• Title/Summary/Keyword: OsPT

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Expression of OsPTs-OX Transgenic Rice in Phosphate-Deficient Condition (무인산 조건에서 OsPTs 유전자 도입 형질전환벼의 인산흡수 반응)

  • Song, Song-Yi;Yi, Gi-Hwan;Park, Dong-Soo;Seo, Jong-Ho;Son, Beom-Young;Kim, Do-Hoon;Nam, Min-Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.3
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    • pp.264-272
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    • 2011
  • It needs to develop high phosphate-available rice that is able to minimize environmental pollution caused by phosphate fertilizer. Then we have transformed 4 rice transporter genes, OsPT(Oryza sativa Phosphate Transporter)1, OsPT4, OsPT7 or OsPT8, to rice (Oryza sativa cv. Dongjin) via Agrobacterium-mediated transformation. We tested adaptation in the P-deficient condition of Dongjin (parental) and each transgenic line in the pot and the field conditions. Definite physiological changes have been observed in OsPTs transgenic lines including culm length, root formation and heading date. Phosphate uptake at harvesting stage was about three times higher in OsPT1-OX (overexpression) and OsPT4-OX than in Dongjin (wt) without P application. There are no variations in total phosphate-content of brown rice of OsPT1-OX in spite of high phosphate uptake. Practically the expression of OsPT1 has contributed to stabilize grain production without P fertilization in rice cultivation than Dongjin.

The Effects of 2nd Metals in Pt-based Electrocatalysts on Methanol Oxidation (Pt합금 촉매에서 메탄을 산화 반응에 미치는 제2금속의 영향)

  • Kim Yeong Min;Park Gyeong Won;Choi Jong Ho;Park In Su;Seong Yeong Eun
    • 한국전기화학회:학술대회논문집
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    • 2002.07a
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    • pp.179-182
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    • 2002
  • The electrooxidation of methanol was studied using Pt, PtRu(1:1), PtNi(1:1), PtRh(1:1) and PtOs(1:1) alloy nanoparticles for application as electrocatalysts. The effects of the second metals in the electrocatalytic activity was investigated using cyclic voltammetry (CV), chronoamperometry (CA), X-ray photoelectron spectroscopy (XPS). There are the metallic and oxygen states in the PtRu and PtOs electrocatalysts . In the XPS of PtRu and PtOs alloy nanoparticles, the oxygen sources were dominant as the second metal's effects. Negative shifts of the binding energies of Pt for the PtNi, PtRh alloy nanoparticles were determined by XPS measurements, which can be explained by electronic effects.

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Tetrathiafulvalene (TTF) Charge Transfer Compounds with Some Heavier Transition Metal (Au, Pt, Ir, Os) Chlorides

  • 정찬규;김영인;최성낙
    • Bulletin of the Korean Chemical Society
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    • v.17 no.11
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    • pp.1061-1065
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    • 1996
  • The charge transfer compounds of tetrathiafulvalene (TTF) with the general formula of (TTF)mMCln, (M=Au, Pt, Ir, Os) were prepared by the direct reaction using excess HAuCl4·3H2O, H2PtCl6·xH2O, H2IrCl6·xH2O and H2OsCl6 respectively. The powdered electrical conductivities (σrt) at room temperature are given as follows; (TTF)3AuCl2, 4.53×10-3; (TTF)3.5AuCl2, 6.37×10-3; (TTF)3PtCl4, 5.51×10-4; (TTF)2IrCl4, 2.40×10-5; (TTF)OsCl4·1/2C2H5OH, 4.46×10-7 Scm-1. Magnetic susceptibility, electronic (UV-Vis.), vibrational (IR) and EPR spectroscopic evidences indicate that there is incomplete charge transfer from the TTF donor to gold, platinum, and iridium respectively, and that there is essentially complete charge transfer to osmium, thereby resulting a relatively low electrical conductivity in osmium compound. The EPR and magnetic susceptibility data reflect that the metals are in diamagnetic Au(Ⅰ), Pt(Ⅱ), Ir(Ⅲ), and Os(Ⅱ) oxidation states, and the odd electrons are extensively delocalized over the TTF lattices in each compound.

