• Title/Summary/Keyword: Orchards

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Phylogenetic and Recombination Analysis of Apple Stem Grooving Virus Isolates from Pears in Korea

  • Nam-Yeon Kim;Rae-Dong Jeong
    • Research in Plant Disease
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    • v.29 no.2
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    • pp.193-199
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    • 2023
  • The apple stem grooving virus (ASGV) is one of the most harmful latent viruses infecting pear orchards worldwide. To examine the genetic diversity of ASGV in Korean pear orchards, the complete coat protein (CP) gene of five ASGV isolates collected from various regions were identified. The five Korean ASGV isolates showed 88-96% nucleotide identity with the 11 isolates worldwide occurring elsewhere in the world. Phylogenetic analysis of five isolates, as well as the previously sequenced isolates, indicated that the ASGV clusters had no correlation with the host or geographical regions of origin. Recombination analysis showed that one of the five Korean isolates is a recombinant, with a recombination site in the CP gene region (nt 532-708). This study is the first report of natural recombination within the CP gene of ASGV isolates from pears grown in Korea.

Seven Unrecorded Species of Ascomycota Isolated from the Rhizosphere Soils of Apple and Pear Trees in Korea

  • Hyeongjin Noh;Hyun Uk Cho;Jun Woo Cho;Seong Jae Ahn;Seong Hwan Kim
    • The Korean Journal of Mycology
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    • v.51 no.4
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    • pp.471-490
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    • 2023
  • Soil is a rich source of saprobic and pathogenic fungi in crop cultivation areas. Compared with that of the fruit trees, scarce information is available regarding the fungi present in orchard soils in Korea. We sampled rhizosphere soils of apple and pear trees from several orchards in Cheongju, Anseong, and Cheonan, Korea. During the processing of soil fungi, seven unrecorded species of the phylum Ascomycota were isolated. These included Acrocalymma walkeri, Clonostachys krabiensis, Coniella vitis, Cosmospora diminuta, Lasiobolidium spirale, Penicillium vallebormidaense, and Pseudothielavia arxii. All the species were identified and described based on morphological characteristics and molecular analysis of the ITS1-5.8S-ITS2 region and the large subunit of nuclear ribosomal RNA gene and partial β-tubulin gene (BenA) sequences. Descriptions and illustrations of the morphological characteristics are provided.

Occurrence Pattern of an Unidentified Moth Captured by Sex Pheromone Trap of the Oriental Fruit Moth, Grapholita molesta, and Its Discriminating Molecular Markers (복숭아순나방(Grapholita molesta) 성페로몬 트랩에 포획된 미동정 나방의 발생패턴과 판별 분자지표)

  • Huh, Hye-Jung;Son, Ye-Rim;Kim, Yong-Gyun
    • Korean journal of applied entomology
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    • v.47 no.3
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    • pp.303-308
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    • 2008
  • An unidentified moth was captured in sex pheromone traps of the oriental fruit moth, Grapholita molesta, especially at spring season in apple orchards and their vicinity. Though the captured males were similar in appearance to G. molesta males, they were easily distinguished by a matted difference in body size. Their occurrence pattern was also similar to that of overwintering G. molesta population from April to May, at which more males were captured in the pheromone traps installed in the vicinity of apple orchards than within apple orchards. After May, they were no longer captured in the pheromone traps. To investigate any larval damage due to this unidentified moth, molecular markers needed to be developed. Four PCR-RFLP markers originated from cytochrome b region of mitochondrial DNA could distinguish this unidentified moth from G. molesta.

Comparative Trapping Efficiency of Five Different Blends of the Two Sex Pheromone Components in Dichocrocis punctiferalis (Lepidoptera: Pyralidae) at Chestnut Orchards in Korea

