• 제목/요약/키워드: Oxygen flow rate

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동물 세포 반응기에서의 초미세 통기법이 산소 전달 속도와 세포 생존율에 미치는 영향 (Effect of Microsparged Aeration on Oxygen Transfer Rate and Cell Viability in Mammalian Cell Culture Bioreactor)

  • 김정모;장건희;최춘순;김정회
    • 한국미생물·생명공학회지
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    • 제29권4호
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    • pp.240-247
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    • 2001
  • 본 연구에서는 동물세포 배양장치를 개발키 위한 기초연구로서 초미세 통기법이 산소 전달 속도와 세포의 생존율에 미치는 영향에 대해 알아보았다. 통기 장치로 통기 구멍 크기가 다른 microsparger 를 사용하였을 때, 모형 반응기 내에서 측정한 산소 전달계수(k$_{L}$a)는 microsprager의 통기 구멍 크기가 작아질수록 현저히 증가하였다. 이는 공기 방울들과 매질 사이의 접촉 면적이 증가했기 때문인 것으로 판단된다. 두 가지 다른 형태의 임펠러 (square-pitch marine impeller 와 $45^{\circ}$) pitched flat blade impller) 를 사용하여 교반하였을 때, $k_{L}$ a 값은 marine impeller 를 사용하였을 때 다소 높았다. $100\mu\textrm{m}$ 이하의 통기 구멍을 가진 microsparger 를 사용하여 직접 통기가 세포에 미치는 손상에 대해 알아본 결과, 세포들의 손상 정도는 통기 속도가 증가할수록, 공기방울 크기가 작아질수록 더 커졌다. 2.5 L 용량의 소형 세포 반응기에 $0.5\mu\textrm{m}$ 의 통기 구멍 크기를 가진 micro-sparger를 장치하여 세포를 배양한 결과 , 지속적인 통기시에는 세포의 생존율이 80% 이하로 떨어지고, 정상적인 성장을 하지 못하였다. 그러나 용존 산소 농도가 20% 이하로 떨어졌을 때에만 통기하였을 때 세포는 정상적으로 자랐으며 세포 생존율도 대수기 전반에 걸쳐 90% 이상을 유지하였다.

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유량변동에 따른 탐진 A와 B유역에서의 오염물질 유출 특성 (Characteristics of the Pollutants Ronoff on the Tamjin A and B Watershed with Discharge Variation)

  • 박진환;임병진;정재운;김대영;오태윤;이동진;김갑순
    • 환경영향평가
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    • 제21권6호
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    • pp.917-925
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    • 2012
  • In this study, we report the runoff characteristics of pollutants for Tamjin A and B watershed in Tamjin river basin using statistical analysis, such as correlation analysis and regression equation. Flow rate and water qualtiy data collected from 2 sampling sites(Tamjin A and B watershed) during 3 years(2009~2011) were analyzed for biochemical oxygen demand(BOD), total nitorgen(TN), total phosphorus(TP) and suspended solid(SS). The results showed that strong correlations were observed between flow rate and SS in Tamjin A, while weak correlations were observed among the BOD, TN, and TP. In Tamjin B, strong correlations were observed among the flow rate, SS and T-P except BOD and TP. Meanwhile, the values of $R^2$ for regression equations between flow rate and pollutants load were greater than 0.7. Results of these statistics indicated that there was a good agreement between flow rate and pollutants load. Also, the flow rate exponents of regression equations for BOD, TN, and TP were smaller than 1 in Tamjin A. In Tamjin B, flow rate exponents of regression equation for BOD and TP were smaller than 1. These results indicated that concentrations of BOD, TN, TP in Tamjin A and concentrations of BOD and TP were decreased as the flow rate was increased. This means that rater than nonpoint sources, point sources affect BOD, TN and TP in Tamjin A and BOD and TP in Tamjin B.

화염편 모델을 이용한 하이브리드 로켓의 연소과정 해석 (Flamelet Modeling for Combustion Processes of Hybrid Rocket Engine)

  • 임재범;김용모;윤명원
    • 유체기계공업학회:학술대회논문집
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    • 유체기계공업학회 2006년 제4회 한국유체공학학술대회 논문집
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    • pp.245-248
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    • 2006
  • Hybrid propulsion systems provide many advantages in terms of stable operation and safety. However, classical hybrid rocket motors have lower fuel regression rate and combustion efficiency compared to solid propellant rocket motor. Accordingly, the recent research efforts are focused on the improvement of engine efficiency and regression rate in the hybrid rocket engine. The present study has numerically investigated the combustion processes in the hybrid rocket engine. The turbulent combustion is represented by the flamelet model and Low Reynolds number $k-{\varepsilon}$ turbulent model is employed to reduce the uncertainties for convective heat transfer near solid fuel surface having strong blowing effect. Based on numerical results, the detailed discussions have been made for the effects of oxygen injection methods and oxygen injection flow rate on flame structure and regression rate in the vortex hybrid rocket engines

