 (the highest-elevation site), halophytes decreased in abundance and nonhalophytes increased. The dominant species in each site were: Phragmites communis, Limonium tetragonum, and 12 additional species at site 1, Carex pumila, Suaeda japonica, and 15 additional species at site 2, Spergularia marina, Scirpus planiculmis, and 22 additional species at site 3, Miscantus sinensis, Lespedeza bicolor, and 26 additional species at site 4 and Pinus thunberii, Rhododendron mucronulatum, and 39 additional species at site 5, which resembled a naturally-occurring P. thinbergii community. Cluster analysis of the vegetation data matrix grouped the 35 plots into 5 major groups, and cluster analysis using the soil environment data matrix revealed 4 major groups. CCA of the floristic and environmental data matrix showed a positive relationship of SAR, EC, Na, Cl, and Ca, which are related to salt, in the $1^{st}$ axis and $2^{nd}$ axis, but negative relationships for altitude, organic contents, silt, and clay contents. Notably, plant species in the reclaimed dredging area that were separated along the $1^{st}$ axis showed strong relationships with factors that related to salt. Long-term exposure to natural rainfall in the reclaimed dredging area changed the soil characteristics, such as salinity. This change in soil characteristics might alter the SAR, which affects plant survival strategies in a given habitat. These results strongly indicated that factors related to salt and elevation play important roles in determining the overall plant distribution in the reclaimed dredging area.

• 대한한방부인과학회지
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• 제20권2호
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• pp.176-187
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• 2007

Purpose: Recent studies show that Skin Resistance Variability(SRV) could be related to the functions of Hypothalamus-Pituitary-Ovary(H-P-O) axis. 7-Zone-Diagnostic System is oriental medicine measuring system using SRV. To know the differences of SRV of normal and abnormal menstrual cycle groups, we research the SRV of the two groups. Methods: We measured SRV of two groups that were consist of 126 women who took CP-6000A test in Sangji Oriental Medical Hospital from Mar. 2004 to Feb. 2006. After detection of SRV, we performed correlation analysis by SPSS 12.0. Results: The SRV was measured twice in 7 areas. By the results of 1st trial on Factor AA of the SRV, the patient group's results were lower than normal group's results in all 7 areas. And there were remarkable differences in 1, 2, 3, 4, 5, 7 areas between two groups. By the results of 2nd trial on Factor AA of the SRV, the patient group's results were lower than normal group's results in all 7 areas similar to 1st trial results. And there were remarkable differences in 2, 3 areas between two groups. Conclusion: Comparing normal group with patient group, these results may reflect the relations of abnormal menstrual cycle and H-P-O axis functions. Because patient's results were lower than normal's in all 7 areas. And remarkable different results in 2, 3 areas were repeated by twice trial all. Further study will be needed.