A study has been made on phenomena of fertilization, hair genesis, and development of such forma of cotton flower as Mok Po 5, Red Leaf, Paymaster, Acala 1517w. 1) The pollen occurs to germinat at 4 hrs after its pollination and reaches the base of style 10 hrs. later, when 18 hrs elapsed, it gets enter at micropyle passes through placenta. 2) There may remain only one synergid as another one is occupied by pollen tube when it enters micropyle. 3) The fertilization completed at 18-48 hrs. after pollination. It was observed that Mok Po5 is the fastest, somewhat slower in Red Leaf and the slowest is the case of Paymaster in its speed. 4) Wool hair is formed by development of daughter cell which is. due to epidermal cell division' it starts its way right before blooming. 5) Wool hair is the most in its number covering about 60-80 within one hour ofter pollination and finishes its forming within 4-6 hours. The number is more abunbant at basal portion of ovule and rather at outer surface than at inner one of it. 6) The average length of wool hair checked at different time limit is as follows. 2.7-8.3$^{\mu} m$ 1 hr after pollination. 43.3-263.7$^{\mu} m$ 24-48 hrs. after pollination. The growth speed both of Mok Po 5 and Red Leaf get nearly same value but Paymaster is somewhat slower in its speed.
To investigate the morphological characteristics of spermatozoa of the grey red-blacked vole (Clethrionomys rufocanus) belongings to the subfamily Cricetinae, subgenus Clethrionomys were examined by scanning and transmission electron microscopes. The sperm head of C. rufocanus was an ax or hatchet in shape with a curved single dorsal hook. The total length of C. regulus sperm was 95.8 ${\mu}m$. The length of sperm head was 7.8 ${\mu}m$, and the tail (88.0 ${\mu}m$) consisted of four major segments: the neck (1.0 ${\mu}m$), middle piece (22.0 ${\mu}m$), and principal piece plus end piece (65.0 ${\mu}m$), respectively. The segmented columns were about 10~12 in number. The number of gyres of mitochondria ranged from about 170 to 178. The post-nuclear cap occupied about a half of nucleus. The equatorial segment is located between the post-nuclear cap segment and acrosomal cap on the nuclear surface. Nos. 1, 5 and 6 of the outer dense fibers were larger than the others. A fibrous sheath and longitudinal column of the principal piece were in evidence, but the fibrous sheath was not seen at the end piece. In conclusion, the morphological structures of sperm head and tail may be useful information to patterning of sperm evolution and classifying of species.
Consumers can choose suitable shoes for their feet in off-line shopping. However, in on-line shopping, because they can not wear shoes, compare to the off-line shopping, there are many problems in internet shopping. And the solution of user-oriented internet shopping is development of new type of prototype which is accessible to user and to offer visual information through 3D-virtual reality. We made this Prototype that the consumer can measure their own shoes size. And the consumer can print out their foot size in the internet database and measure their own foot size and type. And we maximized the visual experience though it is indirect and then we wanted to overcome a emotional experience in the real world. we visualized first, 'the softness' of shoes meterial, second 'the drainage' in the realtion of shoes meterial and water, third 'the close adhesion' in the realtion of shoes and consumer's their own foot and last 'the elasticity' in the relation of the shoes outer sole and surface. The result of this research can solve the problem in the existing on-line shoes' shop and it will become an alternative plan. And this prototype just will not become the localization at the on-line shoes' shop. In true sense, it will be an important example in the whole internet industry.
Marine benthic diatoms and environmental factors were studied at 60 sites on the Mangyung-Dongjin tidal flat of the west coast of Korea. Sediment samples were taken quantitatively from the upper 5 mm layer to obtain a representative estimate of the epipelic and epipsammic cell concentration. Surface sediments taken simultaneously with the quantitative diatom samples were analysed for the grain size. Exposure duration of study sites were calculated by the tide data recorded at Kunsan Outer-Harbour. Coarse sediments dominated mainly on the offshore coastal and lower tidal flat, whereas fine sediments occurred on inner and higher tidal flat. Total 371 diatom taxa were collected and the genera represented by a great number were Navicula and Nizschia. The 16 abundant species occupying more than 1% of total cell number are of the following: Paralia sulcata, Navicula sp. 1, Navicula arenaria, Cymatosira belgica, Amplora holsatica, Amphora coffeaeformis, Achnanthes hauckiana, Rhaphoneis amphiceros, Thalassionema nitzschioides. Navicula sp. 2, Dimeregramma minor, Amphora sp. 1, Cyclotella atomus, C, striata, Nitzschia kuetzingiana, Stephanodiscus sp. 1. The distribution pattern of these dominant species are described in relation to the habitat condition. Most of these species showed high densities in fine sediments. However, they occurred even silty sand and sandy sediments in low abundance. The epipsammic forms belonging to the Araphidineae and Monoraphidineae were restricted on the lower tidal flat. The typical species found in coarse sediments were: Cocconeis sp. 1, Opephora martyi, Amphora sabyii, Dimeregramma minor var. nana, Fragilaria virescens var. oblongella, F. virescens, Cocconeis grata. The higher tidal flat consisting of fine sediments showed relatively higher cell numbers than the lower tidal flat. River mouth region was the highest in abundance.