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유연 기판 위에 증착된 IZO 박막의 구조적 및 전기적 특성 (Structural and electrical characteristics of IZO thin films deposited on flexible substrate)

  • 이봉근;이규만
    • 반도체디스플레이기술학회지
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    • 제10권2호
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    • pp.39-44
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    • 2011
  • In this study, we have investigated the structural and electrical characteristics of IZO thin films deposited on flexible substrate for the OLED (organic light emitting diodes) devices. For this purpose, PES was used for flexible substrate and IZO thin films were deposited by RF magnetron sputtering under oxygen ambient gases (Ar, $Ar+O_2$) at room temperature. In order to investigate the influences of the oxygen, the flow rate of oxygen in argon mixing gas has been changed from 0.1sccm to 0.5sccm. All the samples show amorphous structure regardless of flow rate. The electrical resistivity of IZO films increased with increasing flow rate of $O_2$ under $Ar+O_2$. All the films showed the average transmittance over 85% in the visible range. The OLED device was fabricated with different IZO electrodes made by configuration of IZO/a-NPD/DPVB/$Alq_3$/LiF/Al to elucidate the performance of IZO substrate. OLED devices with the amorphous-IZO (a-IZO) anode film show better current density-voltage-luminance characteristics than that of OLED devices with the commercial crystalline-ITO (c-ITO) anode film. It can be explained that very flat surface roughness and high work function of a-IZO anode film lead to more efficient hole injection by reduction of interface barrier height between anode and organic layers. This suggests that a-IZO film is a promising anode materials substituting conventional c-ITO anode in OLED devices.

고효율 질화갈륨계 발광 다이오드용 전자선 증착 ITO 투명 전도 전극 연구 (Electron Beam Evaporated ITO Transparent Electrode for Highly Efficiency GaN-based Light Emitting Diode)

  • 서재원;오화섭;강기만;문성민;곽준섭;이국회;이우현;박영호;박해성
    • 대한금속재료학회지
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    • 제46권10호
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    • pp.683-690
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    • 2008
  • In order to develop transparent electrodes for high efficiency GaN-based light emitting diodes (LEDs), the electrical and optical properties of the electron beam evaporated ITO contacts have been investigated as a function of the deposition temperature and flow rate of oxygen during the deposition. As the deposition temperature increases from $140^{\circ}C$ to $220^{\circ}C$, the resistivity of the ITO films decreases slightly from $4.0{\times}10^{-4}{\Omega}cm$ to $3.3{\times}10^{-4}{\Omega}cm$, meanwhile the transmittance of the ITO films significantly increases from 67% to 88% at the wavelength of 470 nm. When the flow rate of oxygen during the deposition increases from 2 sccm to 4 sccm, the resistivity of the ITO films increases from $3.6{\times}10^{-4}{\Omega}cm$ to $7.4{\times}10^{-4}{\Omega}cm$, meanwhile the transmittance of the ITO films increases from 86% to 99% at 470 nm. Blue LEDs fabricated with the electron beam evaporated ITO electrode show that the ITO films deposited at $200^{\circ}C$ and 3 sccm of the oxygen flow rate give a low forward-bias voltage of 3.55 V at injection current of 20 mA with a highest output power.

분리막과 PSA혼합법에 의한 고순도 산소의 제조 (Production of High Purity Oxygen by Combination of Membrane and PSA Methods)

  • Hwang, Sun-Tak
    • 멤브레인
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    • 제4권1호
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    • pp.1-8
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    • 1994
  • There are growing needs to produce relatively high purity(99.0% or higher) oxygen at low cost. For small scale production, both pressure swing adsorption(PSA) and membrane process are competitive and less expensive or more convenient than well known cryogenic fractionation technology. A continuous membrane column(CMC) combined with a PSA oxygen generator can be employed to produce high purity oxygen continuously. The oxygen enriched gas generated by a PSA unit, with a concentration of 93~94%, is fed to the CMC that consism of three modules of poly(imide) hollow fibers. Several experiments were conducted by varying parameters, such as feed flow rate, transmembrane pressure drop, stage cut, and feed location in order to obtain a high oxygen concentration above 99.0%. A two-series unit mode was also employed with CMC operation to optimize the given membrane area.

