• Title/Summary/Keyword: Organic media

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Optimal Condition for Mycelial Growth of Beauveria bassiana and Its Extracellular Enzyme Activity (백강균(Beauveria bassiana)의 균사체 최적 배양조건 및 효소활성)

  • 민응기;한영환
    • Korean Journal of Microbiology
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    • v.38 no.1
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    • pp.50-53
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    • 2002
  • The optimum temperature and pH for mycelial growth of B. bassiana DGUM 34001 were $24^{\circ}C$ and pH 7.0, respectively. Among the complex media used, mushroom complex medium (MCM) was the most favorable for mycelial growth. When Czapek-Dox medium was used as a minimal medium, glucose was an excellent source for carbon and energy. Soytone and sodium phosphate were favorable constituent for culture medium as a source of organic nitrogen and phosphorus, respectively. When the fungus was grown in MCM broth, the specific activity of extracellular enzyme of ${\alpha}$-amylase, lipase, chitinase, CMCase and pretease were 297.0, 0.058, 0.33, 0.21 and 22.8 units/mg protein, respectively. When various sources of organic nitrogen and chitin were supplemented to determine the production of enzymes, casein and soluble chitosan enhanced the production of extracellular protease and chitinase.

Use of biofilter as pre-treatment of polluted river water for drinking water supply

  • Suprihatin, Suprihatin;Cahyaputri, Bunga;Romli, Muhammad;Yani, Mohamad
    • Environmental Engineering Research
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    • v.22 no.2
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    • pp.203-209
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    • 2017
  • Innovations in the biofiltration process can provide effective solutions to overcome crucial water pollution problems. The elimination of pollutants is a result of the combined effects of biological oxidation, adsorption and filtration processes. This research aims to evaluate the performance of quartz sand biofiltration for removing total suspended solids, turbidity, color, organic matter, and ammonium from polluted river water and develop an empirical model for designing quartz sand biofilters for the treatment of polluted river water. Experiments were conducted using two biofilter units filled with quartz sand as filter media. A set of experiments were performed to evaluate the effect of hydraulic retention time on biofilter performance in removing water contaminants. The kinetics of organic matter removal were also determined to describe the performance of the biofilter. The results show that biofiltration can significantly remove river water pollutants. Removal efficiency depends on the applied hydraulic retention time. At a hydraulic retention time of two hours, removal efficiencies of total organics, ammonium and total suspended solids were up to 78%, 82%, and 91%, respectively. A model for designing quartz sand biofiltration has been developed from the experimental data.

Decomposition of Toxic Chemicals in Microemulsion by Electrolytic Oxidation Method (마이크로 에멀젼 상태에서 전기분해법을 이용한 독성물질 분해 연구)

  • Shim, Sung-Hyun;Chun, Byoung-Chul;Chung, Yong-Chan
    • Clean Technology
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    • v.14 no.3
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    • pp.218-223
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    • 2008
  • Decomposition of reactive organic compound dispersed in microemulsion media by hydroxide ions and proton ions generated during electrolysis was tried and the half-lifes for decomposition were compared. Absorbance of p-nitrophenoxide produced from the decomposition of p-nitrophenylacetate (PNPA) was followed to find the rate of decomposition. The applied voltage, temperature, and the amount of substrate were changed to see the effects on the decomposition rate. The advantages of electrolysis in microemulsion system were the high solubilizing capacity of substrate, easy control of decomposition rate, low operation cost, no need for any addition of chemicals, and no byproducts. The mechanism of decomposition and the application to water purification were discussed.

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Effect of organic acids in dental biofilm on microhardness of a silorane-based composite

  • Hashemikamangar, Sedighe Sadat;Pourhashemi, Seyed Jalal;Talebi, Mohammad;Kiomarsi, Nazanin;Kharazifard, Mohammad Javad
    • Restorative Dentistry and Endodontics
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    • v.40 no.3
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    • pp.188-194
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    • 2015
  • Objectives: This study evaluated the effect of lactic acid and acetic acid on the microhardness of a silorane-based composite compared to two methacrylate-based composite resins. Materials and Methods: Thirty disc-shaped specimens each were fabricated of Filtek P90, Filtek Z250 and Filtek Z350XT. After measuring of Vickers microhardness, they were randomly divided into 3 subgroups (n = 10) and immersed in lactic acid, acetic acid or distilled water. Microhardness was measured after 48 hr and 7 day of immersion. Data were analyzed using repeated measures ANOVA (p < 0.05). The surfaces of two additional specimens were evaluated using a scanning electron microscope (SEM) before and after immersion. Results: All groups showed a reduction in microhardness after 7 day of immersion (p < 0.001). At baseline and 7 day, the microhardness of Z250 was the greatest, followed by Z350 and P90 (p < 0.001). At 48 hr, the microhardness values of Z250 and Z350 were greater than P90 (p < 0.001 for both), but those of Z250 and Z350 were not significantly different (p = 0.095). Also, the effect of storage media on microhardness was not significant at baseline, but significant at 48 hr and after 7 day (p = 0.001 and p < 0.001, respectively). Lactic acid had the greatest effect. Conclusions: The microhardness of composites decreased after 7 day of immersion. The microhardness of P90 was lower than that of other composites. Lactic acid caused a greater reduction in microhardness compared to other solutions.

