• 한국축산식품학회지
• /
• 제36권1호
• /
• pp.100-108
• /
• 2016

Microbiological contamination of eggs should be prevented in the poultry industry, as poultry is one of the major reservoirs of human Salmonella. ClO₂ gas has been reported to be an effective disinfectant in various industry fields, particularly the food industry. The aims of this study were to evaluate the antimicrobial effect of chlorine dioxide gas on two strains of Salmonella inoculated onto eggshells under various experimental conditions including concentrations, contact time, humidity, and percentage organic matter. As a result, it was shown that chlorine dioxide gas under wet conditions was more effective in inactivating Salmonella Enteritidis and Salmonella Gallinarum compared to that under dry conditions independently of the presence of organic matter (yeast extract). Under wet conditions, a greater than 4 log reduction in bacterial populations was achieved after 30 min of exposure to ClO₂ each at 20 ppm, 40 ppm, and 80 ppm against S. Enteritidis; 40 ppm and 80 ppm against S. Gallinarum. These results suggest that chlorine dioxide gas is an effective agent for controlling Salmonella, the most prevalent contaminant in the egg industry.

• Mycobiology
• /
• 제44권4호
• /
• pp.338-342
• /
• 2016

The culture filtrate of Lentinula edodes shows potent antimicrobial activity against the plant pathogenic bacteria Ralstonia solanacearum. Bioassay-guided fractionation was conducted using Diaion HP-20 column chromatography, and the insoluble active compound was not adsorbed on the resin. Further fractionation by high-performance liquid chromatography (HPLC) suggested that the active compounds were organic acids. Nine organic acids were detected in the culture filtrate of L. edodes; oxalic acid was the major component and exhibited antibacterial activity against nine different phytopathogenic bacteria. Quantitative analysis by HPLC revealed that the content of oxalic acid was higher in the water extract from spent mushroom substrate than in liquid culture. This suggests that the water extract of spent L. edodes substrate is an eco-friendly control agent for plant diseases.

• 한국식품위생안전성학회지
• /
• 제10권1호
• /
• pp.33-39
• /
• 1995

The antibacterial and antifungal effect of grapefruit seed extract(GFSE) was investigated for its purpose of application to a diverse spectrum of field as sanitizers, disinfectants and preservatives. GFSE showed coparatively high content of such flavoniods as naringin and hesperidin and ascorbic acid. GFSE containing a low level of organic acids is a heavy viscous and water-soluble liquid. As a result of the antimicrobial test of GFSE, Bacillus subtilis and Aspergillus oryzae did not survive at detectable levels when treated with more than 100 ppm of GFSE. The minimum inhibitory concentrations of GFSE for a wide variety of pathogenic and putrefactive bacteria, fungi and yeasts were 100 ppm and 250 ppm, respectively. In the comparable electron micrograph of microbial cells treated with GFSE or not, we could conclude that GFSE destroy microorganisms by disrupting the functions of the cell wall membrane and microbial spores.

• 보존과학연구
• /
• 통권22호
• /
• pp.5-25
• /
• 2001

The germicidal and insecticidal properties of volatile components extracted from star anise(Illicium verum Hooker filius) and clove (Eugenia caryophyllata THUNBERG)were evaluated against five microorganisms and three insects for the purpose of developing biocidal active substances from medicinal plants. The volatile components of star anise and clove showed strong antimicrobial effect against Aspergillus niger, Penicillium funiculosum, Mucor hiemalis, Trichoderma viride, and Aureobasidium pullulans. The extracts of each medicine also showed insecticidal effects against Sitophilusoryzae L., Lyctus linearis GOZE, and Reticulitermes spertus kyushuensis Morimoto. Fumigant toxicities to adult insects were determined. In the case of fumiganttoxicity, the extract of star anise showed 100% mortality against R. spertus, S.oryzae, and L. linearis at rates of $2.5\mu\ell$, $50\mu\ell$, $250\mu\ell$/filter paper, respectively but showed no killing effects by clove. The volatile components of star anise and clove were investigated by means of GC/MS. The main constitute, anethole among 20components from star anise and eugenol among 9 components from clove were identified. The mixture of star anise and clove as the volume ratio of 2 : 1 showed higher properties for antimicrobial and insecticidal effect than each volatile component. A. niger was inhibited by the mixture(125ml/$m^3$) for up to 10 days of exposure. Also, from the result of observing state change of organic materials by volatile extracts of star anise and clove, volatile extracts effects have no effect on natural organic materials of organic cultural properties and can be used as biological control agent. As research contents as above, the insecticidal and germicidal agents from star anise and clove and the mixture of them were more efficient and high level to prevent biological damage for conservation of organic cultural properties. So they may be used in new development of biologicalinsecticidal and germicidal agents for conservation of cultural properties.

