• The Journal of Korean Obstetrics and Gynecology
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• v.21 no.3
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• pp.34-59
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• 2008

Purpose: This study was performed to investigate the inhibiting effects of Gungguijohyeoleum on the development of experimentally-induced endometriosis in rats. Methods: Endometriosis was induced in rats by autotransplanting uterine tissue to the peritoneum and we divided them into three groups: (1) sham-operated group(n=8), (2) surgically induced endometriosis and untreated control group(n=8), (3) surgically induced endometriosis and Gungguijohyeoleum treated group(n=8), Gungguijohyeoleum was orally administrated for 15 days after operation. Then we measured the body weight, the volumes of endometriotic implants, the weight of uterus and ovary, and investigated the concentrations of cytokines(MCP-1, $TNF-{\alpha}$, $IL-1{\beta}$, IL-6) in peritoneal fluids. Histopathology, immunohistochemistry for COX-2 and VEGF, and histochemistry for mast cell in transplanted uterine tissue were performed. Results: - The volume ($mm^3$) of endometriotic implants in Gungguijohyeoleum treated group ($76.4{\pm}24.5$) was significantly decreased(p<0.05) compared with control group($222.1{\pm}109.1$). - The concentration (pg/ml) of MCP-1 in peritoneal fluids in Gungguijohyeoleum treated group ($1060.8{\pm}280.8$) was significantly decreased(p<0.05) compared with control group($1412.5{\pm}345.7$). - The concentration (pg/ml) of $TNF-{\alpha}$ in peritoneal fluids in Gungguijohyeoleum treated group ($827.5{\pm}219.9$) was significantly decreased(p<0.01) compared with control group($1126.2{\pm}139.9$). - The concentration (pg/ml) of $IL-1{\beta}$ in peritoneal fluids in Gungguijohyeoleum treated group($83.4{\pm}9.0$) was significantly decreased(p<0.01) compared with control group($105.3{\pm}17.6$). - The concentration (pg/ml) of IL-6 in peritoneal fluids in Gungguijohyeoleum treated group($108.9{\pm}15.7$) was decreased compared with control group($122.8{\pm}19.3$). - Histopathologically, proliferation of endometriotic epithelia, infiltration of inflammatory cells and angiogenesis in transplanted uterine tissue of Gungguijohyeoleum treated group were weakly observed than those of control group. - The percentage(%) of positive epithelial layers for COX-2 in Gungguijohyeoleum treated group($56.8{\pm}12.8$) was significantly decreased(p<0.01) compared with control group($75.1{\pm}16.3$). -The VEGF expression of endometriotic epithelia, neovascular endothelia and stromal cells in transplanted uterine tissue of Gungguijohyeoleum treated group was weakly observed than that of control group. - The numbers of mast cells in transplanted uterine tissue in Gungguijohyeoleum treated group($35.4{\pm}18.4$) were significantly decreased(p<0.01) compared with control group ($91.0{\pm}28.3$). Conclusion: On the basis of these results, we concluded that Gungguijohyeoleum has inhibiting effects on the development of transplanted uterine tissue. And these effects may be related with decreased production of MCP-1, $TNF-{\alpha}$, $IL-1{\beta}$, COX-2, VEGF and mast cells by administration of Gungguijohyeoleum.

• Journal of Life Science
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• v.19 no.5
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• pp.581-586
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• 2009

Bell pepper plants (Capsicum annuum cv. Plenty) were grown under low night temperatures (LNT: day/night temperature of $28/13^{\circ}C$) and optimum night temperatures (ONT: day/night temperature of $28/20^{\circ}C$) in growth chambers. Pollen grains were collected from plants in the growth chamber and incubated at 10, 15, 20, 25, and $30^{\circ}C$. After 24 hr incubation, in both ONT and LNT, the highest percent pollen germinations were observed at $25^{\circ}C$ followed by germinations at $30^{\circ}C$. Percent pollen germination at $25^{\circ}C$ was 42% in ONT - two times higher than in LNT at 21%. Pollen tube length was much longer at ONT than at LNT, regardless of incubation temperature. Compared with other treatments, earlier and quicker pollen tube elongation was observed in ONT pollen grains incubated at $25^{\circ}C$. To find pollen viability in plant growing conditions, pollen grains were incubated in LNT ($28/13^{\circ}C$) and ONT ($28/20^{\circ}C$) growth chambers for 24 hr. Petri-dishes with pollen grains were put in the growth chambers at the beginning of the night condition. Pollen grains in the LNT growth chamber did not germinate at night ($13^{\circ}C$), but began to germinate when the day condition ($28^{\circ}C$) started. Pollen grains in the ONT condition, however, started germinating from the early night ($20^{\circ}C$) and germination continued during the day ($28^{\circ}C$). Plants in LNT showed increased flower stalk length, ovary diameter, stamen length, flower weight, and fruit length. LNT conditions did not impair seed set. There were no differences in seed sets between fruits at LNT and ONT. Normal seed sets in LNT show that fertilization may be completed during daytime. However, further investigation is needed to find what extent of temperature stress causes malformed and/or parthenocarpic fruits in this bell pepper.

