• Journal of Periodontal and Implant Science
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• 제44권2호
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• pp.57-64
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• 2014

Purpose: Both chronic periodontitis (CP) and iron deficiency anemia (IDA) induce oxidative stress in the body and cause an imbalance between reactive oxygen species and antioxidants, such as superoxide dismutase (SOD). This study explored the SOD enzyme activity of saliva and serum in CP patients with and without IDA and analyzed the impact of IDA on CP. Methods: A total of 82 patients were divided into four groups: control group (CG, 22), periodontally healthy IDA patients (IDA-PH, 20), CP patients (CP, 20), and IDA patients with CP (IDA-CP, 20). After clinical measurements and samplings, serum and salivary SOD levels were determined using an SOD assay kit. Results: IDA-CP patients exhibited a higher gingival index, bleeding on probing, probing pocket depth, and percentage (%) of sites with a clinical attachment loss (CAL) of ${\geq}6mm$ (P<0.008) than CP patients. The mean salivary and serum SOD levels were significantly lower in the IDA-PH, CP, and IDA-CP patients than in the CG group (P<0.008). A significant positive correlation between salivary and serum SOD activity was observed in IDA (P<0.05). Furthermore, serum and salivary SOD levels were significantly and negatively correlated with all periodontal parameters including the percentage of sites with CAL of 4-5 and ${\geq}6mm$ (P<0.05) except the significant correlation between salivary SOD activity and mean CAL and the percentage of sites with CAL of 4-5 mm (P>0.05) in these patients. Conclusions: Within the limits of this study, it may be suggested that IDA patients with chronic periodontitis have more periodontal breakdowns than patients with chronic periodontitis. Serum and salivary SOD activity levels were lower in the IDA-PH, CP and IDA-CP groups than in the CG. Iron deficiency anemia influenced the serum SOD activity but did not seem to affect the salivary SOD activity in these patients.

• 한국작물학회지
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• 제50권3호
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• pp.170-174
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• 2005

본 연구에서는 보리 습해의 원인인 과습에 의한 근권의 산소부족이 보리의 뿌리와 지상부의 amylase 및 sucrose synthase활성에 미치는 영향을 배양액의 용존산소를 $1\~2ppm$의 혐기조건으로 1-7일간 처리한 3엽기 보리 유묘를 이용하여 조사하였다. 해당과정의 재료인 glucose의 공급에 관여하는 효소 중에서 전분을 분해하는 $\alpha-amylas$의 활성은 혐기조건에서 증가하나 자당을 분해하는 sucrose synthase활성은 혐기조건에서도 호기적 상태의 수준을 유지하였다. 이러한 결과는 에너지 획득과정에서의 $\alpha-amylas$의 역할이 호기조건에서보다 혐기조건에서 상대적으로 증대하는 것을 의미하는 것으로 사료된다.

• 한국가스학회지
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• 제15권3호
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• pp.26-30
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• 2011

개방형 순간 가스온수기는 설치와 관리가 적절히 되지 않아 일산화탄소 중독과 질식과 같은 인명사고를 발생시킬 수 있다. 연소기에 공기가 충분히 공급되지 않거나 배기구가 막히면 배기가스 중의 일산화탄소(CO)의 농도가 증가하는 특성을 보인다. 본 연구는 가정집에서 사용 중인 개방형 가스온수기를 회수하여 연소기 주변 공기 중산소농도 및 배기가스 중 CO농도의 변화를 확인하였다. 환기구 면적에 따른 산소농도 측정 실험에서 환기구의 면적이 바닥면적의 3.5%에서 산소농도가 약 17.7%로 나타났고, 배기구를 폐쇄한 상태에서 CO농도를 측정한 결과 4,000ppm이상이 측정되었다. 따라서 정기적으로 배기구를 점검하는 것이 환기구 크기보다 일산화탄소 중독사고를 예방하기 위하여 중요하다.

• 한국미생물·생명공학회지
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• 제19권1호
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• pp.76-81
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• 1991

2l 발효조에서 pH6.9, 온도 $32^{\circ}C$일 때 당밀배지를 이용하여 Corynebacterium glutamicum의 영양요구성 유사체 내성변이주에 의한 라이신 발효시 산화환원 전위 (ORP)가 라이신 발효속도의 특성에 미치는 영향을 조사하였다. 희석률이 0.1$h ^1$일때 탄소원이 제한되건 로이신이 제한되건 산소가 제한되지 않는 한 최대의 대당수율 24를 보였으며, 이 때의 산화환원 전윈 값은 -60mV와 -100mV 범위에 해당하였다. 산화화원 전위 값이 -130mV의 매우 낮은 용존산소 조건하에서는 대당수율 밀 $q_s, q_p$ 등의 발효 반응속도 상수값들이 크게 감소하였으며 glvcine, alanine, valine을 포함하는 발효 부산물의 축적량이 매우 높아졌다.