• 한국포장학회지
• /
• 제23권3호
• /
• pp.133-141
• /
• 2017

본 논문에서는 신선도 유지 기능을 부여한 새로운 형태의 산화생분해성 필름 개발에 관하여 서술하였다. 생분해 촉매제로 유기 금속염, 유기산, 불포화 지방산등을 함유한 산화생분해성 및 항균신선도기능을 부여한 M/B를 각각 제조한 후, 이를 플라스틱 레진에 첨가하여 항균 및 신선도 기능의 산화생분해 복합필름(AOB film)을 제작하였다. 제조된 항균신선도 A M/B의 항균력 시험은 진탕 플라스크 방법을 사용하여 농도별 시험을 실시하였다. A MB 5% 첨가한 AOB 필름은 별도로 필름 밀착법을 통해 제조하여 실험한 결과, 필름의 항균작용이 매우 우수함을 확인할 수 있었다. 자두를 대상으로 한 신선도 유지기능 평가 결과, A M/B 5% 첨가한 AOB 필름이 대조군 필름에 비하여 신선도 유지 효과가 우수하였다. 또한 산화생분해성을 평가하기 위하여, UV 340 nm로 처리한 필름의 인장강도 및 신장율을 측정한 결과, AOB 필름의 물성 감소율이 우수하였으며, 이는 산화생분해 특성을 갖는 것을 의미한다. 결론적으로 항균 및 신선도 기능의 산화생분해성(AOB) 복합필름은 식품 유통과정에서 발생할 수 있는 식품의 부패를 방지하는 측면에서 긍정적인 효과를 가져올 수 있을 것으로 판단되어진다.

• Journal of Microbiology and Biotechnology
• /
• 제28권8호
• /
• pp.1367-1375
• /
• 2018

Silver nanoparticles have been widely applied for biomedical areas owing to their potent antiviral and antibacterial activities. Synthesis of silver nanoparticles using biomacromolecules is more efficient, environment-friendly, and cost-saving compared with the traditional approach. In this paper, a novel approach was developed to establish a reaction system with $Ag^+-BH4^--sericin$ to synthesize silver nanoparticles conjugated to sericin (AgNPs-Sericin). Sericin could be as a good dispersant and stabilizing agent, which is able to modify nanoscaled AgNPs, the average diameter of which was only $3.78{\pm}1.14nm$ prepared in a 0.3 mg/ml sericin solution. The characterizations of the AgNPs-Sericin were determined by FTIR, thermogravimetry, and XRD analyses. The results showed that the synthesized AgNPs conjugated with sericin as organic phase. Via SAED and XRD analysis, we showed that these AgNPs formed polycrystalline powder with a face-centered cubic structure of bulk metals. Moreover, we investigated the antiviral and antibacterial activities of AgNPs-Sericin, and the results showed that AgNPs-Sericin exhibited potent anti-HIV-1 activity against CCR5-tropic and CXCR4-tropic strains, but no significant cytotoxicity was found toward human genital epithelial cells compared with free silver ions, which are accepted as a commonly used potent antimicrobial agent. Moreover, its antibacterial activity was determined via flow cytometry. The results showed that AgNPs-Sericin could suppress gram-negative (E. coli) and gram-positive (S. aureus) bacteria, but more was potent for the gram-negative one. We concluded that our AgNPs-Sericin could be a potential candidate as a microbicide or antimicrobial agent to prevent sexually transmitted infections.

• 보존과학연구
• /
• 통권29호
• /
• pp.125-136
• /
• 2008

To investigate bioactive materials for development of natural conservative agent on organic cultural heritage, methanol extracts from 17 medicinal plants were screened for antifungal and insecticidal activity against 6 wood rot fungi and adult of Lasioderma serricorne. Antifungal activity of extracts was tested by using paper disc soaking method against wood-rot fungi. Among these extracts, the most significant antimicrobial activity was observed from the extract of Coptis japonica at 5 mg/disc against all wood rot fungi tested. The insecticidal activity of extracts was examined by topical application against L. serricorne adults. The extracts from Asarum sieboldii gave 83.3 % mortality at $25{\mu}g/adult$ for 24hr. From these result, the methanol extract from C. japonica and A. sieboldii described could be useful for conservation of organic cultural heritage against biological deterioration by wood rot fungi and L. serricorne.