  • Choi, Kwang Sik;Choi, Won Il;Lee, Chong Kyu;Kim, Young Jae;Jeon, Mun Jang;Shin, Sang Chul
    • Journal of Korean Society of Forest Science
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    • v.97 no.5
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    • pp.555-558
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    • 2008
  • Trapping efficiency of various sex pheromone blends of the peach pyralid moth, Dichocrocis punctiferalis was compared by field study to develop monitoring system with its sex pheromone at chestnut orchards in Korea. Five candidates of the sex pheromone blends used for the field trapping of D. punctiferalis males were 70:30, 75:25, 80:20, 85:15 and 90:10 mixture of (E)-10-hexadecenyl aldehyde (E10-16:Al) and (Z)-10-hexadecenyl aldehyde (Z10-16:Al). All lures were treated with 1 or 2 mg of each blends. During 2 years of field survey, the 75:25 blend was usually the most effective in attracting males among 5 blends tested. For the 2nd generation, the best capturing activity for D. punctiferalis male was observed by lure with 75:25 blend. Both 90:10 and 75:25 blends showed highest efficiency for the 3rd generation. In most cases. lures treated with 1 mg of blend caught more male moths than these treated with 2 mg of blend.

Effect of Sex Pheromone Trap and Bio-insecticides against Large Black Chafer (Holotrichia parallela) in Organic Pear Orchards (배 유기재배 과원에서 성페르몬 트랩과 살충효과 유기농자재가 큰검정풍뎅이 방제에 미치는 영향)

  • Song, Jang-Hoon;Md, Abdul Alim;Choi, Eu-Ddeum;Seo, Ho-Jin
    • Korean Journal of Organic Agriculture
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    • v.26 no.2
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    • pp.245-257
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    • 2018
  • The efficacy of different control techniques against the large black chafer, Holotrichia parallela Motschulsky (Coleoptera: Scarabaeidae), in organic pear (Pyrus pyrifolia) orchards was evaluated. In this study, field trials were conducted in three locations in Korea-Naju, Hampyeong, and Boseong-to evaluate different techniques to suppress these beetles. Pheromone traps, bio-insecticides (Hongmengye and Melchungdaejang), and a combination of the two were applied as treatments. In Naju, Hampyeong, and Boseong, the highest number of adult H. parallela were caught in the control plots (n=45, n=39, and n=20, respectively), while the fewest were caught in the pheromone plus bio-insecticide plot (n=19) in Naju and in the combined treatment plot in Hampyeong (n=10). In Naju, the greatest leaf damage was observed in the control (66%), and in all locations (Naju, Boseong, and Hampyeong), the least damage occurred in the combined treatment plots (42%, 36%, and 24%, respectively). Regarding the tree canopy, the greatest leaf damage was observed in the upper canopy, and less damage was observed in the lower canopy. These results demonstrate that the combination of sex pheromone traps and bio-insecticides can be used to manage H. parallela in organic pear orchards.

Incidences of Leaf Spots and Blights on Kiwifruit in Korea

  • Jeong, In-Ho;Lim, Myoung-Taek;Kim, Gyung-Hee;Han, Tae-Woong;Kim, Hong-Chul;Kim, Min-Ji;Park, Hyun-Su;Shin, Soon-Ho;Hur, Jae-Seoun;Shin, Jong-Sup;Koh, Young-Jin
    • The Plant Pathology Journal
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    • v.24 no.2
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    • pp.125-130
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    • 2008
  • Various kinds of leaf spots and blights were found in kiwifruit (Actinidia deliciosa) orchards on 2006 in Korea. Disease incidences were quite variable between open-field and rain-proof shelter. Rate of diseased leaves was recorded as about 70% at open-field orchards in late season but use of rain-proof vinyl shelters alleviated the disease incidences by 20%. Angular leaf spots appeared at early infection stage on June and several other symptoms were also recognized as the disease developed afterward. On September, brown leaf blights were the most frequent, followed by grayish brown ring spots, silvering gray leaf blights, zonate leaf blights, dark brown ring spots and angular leaf spots at open-field orchards. Four fungal species were frequently isolated from the disease symptoms. Phomopsis sp. was the most predominant fungus associated with the leaf spot and blight symptoms on kiwifruit, followed by Glomerella cingulata, Alternaria alternata and Pestalo-tiopsis sp. Phomopsis sp. was commonly isolated from angular leaf spots, silvering gray leaf blights, and zonate brown leaf blights. G. cingulata, A. alternata and Pestalotiopsis sp. were isolated from grayish brown ring spots (anthracnose), brown ring spots and zonate dark brown leaf blights. Typical symptoms appeared on the wounded and unwounded leaves, which were inoculated by each of Phomopsis sp., G. cingulata, and Pestalotiopsis sp., but A. alternata caused symptoms only on the wounded leaves.