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Removal of Dissolved Oxygen from the Make-up Water of NPP Using Membrane-based Oxygen Removal System

  • Chung, Kun-Ho;Kang, Duck-Won;Hong, Sung-Yull
    • Nuclear Engineering and Technology
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    • 제31권6호
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    • pp.541-547
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    • 1999
  • Corrosion control, in the end-shield cooling system of Wolsung Nuclear Power Plant, is directly related to the control of dissolved oxygen (DO). The current method, being used to deoxygenate the end-shield cooling water, is a chemical treatment by addition of reducing agent, hydrazine, to react with DO. This method has several limitations including high reaction temperature of hydrazine , unwanted explosive hydrogen gas production, and its intrinsic harmful property. A new approach to remove DO using a membrane-based oxygen removal system (MORS) was tried to overcome limitations of the hydrazine treatment. The DO removal efficiency of the MORS was found to be in the range 87% to 98%: The higher vacuum, the lower water flow rate and the higher water temperature tend to increase the DO removal efficiency.

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Production of High Purity Oxygen by Conbination of Membrane and PSA Methods

  • Hwang, Sun-Tak
    • 한국막학회:학술대회논문집
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    • 한국막학회 1994년도 심포지움시리즈 Jan-94 기체분리막 기술 및 응용
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    • pp.1-21
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    • 1994
  • There are growing needs to produce relatively high purity (99.0% or higher) oxygen at low cost. For small scale production, both pressure swing adsorption (PSA) and membrane process are competitve and less expensive or more convient than well known crygenic fractionation technology. A continuous membrane colume (CMC) combined with a PSA oxygen generator can be employed to produce high purity oxygen continuosly. The oxygen-enriched gas generated by a PSA unit, with a concentration of 93-94%, is fed to the CMC that consists of three modules of poly(imide) hollow fibers. Several experiments were conducted by varying parameters, such feed flow rate, transmenbrane pressure drop, stage cut, and feed location in order to obtain a high oxygen concentration above 99.0%. A two-series unit mode was also employed with CMC operation to optimize the given membrane area.

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Sap Temperature Distribution of the Xylem and Leaf Water Status of Apple Trees in Relation to Soil Oxygen Diffusion Rates

  • Ro, Hee-Myong
    • Journal of Applied Biological Chemistry
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    • 제43권3호
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    • pp.170-175
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    • 2000
  • A pot-lysimeter experiment was conducted with 3-year-old 'Tsugaru' apple (Malus domestica Borkh) trees to examine the changes in oxygen diffusion rate (ODR) with lateral flow velocity of water through soil. The influence of lateral water flow velocity on water relations and elemental content in leaf, and sap temperature distribution patterns of the xylem of trees were also determined. Trees were grown under four soil water regimes: (1) fast laterally flowing (FWT, $2.50{\times}10^{-4}cm\;s^{-1}$), (2) slow laterally flowing (SWT, $0.25{\times}10^{-4}cm\;s^{-1}$), and (3) stagnant water table (WLT) at 60-cm, and (4) drip-irrigation at -40 kPa of soil matric potential as a control. The rate of $O_2$ diffusion converged near $2{\times}10^{-3}g\;m^{-2}\;min^{-1}$ for FWT and control soils, but decreased below $1{\times}10^{-3}g\;m^{-2}\;min^{-1}$ 40 days after treatment (DAT) for WLT soils. For SWT soils, however, the ODR at 15 cm below the soil surface was similar to that of control, but at 45 cm below the soil surface, ODR was similar to that of the WLT treatment. Leaf water potential of FWT and SWT plants was similar to that of control plants, but the values for SWT plants declined by 98 DAT. Leaf water potential of WLT plants decreased from -1.86 MPa (9 DAT) to -2.41 MPa (59 DAT) and finally down to -2.70 MPa. The sap temperature measured at 1100-hr was lowest at top and highest at bottom for FWT and control plants, but this pattern of SWT and WLT plants was disturbed from 29 DAT. However, for SWT plants, such thermal disturbance of sap temperature disappeared from 63 DAT.

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회전하는 산소전달장치가 부착된 동물세포 배양기의 조업 성능에 관한 고찰 (Performance of a Novel Bioreactor Equipped with Moving Membrane Tube-Aeration System)

  • 김영남;전병철;이진하;이현용
    • 한국미생물·생명공학회지
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    • 제21권4호
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    • pp.348-353
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    • 1993
  • The optimal conditions for operating a moving-aeration bioreactor were determined as 30rpm and 150 (ml/min) of air flow rate, which can yield ca. 7.3 (l/h)of maximum mass transfer coefficient. It was also found that the agitation speed played much much important role than air input rate in oxgen transfer into the medium. $2.6{\times}10^6$ (cells/ml) and 0.6 (ml/l) of maximum cell denisty and IL-2 production were observed in batch cultivation of IL-2 producing BHK cell line. 0.53 (mM/l/h) of oxygen uptake rate was also estimated. The performance of a moving-aeration bioreactor (specific growth rate and oxygen uptake rate, etc.) was superior to other culture systems, such as cell-life and static membrane aeration bioreactors. Ii must be useful to apply this reactor to many culture processes by improving structural limitations in scaling-up the system.

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