• Korean Journal of Animal Reproduction
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• v.23 no.2
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• pp.95-103
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• 1999

This experiment was conducted to investigate how the lactation regulation such as restricted-lactation and early weaning during the suckling period influences on ovarian functions and change in serum levels of progesterone in primiparous rats. All the rats were raised in the individual cage from a few days before parturition through the suckling period. The normal lactation(NL) groups were controled 8 pups. The restricted-lactation(RL) and weaned(W) groups were subdivided into 5 subgroups as RL0, RL5, RL10, RL15 and RL20 as well as W0, W5, W10, W15, and W20 according to the day of onset of suckling. The number of pups were regulated from 8 to 4 on experimental strating day in RL gropus, and also perfectly weaned on the each on-set day in W groups. The results obtained were summarized as follows: 1. During the whole suckling period of 25 days the pups in RL group grew significantly(P＜005) faster than those in normal-lactation(NL) group. The pups in earlier RL group grew significantly(P＜0.05) faster than those in later RL rats, and there was no found any significant difference in body weight of pups between RL20 and NL group. The gestation period and litter size were found to be 21.53$\pm$0.04 days and 13.75$\pm$0.07, respectively. 2. The estrous cycle was not expressed in the NL group through the whole suckling period. An irregular estrous cycle was found around day 20 in RL0 group, and the regular estrous cycles were exhibited continuously from day 10 in the day 0 weaned rats. 3. In the rats of NL group the serum progesterone concentration increased from 33.16$\pm$2.64ng/$m\ell$ on day 0 to 122.5$\pm$53.68 ng/$m\ell$ on day 10, and then decreased slightly to 97.30$\pm$3.21 ng/$m\ell$ on day 20, but then decreased abruptly. However, the serum level of progesterone decreased greatly(P＜0.05) in 5 to 10 days following suckling restriction in the rats from which suckling began to be under restriction on day 0 or day 15. In the early weaning group the significant ( (P＜0.05) decrease in progesterone concentration was found similarly in 48 hours following weaning in all the rats weaned on day 0 through day 20. It was suggested that lactation stimulation is a very pivotal on the function of ovary.

• Korean Journal of Ichthyology
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• v.19 no.1
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• pp.8-15
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• 2007

The morphology of gonad and reproductive cycle of ribbed gunnel (Dictyosoma burgeri) were investigated on the basis of histological observation. The specimens were monthly sampled in the coastal waters of Jeju from November 2001 to February 2003. The ovaries and testis of this species are categorized as cystovarian and lobule type, respectively. The gonadosomatic index (GSI) of female increased in November and maintained high values from December to February. The GSI of male was similar to that of female although it was decreased in February. The reproductive cycle can be grouped into the following successive stage in the ovary: growth (October to November), mature (November to February), spawning (January to February), and degenerating and recovery (March to September). And in the testis, the stage observed were: multiplication (August to November), growth (November to January), mature and spawning (November to February), and degenerating and recovery (January to September). The minimum maturation size of D. burgeri was over 15.0 cm and fecundity ranged from 2,194 to 6,581 eggs. The relationship between the fecundity and fish body was calculated in the fecundity (F) equation as: $F=0.4057TL^{3.1425}$ ($R^2=0.7621$) for total length (TL); $F=149.88BW^{0.9579}$ ($R^2=0.7982$) for body weight (BW), respectively. The fecundity was correlated positively with TL and BW. The histological observations of the gonads suggested that major spawning of this species probably occurs between January to February, when low water temperature ($13{\pm}0.3^{\circ}C$) period.

