• Korean Journal of Soil Science and Fertilizer
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• v.41 no.6
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• pp.415-418
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• 2008

The application of sludge wastes into agricultural fields has been increasing annually in Korea. In particular, sewage sludge application has been widely accepted in decades. Sewage sludge application aid in the recycling of essential nutrients and act as a source of organic matter improving the structure and water-holding properties of the soil. The efficient use of sludge wastes, however, requires an individual assessment of waste products. This study assessed the biological characteristics of organic waste-treated lysimeter soils and develop its indicator to assess the soil health of organic waste-treated lysimeter soils. Several analytical techniques more recently developed such as restriction fragment length polymorphism (RFLP), phospholipid fatty acid (PLFA), and community level substrate utilization (CLSU) fingerprints allow for detailed analyses of soil microbial communities. PLFA and RFLP was, therefore, used in the study to characterize the microbial communities in soil without the need to isolate individual fungi and bacteria. PLFA, RFLP and CLSU have been utilized to assess microbial characteristics of the lysimeter soils with four different sludge wastes for eight consecutive years. Each of these methods was analyzed for a different aspect of soil microbial characteristics. The study would disclose those methods yielded highly reproductive results for each soil and allow distinguishing the soils based on the structures of specific geneand PLFA-pools more than CLSU fingerprints. PLFA methods, especially, revealed the same relative similarities of the treated soils based on cluster analysis of the biological characteristics. Pig manure compost-treated soil, however, was only the same relative resemblance among the three methods. These results indicated that PLFA easily assessed the biological soil characterization.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.1
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• pp.65-72
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• 2013

This study was carried out to understand the long-term effects of organic waste treatments on the fate of heavy metals in soils originated from the organic wastes and consequent uptake of heavy metals by plant, together with examination of changes in soil properties and plant growth performance. In this study, the soils treated with three different organic wastes (municipal sewage sludge, alcohol fermentation processing sludge, pig manure compost) at three different rates (12.5, 25.0, 50.0 ton $ha^{-1}yr^{-1}$) for 7 years (1994 - 2000) were used. To see the long-term effect, plant growth study and soil examination were conducted twice in 2000 and 2010, respectively. There was no additional treatments of organic wastes for 10 years after the organic waste treatment for 7 years. Compared to plant growth examination conducted in 2000 using radish (Raphanus sativus cv. sodamaltari), it appeared that height, root length and diameter, fresh weight of radish grown in 2010 decreased in the plots treated with municipal sewage sludge and alcohol fermentation processing sludge and that the extent of decrease was higher with increase of sludge application rates. On the other hand, pig compost treatment increased plant height, root length and diameter, fresh weight with increasing application rates. Cu and Pb concentrations in radish root and leaves increased in 2010 compared to those in 2000 while Ni concentrations in root and leaves decreased. Zn concentration was increased only in the soils treated with pig manure compost. Multiple regression analysis among heavy metal species fractions in soils, soil pH, and metal concentrations in radish root and leaves indicated that the metal uptake by radish was governed mainly by the soil pH and subsequent increase of available heavy metal fractions in soils with organic waste treatments.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2019.10a
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• pp.134-134
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• 2019