In November 2008, typical powdery mildew symptoms were observed on leaves of Arabidopsis thaliana ecotype Col-0 plants in a growth room under controlled laboratory conditions at Korea University, Seoul. The disease was characterized by the appearance of white powder-like fungal growth on the surface of infected leaves. As the disease progressed, infected leaves exhibited chlorotic or necrotic brown lesions, and leaf distortion and senescence. Conidiophores of the causal fungus were hyaline, unbranched, 3-4 celled, cylindrical, and $80-115{\times}6-9{\mu}m$ in size. Singly produced conidia (pseudoidium type) were hyaline, oblong to cylindrical or oval in shape, and $26-55{\times}15-20{\mu}m$ in size with a length/width ratio of average 3, angular/rectangular wrinkling of outer wall and no distinct fibrosin bodies. Appressoria on the hyphae were multi-lobed. These structures are typical of the powdery mildew Oidium subgenus Pseudoidium, anamorph of the genus Erysiphe. The measurements of the fungal structures coincided with those of Erysiphe cruciferarum. The phylogenetic analysis using ITS rDNA sequences revealed that the causal fungus Erysiphe sp. KUS-F23994 is identical to E. cruciferarum. The isolated fungus incited powdery mildew symptoms on the inoculated Arabidopsis leaves, which proved Koch's postulates. Taken all data together, we first report the occurrence of powdery mildew disease of A. thaliana caused by Erysiphe cruciferarum in Korea.
It has been demonstrated that intracoronal bleaching of pulpless teeth may result in cervical root resorption. Several authors postulated that bleaching agents such as hydrogen peroxide penetrated through the dentinal tubules to damage the surrounding tissues that cause cervical root resorption. The purpose of this study was to suggest on in vitro model for direct determination of hydrogen peroxide penetration through CEJ during nonvital bleaching. In addition, this model permit the quantification of the amount of hydrogen peroxide penetrated during the procedure. Freshly extracted intact premolars, removed for orthodontic reasons were used. Root canal treatment was performed in each tooth. And then the outer surface and crown portion of the teeth was sealed with wax leaving the CEJ. The prepared teeth mounted on the wax laminates were placed in plastic assay tubes containing 1.5ml bidistilled water with their entire root, including the CEJ, submerged in the solution. The teeth were dividied into four groups. Thermo group : thermocatalytic bleaching with superoxol Walk group: walking bleaching with sodium perborate & superoxol Combi group : combination of thermocatalytic & walking bleaching Dw group : walking bleaching with sodium perborate & water The bleaching procedure was performed three times. The bleaching intervals were at 3 days. The hydrogen peroxide present in the assay system was added to ferrous ammonium sulfate resulting in ferric ion release. Upon the addition of potassium thiocyanate a ferrithiocyanate complex results, which absorbs light at the wavelength of 467nm. The radicular penetration of hydrogen peroxide in the four groups was assessed directly using spectrophotometer. The amount of hydrogen peroxide in the samples tested is determined by comparing them with a standard curve generated by known amounts of hydrogen peroxide. The results were obtained as follows : 1. In all experimental groups except the Dw group showed lower penetration amount in day 4 than day 1, there was statistical importance in the difference (P<0.05). 2. After 3rd treatment, Thermo group showed slightly increased value and narrow distribution. Walk group showed much more penetration amount and widely dispersed value. Value of Combi group showed wide distribution without regard to treatment time, but value of Dw group evenly distributed. 3. Thermo group, Walk group and Dw group showed a tendency of increasing penetration amount with increasing treatment times(P<0.01), but Combi group revealed no statistically important differences. 4. Combi group showed the highest degree of penetration. Walk group showed lower penetration than Combi group. Thermo group & Dw group showed lower than Walk group. 5. Cervical root permeability to hydrogen peroxide varied from 0 to 35 %.
Involvement of the facial nerve(herpes zoster oticus, Ramsay Hunt Syndrome) is a rather common clinical syndrome. It begins with unilateral ear pain, followed shortly by a peripheral facial palsy. Paresis or paralysis may affect the muscles of facial expression, which also close the eyelids. The levator palpebrae which is innervated by the 5th cranial nerve is spared, so the eye may remain open. The rash is usually confined to the tympanic membrane and the external auditory canal. It may spread to involve the outer surface of the lobe of the ear, anterior pillar or the fauces and mastoid. There also may be a loss of taste in the anterior two thirds of tongue. At time, the auditory nerve involvement produces tinnitus, deafness and vertigo. The 5th, 8th and 10th nerves and even the upper cervical spinal nerve can be involved presumedly on the base of spread of the infective process along anastomotic connections between the facial nerve. The facial paralysis is identical to that of Bells palsy. Frequently the recovery of facial nerve function is incomplete, leaving the patient with some residual facial weak ness. We experienced 2 cases of Ramsay Hunt Syndrome. The first patients, 55 year old male, visited our pain clinic on the day when his left facial nerve start to paralyze. We injected 6 ml of 0.25% bupivacaine into his left stellate ganglion 15 times. TENS was also applicated simultaneously. His facial paralysis was recovered completely 3 weeks after treatment without any complications. Another one, 53 year old male, visited us 7 weeks after onset of facial paralysis. He has been treated conventional oriental method(acupuncture, massage, warm application, etc). But the degree of his left facial paralysis didn't improve at all He has been treating with SGB 50 times and TENS for 2 months. Temporal and zygomatic branch of his left facial nerve recovered nearly completely but buccal and mandibular branch did not recover completely. We are willing to insist on the early treatment is the best choice in managing of Ramsay Hunt Syndrome.