• 한국약용작물학회지
• /
• 제22권4호
• /
• pp.270-275
• /
• 2014

This study was conducted to select and develop effect of eco-friendly organic materials for the eco-friendly prevention of Anthracnose occurred in the ginseng. Anthracnose on ginseng is occurred by Colletotrichum gloeosporioides and the crop damage is severe especially from July to August after rainy season. The test results showed that control effect by test products materials on the three years ginseng and four years ginseng field was lower in eco-friendly organic materials than that of chemical pesticide. However, the control effect of bordeaux mixture was higher with 71.3% and 73.8% levels than those of mineral matter, microbial agent, and developed plants extract mixtures (Eugenol, Curcumin, Wood vinegar, etc). On the other hand, three types of developed plants extract mixtures (3 types) showed control effect in a range of from 58.1% to 63.6% against Anthracnose which was higher as compared with plant extract alone and sodium silicate regardless of ages of ginseng. The results of this study would attribute in verifying the control effect of eco-friendly materials against Anthracnose for ginseng through investigating antimicrobial compounds contained in the plants body. Also, it would be used as control method against Anthracnose occurred in ginseng by judging the right control time through monitoring occurrence of disease.

• 접착 및 계면
• /
• 제18권1호
• /
• pp.13-24
• /
• 2017

본 연구에서는 50 wt%의 매우 높은 담지량과 70%의 높은 고형분을 가지면서도 물속이나 다양한 제품에 나노 입자 형태로 분산이 가능한 새로운 형태의 소수성 항균 물질 담지 나노 입자를 제조하였다. 실란 기능화 양친성 고분자 전구체(Alkoxysilane-functionalized Amphiphilic Polymer Precursor; 이하 AAPP)와 다양한 실란 화합물을 이용하여 전형적인 Hydrolytic Sol-Gel 공정으로 제조된 수분산 유-무기 하이브리드 나노 입자들을 제조하고, 이를 이용하여서 나노 침전법을 사용하여서 소수성 항균물질을 고함량으로 담지할 수 있는 새로운 공정으로 소수성 항균물질인 Eugenol이 담지된 유-무기 하이브리드 형태의 나노입자 제조하였다. 나노 입자 제조시 제조 조건의 변화에 따른 나노 입자들의 크기, 담지량, 항균 활성 및 방출거동 등에 영향을 미치는 인자들을 조사하였다. 나노 입자의 종류에 관계없이 Minimal Inhibitory Concentration (MIC)는 50 mg/ml로 동일하였고, 모든 균주에서 99 %에 해당하는 우수한 항균력과 Pseudomonas aeruginosa (PSE)를 제외하고는 2주 이상의 항균 지속력을 나타내었다. 특히, Tetraethoxysilane (TEOS)를 첨가한 경우에는 견고한 무기물 도메인으로 인해 가장 높은 담지량 (50 wt.%)과 서방출 (Sustained release)을 나타내었고, Hexanediol (HD)을 첨가한 경우에는 HD 자체의 항균력과 용매로서의 역할도 하였기 때문에 가장 높은 항균력과 70%의 고형분을 나타내었다.

• 한국산학기술학회논문지
• /
• 제20권5호
• /
• pp.102-108
• /
• 2019

티트리 (Melaleuca alternifolia, Tea tree)는 terpineol-4, cineol, cymene, sesquiterpenes 등을 함유하여 살균 효과와 피부 보습효과를 지니고 있으며 또한, 여드름 염증 완화, 비듬 치료, 통증 완화, 우울증 해소 등의 특성을 가지고 있다. 본 연구에서는 티트리를 유기용매 (Methanol, Dichloromethane, Ethyl acetate) 및 열수추출법을 이용하여 물질을 추출하였으며, 각 추출액에서 나타나는 항균 및 항진균 활성을 확인하였다. 항균 활성 검증은 9종의 병원성 미생물 (Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa, Vibrio parahaemolyticus)을 대상으로 실시하였으며, 항균 활성 검증에 일반적으로 사용되는 disc diffusion법을 이용하여 추출물의 항균 활성을 확인하였다. 항균활성 검증을 통하여 ethyl acetate 분획추출물과 methanol 분획추출물에서 V. parahemolyticus와, S. aureus에 대한 항균활성을 확인하였다. 또한, 최대 활성을 나타내었던 V. parahaemolyticus를 대상으로 각 분획추출물 및 열수추출물의 항균활성을 확인하였으며, 10 mg/mL 농도로 처리하였을 때 99.9 % 이상의 항균활성을 확인하였다. 항진균활성의 경우, 균종에 따른 차이는 있으나 진균류에 대한 약 45시간 이상의 보존력을 확인하였다. 본 연구를 통해서 티트리 열수추출물과 메탄올 추출물의 V. parahaemolyticus에 대한 항균 활성을 확인하였으며, 추가적인 연구를 통해 항균제 및 항진균제로써의 개발 가능성을 확인하고자 한다.