• Journal of Food Hygiene and Safety
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• v.30 no.2
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• pp.166-172
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• 2015

This study was performed to develop treatment method for reducing microbial contamination on Daruma (a semi-processed product of seasoned and dried squid) by combination of strong acidic hypochlorous water (SAHW) and ultrasonic waves (UW). The available chlorine concentration, oxidation reduction potential (ORP) and pH of SAHW were $69.67{\pm}0.58ppm$, $1071.33{\pm}4.16mV$ and 2.79, respectively. The 1.49 log CFU/g of viable cell count and 1.32 log CFU/g of Staphylococcus aureus was reduced, and Escherichia coli was reduced below detection limit when the Daruma was treated with 20 times (w/v) of sodium hypochlorite solution (SHS) for 120 min. The 3.62 log CFU/g of viable cell count and 3.22 log CFU/g of Staphylococcus aureus was reduced, and Escherichia coli was reduced below detection limit when the Daruma was treated with 20 times (w/v) of SAHW for 120 min. The antibacterial effects of SAHW were stronger than those of SHS at same available chroline concentration. SAHW treatment after washing strongly alkalic electrolyzed water (SAEW) showed better bactericidal effects than SAHW treatment only. The 4.0 log CFU/g of viable cell count was reduced, S. aureus was reduced below regulation limit (Log 2.0 CFU/g), and E. coli was reduced below detection limit when the Daruma was treated with 20 times (w/v) of SAHW for 90 min after washing with 20 times (w/v) of SAEW for 60 min. The viable cell number was reduced below detection limit and S. aureus was reduced below regulation limit when the Daruma was treated with 20 times (w/v) of SAHW for 60 min in ultrasonic washer. E. coli was reduced below detection limit when the Daruma was treated with 20 times (w/v) of SAHW for 10 min in ultrasonic washer. These results suggest that combination of SAHW and UW may be a good technique to reduce the microbial contamination in daruma.

• Journal of Nutrition and Health
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• v.50 no.3
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• pp.225-235
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• 2017

Purpose: This study aimed to investigate the potential of freeze-dried persimmon powder (Diospyros kaki Thumb.) to protect against dyslipidemia induced by a high-fat/cholesterol diet (HFD) in a rat model. Methods: Fifty Wistar rats were randomly divided into five groups: normal control (NC), high-fat/cholesterol control (HC), tannin in HFD (HT, 1% of diet), immature persimmon in HFD (HI, 7% of diet), and mature persimmon in HFD (HM, 7% of diet). Tannin was used as a positive control. Biochemical, molecular, and histopathological changes were observed in the blood and liver. Results: We confirmed that a high fat/cholesterol diet successfully induced dyslipidemia, which was characterized by significantly altered lipid profiles in the plasma and liver. However, oxidized low-density lipoprotein levels, histopathological damage in the liver, and hepatic triglyceride levels were significantly reduced in all HT, HI, and HM groups compared to those in the HF group. In contrast, plasma apolipoprotein B level was significantly reduced only in the HT and HM groups, whereas reduction of the LDL-C level was detected only in the HI group. Although HF-induced sterol regulatory element-binding protein (SREBP) gene expression was significantly reduced in all treated groups, downstream gene expression levels varied among the different groups; significant reduction of fatty acid synthase (FAS) and 3-hydroxy-3-methylglutaryl-CoA (HMGCR) gene expression was detected only in the HI group, whereas cholesterol $7{\alpha}$-hydroxylase (CYP7A1) gene expression was significantly elevated only in the HM group. Conclusion: Taken together, the data suggest that protection of LDL oxidation and hepatic lipogenesis might be, at least partly, attributed to tannin in persimmons. However, the identified mechanisms varied up to the maturation stage of persimmon. In the case of immature persimmon, modulation of FAS and HMGCR gene expression was prominent, whereas in the case of mature persimmon, modulation of CYP7A1 gene expression was prominent.