Analysis of Pathogenic Microorganism's Contamination and Heavy Metals on Kimchi Cabbage by Cultivation Methods in Korea (재배농법에 따른 국내산 배추의 위해미생물 및 중금속 오염평가)

  • Oh, Soh-Young;Nam, Ki-Woong;Yoon, Deok-Hoon
    • Journal of Food Hygiene and Safety
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    • v.32 no.6
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    • pp.500-506
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    • 2017
  • Kimchi cabbage is one of the four major vegetable crops in Korea. The total annual production of kimchi cabbage, the main material of kimchi, was 20,559 tons in 2015. Kimchi cabbage is one of the majer crops produced by farmers which accounts for about 80% of the total leaf vegetable production in Korea. As the consumption of environmental-friendly agricultural products increases, food safety is one of the major public health concerns. We analyzed the biological hazards of kimchi cabbage produced by two types of cultivation methos such as organic farming and conventional farming using various culture media and microscopy. A total of 432 samples were analysed for presence of sanitary indicator microorganisms (aerobic plate count, coliform count, yeast & mold) and food-borne pathogens (Staphylococcus aureus, environmental Listeria, Bacillus cereus). The population of sanitary indicating microorganisms and food borne pathogens was under 5 Log CFU/g in all tested samples. The results of total microorganism numbers of leaf surface showed a positive correlation to those of soil samples. Additionally, we examined chemical factors such as pesticide residues and heavy metals in soil samples. All tested samples did not shown contamination levels higher than the standard limit.

Study on the Isolation and Characterization of Cellulose degrading Microorganism from Cocopeat (코코피트로부터 분리한 섬유소분해세균의 분리, 동정 및 특징에 관한 연구)

  • Chang, Jea-Eun;Kim, Jin-Whan;Kim, Young-Jun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.19 no.4
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    • pp.84-89
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    • 2011
  • Cellulose-degrading bacteria were isolated and identified from cocopeat which has a good quality as a bulking agent in composting. Various bacteria from different sourecs of cocopeat were detected on CMC agar media, and these were found to be Burkholderi2a sp., Bacillu subtilis, Sphingomonas sp., Rhodotorula sp. & Pseudomonas sp. etc. Among these, four bacteria were further selected and analyzed for their biochemical characteristics and CMCase activities. CMCase activities of four bacteria, P. aeruginosa, P. stutzeri, B. subtilis, and P. luteola were found to be 83%, 40%, 8%, 6%, respectively, compared with that of the standard strain Cellulomonas sp.

Bacterial Stringent Signal Directs Virulence and Survival in Vibrio cholerae.

  • Oh, Young Taek;Kim, Hwa Young;Yoon, Sang Sun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.8-8
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    • 2019
  • The stringent response (SR) is characterized as a bacterial defense mechanism in response to various growth-inhibiting stresses. It is activated by accumulation of a small nucleotide regulator, (p)ppGpp, and induces global changes in bacterial transcription and translation. Recent work from our group has shown that (p)ppGpp plays a critical role in virulence and survival in Vibrio cholerae. The genes, relA and relV, are involved in the production of (p)ppGpp, while the spoT gene encodes an enzyme that hydrolyzes it in V. cholerae. A mutant strain defective in (p)ppGpp production (i.e. ${\Delta}relA{\Delta}relV{\Delta}spoT$ mutant) lost the ability to produce cholera toxin (CT) and lost their viability due to uncontrolled production of organic acids, when grown with extra glucose. In contrast, the ${\Delta}relA{\Delta}spoT$ mutant, a (p)ppGpp overproducer strain, produced enhanced level of CT and exhibited better growth in glucose supplemented media via glucose metabolic switch from organic fermentation to acetoin, a neutral fermentation end product, fermentation. These findings indicates that (p)ppGpp, in addition to its well-known role as a SR mediator, positively regulates CT production and maintenance of growth fitness in V. cholerae. This implicates SR as a promising drug target, inhibition of which may possibly downregulate V. cholerae virulence and survival fitness. Therefore, we screened a chemical library and identified a compound that induces medium acidification (termed iMAC) and thereby loss of wild type V. cholerae viability under glucose-rich conditions. Further, we present a potential mechanism by which the compound inhibits (p)ppGpp accumulation. Together, these results indicate that iMAC treatment causes V. cholerae cells to produce significantly less (p)ppGpp, an important regulator of the bacterial virulence and survival response, and further suggesting that it has a therapeutic potential to be developed as a novel antibacterial agent against cholera.

