• Biomedical Science Letters
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• v.28 no.1
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• pp.50-57
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• 2022

In this study, we tried to examine the possibility of developing a dental product such as tooth decay prevention and oral hygiene by manufacturing a natural polymer film for oral use. Natural polymer films were prepared from shark byproduct extract (SBE) and gellan gum (GG). As an antibacterial substance, the antibacterial activity of green tea extract against tooth decay-causing bacteria was measured. An film was prepared by adding green tea extract to the composition of SBE and GG. The mechanical, solubility, moisture content and antibacterial function of the prepared film were investigated in detail. Also, the incorporation of GTE into the SBE/GG film improved the physical performance of the film. Increasing the content of GTE improved the antioxidant and antibacterial properties of the film. Formulation of antimicrobial SBE/GG film containing green tea extract was established and these results evidently showed potential for cavity prevention products application.

• Journal of dental hygiene science
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• v.21 no.4
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• pp.251-259
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• 2021

Background: The purpose of this study was to investigate the effects of commercially available calamansi soju and other alcoholic beverages on the microhardness and erosion of resin restorations. Methods: In this study, we evaluated the effects of Calamansi soju, Chamisul fresh, Cass fresh, and Gancia Moscato D'asti on resin restorations. Jeju Samdasoo and Coca-Cola were used as negative and positive controls, respectively. Specimens to be immersed in the beverages were manufactured using composite resin according to the product instructions. In each group, the surface microhardness was measured using a surface microhardness instrument before and after immersion for 5, 15, 30, and 60 minutes. The pattern of change in the surface of the composite resin was observed under a scanning electron microscope (SEM). Paired t-tests, one-way ANOVA, and repeated measures ANOVA were performed to compare the surface microhardness of the specimens, and the Tukey test was used as a post hoc test. Results: The pH of all beverages except Jeju Samdasoo was <5.5, which is the critical pH that can induce erosion. The difference in surface microhardness of the composite resin before and after immersion for 60 minutes was significant in all groups. In particular, the largest change in surface microhardness was observed in the calamansi soju group. In the SEM analysis, loss of composite resin was observed in all groups except the Jeju Samdasoo group, and rough surfaces with pores of various sizes were observed. Conclusion: In this study, all beverages except Jeju Samdasoo decreased the microhardness of the composite resin surface, and it was confirmed that calamansi soju had the greatest change.

• 한국전자현미경학회:학술대회논문집
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• 2005.11a
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• pp.127-129
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• 2005

Bacterial lipopolysaccharide(LPS) is can stimulate the most LPS-responsive cells in the mammalian host. The macrophage response to LPS can protect the host from infection but high levels, contribute to systemic inflammatory response syndrome and destruction of host itself, The previously study, secretory leukocyte pretense inhibitor (SLPI) was known LPS-induced product of macrophage and had the function that antagonizes their LPS-induced activation of pro-inflammation signaling factors. Purpose of this study was to identify the expression of SLPI involving the infection in various cell lines including odontoblast cell line. Therefore, we conducted in vitro researches, which treated the LPS to the MDPC-23, and compared to NIH3T3, RAW264.7. To investigate the expressionof SLPI in mRNA level, the methods was used RT-PCR and western blotting for protein expression of SLPI. Moreover, we performed the scanning electron microscopic (SEM) observation for the morphological change. This work was supported by Korea Science and Engineering Foundation.

• Journal of dental hygiene science
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• v.10 no.3
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• pp.161-165
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• 2010

The aim of this study was to investigate the antimicrobial effect of (-)-epigalocatechin on Fusobacterium nucleatum, Prevotella intermedia, and Porphyromonas gingivalis. To test the antimicrobial effect of (-)-epigalocatechin, the minimum inhibitory concentration (MIC) of against 4 strains of F. nucleatum, 2 strains of P. intermedia, and 2 strains of P. gingivalis was measured by broth dilution method. Time-kill curves were assessed for susceptible bacteria, testing $0{\times}MIC$ (control group), $0.5{\times}MIC$, $1{\times}MIC$, and $2{\times}MIC$ for (-)-epigalocatechin, by counting viable bacteria after 3, 90, 180, 360, 720, 1440 minutes. The MIC of (-)-epigalocatechin was 0.312-0.625, 0.625, and 0.625 mg/ml on the strains of F. nucleatum, P. intermedia, and P. gingivalis, respectively. Time-kill curves demonstrated (-)-epigalocatechin had bactericidal activity on P. intermedia ATCC $25611^T$, P. gingival is ATCC 53978, and F. nucleatum subsp. fusiforme ATCC $51190^T$. The results suggest that (-)-epigalocatechin can be useful in developing the oral hygiene product such as tooth past and gargling solution for the prevention of periodontal diseases.