• Journal of Life Science
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• v.20 no.7
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• pp.1107-1112
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• 2010

In this study, the stable maintenance of bioactivity in alkaline ionized water (AIW) and antibacterial effects of AIW were evaluated to confirm benefits of AIW. As controls, purified water (PW) and tap drinking water (DW) were used. The pH and ORP (oxidation-reduction potential) of AIW, PW and DW used were 9.5 and 120 mV, 7.2 and 144 mV, and 7.3 and 564 mV, respectively. High level of minerals was observed in DW (DW>AIW>PW of mineral contents). Concentrations of $Ca^{++}$ and $Na^+$ in DW were 14.5, and 8.4 mg/l, respectively, while no $Ca^{++}$, $Mg^{++}$, $K^+$, and $Na^+$ were detected in PW. Evaluation of antioxidant activities for AIW, PW and DW showed that the waters did not act as antioxidants. However, the DPPH (1,1-diphenyl-2-picryl hydrazyl) or superoxide radical scavenging activities or reducing power of vitamin C were stably maintained in AIW and PW, though not in DW, against heat treatment ($60^{\circ}C$) or vigorous shaking (120 rpm) at $37^{\circ}C$. Similarly, after aspirin treatment at $60^{\circ}C$ for 1 hr, the antithrombosis activity in PW and AIW was 62.6% and 55.3%, while that of DW was 52.1%. Furthermore, cell growth analysis and viable cell count of Escherichia coli H7:O157 in PW, AIW and DW showed that AIW and DW, not DW, have antibacterial activities. Our results suggest that the state of water, for example pH, ORP and mineral contents of water, should be considered in medicine or food industries, and that AIW has high potential for utilization in various fields.

• Applied Biological Chemistry
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• v.17 no.3
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• pp.193-218
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• 1974

These studies were carried out for the elucidation of liming effect on the growth of rice seedlings and the chemical characteristics of an acid sulphate paddy that shows not only extremely high acidity of soil but also poor growth of rice plants, consequently low yield. Thus the liming effect on the changes of acidity, oxidation-reduction potential, and the contents of iron, aluminium, sulphate, and phosphorus fractions in the soil was investigated under the waterlogging and drying condition. The reclaimable or inhibitory effect of phosphorus, iron and aluminium on the growth of rice seedlings was also investigated under liming. The results are summarized as follows: 1. After liming, the pH of the acid sulphate subsoil decreased again on drying. 2. The oxidation-reduction potential reached a minimum after 5 days of flooding and greatly decreased on liming but increased after drying. 3. The contents of ferrous iron soluble in water-and Morgan's solution reached a maximum after 15 days of flooding and only the content of water soluble ferrous iron was greatly decreased. 4. The content of aluminium soluble in water-and Morgan's solution decreased by flooding and liming, and showed a tendency to increase on drying. 5. In the limed acid sulphate soil, the content of water soluble calcium showed a highly significant negative correlation with the content of sulphate and liming decreased sulphate content in the soil. 6. The contents of total phosphorus was 496.3 ppm in the acid sulphate topsoil and 387.5 ppm in the subsoil. The content of each phosphorus fraction was in the order of Fe-P>Occ. Fe-P>Ca-P>Occ. Al-P>Al-P and Fe-P content in the soil was the highest fraction among them. 7. Lime application increased greatly Ca-P and Al-P, and Occ. Fe-P and Occ. Al-P only slightly, but decreased Fe-P differently in each soil. 8. Effect of phosphorus on the dry matter yield of rice seedlings was great. The optimum amount of phosphorus to produce maximum dry matter yield of rice seedlings appeared to be 6.8% of maximum absorption (absorption coefficient) without liming and 10.0% with liming. 9. In rice seedlings liming increased the content and uptake of calcium and silica but decreased those of iron and aluminium. Phosphorus application increased the content and uptake of phosphorus and decreased iron while the application of iron and aluminium increased their contents and uptake but decreased those of phosphorus. 10. Liming greatly alleviated such toxicity of iron and aluminium. 11. When phosphorus was applied, the dry matter yield of rice seedlings showed highly significant positive correlations with uptake of phosphorus, calcium and silica each. When iron and aluminium were applied, dry matter yields indicated significant positive correlations with the contents or uptake of calcium and silica each, but significant negative correlations with the content or uptake of iron and aluminium. 12. Under the application of phosphorus and lime, dry matter yields showed significant positive correlations with pH and Morgan's extractable calcium each of the soil samples after harvest. Under the application of lime, iron and aluminium, dry matter yields showed significant positive correlations with pH, calcium and silica each, but negative correlations with iron and aluminium contents each of the soil samples after harvest.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.5
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• pp.776-781
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• 2010