A Survey of Pesticide Residues of Citrus Fruits and Citrus Orchard Soil in Jeju Island - Part 1. Pesticide Residue of Citrus Fruits - (제주도(濟州道)의 감귤(柑橘)및 감귤원(柑橘園) 토양(土壤)에 관(關)한 잔류농약조사(殘留農藥調査) - 제1보(第一報), 감귤중(柑橘中)의 농약잔류(農藥殘留)에 관(關)하여 -)

  • Lee, Kyu-Seung
    • Applied Biological Chemistry
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    • v.23 no.3
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    • pp.178-183
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    • 1980
  • Citrus fruits harvested in 1979 were collected from markets scattered in Jeju and Seogwipo area on Jeju island and also from citrus orchards with five, ten, fifteen, twenty, and thirty years of cultivation history. Organophorous insecticides and organochloro acaricides in the pulp of fruits were analysed by gas liquid chromatograph. It was found that EPN and Kelthane were most significant residues found in the citrus fruits in terms of both level and frequency of occurrence. Among citrus fruits collected from various markets, EPN was detected in 28% of the fruits samples in the range of 0.009 to 0.025 ppm, while Kelthane, at lower level, was found in all citrus fruits in the range of trace to 0.007 ppm. Residue levels of citrus fruits from orchards of various cultivated periods, in general, confirmed those of the fruits collected from commercial markets. However, residues of Kelthane in the citrus fruits tended to increase with orchards having longer cultivation history.

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Comparison and Analysis of Insecticide Resistance of Two Spotted Spider Mite (Tetranychus urficae) among Apple Orchards (사과과수원 점박이응애의 약제 저항성 비교 분석)

  • Lee, Si-Woo;Kim, Kwang-Ho;Park, Chang-Gyoo;Park, Hong-Hyun;Lee, Kwan-Suk;Choi, Byeong-Ryeol;Lee, Sang-Guye
    • The Korean Journal of Pesticide Science
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    • v.14 no.3
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    • pp.266-271
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    • 2010
  • Six insecticides, monocrotophos (24%, Lq), milbemectin (1%, Ec), tebufenpyrad (10%, Ec), propargite (30%, Wp), dicofol (42%, Ec), and fenpropathrin (5%, Ec) were studied for their $LC_{50}s$ to local two-spotted spider mite (TSSM) strains collected at apple orchards in Chungju, Kunwi and Sobo in Korea. Monocrotophos and fenpropathrin were not effective due to resistance development, but milbemectin, tebufenpyrad, dicofol and propargite were effective to TSSM. $LC_{50}$ values to TSSM strains showed the same distribution pattern among apple orchards. However, TSSMs from different apple orchard were clustered into different groups.

Observation on the Fauna of Arthropods form Apple Orchards in Winter in Kyongbuk Province (경북지방 사과원의 동계 절지동물(Arthropoda)상)

  • 이영인;권기면;이순원;류하경;류언하
    • Korean journal of applied entomology
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    • v.36 no.3
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    • pp.231-236
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    • 1997
  • Arthropods of 3 Classes, 19 Orders and 58 Families were collected by the enticing band from the trunk of apple trees in winter in Kyongbuk province. The two spotted spider mite, Tetranychu.v urricae Koch, was dominant species with 53.0%, followed by Eriosomtr lanigerutn Hausmann. 16.7%. Oribatida 13.3% and Collembola 9.3%. Of those, herbivores were majority, followed by some decomposers and few natural enemies. Arthropods of 2 Classes, 12 Orders and 17 Families were observed from the fallen leaves. T. urric,ae 63.1% was dominant, followed by Collembola 13.797~ and other Arthropods. While 2 Classes, 9 Orders and 18 Families were investigated from the soil of apple orchards. They were collembola 37.9%. Oribatida 34.476, T. urticae Ih.l% and others in few numbers. Decomposers were majority, followed by herbivores and few natural enemies.

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