Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Occurrence Pattern of an Unidentified Moth Captured by Sex Pheromone Trap of the Oriental Fruit Moth, Grapholita molesta, and Its Discriminating Molecular Markers

복숭아순나방(Grapholita molesta) 성페로몬 트랩에 포획된 미동정 나방의 발생패턴과 판별 분자지표

  • Huh, Hye-Jung (School of Bioresource Sciences, Andong National University) ;
  • Son, Ye-Rim (School of Bioresource Sciences, Andong National University) ;
  • Kim, Yong-Gyun (School of Bioresource Sciences, Andong National University)
  • 허혜정 (안동대학교 생명자원과학부 식물의학) ;
  • 손예림 (안동대학교 생명자원과학부 식물의학) ;
  • 김용균 (안동대학교 생명자원과학부 식물의학)
  • Published : 2008.09.30
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Abstract

An unidentified moth was captured in sex pheromone traps of the oriental fruit moth, Grapholita molesta, especially at spring season in apple orchards and their vicinity. Though the captured males were similar in appearance to G. molesta males, they were easily distinguished by a matted difference in body size. Their occurrence pattern was also similar to that of overwintering G. molesta population from April to May, at which more males were captured in the pheromone traps installed in the vicinity of apple orchards than within apple orchards. After May, they were no longer captured in the pheromone traps. To investigate any larval damage due to this unidentified moth, molecular markers needed to be developed. Four PCR-RFLP markers originated from cytochrome b region of mitochondrial DNA could distinguish this unidentified moth from G. molesta.

복숭아순나방(Grapholita molesta) 성페로몬 트랩에 미동정 나방이 포획되었다. 이들은 주로 봄에 채집되었으며 형태적으로 복숭아순나방과 유사하나 비교적 몸 크기가 커서 육안으로 쉽게 구별할 수 있다. 이들의 발생 패턴도 4-5월의 복숭아순나방 월동세대 발생과 유사하였다. 이 기간 동안 이들은 사과원 내부 보다는 주로 과수원에서 떨어진 곳에 설치된 페로몬 트랩에 집중적으로 포획되었다. 5월 이후 이들 미동정 나방은 복숭아순나방 페로몬 트랩에 더 이상 포획되지 않았다. 이 미동정 나방과 기존의 복숭아순나방과의 계통 분류학적 판별을 위해 분자마커가 개발되었다. 미토콘드리아 cytochrome b 영역을 기반으로 선발된 4종의 PCR-RFLP 분자지표는 이 미동정 나방과 복숭아순나방을 구분할 수 있었다.

Keywords

References

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