Functional Analysis of the High Affinity Phosphate Transporter Genes Derived from Oryza sativa in Arabidopsis thaliana. (애기장대에서의 벼 유래의 고친화성 인산 운반체 유전자들의 기능 분석)

  • Seo, Hyoun-Mi;Jung, Yun-Hui;Kim, Yun-Hye;Kwon, Tack-Min;Jeong, Soon-Jae;Yi, Young-Byung;Kim, Doh-Hoon;Nam, Jae-Sung
    • Journal of Life Science
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    • v.18 no.4
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    • pp.488-493
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    • 2008
  • Phosphate, a favorable phosphorous form for plant, is one of major nutrient elements for growth and development in plants. Plants exhibit various physiological and biochemical responses in reaction to phosphate starvation in order to maintain phosphate homeostasis. Of them, expression of high affinity phosphate transporter gene family and efficient uptake of phosphate via them is a major physiological process for adaption to phosphate deficient environment. Although the various genetic resources of high affinity phosphate transporter are identified recently, little is known about their functions in plant that is prerequisite information before applying to crop plants to generate valuable transgenic plants. We demonstrated that Arabidopsis transgenic plants over-expressing two different high affinity phosphate transporter gens, OsPT1 and OsPT7, derived from rice, exhibit better growth responses compared with wild-type under phosphate starvation condition. Specially, OsPT7 gene has proven to be more effective to generate Arabidopsis transgenic plant tolerant to phosphate deficiency than OsPT1. Furthermore, the expression level of AtPT1 gene that is one of reporter genes specifically induced by phosphate starvation was significantly low compared with wild-type during phosphate starvation. Taken together, these results collectively suggest that over expression of OsPTl and OsPT7 genes derived from monocotyledonous plant function efficiently in the dicotyledonous plant, relieving stress response caused by phosphate starvation and leading to better growth rate.

A Study on the Anxiety of Open Heart Surgery Patient's Family (개심술 환자 가족의 불안에 관한 연구)

  • Choi, Young-Hee
    • The Korean Nurse
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    • v.31 no.5
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    • pp.29-38
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    • 1992
  • The purpose of this is to find the anxiety score of OHS pt's family and general surgery pt's family and to offer basic data. The subjects of this study were 80 : 40 were OHS pt's family in Seoul Hospital and remaining 40 were OS pt's family in Capital Hospital. The data was collected between March 1, through May 30, 1992. The instruments used for this study were Zung's The Self-Rating Anxiety Scale. The collected daLa were analyzed using percentage, descriptive statistics, $X^2-test$, t-test and Two-way Anova. The finding's were as followed: 1. Test of hypothesis "The anxiety score of the Ol-lS pt's family were similar to as pt's family" was rejected (L=0.772 P>.05) 2. The mean score of anxiety of the OHS pt's family was 1.772, The mean score of anxiety of the OS pt's family was 1,790. 3. The relationship between anxiety of the OHS pt's family and GS pt's family and family's age was significant (P<.Ol) and the relationship between anxiety and age interaction effect. (F=242.0 P<.01) In conclusion, the anxiety score of the OI-IS pt's finding and GS pt's family was all high.

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Synthesis and Characterization of Octamethylenethiafulvalene Compounds with Osmium, Iridium, Platinium and Gold Chloride (Octamethylenethiafulvalene과 염화오스뮴, 이리듐, 백금 및 금 화합물의 합성과 특성에 관한 연구)

