Mycobiology

The Korean Society of Mycology (한국균학회)
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Oxalic Acid from Lentinula edodes Culture Filtrate: Antimicrobial Activity on Phytopathogenic Bacteria and Qualitative and Quantitative Analyses

  • Kwak, A-Min (Graduate School of Future Convergence Technology, Hankyong National University) ;
  • Lee, In-Kyoung (Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Lee, Sang-Yeop (Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Yun, Bong-Sik (Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University) ;
  • Kang, Hee-Wan (Graduate School of Future Convergence Technology, Hankyong National University)
  • Received : 2016.11.17
  • Accepted : 2016.11.28
  • Published : 2016.12.30
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Abstract

The culture filtrate of Lentinula edodes shows potent antimicrobial activity against the plant pathogenic bacteria Ralstonia solanacearum. Bioassay-guided fractionation was conducted using Diaion HP-20 column chromatography, and the insoluble active compound was not adsorbed on the resin. Further fractionation by high-performance liquid chromatography (HPLC) suggested that the active compounds were organic acids. Nine organic acids were detected in the culture filtrate of L. edodes; oxalic acid was the major component and exhibited antibacterial activity against nine different phytopathogenic bacteria. Quantitative analysis by HPLC revealed that the content of oxalic acid was higher in the water extract from spent mushroom substrate than in liquid culture. This suggests that the water extract of spent L. edodes substrate is an eco-friendly control agent for plant diseases.

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References

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