The properties and bactericidal activities of strongly acidic electrolyzed water (SEW) against food-borne pathogenic bacteria, vegetables and kitchen apparatuses were investigated. The available chlorine concentration, pH and oxidation reduction potential (ORP) of SEW were $35{\pm}1.2\;ppm$, $2.3{\pm}0.2$, and $1,140{\pm}20.4\;mV$, respectively. Five strains of food-borne bacteria with initial cell number of 7.00 log CFU/mL were not detected except Bacillus cereus after treatment with SEW for 60 sec. The numbers of Bacillus cereus were reduced to 2.08{\pm}1.00 log CFU/mL at the same condition. In vegetables, SEW treatment after washing strongly in alkalic electrolyzed water (AEW) showed better bactericidal effects than SEW only. The viable cell on stainless steel bowl ($3.86{\pm}2.49\;\log\;CFU/100\;cm^2$) and cup for water ($2.40{\pm}1.80\;\log\;CFU/100\;cm^2$) were not detected by SEW treatment (35 ppm of available chlorine concentration) for 30 sec, but survival of more than 1.00 log CFU/$100\;cm^2$ of viable cell was shown by washing of sodium hypochlorite solution at the same condition. On the other hand, the coliform group bacteria ($5.08{\pm}4.00\;CFU/100\;cm^2$) were detected on rubber globe only, and more than 2.00 log CFU/$100\;cm^2$ of viable cell and coliform group bacteria on it survived, though it was washed with flowing SEW for 30 sec.

• Korean Journal of Food Science and Technology
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• v.38 no.6
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• pp.742-750
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• 2006

This study was carried out to investigate the efficacy of two kinds of electrolyzed water with added 0.5% (v/v) citron juice, SAEW-CJ[Strong Acidic Electrolyzed Water with added Citron Juice, pH 2.57, ORP (oxidation-reduction potential) 1,122 mV, HClO 23.05ppm] and LAEW-CJ (Low Alkaline Electrolyzed Water with added Citron Juice, pH 4.67, ORP 997mV, HClO 42.55mV) as storing liquid for peeled taro. During storage at $5^{\circ}C$ until 30 days, SAEW-CJ and LAEW-CJ inhibited the growth of microorganisms more effectively than 0.2% (w/v) APS (aluminium potassium sulfate) and 0.85% (w/v) NaCl did. Total phenolic contents, PRO (polyphenol oxidase) activity, color differences value (${\Delta}E$) and vitamin C contents of peeled taro stored in SAEW-CJ and LAEW-CJ were lower than those stored in 0.2% APS and 0.85% NaCl. The hardness decrement of peeled taro stored in LAEW-CJ was lower than that of the others. In addition, the contents of moisture, crude protein, crude ash, total sugars, and reducing sugars were gradually decreased during storage. However, no difference by peeling methods or immersion liquid was found.

• Journal of Korean Society of Environmental Engineers
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• v.29 no.9
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• pp.1035-1043
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• 2007

A leachate containing an elevated concentration of organic and inorganic compounds has the potential to contaminate adjacent soils and groundwater as well as downgradient areas of the watershed. Moreover high-strength ammonium concentrations in leachate can be toxic to aquatic ecological systems as well as consuming dissolved oxygen, due to ammonium oxidation, and thereby causing eutrophication of the watershed. In response to these concerns landfill stabilization and leachate treatment are required to reduce contaminant loading sand minimize effects on the environment. Compared with other treatment technologies, leachate recirculation technology is most effective for the pre-treatment of leachate and the acceleration of waste stabilization processes in a landfill. However, leachate recirculation that accelerates the decomposition of readily degradable organic matter might also be generating high-strength ammonium in the leachate. Since most landfill leachate having high concentrations of nitrogen also contain insufficient quantities of the organic carbon required for complete denitrification, we combined a shortcut biological nitrogen removal (SBNR) technology in order to solve the problem associated with the inability to denitrify the oxidized ammonium due to the lack of carbon sources. The accumulation of nitrite was successfully achieved at a 0.8 ratio of $NO_2^{-}-N/NO_x-N$ in an on-site reactor of the sequencing batch reactor (SBR) type that had operated for six hours in an aeration phase. The $NO_x$-N ratio in leachate produced following SBR treatment was reduced in the landfill and the denitrification mechanism is implied sulfur-based autotrophic denitrification and/or heterotrophic denitrification. The combined leachate recirculation with SBNR proved an effective technology for landfill stabilization and nitrogen removal in leachate.

