• Analytical Science and Technology
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• v.22 no.4
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• pp.277-284
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• 2009

The germination rate of radishes grown with the far infrared irradiated water and drinking water was 100% and 78% respectively. The far infrared irradiated water stimulated the cell division of the cotyledons and enlarged the cell sizes both in the dark and in the light. In the dark and light conditions, the size of the cotyledons of transversal axis and longitudinal axis grown with the far infrared irradiated water was bigger than that grown with the drinking water. The content of chlorophyll and the consumption of $CO_2$ of the cotyledons grown with the far infrared irradiated water were higher, respectively. Osmotic pressure of the cotyledons grown with the far infrared irradiated water was 1.25 factors higher than that grown with the drinking water. The water potential of the cotyledons grown with the far infrared irradiated water was more negative value. The length of hypocotyls grown with the far infrared irradiated water was 2.18 factors longer in the dark, 1.99 factors longer in the light than that grown with the drinking water and the radish roots grown with the far infrared irradiated water were larger, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.5
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• pp.534-538
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• 1997

Some physiological characteristics and cultivar differences of winter barley to salt stress were studied during seedling stage. Salt stress was caused by adding NaCl solution to the pot culture soil. Measurements of the responses to salt stress and of the responses after relief from stress were done in terms of leaf water potential, chlorophyll and free proline contents, seedling height and seedling dry weight, and survival rate of leaves. Under salt stress ($\Psi_{\pi}$ =-20bar) seedling height and seedling weight were decreased by 2~22% and by 25~39% respectively, showing some differences among cultivars. Chlorophyll contents was decreased by 33~49%, and free proline content was remarkably increased from control 0.2~0.3mg to salt stress 9.6~14.7mg. The leaf water potential of seedling grown under salt stress with NaCl solution($\Psi_{\pi}$ =-10 or -20bar) was decreased from control -3.3bar to salt stress -9.0bar or -16.2bar respectively but there were no large differences among cultivars with time after relief from salt stress. Leaf survival rate was high in order of Baegdong, Milyang12, Olbori, Durubori and Hyangmaeg, and decrease rate of seedling dry weight was low in the order of Baegdong, Olbori, Hyangmaeg, Milyang12, Durubori. The increase in free proline contents was high in the order of Milyang12, Hyangmaeg, Baegdong, Durubori and Olbori.

• Journal of Korean Society of Forest Science
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• v.101 no.3
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• pp.461-468
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• 2012

This study was conducted to study the seasonal changes of relative water relations parameters by pressure-volume curves of Korean mistletoe (Viscum album var. coloratum) leaves for understanding ecological characteristics. In growing season, the osmotic potentials at full turgor (${\Psi}_o^{sat}$) and at incipient plasmolysis (${\Psi}_o^{tlp}$) decreased, while increased the maximum bulk elastic modulus of the cell wall ($E_{max}$) and relavive water content ($RWC^{tlp}$). Korean mistletoe in Quercus variabilis and Korean mistletoe in Quercus mongolica in November showed best maximum perssure potential (${\Psi}_{P,\;max}$). Pressure potential (${\Psi}_P$) and water potential (${\Psi}_L$) in Korean mistletoe in Quercus variabilis were rapidly decreased with decreasing of relative water content. The values of $RWC^{tlp}$ in November were all above 84% showing that the function of osmoregulation is somewhat better, and symplastic water content (Vo/DW) and maximum water content (Vt/DW) were variable seasonally. Thus, responses to water relations of Korean mistletoe in Quercus variabilis and Korean mistletoe in Quercus mongolica such as ${\Psi}_o^{sat},\;{\Psi}_o^{tlp},\;E_{max},\;{\Psi}_{P,\;max},\;RWC^{tlp}$ showed increaing drought tolerance with increasing of leaf aging.

• Korean Journal of Plant Resources
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• v.24 no.4
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• pp.413-418
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• 2011