• Journal of Korean society of Dental Hygiene
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• v.16 no.2
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• pp.233-240
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• 2016

Objectives: The aim of this study was to evaluate the oral health status and behavior in Korean diabetic adults. Methods: The study subjects were 11,840 adults who participated in the fifth Korea National Health and Nutrition Examination Survey(2010-2012). Diabetic status was defined by doctors and fasting blood sugar(FBS) level. Oral health status was assessed by decayed-missing-filled teeth(DMFT), community periodontal index(CPI), periodontal disease, denture needs, limitation of oral function, and chewing difficulty. The oral health behavior was evaluated by oral examination within a year, brushing times a day, and use of auxiliary oral product. The data were analyzed by descriptive analysis, chi-square tests and multiple logistic regression analyses. Results: The prevalence rate of diabetes mellitus diagnosed by doctor and FBS was 8.3% and 10.2%, respectively. The prevalence rate of periodontitis was 25.4%. The proportions of $DMFT{\geq}20$, $CPI{\geq}3$, periodontal disease, denture needs, oral function limitation and chewing difficulty in the confirmed diabetic group by doctor were significantly higher than those of the non-diabetic group(p<0.05). In multiple logistic regression analysis, the adjusted odds ratio(aOR) for periodontal disease(aOR=1.73, 95% CI=1.41-2.12), presence of denture needs(aOR=1.40, 95% CI=1.06-1.84), limited oral function(aOR=1.43, 95% CI=1.15-1.78) and chewing difficulty(aOR=1.41 95% CI=1.13-1.77) in diabetic subjects were significantly higher than those of the non-diabetic subjects. There were similar associations between diabetes defined with FBS and oral health. In oral health behavior, diabetic subjects had significantly lower odds ratios for oral examination(aOR=0.76, 95% CI=0.60-0.98), brushing time ${\geq}2$ times(aOR=0.73, 95% CI=0.57-0.93), and auxiliary oral products(aOR=0.74, 95% CI=0.59-0.94). Conclusions: There was a significant relationship between oral health status and behavior in Korean diabetic adults. Further study is needed to evaluate the underlying mechanisms between diabetes mellitus and oral health status.

• Journal of dental hygiene science
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• v.11 no.4
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• pp.325-332
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• 2011

To get the evidences for decreasing the discomfort, dissatisfaction, and for improving management of the implant prothesis through assessment implant patients' awareness and discomforts about care of the prostheses. The subjects were 300 implant patients who treated in the 20 dental clinics in Pusan, Ulsan and Daegu. The general characteristics, recognition, maintenance, discomfort and dissatisfaction, and oral hygiene status were collected. Level of the discomfort and dissatisfaction was measured by a 5 Likert scale, and analyzed by the equivalent of 100. The life-span of the implants was overestimated, however, periodic recall check and scaling were underwent. The level of discomfort and dissatisfaction was generally good, but many of them (65.3%) complained the food impaction. As a result of the multiple regression analysis, level of the discomfort and dissatisfaction increased in case of more number of the prosthesis ($\beta$=0.864), however decreased in case of more frequent teeth brushing ($\beta$=0.737), more frequent periodic check ($\beta$=-0.737), longer duration from the implantation ($\beta$=-0.090). It is very important for the implant patients to be recognized that the lack of care may shorten the life-span of the implant prosthesis. Therefore, efforts of the continuous oral hygiene care such as, periodic recall check, scaling, teeth brushing, the correct using of additional oral hygiene product, and education would be more emphasized.

• Journal of the Korea Convergence Society
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• v.10 no.7
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• pp.55-62
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• 2019

This study analyzed 467patent for toothbrush that used for oral hearlth, registered for 10years from 2008 to 2017 at KIPRIS which can search patent. Based on the toothbush composition, 8categories were detailed information was subdivided. As a result showed that the most patents were patent for toothbrush bristle, patent for additional function, patent for electronic toothbrush, patent for toothbrush's head, patent for toothbrush in different form, patent for toothbrush's neck, patent for learning of brushing habits, patent for toothbrush's handle. The patent that has a variety of the section was a patent for additional function, the patent for toothbrush bristle was the highest that is 25 in 2012. The only increasing number of patents compared to toothbrush patents, which are decreasing slightly in 2017, was the Patent for learning of brushing habits and the patent for toothbrush's handle. As a result, from 2009, the number of patents for toothbrushes has increased and varied.