Utilization of organic waste as a renewable energy source is promising for sustainability and mitigation of climate change. Pyrolysis converts organic waste to gas, oil, and biochar by incomplete biomass combustion. Biochar is widely used as a soil conditioner and adsorbent. Biochar adsorbs/desorbs metals and ions depending on the soil environment and condition to act as a nutrient buffer in soils. Biochar is also regarded as a carbon storage by fixation of organic carbon. Phosphorus (P) and nitrogen (N) are strictly controlled in many wastewater treatment plants because it causes eutrophication in water bodies. P and N is removed by biological and chemical methods in wastewater treatment plants and transferred to sludge for disposal. On the other hand, P is an irreplaceable essential element for all living organisms and its resource (phosphate rock) is estimated about 100 years of economical mining. Therefore, P and N recovery from waste and wastewater is a critical issue for sustainable human society. For the purpose, intensive researches have been carried out to remove and recover P and N from waste and wastewater. Previous studies have shown that biochars can adsorb and desorbed phosphates implying that biochars could be a complementary fertilizer. However, most of the conventional biochar have limited capacity to adsorb phosphates and nitrate. Recent studies have focused on biochar impregnated with metal salts to improve phosphates and nitrate adsorption by synthesizing biochars with novel structures and surface properties. Metal salts and metal oxides have been used for the surface modification of biochars. If P removal is the only concern, P adsorption kinetics and capacity are the only important factors. If both of P and N removal and the application of recovery are concerned, however, P and N desorption characteristics and bioavailability are also critical factors to be considered. Most of the researches on impregnated biochars have focused on P removal efficiency and kinetics. In this study, coffee waste is thermally treated to produce biochar and it was impregnated with Mg/Al to enhance phosphates and nitrate adsorption/desorption and P bioavailability to increase its value as a fertilizer. Kinetics of phosphates and nitrate adsorption/desorption and bioavailability analysis were carried out to estimate its potential as a P and N removal adsorbent in wasewater and a fertilizer in soil.

• Journal of Mushroom
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• v.5 no.1
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• pp.43-49
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• 2007

Application of sludge wastes into the field may help soil fertility with physical, chemical and biological properties. Efficient use of sludge waste, however, requires an individual assessment of the waste products. A lot of experiment into the organic waste-treated soils has been done for decade. However, studies have not been carried out on the assessment of agricultural soil by Basidiomycota. This study was assessed the influence of sludge application on soil healthy in agricultural upland soils. The organic wastes selected for long-term application experiment in this study were municipal sewage sludge (MSS), industrial sewage sludge (ISS), leather processing sludge (LS), alcohol fermentation processing sludge (FS), and pig manure compost (PMC). To develop the soil healthy assesment method, soil samples were diluted by 20X with distilled water. After shaking at 200rpm for 30 minutes, the shaked sample was mixed on PDA(Potato Dextrose Agar). And sterilized at $121^{\circ}C$ for 20 minutes. Coriolus hirsutus (MKACC 50560) was inoculated on petri-dish including PDA mixed sample. After the media was incubated at $25^{\circ}C$ for five days, the mycelial growth of C. hirsutus was measured. When the mycelial growth on sample media was compared with growth on media contained PDA only, well grown media contained sample soil was assesed as healthy soil. The results suggest that the simple method by Coriolus hirsutus is a handy way to assess the healthy of waste sludge-applied upland soils.

• Korean Journal of Soil Science and Fertilizer
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• v.17 no.1
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• pp.55-59
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• 1984

An organic compound fertilizer was manufactured using wet oxidation human waste as principal source of phosphorus and organic matter. The waste was treated with sulfuric and glutamic acids to increase the available and water-soluble $P_2O_5$ contents. The treatment of 0.1 N sulfuric acid with 24 hours curing was best way in recovering the maximum percentage of $P_2O_5$ originally in the waste. The particle size distribution of trial product varied considerably in the amount of glutamic acid used for granulation. The number of relatively large fertilizer particles was increased as the amount of glutamic acid was increased. The granule crushing strength was generally high in large granules in which 12.5 weight percent of glutamic acid were used for granulation. The trial product showed high moisture absorption due to its porous structure and chemical makeup.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.6
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• pp.500-512
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• 2009