  • Jeong, Chan Kyou;Lee, Hong Woo;Kim, Young Jin;Choi, Sung Nak;Kim, Young Inn
    • Journal of the Korean Chemical Society
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    • v.45 no.5
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    • pp.442-447
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    • 2001
  • The charge-transfer compound (OMTTF)AuCl$_4$ was prepared from the direct reaction of octamethylenethiafulvalene (OMTTF) with HAuCl$_4{\cdot}xH_2$O in THF. (OMTTF)$_2PtCl_4$, (OMTTF)_2IrCl_6{\cdot}2H_2$O, and (OMTTF)Os$Cl_5{\cdot}THF$ were also formed using $H_2PtCl_6{\cdot}xH_2O$, $H_2IrCl_6{\cdot}xH_2O$ and $H_2OsCl_6$, respectively. The prepared compounds were characterized by magnetic (EPR, magnetic susceptibility), spectroscopic (IR, UV-Vis), electrochemical (CV) methods, and the powdered electrical conductivity measurement. The powdered electrical conductivities at room temperature were ~$10^{-7}S{\cdot}cm^{-1}$. The experimental results show that $OMTTF^+$ monocation radicals exist in all of the prepared compounds. The redox potential of OMTTF supports that $OMTTF^+$ is relatively stable. The magnetic properties indicate that there are significant magnetic interactions between the localized odd electrons on the central metal (Ir and Os) ions and the odd electrons resided on $OMTTF^+$ cation radicals in both (OMTTF) $_2IrCl_6{\cdot}2H_2O$ and (OMTTF)$OsCl_5{\cdot}THF$.

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Temperature Effect on the Growth Parameters of Rice during Vegetative Period

  • Yin Myat Myat Min;Seo-Young Yang;Hyeon-Seok Lee;Myeong-Gu Choi;Chung-Gun Lee;Woon-Ha Hwang
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.133-133
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    • 2022
  • Temperature is a crucial environmental factor for rice cultivation due to the climate change and can influence the rice growth and development. Therefore, the effect of temperature on plant growth characters was examined during the vegetative stage. Plants were grown under three different temperatures: 23℃/13℃ for 18℃, 26℃/16℃ for 21℃ and 29℃/19℃ for 24℃ in the phytotron. The temperature was treated after transplanting and ended in early panicle initiation stage. Heading date of the two varieties were strongly affected by the temperature and were delayed in the 18℃. The plant height in the 18℃ was 21 % shorter than the 21℃ and 24℃ and the tiller and leaf number were increased in the 18℃. All the growth rates of the characters were the slowest in 18℃. The stem dry weight was significantly increased in 18℃. Nitrogen content was increased in the leaves of 18℃ whereas available phosphate and potassium content was found to be increased in the stems of 21℃ and 24℃. OsNRT 2.1 was overexpressed in the leaves and stems of 18℃ and OsNRT2.3a could be expressed in 18℃ and 21℃ temperatures whereas more expressed in 21℃. OsPT1 and OsPT6 could be expressed in the leaf of 18℃ and 24℃ but could be expressed in the stem of 18℃. OsHAK1 and OsHAK5 could be overexpressed in the leaves and stems of 18℃. For hormone, OsCKX2 gene was found to be up regulated in the leaves of 18℃ and OsIAA1 gene could be expressed more in the stem of 24℃.

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Effect of Ginsenosides from Panax ginseng on Proliferation of Human Osteosarcoma Cell $U_2OS$

  • Deqiang Dou;Jie Ren;Yingjie Chen;Youwei Zhang;Xinsheng Yao
    • Proceedings of the Ginseng society Conference
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    • 2002.10a
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    • pp.376-384
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    • 2002
  • Object To find out which of the 27 ginsenosides isolated from Panax ginseng C.A. Mey that may inhibit the proliferation of human osteosaocoma cell line $U_2OS$. Methods Effects of each individual ginsenoside on the proliferation of $U_2OS$ cell were studied by determining the viability of cancer cells during culture with or without the presence of the test compound. DNA assay was determined by flow cytometry. Results Ginsonosides -Ro, $-Rh_l,\;-Rh_2,\;-F_1\;and\;-L_8$ at concentrations of 5 ,umol/L could obviously suppress the proliferation of $U_2OS$ cells while ginsenosides $-Rg_1,\;-F_3,$ -Rf, PPT and PT significantly inhibited the cancer cells. Flow cytometry revealed that ginsenosides $-Ro,-Rg_1-Rf,-F_1-Rh_2,PPT$ and PT induced cell cycle arrest at $G_0/G_1$ phase with obvious decrease of cell count at Sand $G_2+M$ phase, Moreover, ginsenosides $-Rf_1,-Rg_1,\;-F_1$ and PPT induced significantly high rates of cell death as compared with the control. Conclusion These data suggested that ginsenosides inhibited $U_2OS$ proliferation Via cell cycle arrest or induction of cell death.

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