The distributions of the temperature and salinity in Kamak bay which has two channels and three sea bottom topographic parts were studied by taking the detailed hydrographic data at the ebb and flood during four seasons from May, 1988 to Feb., 1989. The general pattern of the distributions of characteristics which the temperature and salinity has in Kamak bay is basically formed by the topography and sea water movement of the bay. The changes of these distributions by seasons mainly come from the heating and cooling of the sea surface and the increase of the run-off. The bay has three remarkable water masses and the their general characteristics are follows: the inner bay water has a stagnation character influenced by the inland and the concave of the sea bottom in the north west, Yosu harbor water has an estuary character of the low salinity caused by the run-off of Somjin river and Yon Tung brooklet in the north east, and the outer bay water has an out-sea character, as it is located near by the big mouth in the south of the bay. The distributions of those water masses at the ebb and flood show some different features due to the flow patterns, and the daily changes of oceanic conditions at the vicinity of Hangdae-ri are so big that it may influence the habitation and production of the living things in the bay.
Gonad structure, germ cell development and reproductive cycle of the smallmouth scorpionfish, Scorpaena miostoma were investigated based on histological method. Samples were collected monthly in the vicinity of Suyoung Bay, Pusan, Korea from November 1995 to October 1996. The testis is seminiferous tubule type in internal structure. Seminiferous tubule consists of numerous testicular cysts which contain numerous germ cells in same developmental stage. The ovary consists of several ovarian lamellae originated from ovarian outer membrane. Oogonia originated from the inner surface of the ovarian lamella protrude to the ovarian cavity in oocyte stage, and they are suspended by the egg stalk. Biological minimum size of female and male were 12.5cm in total length. Gonadosomatic index (GSI) of female (3.81) and male (0.23) were the highest in October. Reproductive cycle was classified into the following successive stages: in female, growing stage $(May\~August)$, maturation stage $(September\~October)$, ripe and spawning stage $(November\~December)$, recovery and resting stage $(January\~April)$, and in male, growing stage $(June\~August)$, maturation stage $(September\~October)$, ripe and spent stage $(November\~January)$ and recovery and resting stage $(February\~May)$.
Jeon, Boo Seong;Nam, Seung Won;Kim, Sunju;Park, Myung Gil
ALGAE
/
v.33
no.1
/
pp.1-19
/
2018
Members of the family Parviluciferaceae (Alveolata, Perkinsozoa) are the well-known dinoflagellate parasitoids along with Amoebophrya ceratii species complex and parasitic chytrid Dinomyces arenysensis and contain six species across three genera (i.e., Parvilucifera infectans, P. sinerae, P. rostrata, and P. corolla, Dinovorax pyriformis, and Snorkelia prorocentri) so far. Among Parvilucifera species, the two species, P. infectans and P. sinerae, are very similar or almost identical each other morphologically and genetically, thereby make it difficult to distinguish between the two. The only main difference between the two species known so far is the number of sporangium wall (i.e., 2 layers in P. infectans vs. 3 layers in P. sinerae). During sampling in Masan bay, Korea during the spring season of 2015, the dinoflagellate Akashiwo sanguinea cells infected by the parasite Parvilucifera were observed and this host-parasite system was established in culture. Using this culture, its morphological and ultrastructural features with special emphasis on the variation in the number of sporangium wall over developmental times, were investigated. In addition, the sequences of rDNA regions and ${\beta}-tubulin$ genes were determined. The result clearly demonstrated that the trophocyte at 36 h was covered with 4 layers, and then outer layer of the sporocyte gradually degraded over time, resulting in wall structure consisting of two layers, with even processes being detached from 7-day-old sporangium with smooth surface, indicating that the difference in the number of layers seems not to be an appropriate ultrastructural character for distinguishing P. infectans and P. sinerae. While pairwise comparison of the large subunit rDNA sequences showed 100% identity among P. infectans / P. sinerae species complex, genetic differences were found in the small subunit (SSU) rDNA sequences but the differences were relatively small (11-13 nucleotides) compared with those (190-272 nucleotides) found among the rest of Parvilucifera species (P. rostrata and P. corolla). Those small differences in SSU rDNA sequences of P. infectans / P. sinerae species complex may reflect the variations within inter- strains of the same species from different geographical areas. Taken together, all morphological, ultrastructural, and molecular data from the present study suggest that they are the same species.
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