• Clinical and Experimental Reproductive Medicine
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• 제41권3호
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• pp.125-131
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• 2014

Objective: Under estrogen deficiency, blastocysts cannot initiate implantation and enter dormancy. Dormant blastocysts live longer in utero than normal blastocysts, and autophagy has been suggested as a mechanism underlying the sustained survival of dormant blastocysts during delayed implantation. Autophagy is a cellular degradation pathway and a central component of the integrated stress response. Reactive oxygen species (ROS) are produced within cells during normal metabolism, but their levels increase dramatically under stressful conditions. We investigated whether heightened autophagy in dormant blastocysts is associated with the increased oxidative stress under the unfavorable condition of delayed implantation. Methods: To visualize ROS production, day 8 (short-term dormancy) and day 20 (long-term dormancy) dormant blastocysts were loaded with $1-{\mu}M$ 5-(and-6)-chloromethyl-2', 7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-$H_2DCFDA$). To block autophagic activation, 3-methyladenine (3-MA) and wortmannin were used in vivo and in vitro, respectively. Results: We observed that ROS production was not significantly affected by the status of dormancy; in other words, both dormant and activated blastocysts showed high levels of ROS. However, ROS production was higher in the dormant blastocysts of the long-term dormancy group than in those of the short-term group. The addition of wortmannin to dormant blastocysts in vitro and 3-MA injection in vivo significantly increased ROS production in the short-term dormant blastocysts. In the long-term dormant blastocysts, ROS levels were not significantly affected by the treatment of the autophagy inhibitor. Conclusion: During delayed implantation, heightened autophagy in dormant blastocysts may be operative as a potential mechanism to reduce oxidative stress. Further, ROS may be one of the potential causes of compromised developmental competence of long-term dormant blastocysts after implantation.

• 한국작물학회지
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• 제49권4호
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• pp.284-288
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• 2004

The response of dissolved oxygen (DO) concentrations caused significant change in root number, root length, coleoptile length, shoot length and leaf age of seedlings. The genotypic difference in the effect of DO also highly significant (P<0.01) for all of the seedling traits. The number and length of root were extremely inhibited at the condition of $0.39\pm0.09$ DO concentration. While the coleoptile elongated markedly in the lowest DO concentrations, the shoot did not develop. The root growth was improved slightly at the $1.39\pm0.27mg L^{-1}$, however, there were no difference among genotypes at these two low DO concentrations. As the DO concentration become higher, the growth of root and shoot was improved remarkably. Root number, root length and shoot length was significantly different between $20\;and\;30^{\circ}C$ in DO rich and normal conditions, the development of those traits were apparently accelerated in high water temperature, however those traits of seedlings in DO deficiency were not different between the two temperatures except for shoot length. On the other hand the coleoptile length was not affected by the stagnant water temperature; it was stimulated by the low DO concentration. The competition of DO was greater as the seedling density was increased in the stagnant water, therefore the seedlings grown under high density have long and white coleoptiles, and the growth of roots and shoots was retarded severely.

• International Journal of Oral Biology
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• 제41권1호
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• pp.17-23
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• 2016

Chronic/cyclic neutropenia, leukocyte adhesion deficiency syndrome, Papillon-$Lef{\grave{e}}vre$ syndrome, and $Ch{\grave{e}}diak$-Higashi syndrome are associated with severe periodontitis, suggesting the importance of neutrophils in the maintenance of periodontal health. Various Toll-like receptor (TLR) ligands are known to stimulate neutrophil function, including FcR-mediated phagocytosis. In the present study, the effect of TLR2 activation on the non-opsonic phagocytosis of oral bacteria and concomitant production of reactive oxygen species (ROS) by human neutrophils was evaluated. Neutrophils isolated from peripheral blood were incubated with Streptococcus sanguinis or Porphyromonas gingivalis in the presence of various concentrations of $Pam_3CSK_4$, a synthetic TLR2 ligand, and analyzed for phagocytosis and ROS production by flow cytometry and chemiluminescence, respectively. $Pam_3CSK_4$ significantly increased the phagocytosis of both bacterial species in a dose-dependent manner. However, the enhancing effect was greater for S. sanguinis than for P. gingivalis. $Pam_3CSK_4$ alone induced ROS production in neutrophils and also increased concomitant ROS production induced by bacteria. Interestingly, incubation with P. gingivalis and $Pam_3CSK_4$ decreased the amounts of ROS, as compared to $Pam_3CSK_4$ alone, indicating the possibility that P. gingivalis survives within neutrophils. However, neutrophils efficiently killed phagocytosed bacteria of both species despite the absence of $Pam_3CSK_4$. Although P. gingivalis is poorly phagocytosed even by the TLR2-activated neutrophils, TLR2 activation of neutrophils may help to reduce the colonization of P. gingivalis by efficiently eliminating S. sanguinis, an early colonizer, in subgingival biofilm.