This study was carried out to evaluate the effect of temperature on soil microbial biomass, enzyme activities, and PLFA content in the volcanic(VAS) and the non-volcanic ash soil(NVAS). The soils were treated with organic materials such as organic fertilizer pelleted(OFPL), organic fertilizer powdered(OFPD), pig manure compost(PMC), and food waste compost(FWC). Two grams of organic materials were well mixed with 30g of dried volcanic and non-volcanic ash soil(< 2 mm) with 50% of soil moisture content. And the soils were incubated at 10, 20, $30^{\circ}C$ in incubator. Soils were analysed on the incubation times as followed; soil pH, total nitrogen, organic matter(at 75, 150, 270 days), microbial biomass C and PLFA (at 75, 270 days), microbial biomass N and soil enzyme(at 150, 270 days). pH values of soils treated with PMC and FWC had no changes on soil type, and incubation temperature. However, the pH was increased with temperature in the soils treated with OFPL. The changes in NVAS was higher than in VAS. Soil microbial biomass C content were high in the condition of high temperature and organic fertilizers treatment in VAS. But the contents were gradually decreased with incubation period in both NVAS and VAS. Soil microbial biomass N was high in NVAS treated with organic fertilizers and in VBS treated with PMC and FWC. PLFA content was higher in NVBS than in VBS at 75 days but showed high in VBS at 270 days. Urease activity of NVBS treated with OFPL showed $10^{\circ}C$ (75.0)> $20^{\circ}C$ (16.3)>$30^{\circ}C$ ($4.6ug\;NH{_4-}N\;g^{-1}\;2h^{-1}$) at 150 days. It were decreased gradually high temperature and time passes. And it showed high at $10^{\circ}C$ in VBS. Glucosidase activity was higher in NVBS than in VBS. Correlation coefficient of between soil microbial biomass C and microbial activity indicators showed that PLFA was high significantly at $r^2=0.91$ in NVBS and ${\beta}-glucosidase$ was $r^2=0.83$ in VBS. Soil microbial activities showed differences in the relative sensitivities of soil type and soil temperature.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.1
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• pp.51-56
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• 2007

The toxicities of contaminated soils with 8 consecutive year applications of three levels (12.5, 25.0, and $50.0t\;dry\;matter\;ha^{-1}yr^{-1}$) of four organic sludge [municipal sewage sludge (MSS), industrial sewage sludge (ISS), alcohol fermentation processing sludge (AFPS) and leather processing sludge (LPS)] on earthworm (Eisenia fetida) were examined by using microcosm container in the laboratory. Results were compared with those of pig manure compost (PMC) treated soil. In tests with three treatment levels (12.5, 25.0, and 50.0 t per plot), ISS treated soil showed higher contents of Cu (18.9~26.2 fold), Cr (7.7~34.7 fold), and Ni (14.8~18.8 fold) at 8 years post treatment, than PMC treated soil. LPS treated soil showed higher contents of Cr (35.7~268.0 fold) and Ni (4.5~7.6 fold) than PMC treated soil. There were no great differences in heavy metal contents among MSS, AFPS, and PMC treated soils. In these contaminated soils, earthworm mortalities of MSS and AFPS treated soils at 8 weeks post-exposure were similar to those of PMC treated soil regardless of each treatment level. Toxic effect (26.7~96.7 mortality) on the ISS and LPS treated soils was significantly higher than one of PMC treated soil, with an exception of LPS soil treated with 25.0 t per plot. At 16 weeks post-exposure, earthworm mortalities of AFPS' 12.5 and 25.0 t treated soils were similar to those of PMC treated soil. Toxic effect (53.3~100 mortality) on the 12.5, 25.0, and 50.0 t treated soils of MSS, ISS and LPS, and AFPS' 50.0 t treated soils was significantly higher than those of PMC treated soil. The data suggested that the 12.5, 25.0, and 50.0 t of MSS, ISS and LPS, and AFPS' 50.0 t treated soils were evaluated to have toxicity on earthworm.