This study was carried out to establish a proper cultivation site and diagnose the drought-tolerance of Aster scaber and Synurus deltoides leaves by using Pressure-volume curves. In order to measure pressure-volume (P-V) curves, Aster scaber and Synurus deltoides were cut off above ground part and the tip of the cutting were placed in water, which was covered with a plastic bag. Samples were kept overnight (about 12 hours) in darkness at room temperature (20~25$^{\circ}C$) to achieve maximal turgor (full saturation). The pressure in the chamber was gradually increased from 0.3MPa to 1.8MPa by nitrogen gas. After measured, leaf samples were dried at 80$^{\circ}C$ for 48 hours and dry weight of each samples were determined. The result of the original bulk osmotic potential at maximum turgor ${\Psi}^{sat}_o$ sat was lower -0.8 MPa in Aster scaber leaves than -0.7 MPa Synurus deltoides leaves. Also the osmotic potential at incipient plasmolysis ${\Psi}^{tlp}_o$ in Aster scaber leave was -0.9 MPa. In contrast, the value of maximum bulk modulus of elasticity $E_{max}$ of Aster scaber leaves were approximately two folds higher than that of Synurus deltoides leaves. The values of the relative water content at incipient plasmolysis $RWC^{tlp}$ are all above 90% showing that the function of osmoregulation is somewhat better, and Vo/DW, Vt/DW, Ns/DW of Synurus deltoides leaves were approximately 1~2 times higher than that of Aster scaber leaves. Thus, responses to water relations of Aster scaber and Synurus deltoides such as ${\Psi}^{sat}_o$, ${\Psi}^{tlp}_o$, $E_{max}$, ${\Psi}_{P,max}$, $RWC^{tl}$ were shown that the Aster scaber leaves was slightly higher drought-tolerance than Synurus deltoides leaves. However, in both of Aster scaber and Synurus deltoides, occurring incipient plasmolysis at the high water content, have a relatively lower drought-tolerance property indicating that growth of these plants are cultivated appropriate in high moisture soil sites.

• Korean Journal of Environment and Ecology
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• v.30 no.1
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• pp.130-134
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• 2016

How does $CO_2$ affect on the stomatal mechanism? The mechanism of stomatal opening by $CO_2$ is not clear as it is difficult to see $CO_2$ effect on light-induced stomatal opening. Furthermore, stomata may react differently according to the concentration of $CO_2$. The significance of the possible endogenous rhythms must consider to understand on $CO_2$-related response. It is clear that $CO_2$ has an effect on the accumulation of osmotic materials which determines the degree of stomatal apertures because it is known that stomata open in the condition of the reduced $CO_2$ concentration. However, it is not fully understood how $CO_2$ leads to the stomatal opening. It has been thought that $CO_2$ can not affect on the ion fluxes which determines the increase of osmotic potential in guard cells. However, in this study, the changes of guard cell membrane permeability by $CO_2$ have been focused on. There are many reports that $CO_2$ related reactions are dominant when the leaf is exposed to certain a mount of $CO_2$. The hypothesis of the stomatal opening by light is based on the increase of osmotic materials in guard cells including $K^+$, $Cl^-$, sucrose and $malate^{2-}$. It was reported that $CO_2$ induced a big hyperpolarization indicating that $H^+$ was extruded to the cell outside. It was also found that $CO_2$ caused guard cell membrane hyperpolarization in the intact leaf up to 3 or 4 times higher than that of light induced membrane hyperpolarization. These results represent that $CO_2$ can affect on the change of physical characteristics which affects on the change of the membrane permeability.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.2
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• pp.91-96
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• 2004

Accumulation of nitrate in edible crops is undesirable due to potential risks to human health. Since nitrate has a role in the osmotic regulation of plants, salt accumulation in soil is expected to stimulate nitrate accumulation in plants. Lettuce (Lactuca sativa L.) was grown in soils of different salinities, 9.69 and $4.49dS\;m^{-1}$, in a greenhouse, and the effect of soil salinity on nitrate accumulation in lettuce was investigated. Content of nitrate in the lettuce increased significantly as soil salinity increased under low light intensity and ample supply of nitrate in root media. Soluble sugar and oxalate contents in lettuce were also significantly higher in the soil of higher salinity. Phosphate, Cl, and $SO_4$ contents in lettuce were not significantly different in soils of different salinities. Among the cations, K content in lettuce was significantly higher in the soil of higher salinity, but Na, Ca, and Mg comtents were not much influenced. Comparing to the lettuce grown in low salinity soil, although the growth of lettuce was decreased by 9% in the soil of higher salinity, nitrate accumulation in the lettuce was increased by 18.6%. These results indicate that higher nitrate content in lettuce of higher salinity soil is a positive accumulation to adapt to the water stress condition. The nitrate accumulation of vegetables grown in plastic film houses is known to be due to the heavy fertilization and low light intensity, but salt accumulation in the soil, which can lower soil water potential, is expected to stimulate the nitrate accumulation further.