• Korean Journal of Poultry Science
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• v.35 no.1
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• pp.15-20
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• 2008

This study aimed at evaluating the effects of a molting diet method in molt induction and post-molt performance of laying hens. Eighty-one ISA Brown hens at 62 wk of age were randomly divided into three groups. After a 4-wk preliminary period, a control group was fed a corn-soybean-based layer diet, and for the other groups, molting was induced by starvation (MS) or feeding a molting diet (MD). For the MS group, feed was withdrawn for 2 wk; this was followed by feeding a layer diet every other day for 1 wk and then the control diet. The MD group was fed a molting diet containing low-protein and low-energy diet based for 4 wk; this was followed by feeding a layer diet. They had a free access to their diet and water. Egg production, egg quality, feed intake, and ovary and oviduct weights were measured throughout the experimental period. During molting, the feed intake in the MD group was lower than that of the control. Body weight of the molted groups was significantly reduced. The MS group feeding totally ceased egg production within 4d; after the initiation of feeding and decreased; in the MD group, egg production to 9.3% by d 10. On d 14, the ovaries and oviducts of the molted groups were distinctly lighter than those of the control. Throughout the post-molt period, egg production and egg shell thickness of the molted group improved; but there were no significant differences. Eggs from the MD-fed or control group were heavier than those of the MS-fed or control group. Finally, feeding of a low-protein and low-energy diet effectively induces molting and increase post-molt production, but further research will be conducted to determine the effects of the molt diet with other ingredients and to reduce the energy level of the molt diet for maximizing molt induction and post-molt egg quality.

• Journal of Food Hygiene and Safety
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• v.28 no.4
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• pp.360-366
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• 2013

The objective of this study is to investigate the effect of multivitamin on macrophage activity in Raw 264.7 cell and repeated oral dose toxicity in Sprague-Dawely rat of multivitamin. Raw 264.7 cells were treated with 50 and $100{\mu}g/mL$ multivitamin for 24 h. To measure the activity of macrophages, NO and TNF-${\alpha}$ assays were performed in Raw 264.7 cells. Treatment with 50 and $100{\mu}g/mL$ multivitamin for 24 h significantly increased production of NO and TNF-${\alpha}$ compared with control groups, indicating activation of macrophages. The female rats were treated with multivitamin of control group, low group (0.24 g/kg), medium group (1 g/kg) and high group (2 g/kg) intragastrically for 4 weeks, respectively. We examined the body weight, the feed intake, the clinical signs and serum biochemical analysis. We also observed the histopathological changes of liver, ovary, brain, adrenal gland, spleen, kidney, heart and lung in rats. No significant differences in body weights, feed intake, biochemical analysis and histopathological observations between control and multivitamin treatment group were found. In conclusion, multivitamin is physiologically safe and improve macrophage activity.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.6
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• pp.702-708
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• 2002

Although Umboniunm thomasi is one of marine mollusc (Archaeogastropoda: Trochidae) inhabiting the sands in the intertidal zone of the west coast of Korea, aspects of its reproductive biology are still not too well known. Reproductive cycle, gametogenesis, and first sexual maturity of U. thomasi collected at the west coast of Buan-gun, Jeollabuk-do, Korea were investigated monthly from January to December 1999. U. thomasi was dioecious, and an oviparous. The gonad was placed in the rear of the flesh part in the spiral shell. The external colors of the ripe ovary and testis appeared to be green and milk-white or yellowish white, respectively. Meat weigh rate peaked in July ($37.5\%$). And then the value sharply decreased in September ($28.3\%$), thereafter, gradually increased in November ($31.7\%$). Fully ripe oocytes were approximately 100$\~$110 $/mu$m in diameter, and their cytoplasm contained a great number of yolk Branules. Based on the monthly changes of the Bonadal development, gametogenesis, and meat weight rate, the reproductive cycle of U. thomasi could be devided into five successive stages: early active (November to April), late active (February to May), ripe (April to August), spawning (July to October), and recovery (September to February). Gonadal development and spawning were closely related to the seawater temperature, the main spawning occurred in September when the temperature reached above 24.2$^{\circ}C$. Individuals of 4.4 mm and less in shell height could not take part in reproduction in both sexes. Percentages of first sexual maturity of female and male shells ranging from 5.5 to 6.4 mm were $55.0\%$ and $61.9\%$, respectively, and $100\%$ of those over 7.5 mm in shell heights in both sexes participated in the reproduction.