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A Study on the Characteristics of Low Temperature sintering Ceramic Siding Using Natural Minerals (천연광물을 활용한 저온소결 세라믹 사이딩의 특성에 관한 연구)

  • Kim, Soon-ho;Choi, Jeong-min
    • Journal of the Architectural Institute of Korea Structure & Construction
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    • v.35 no.12
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    • pp.149-156
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    • 2019
  • Recently, skyscraper building and apartment fires, which were rapidly spread out from a low floor to a rooftop, have become a frequent occurrence in mass media. This fire problems have a fatal disadvantage that the exterior wall finish of the building emits toxic gas in case of fire by using dry bit method or organic insulating material. Therefore, in order to remedy these problems, many exterior wall finishing construction methods have been proposed, but the current trend is to use existing construction methods due to problems such as economy, weight, and durability. On the other hand, in countries such as Germany and Japan, ceramic sidings are used as exterior finishing material for buildings, which is environmentally friendly, excellent natural beauty, long life, easy maintenance and high-quality exterior materials. However, those ceramic sidings have still the problems such as manufacturing cost and weight problem because of boosting the sintering temperature up to 1,350℃ or more. Also, conventional CRC, MgO, FRP sidings which are composed of pulp, glass fiber and organic materials, have been reports of deformation due to ultraviolet rays, discoloration, corrosion and scattering, surface rupture, lifting and peeling. Therefore, in this study as an alternative to solve this problem, halosite nano kaolin produced in Sancheong in Korea and frit flux were used to satisfy the required properties as ceramic siding using low temperature sintering (below 1,000℃) and lightweight materials such as pearlite. This study aims to design the optimal formulation and process of materials and to study the characteristics of nano-coated ceramic siding material development and to present relevant basic data. The findings show that ceramic siding for nanocoated building materials is excellent as a natural ceramic siding building material. The fire resistance of natural minerals and nano particle refining technology satisfy the bending strength of 80kgf / cm2, the volume ratio of 2.0 and the absorption rate of less than 10.0%.

Data Load Process of large-sized media for avionics using FTP and JSON (FTP와 JSON을 활용한 대용량 미디어의 항공장비용 데이터 로드 프로세스)

  • Ji-Hwan Choi;Nak-Min Choi;Jae-Kwon Shin
    • Journal of Advanced Navigation Technology
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    • v.27 no.5
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    • pp.610-620
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    • 2023
  • The interest in the aircraft interior market is gradually growing due to technological development based on the 4th industrial revolution and competition for airlines to attract customers, and as part of that, Cabin Display System (CDS) for FAA Part.25 civil aircraft is being developed in Korea. The CDS is a system that provides various multimedia services to passengers by utilizing Flexible and Transparent Organic Light Emitting Diodes (OLED) with Integrated Display Processing Module (IDPM). This paper presents a new method for efficient Data Load Process of large-sized files and deals with their implementation and performance. The results of this study are expected to be applied to Data Load Process development of avionics that require reliable large-capacity file transmission along with reducing the costs of development compared to existing ARINC-615A.

Effects of Column Boundary Flow and Surfactant Contents on Soil Hydraulic Conductivity (토양 칼럼의 경계흐름과 계면활성제가 수리전도도에 미치는 영향연구)

  • Jeong, Seung-Woo;Ju, Byung-Kyu
    • Journal of the Korea Organic Resources Recycling Association
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    • v.17 no.1
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    • pp.73-79
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    • 2009
  • The hydraulic conductivity of porous media is the most important property in soil characteristics. The hydraulic conductivity is determined by outdoor and indoor methods. Indoor methods normally use soil columns for flow test. Assumption of the column test is that fluid one-dimensionally flows through the column. However, fluids may flow toward the wall of the column, resulting in "boundary flow". This study investigated the effect of boundary flow on the hydraulic conductivity by using a permeameter excluding boundary flow. The results showed that the hydraulic conductivity excluding boundary flow was much smaller than the hydraulic conductivity employing the conventional determination method. This study also investigated the effects of particle size and surfactant on the hydraulic conductivity. As the particle size increased, the hydraulic conductivity was increased. The hydraulic conductivity was reduced by increasing surfactant concentration. The result showed that the viscosity of fluid significantly affected the determination of hydraulic conductivity.