• 한국환경농학회지
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• 제33권3호
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• pp.194-197
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• 2014

BACKGROUND: Herbal extracts have been screened for their inhibitory effect of seed germination and root development on weeds, but there is a scarcity of reports for crop growth regulation. The objective of this research was to develop a growth inhibitor on Brassica campestris, and its effective extraction method from herbal medicine extract. METHODS AND RESULTS: Eighty four herbal medicine extracts were tested for their plant growth inhibition activity on B. campestris. The alcohol extracts of Artemisia annua, Cinnamomum cassia, and Mentha arvensis inhibited over 30% of germination and the extract of A. annua, and C. cassia inhibited over 70% of radicle growth at 0.1 % w/w treatment. The partially purified extracts of A. annua, and C. cassia with dichloromethane and hexane showed stronger radicle growth inhibition than the crude extracts on B. campestris. The diethyl ether extract of A. annua showed a similar 50% radicle growth inhibition ($RI_{50}$ = 45 mg/L) to its partially purified extract with dichloromethane or hexane, but the diethyl ether extract of C. cassia showed a worse $RI_{50}$ than the purified extract. CONCLUSION: The alcohol extracts of A. annua, and C. cassia showed potent radicle growth inhibition properties on B. campestris. Diethyl ether proved to be a good solvent for simple extraction from A. annua.

• 동의생리병리학회지
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• 제22권3호
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• pp.619-623
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• 2008

The purpose of this study was to observe the effects of antibacterial activities on various medicinal herbs. Medicinal herbs used this study was Curcuma aromatica, Scutellaria baicalensis, Angelica dahurica, Platycodon grandiflorum, Houttuynia corolata, and Forsythia saxatilis. To evaluation of the antibacterial effect, we used S. gallinarum(ATCC 9184), S. typhimurium(SAL-13, France), S. enteritidis (ATCC 10376), Escherichia coli(E-62, O78), and S. gallinarum(E. coli). Extracts from each medicinal herbs divided into 100mg/ml, 50 mg/ml, 25 mg/ml, and 12.5 mg/ml. Antibacterial activities achieved by the paper disc test. Antibacterial activities was showed at extracts from Scutellaria baicalensis, Houttuynia corolata, Forsythia saxatilis, but little found at Curcuma aromatica, Angelica dahurica, Platycodon grandiflorum.

• 한방비만학회지
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• 제15권2호
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• pp.123-130
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• 2015

Objectives: Samjung-hwan (SJH), a well-known traditional fermented herb formula recorded in Dongui Bogam, has been commonly used for prolonging life for four hundred years in Eastern Asia. However, fermented SJH has not been investigated in terms of microbial ecology until present time. Methods: SJH was fermented for five weeks and fermentation characteristics during SJH fermentation were performed including pH, acidity and microbial profiling. Also, we measured total polyphenol and total flavonoid contents and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. In order to select starter candidate, several lactic acid bacteria were isolated from fermented SJH. Results: pH of fermented SJH was decreased from 4.7 to 3.0 and acidity was increased from 0.45% to 1.72%. Also, fermented SJH increased antioxidant indicator such as total polyphenol and total flavonoid as well as DPPH free radical scavenging activity. Lactobacillus brevis was increased, Pseudanabaena sp. was decreased, and Lactococcus lactis subsp. lactis was stable during 5-week fermentation of SJH. L. brevis and L. plantarum were isolated from fermented SJH. Conclusions: Fermented SJH for four weeks had optimal effect on antioxidant and fermentation characteristics such as pH, acidity and microbial profile. Further studies are required to develop starter and analyze functional compounds in oder to produce standardized SJH.

• 대한한방부인과학회지
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• 제31권4호
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• pp.54-72
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• 2018