• Journal of Ecology and Environment
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• v.35 no.4
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• pp.351-358
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• 2012

To investigate the environmental adaptation and ecophysiological characteristics of Suaeda maritima and S. asparagoides under saline conditions, plant growth and density were analyzed according to environmental changes of habitats. The total ion content of soil decreased with time, which was caused by the predominance of exchangeable $Na^+$ and $Cl^-$ in the upper layers. The population of S. maritima was more densely distributed in the region with higher ion contents of $Cl^-$, $Mg^{2+}$, $K^+$ and $Na^+$ than the population of S. asparagoides. Both species were showed a decreased population density according to increases in plant growth. Under the conditions of a salt field, S. maritima and S. asparagoides contained high inorganic ions to maintain low water potential, but low water soluble carbohydrate contents. In the case of free amino acid, S. maritima showed an especially high proline content, and contained rather large amounts of free amino acids, whereas S. asparagoides did not. Both species showed high inorganic ion contents in the leaves, which might be a mechanism of avoiding the ionic toxicity by diluting the accumulated ionic concentration with a high ratio of water content to dry weight. This result suggests that S. maritima seems to adapt to saline conditions by accumulating proline in addition to inorganic ions. S. asparagoides seems to adapt by osmoregulation processes, using inorganic ions rather than free amino acids.

• The Plant Pathology Journal
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• v.27 no.3
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• pp.266-271
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• 2011

Physicochemical characteristics and heavy metal content of soils located along the drainage way of an abandoned mine at Busan, Korea ($35^{\circ}31'N$, $129^{\circ}22'E$) (contaminated soil; CS) and uncontaminated soils (50-70 m apart from the drainage way) (NS) were examined. Growth of tomato plants (Solanum lycopersicom cv. Rutgers) in CS and NS, development of the root-knot nematode (Meloidogyne incognita) as root-knot gall formation on tomato plants, and non-parasitic nematode populations in soil were also examined. Growth of tomato plants, root-knot gall formation, and non-parasitic nematode populations were significantly reduced in CS with higher As content, lower pH, higher electrical conductivity (EC), and lower available phosphate (av. $P_2O_5$) than in NS. None of the other physicochemical characters examined differed significantly between CS and NS (low and no significance) and were above or below the critical levels detrimental to plant growth and nematode development, suggesting that As may be the primary hazardous heavy metal in CS. The toxicity of As might be enhanced at low pH in CS because exchangeable forms of some heavy metals increase with the decrease of soil pH. The heavy metals, especially As, may have contributed to increasing EC and decreasing av. $P_2O_5$. Therefore, the effects of mine drainage contamination from the abandoned mine were derived primarily from contamination by heavy metals such as As. These may have been enhanced in toxicity (solubility) by the lowered pH, increased soil salinity (EC) and decreased av. $P_2O_5$. Our results suggest synergistic adverse effects on the plant and the nematode by decreasing osmotic potential and nutrient availability.

• Korean Journal of Environmental Agriculture
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• v.17 no.4
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• pp.358-361
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• 1998

This study was carried out to develop a short term hardening method of tree seedlings of Rhus chinensis Mill., Evodia daniellii Hemsley and Parthenocissus tricuspidata(Sieb. et Zucc.) Planck for afforestation on a concave and a crack of cut-rock slope. The seedlings were grown in a cylinder shaped pot made of polyvinyl net with the soil media of peatmoss, vermiculite, clay, compost, fertilizer, and absorbant(40:25:19:15:1:0.1, v:v). They were cultivated in a greenhouse for four months and in field condition for two months. During the last three months of the growing period the seedlings were hardened by periodic desiccation and irrigation in 4 to 10 days interval. The hardened seedlings showed lower leaf water potential, higher leaf osmotic pressure, and lower T/R ratio than those before the hardening. The hardened seedlings survived well on the soil medium in the concave of cut-rock slope.

• Journal of Bio-Environment Control
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• v.6 no.4
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• pp.292-298
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• 1997

These studies were conducted to verify the effect of the supplementary lighting by reflective film mulching and its establishment in the north side of greenhouse on the utility of light at tomato by means of investigating changes of leaf temperatures, stomatal features, transpiration rates and photosynthetic rates. Stomatal density of leaves were high in the reflective film mulching but sizes of stomata were not different. As the osmotic potential in rooting zone was low, the stomatal resistance was high, transpiration rate was low, and leaf temperature was increased by 40.62$^{\circ}C$. And also in the block of reflective film mulching photosynthetic rates were decreased hut chlorophyll contents were not different. Especially, there is an effect of controlling greenhouse whiteflies by treatment of reflective film mulching. It is thought that the reason of high quality or increasing yield at several crops by supplementary lighting, such as reflective film mulching, would be caused by influences of absorption and distribution of nutrients through high transpiration rate and photosynthesis which resulted from increase of stomata.