• Journal of dental hygiene science
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• v.7 no.3
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• pp.129-133
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• 2007

This study was carried out to see the effect of dental auxiliary product groups being used in case of not being able to brush teeth. This study was executed for 70 students in the Dept. of Dental Hygiene of S Health College, and it was divided into the group which used brushing teeth, the one which used only dental auxiliary products, the one which did onion juice gargle, etc, and measured the effect on the reduction of oral malodor. The experimental results of the study were as below. 1) After onion juice gargle, in the experimental group A which brushed teeth, it showed lasting decrease of oral malodor until 20min since the experiment, and after 30min, the degree of oral malodor started to increase again. 2) After onion juice gargle, the result of the experimental group B which took only supplementary products such as garglin, chewing gum, intaking green tea with roasted rice & brushing a tongue and so on, was as follows. First, in case of experimental group BG which used garglin, until 30 min has passed since the experiment, it showed the lasting reduction of oral malodor as the same trend with the experimental group BG. Second, in chewing gum (experimental group BC), intaking green tea with roasted rice (experimental group BT), and brushing a tongue (experimental group BO), continuous reduction of oral malodor was shown until 10minutes has passed since the experiment, but, after 20minutes, we could see the degree of oral malodor started to increase again. 3) After onion juice gargle, in the experimental group C which only the degree of oral malodor was measured with the passage of time, neither brushing teeth nor using auxiliary goods, until 30min since the experiment, it showed the similar trend of the reduction of oral malodor with other experimental groups, but, it showed high degree of oral malodor, as compared with other experimental groups A and B.

• Journal of dental hygiene science
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• v.6 no.4
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• pp.251-254
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• 2006

In the current research for natural product with antimicrobial effects, various extracts of Mulberry Leaves against microorganisms were evaluated in terms of the minimum inhibitory concentrations(MIC). In general, Candida albicans was more antimicrobial activity than the other microorganisms such as Streptococcus mutans, Staphylococcus epidermis, and Staphylococcus aureus. The maximum activity was exhibited by ethanol extract of the leaves of Mulberry Leaves against Candida albicans (MIC, $1600{\mu}g/ml$). These results suggest that ethanol and water extracts of Mulberry Leaves have a potential antimicrobial activity.

• Journal of Oral Medicine and Pain
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• v.26 no.2
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• pp.107-114
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• 2001

Oral malodor is a problem that traverses history, culture, race and sex. But, up to the present the study on prevalence of oral malodor in normal popualtion is short, especially there is no study on oral malodor prevalence in Korean. In our study, we investigated self-evaluation of oral malodor, self-rating intensity of oral malodor, patients efforts for curing their oral malodor, and degree of satisfaction with their efforts. Investigation was carried out on 368 public Koreans resident in a big city(174 males, 194 females), by method of self-reporting to prepared questionnaire. There was no difference in self-reporting prevalence of oral malodor in sexes(57.5% in male, 58.8% in female), and the prevalence rate was increased with aging. Self-rating intensity of oral malodor in oral malodor patients group(by self-reporting) was ranked in order of slight, weak, middle, strong in both sexes, but the number of self-rating strong female group is significantly larger than that of self-rating strong male group(9.3% in male, 17.9% in female). In question on duration of oral malodor, most subjects answered that their oral malodor was developed in certain circumstances(61.3% in male, 76.3% in female), and oral malodor-developing circumstances was ranked in order of in the early morning, in hunger state, in fatigue, in stressful situations. Subjects effort for curing their oral malodor was ranked in order of more tooth brushing, gum chewing, use of commercial product for oral hygiene, visit dental clinic, and rates of no effort subjects were 15.3% in males and 10.7% in females. The degree of satisfaction with their efforts for curing their oral malodor was very low, only 5.6% of males and 6.6% of females were answered that there were satisfactory improvement in their oral malodor. Collectively, our result revealed that prevalence of oral malodor in public Koreans and their interest in oral malodor were much higher than our anticipation, and that patients satisfaction with improvement in oral malodor was short of their expectations. Considering the life quality of patients suffering from oral malodor, we conclude that developments of more improved diagnostic tools and treatment methods for oral malodor is indispensable in future.