Objectives: The aim of this review is to investigate studies on Estrogen-like activity and to contribute to the utilization of herbal medicines including phytoestrogens. Methods: Electric searches were performed with Pubmed from 2013 to June 2018, for the words, 'herb and estrogen', 'traditional Chinese medicine and estrogen', 'Oriental medicine and estrogen', and 'Korean medicine and estrogen'. 49 papers are investigated and classified into 'in vitro', 'in vivo' and 'in vivo and in vitro' experimental studies. Results: 1. In vitro experimental studies have shown that estrogen-like effects of the components extracted from Rhei Radix et Rhizoma, Rubi Fructus, Sparganii Rhizoma, Epimedii Herba, Spatholobi Caulis, Evodiae fructus, Curcumae longae Radix, Ginseng Radix, Bupleuri Radix, Astragali Radix, Salviae Miltiorrhizae Radix, Puerariae Radix, Scutellariae Radix are present. 2. In vivo experimental studies have shown that estrogen-like effects of the components extracted from Phytolaccae Radix, Ligustri Lucidi Fructus, Alismatis Rhizoma, Notoginseng Radix, Puerariae Radix, Ginseng Radix, Cyperi Rhizoma, Cistanchis Herba, Cynomorii Herba, Granati fructus, Astragali Radix, Rehmanniae Radix Crudus, Epimedii Herba, Polygalae Radix, Artemisiae Annuae Herba are present. 3. In vitro and in vivo experimental studies have shown that estrogen-like effects of the components extracted from Cirsii Herba, Dioscoreae Rhizoma, Salviae Miltiorrhizae Radix, Cynomorii Herba, Cinnamomi cortex, Drynariae Rhizoma, Psoraleae Semen, Schisandrae Fructus, Epimedii Herba, Astragali Radix are present. Conclusions: Future studies will require additional research on numerous herbal medicines used in clinical practice.

• 약학회지
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• 제47권3호
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• pp.167-175
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• 2003

Eleutherococcus senticosus is a typical oriental folk medicinal herb. It has been used clinically as a anti-rheumatic disease, anti-stress, ischemic heart disease and gastric ulcer. In the present study, we examined the adjuvant activity of the crude polysaccharides fraction from Eleutherococcus senticosus, EN-3, on the induction of humoral and cellular immune responses against bovine serum albumin (BSA) or ovalbumin (OVA). The thioglycollate-induced macrophages and silica-induced dendritic-like cells cultured with BSA and EN-3 synergistically increased the production of TNF-$\alpha$ and IL-12. When mice were subcutaneously immunized with BSA + EN-3, the antibody titer against BSA was showed significantly higher than those immunized with BSA alone. In addition, when mice were immunized with OVA + FIA + EN-3, the antibody titer was showed similar patterns with the FCA. The assay for determining subisotype of antibody revealed that EN-3 augmented OVA-specific antibody titer of IgG1 and IgG2b. The culture supernatant obtained from splenocytes of mice treated with OVA + FIA + EN-3 also showed a higher level of both OVA-specific Th1-type (IL-2, IFN-${\gamma}$ and GM-CSF) and Th2-type cytokine (IL-4, IL-6 and IL-10). In vitro analysis of T cell proliferation to OVA on 8 weeks, the splenocytes of mice treated with OVA + EN-3 showed a significantly higher proliferating activity than those treated with OVA alone. These results suggest that EN-3 may possess adjuvant activities to potentially to enhance humoral as well as cellular immune response.

• 대한한의학방제학회지
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• 제23권2호
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• pp.209-224
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• 2015

Objective : The main purpose of this study is to find a reasonable solutions for utilization of herbal formulas based on the medical classics. Methods : We used official Ten 'Selected Korean Medical Classics(SKMC)'. For input of formulas from SKMC, we had to select proper editions of them. After consultations with experts of 'The Korean Medicine Society for the Herbal Formula Study' and 'Korean Society of Medical History', we chose the proper editions of SKMC, and according to those books, we built formulas database using microsoft office excel sheets, respectively. Results : After whole input procedures, we requested several experts to inspect the results and also, to correct errors in them. Input categories were name of formula, name of classic, classification, original source, composition herb, weight, unit of weight, weight conversion index, converted weight, basic formula, indications, reason for modification, preparing method, taking method and contraindications. Final number of whole formulas in DB was 32,882. Conclusions : We used SKMC and built database using excel program. Through this procedure, we can provide a developmental ways to make a foundation for herbal formulas from the medical classics, so that we can make herbal formulas be standardized or industrialized more efficiently.

• 대한화장품학회지
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• 제33권2호
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• pp.127-135
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• 2007

본 연구는 한방화장품의 원료로써 사용이 되는 약용식물들의 부위별 및 추출용매에 따른 추출물의 효능의 차이를 기술하였다. 기존의 단일성분 분석법보다 실제로 성분의 복합체인 추출물에 대하여 항산화와 UV흡수 관련 효능효과 시험법을 적용하였다. 약효를 가지는 약용식물들을 추출할 때 고려해야 할 조건이 있다. 같은 약용식물이라도 채취시기, 채취장소, 채취부위와 같은 채취조건들과 용매의 종류, 추출시간, 추출온도와 같은 추출조건들을 고려하여야 한다. 조건 중에서 추출용매와 채집부위는 효과에 있어서 매우 중요한 요인이다. 약용식물에 대한 관심이 증가하면서 여러 가지의 채취조건과 추출조건을 연구하여 한방원료의 개발에 있어 좀더 다양한 적용이 요청되고 있다. 본 연구에서는 약용과 식용으로 널리 사용되는 식물(Terminalia chebula, Syzygium aromaticum, Paeonia lactiflora, Morus alba, Scutellaria baicalensis)을 선정하여 추출용매와 채집부위를 변경시킴으로써 용매와 부위에 따른 효능의 차이를 확인하였다. 이 결과로 추출시 적당한 조건을 선택한다면 사용되는 약용식물의 양을 줄이거나 좀더 효과적인 결과를 얻을 수 있다.