• 한국수산과학회지
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• 제27권2호
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• pp.200-214
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• 1994

진해만의 저서동물 군집생태 연구의 일환으로 저서환경의 특성을 구명하기 위하여, 1987년 6월부터 1990년 5월까지 12개 정점에서 표층 및 저층수의 수온, 염분, 용존산소와 퇴적물의 입도조성 및 유기물량을 조사하였다. 진해만의 저서환경은 내만으로 들어 갈수록 퇴적물 입도의 세립화, 높은 유기물함량, 여름철 성층의 발달로 인한 저층 빈산소 수괴의 형성 등이 특징적으로 나타났다. 저층의 빈산소수괴는 5월부터 내만에서 형성되기 시작하여, 여름철로 접어들면서 점차 외해역으로 확산되어 9월에 최고에 달하였으며, 진해만 전 해역의 절반이상이 빈산소 영향을 받고 있는 것으로 나타났다. 이러한 저층의 빈산소 환경은 가을철로 접어들면서 성층의 점진적 소멸과 함께 회복된다. 저층수의 수온, 염분 및 용존 산소량과 퇴적물의 입도 및 유기물 함량의 5개 환경요인에 대하여 주성분 분석을 실시한 결과 4개 해역으로 나눌 수 있었으며, 이러한 공간 분포는 전반적으로 퇴적물의 유기물함량과 가장 높은 상관관계를 가지는 것으로 나타났다.

• 한국양식학회지
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• 제10권4호
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• pp.457-462
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• 1997

1997년 9월 1일과 2일 심한 적조가 발생된 해역의 가두리 양식어장에서 적조원인 생물인 Cochlodinium polykrikoides의 주야 수직이동을 세포수와 Chlorophyll a로 분석한 결과 낮에는 표층에서 4 m까지 농밀층이 분포하고 있었으나 저녁에는 저층 10 m, 15 m층에 주로 분포하였다. 이로 볼 때 황토를 이용한 적조방제는 저녁 18시부터 20시까지, 아침 8시부터 10시까지가 눈에 보이지 않는 위험시기이다. 조사 당시 가두리 주위에 황토를 12시부터 18시까지 연속적으로 뿌려 적조를 방제하였으나 가두리 주위에서 용존산소의 분포는 정상이었다. 이때 수질환경 중 pH는 8.45로 정상치보다 높았으며 영양염 중 암모니아와 인산은 군집유지에 효율적으로 이용되었고, 질산염과 규산염은 정상보다 다소 높은 농도를 나타내었다.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2016년도 추계학술대회 논문집
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• pp.59-75
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• 2016

Reduced or black $TiO_{2-x}$ materials with oxygen-deficiency have been achieved by creating oxygen vacancies and/or defects at the surface using different methods. Fascinatingly, they exhibited an extended absorption in VIS and IR instead of only UV light with bandgap decrease from 3.2 (anatase) to ~1 eV. However, despite the dramatic enhancement of optical absorption in black $TiO_{2-x}$ materials, they have failed to show expected visible light-assisted water splitting efficiency. This was ascribed to the high concentration of the surface defects and/or oxygen vacancies, considered as an electron donor to enhance donor density and improve the charge transportation in black $TiO_2$ can also act as charge recombination centers, which eventually decrease photocatalytic activity. Therefore, a black ot reducd $TiO_2$ material with optimized properties would be highly desired for visible light photocatalysis. In this report, a new controlled magnesiothermic reduction has been developed to synthesize reduced black $TiO_{2-x}$ in the presence $H_2/Ar$ for photocatalytic $H_2$ production from methanol-water system. The material possesses an optimum band gap and band position, oxygen vacancies, and surface defects and shows significantly improved optical absorption in the visible and infrared region. The synergistic effects enable the reduced $TiO_{2-x}$ material to show an excellent hydrogen production ability along with long-term stability under the full solar wavelength range of light and visible light, respectively, in the methanol-water system in the presence of Pt as a co-catalyst. These values are superior to those of previously reported black $TiO_2$ materials. On the basis of all the results, it can be realized that the outstanding activity and stability of the reduced of $TiO_{2-x}$ NPs suggest that a balanced combination of different factors like $Ti^{3+}$, surface defects, oxygen vacancy, and recombination center is achieved along with optimized bandgap and band position during the preparation employing magnesiothermic reduction in the presence of $H_2$. The controlled magnesiothermic reduction in the presence of $H_2$ is one of the best alternative ways to produce active and stable $TiO_2-based$ photocatalyst for $H_2$ production.