• Journal of the Korean Wood Science and Technology
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• v.46 no.6
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• pp.645-655
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• 2018

The use of wood waste as substrate for plant growth exemplifies a strategy for turning waste into resources. The overall objective of this research was to evaluate the effects of wood-based growing media on plant cover in a slope area. Moreover, we tried to find out what physicochemical properties affect plant cover on a slope. For treatments, we tested natural soil, soil mixed with wood-based growing medium (1:1, w/w), and wood-based growing medium by itself. Physical and chemical characteristics were evaluated after four months from the date of treatment application to the experimental slope site. Soil coverage with seedlings of Lespedeza cyrtobotrya was measured for plant growth evaluation. Physicochemical properties were altered by mixing the natural soil with wood-based growing medium. Particularly, soil moisture and organic matter contents were significantly changed in soils treated with wood-based growing medium compared to soil alone. We confirmed that plant coverage rate was high when wood-based growing medium was mixed with the natural soil. There was a significant linear relationship between moisture content and CEC (Cation Exchange Capacity) of all growth media tested and plant coverage. This result was expected, as moisture content tends to increase with organic matter content, such as in wood-based growing medium. In conclusion, the high moisture content of the wood-based growing medium was considered effective for plant growth in the experimental slope site, and this wood-based growing medium provides a means to improve the harmony between the slope and the surrounding environment.

• Korean Journal of Environmental Biology
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• v.36 no.4
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• pp.471-478
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• 2018

The purpose of this study was to investigate effects of reusable organic wastes, on soil organisms and chemical properties. Bone meal, de-oiled cake, and oyster shell were applied and abundances of soil organisms were measured at 3 and 15 months, after treatment in soil under sawleaf zelkova cultivation. Soil organisms are affected, by the quality of applied organic wastes. Abundance of microorganisms was higher in oyster shell treated soils than in untreated soils, suggesting that soil pH is a driving force, altering abundance and structure of the microbial community. Increase in abundance of bacterivorous nematodes was observed under treatment with bone meal and de-oiled cake, but there was slight change in abundance of fungivorous and herbivorous nematodes. Abundance of herbivorous nematode was positively correlated with plant growth, at 15 months after treatment. Response of microarthropods in the Collembola and Oribatida was not apparent. Abundances of primary consumers were not significantly correlated with microorganisms. This study suggested that organic waste influences soil organisms primarily by altering soil chemical properties and bottom-up effects may not occur in trophic reactions.

• Korean Journal of Environmental Agriculture
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• v.34 no.2
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• pp.91-97
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• 2015

BACKGROUND: Earthworms are essential detritus feeders that play a vital role in the process of decomposition of organic matter and soil metabolism. The complex process of partial breakdown of organic matter and mixing with mucous and gut microbial flora in the form of earthworm cast results in the reduction of the toxicity. This study focused on the change of cast amount and pollutant contents before and after the eating of the organic waste and upland soil with the two species of earthworm. METHODS AND RESULTS: The two species of earthworms were compared to the cast production. In the upland soil material, the daily amount of worm's cast was 1.42 g in E. andrei and 0.40 g in A. agrestis. In the organic waste material, the cast of E. andrei was 0.78~0.83 g and the cast of A. agrestis. have not been collected because all earthworms died after the treatment. The heavy metals treated in the upland soil were evaluated the impact of the worm excretion. With the E. andrei, the cast production was decreased 0.1~0.8 times in zinc, 0.2~0.5 times in copper, and 0.1~0.7 times in cadmium compared to the control treatment according to the levels of concentration. With A. agrestis, the cast amount was decreased 0.3~1.1 times in zinc, 0.2~0.3 times in copper, and 0.1~2.1 times in cadmium, respectively. The changes of pollutant contents before and after the eating of the organic wastes with E. andrei were studied. In the treatment of the Alcohol Fermentation Processing Sludge and the Fruit Juice Processing Sludge, heavy metal content of the cast was increased 0.7~53.3% compared to the sludge materials. PAHs contents were decreased 50.1% in the cast of the Alcohol Fermentation Processing Sludge and 36.6% in the cast of the Fruit Juice Processing Sludge, respectively. CONCLUSION: In conclusion, although the A. agrestis was bigger than E. andrei in size and weight, the cast amount of A. agrestis was small. The two species of earthworm was less excretion with high concentration of heavy metals. While the heavy metals such as zinc, copper, and cadmium were considerably accumulated in the cast, the total compounds, PAHs were fairly decomposed. There results would provide us for restoring contaminated soil and cleaning organic wastes.