• Neonatal Medicine
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• v.15 no.1
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• pp.80-83
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• 2008

Purpose : Inguinal hernias are common in children and sometimes are associated with dangerous complications, such as incarceration. There are no established management guidelines for ovarian hernias. We have reviewed the clinical course of ovarian hernias in infants. Methods : We reviewed the medical records of female infants diagnosed with ovarian hernias by ultrasonogram at Kwandong University College of Medicine, Cheil General Hospital, and the Women's Healthcare Center between March 2001 and August 2007. We analyzed the patients gestational age, birth weight, age at the time of detection of the inguinal mass, the patients chief complaints, operative time, post-operative complications, and ultrasonographic findings. Results : Eight female infants had ovarian hernias, four of whom were born prematurely. Seven infants had left-sided ovarian hernias, and one infant had a right-sided ovarian hernia. Five infants underwent surgery and there were no postoperative complications or recurrences. Three girls did not have surgery, and the ovarian hernias regressed spontaneously, with no recurrences or complications. The regression time of inguinal masses ranged from 70-161 days after birth. Conclusion : Physical examination to detect movable masses within the labium major in premature female infants is important because the incidence of premature inguinal hernias is much higher than in term infants. No rational medical treatment plans for female ovarian hernias have been published to date. We cared for three girls with spontaneous regression of ovarian hernias. Pediatricians should be aware whether emergent surgery for ovarian hernias is indicated.

• Korean Journal of Environment and Ecology
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• v.37 no.1
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• pp.86-93
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• 2023

This study investigated the ecological characteristics of Odontobutis interrupta at the Geumdang Stream from January to December 2021. The riverbed structure of the species habitat was rich in sand and mud. The water was deep, ranging from 21 to 124 cm, with an average of 48 cm. The stream velocity was slow at 0.24 (0.08-0.36) m/sec. The ratio of females to males was 1:0.98, and the total length of collected individuals ranged from 23 mm to 162 mm. The age according to the total length-frequency distribution as of May indicated that the group with a total length of 23-59 mm was one year old, the group with 60-99 mm was two years old, the group with 100-139 mm was three years old, and the group with 140-162 mm was four years old. As a secondary gender characteristic, the females genital papilla had a cylindrical shape, a hollow inside of the tip, and a longer diameter than males. The males had a cone shape with a pointed end. Sexually mature males had the nuptial color, with a black abdomen and whole body. Some females with a length ranging from 60 to 69 mm and all females 70 mm longer were sexually mature. Some males with a length ranging from 70 to 79 mm and all males 80 mm longer were sexually mature. The spawning season was from May to July, and the water temperature was between 19.6℃ to 29℃ during that period. The prosperous spawning season was June (26℃). The average number of eggs in the ovaries was 2,473 (883-4,955) per matured female, and the matured eggs were yellowish and spherical with a mean diameter of 1.42 (1.20-0.54) mm. The correlation between total length and weight was BW=0.0000006TL^3.21 with the constant a as 0.0000006 and parameter b as 3.21. The condition factor (K) was 1.67 (1.18-2.43) on average, and the slope was 0.116.

• Korean Journal of Environment and Ecology
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• v.38 no.2
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• pp.148-155
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• 2024

This study investigated the ecological characteristics of Odontobutis platycephala at Jaho stream from January to December 2022. The riverbed structure of the species' habitat was rich in cobble and pebble. The water was deep, ranging from 22 to 153 cm, with an average of 64 cm, and the stream velocity was rapid at 0.89 (0.42-1.46) m/sec. The ratio of females to males was 1:1.02, and the total length of collected individuals ranged from 38 to 156 mm. The age according to the total length frequency distribution as of May indicated that the group with a total length of 38-69 mm was one year old, the group with 60-99 mm was two years old, the group with 100-139 mm was three years old, and the group 140-156 mm was four years or older. As a secondary gender characteristic, the genital papilla was cylindrical in females and cone-shaped with a pointed tip in males. Some females with a length ranging from 60 to 69 mm and all females 70 mm or longer were sexually mature. Some males with a length ranging from 70 to 79 mm and all males 80 mm or longer were sexually mature. The spawning season was from May to July, and the water temperature was between 17 ℃ and 28 ℃ during that period. The prosperous spawning season was June (24 ℃). The average number of eggs in the ovaries was 988 (284-2,722) per mature female, and the mature eggs were yellowish and spherical with a mean diameter of 1.46 (1.19-1.71) mm. The correlation between total length and body weight is BW=0.00000006TL^3.12 with the constant a as 0.00000006 and the parameter b as 3.12. The mean condition factor (K) was 1.44 (0.96-2.26), and the slope was negative at -0.0007