• Journal of the Korean Society for Marine Environment & Energy
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• v.4 no.4
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• pp.32-41
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• 2001

We measured the fluxes of COD, TN and 55 in addition to composition and quality of sediment in Komso Bay, West Coast of Korea. The fluxes of TN, SS and COD were measured during flood tide and ebb tide in April and August, 2000. Denitrification rates in the sediments was also measured by direct produced $N_2$ gas technique on April and August from 1999 to 2000 in the same sea area. The composition of the sediments were 0.33~5.67 % of sand, 20.2~25.6 of gravel and 68.7~77.0 % of silt. Ignition loss of the sediments were 6.58~7.50 %. The concentration of hydrogen sulfide in the sediments were 0.028~0.326 mg/gㆍdry and oxidation reduction potential of the sediments were -28~-15 mV Diurnal fluxes of COD, total nitrogen, and suspended solids with tidal current and denitrification rate in the tidal flat have been determined in Komso Bay The diurnal net flux of COD was same in April. While 14.4 ton COD/hr of net influx into the tidal flat was recorded in August. The diurnal net influx of total nitrogen was 0.16 ton N/hr in April and 1.13 ton N/hr in August. The diurnal net influx of suspended solids was 0.05 ton SS/hr in April, and also net influx of suspended solids was 0.29 ton SS/hr in August. The overall purification ability of the tidal flat were estimated 0.00~5.69 g COD/$m^2/day$, 0.06~0.45 g N/m²/day and 0.02~0.12 g SS/$m^2/day$ for COD, TN and SS, respectively. Denitrification rate was 0.009~1.720 m mole ${N_2}/m^2/day$ (average 0.702 m mole ${N_2}/m^2/day$) in April and 0.033~0.133 m mole ${N_2}/m^2/day$ (average 0.077 m mole ${N_2}/m^2/day$) in August, 1999. 0.000~l.909 m mole ${N_2}/m^2/day$ (average 0.756 m mole ${N_2}/m^2/day$) in April, 0.000~1.691 m mole ${N_2}/m^2/day$ (average 0.392 m mole ${N_2}/m^2/day$) in August, 2000. Even with a wide range of denitrification rate depending on the sampling location and studied periods, the average denitrification rate was estimated 0.482 m mole ${N_2}/m^2/day$ in the tidal flat of Komso Bay.

• Journal of Animal Science and Technology
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• v.52 no.5
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• pp.427-434
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• 2010

This study was conducted to investigate the growth performance and meat quality improvement according to castration, optimal feeding management and ruminally protected amino acid-enriched fatty acid (RPAAFA) for the unselected Hanwoo bulls in the performance test. Bulls were castrated at approximately 14 months of age. Sixteen Hanwoo steers, 15 months of age and weighing $412.9{\pm}24.9kg$, were distributed into 2 groups. Steers were fed a basal diet supplemented with RPAAFA at 0 g (control) or 100 g (treatment), respectively for 12 months. Steers were slaughtered at 27 months of age. Average daily gain for treatment tended to be higher (p=0.10) than that of control, whereas feed conversion ratio tended to be lower (p=0.07) in treatment than in control. The supplementation of RPAAFA did not affect rib eye area, back fat thickness, meat color, fat color, texture and maturity. The appearance rates of yield 'A' grade and high quality grade ($1^{++}$, $1^+$ and 1) were higher in treatment than in control. The content of moisture, fat, protein and ash in longissimus muscles were similar between control and treatment. The supplementation of RPAAFA did not affect water-holding capacity, oxidation and reduction potential, myoglobin and fatty acid contents in longissimus muscles. Thus, present results indicate that castration, optimal feeding management and RPAAFA may be recommended for improving growth performance and quality grade of the unselected Hanwoo bulls in the performance test.

• Korean Journal of Food Science and Technology
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• v.36 no.6
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• pp.937-942
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• 2004

Physicochemical properties and mineral composition of seaweed salts prepared by incineration and osmotic dehydration methods were determined. As the incineration temperature increased, yield of seaweed salts, insoluble solids, pH, alkalinity, and oxidation-reduction potential (ORP) decreased. Alkalinity of salt prepared with sea tangle was higher than that of sea mustard. ORP decreased by incineration above $700^{\circ}C$, and was lower in salt with sea tangle. As incineration temperature increased, amounts of K and Ca in seaweed salt increased, whereas that of Mg decreased. Potassium and Ca contents of seaweed salt increased remarkably compared with those of common salt. Potassium content of sea tangle salt was higher than that of sea mustard. As incineration time increased, yield of seaweed salts, insoluble solid content, and pH decreased, whereas ORP of the salt increased. Potassium content of seaweed salt with incineration time, while Ca and Na contents decreased after incineration of 8 and 4 hr, respectively. Yield of seaweed salt by osmotic dehydration increased as immersion time in sea water increased. pH of salt from sea mustard was higher than that of sea tangle. ORP of seaweed salt dried three times was -128.8 mV, significantly lower than that of salt prepared by incineration method. As sea water immersion time increased, Mg content of seaweed salt increased significantly, while Ca content decreased. Potassium content of seaweed salt was higher in sea tangle salt. In case of salt prepared by incineration of residuals, pH increased with immersion time but ORP decreased.