• 동아시아식생활학회지
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• 제13권4호
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• pp.293-304
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• 2003

This study was performed to investigate the effects of peking-duck extracts added with medicinal herbs(DJ) on the intoxication of heavy metals (Hg, Pb, Cd, As) in rats. Sprague-Dawley rats weighing 150$\pm$15g, were randomly assigned to groups: basal diet only in normal control group(NCG): basal diet and heavy metals without DJ injection in heavy metal control group(HMC); basal diet, heavy metals and DJ(3mg/ml) injection in heavy metal low duck-juice group(HMLD), basal diet, heavy metals and DJ(30mg/ml) injection in heavy metal middle duck-juice group(HMMD): basal diet, heavy metal and DJ(300mg/ml) injection in heavy metal high duck-juice group(HMHD). Hg and As was injected by 50ppm and Cd and Pb by 25ppm for 17days. Also DJ oral feeding was conducted for 28days. The result of this study were as follows: Food intake and body weight gain in heavy metal administered groups were lower than those of NCG. Liver, kidney and testis weights were not significantly different among 5 groups. GOT, GPT and BUN activities were significantly reduced in DJ treated groups as compared to HMC. DJ showed the suppressing effect on the accumulation of Hg, Pb and Cd in serum, liver and kidney. Fecal Hg and Cd excretions increased with DJ feeding. The results suggested that DJ may have some protective effects on Hg, Cd and Pb intoxication by reducing the accumulation in tissues and increasing excretion. This study also showed the effective way of using duck-extract and its application to the oriental medicine.

• 식물분류학회지
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• 제45권2호
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• pp.192-212
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• 2015

울릉도에 자생하는 식물상을 밝히고 주요 식물들의 분포를 파악을 위해 2007년부터 2011년에 걸쳐 총 55일간의 현지조사를 수행하였다. 현지조사 결과 조사지역의 관속식물 93과 313속 462종 6아종 52변종 4품종 등 총 494분류군을 확인하였다. IUCN 기준에 따른 산림청 지정 희귀식물은 멸종위기종(CR) 9분류군, 위기종(EN) 6분류군, 취약종(VU) 12분류군, 환경부 지정 멸종위기 야생식물은 1급 1분류군, 2급 3분류군, 식물구계학적 특정식물 중 III등급 이상에 해당하는 71분류군의 분포를 확인하였으며 울릉도 특산식물은 33분류군으로 정리되었다. 귀화식물은 66분류군이 조사되었고 생태계교란종은 애기수영, 돼지풀, 가시상추 3분류군이 확인되었다.

• 대한화장품학회지
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• 제42권3호
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• pp.203-209
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• 2016

최근 남성성탈모증에 대한 관심이 증대되고 있으며, 이에 천연물 및 그의 활성성분을 활용한 새로운 약물 개발에 대한 연구가 증가하고 있다. 송지(Resina Pini, RP)는 Pinus sp. (Pinaceae)의 수지질로 전통의학적으로 감염, 우식증, 치주질환에 사용되어왔다. 본 연구진은 RP의 성분인 아비에트산(abietic acid, AA)이 남성성탈모기전에 중요한 효소인 $5{\alpha}$-reductase를 억제하는 효과를 세포 수준에서 입증한바 있으며, 이번 연구에서는 실제로 탈모억제 및 모발 성장에 대하여 실험동물 수준에서 입증하고자 한다. C3H/HeN 탈모마우스 모델에서 RP는 300 mg/kg에서 유의하게 탈모억제를 확인하였으며, 뿐만 아니라 AA는 30 mg/kg에서도 유의하게 탈모억제효과를 보였다. 이상의 결과로부터 RP는 그 활성성분인 AA가 $5{\alpha}$-reductase 억제하는 기전을 통해 남성성탈모억제효과를 보였다고 사료되며, 향후 탈모억제 보완치료법으로의 이용 가능성을 보였다.